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This meeting took place in 2013
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Meeting Details
Precision Genome Engineering and Synthetic Biology: Designing Genomes and Pathways (C5)
Organizer(s) Dana Carroll and Jef D. Boeke
March 17 - March 22, 2013
Beaver Run Resort • Breckenridge, Colorado USA
Abstract Deadline: November 14, 2012
Late Abstract Deadline: December 19, 2012
Scholarship Deadline: November 14, 2012
Early Registration Deadline: January 17, 2013
Sponsored by Life Technologies Corporation and Sangamo BioSciences, Inc.
CME Information
Summary of Meeting:
Now that we know the sequences of many genomes, from a wide variety of organisms and even from individuals with unique characteristics, many researchers have turned to making intentional modifications of those genomes. Purposes of those modifications include: introducing mutations to evaluate gene function, production of models for human genetic disease, gene correction, radical redesign of genome structure, and the generation of novel cellular networks and pathways. With this conference, we propose to bring together scientists who are: 1) developing tools for precision genome engineering; 2) using those tools for specific purposes in a wide range of organisms, including humans; 3) creating whole synthetic genomes with prospects for wholesale reengineering; 4) engineering cells with novel pathways and properties. Exchange of cutting-edge information regarding approaches, accomplishments and ambitions will augment the capabilities of each group. The meeting will provide an outstanding forum for students, postdoctoral fellows, and senior investigators who envision careers in genome and pathway design.
CME Information
Now that we know the sequences of many genomes, from a wide variety of organisms and even from individuals with unique characteristics, many researchers have turned to making intentional modifications of those genomes. Purposes of those modifications include: introducing mutations to evaluate gene function, production of models for human genetic disease, gene correction, radical redesign of genome structure, and the generation of novel cellular networks and pathways. With this conference, we propose to bring together scientists who are: 1) developing tools for precision genome engineering; 2) using those tools for specific purposes in a wide range of organisms, including humans; 3) creating whole synthetic genomes with prospects for wholesale reengineering; 4) engineering cells with novel pathways and properties. Exchange of cutting-edge information regarding approaches, accomplishments and ambitions will augment the capabilities of each group. The meeting will provide an outstanding forum for students, postdoctoral fellows, and senior investigators who envision careers in genome and pathway design.
Conference Program Print | View meeting in 12 hr (am/pm) time
SUNDAY, MARCH 17
08:15—09:15
Keynote Address
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Jef D. Boeke,
Johns Hopkins University School of Medicine, USA
09:15—11:30
Pathway Engineering
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*
Frances H. Arnold,
California Institute of Technology, USA
Sang Yup Lee,
Korea Advanced Institute of Science and Technology (KAIST), South Korea
Redesigning Cellular Metabolism for Chemicals Production
Redesigning Cellular Metabolism for Chemicals Production
Stephen B. del Cardayre,
LS9, Inc., USA
Scalpels to Chainsaws: A Practical Tool Belt for Industrial Synthetic Biology
Scalpels to Chainsaws: A Practical Tool Belt for Industrial Synthetic Biology
Ron Weiss,
Massachusetts Institute of Technology, USA
Synthetic Biology: From Parts to Modules to Therapeutic Systems
Synthetic Biology: From Parts to Modules to Therapeutic Systems
Lauren R. Polstein,
Duke University, USA
Short Talk: Light-Inducible Genetic Control in Human Cells
Short Talk: Light-Inducible Genetic Control in Human Cells
17:00—19:30
Zinc-Finger Nucleases and TALENs
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*
Philip D. Gregory,
Sangamo BioSciences, Inc., USA
Dana Carroll,
University of Utah School of Medicine, USA
Parameters Affecting ZFN- and TALEN-Mediated Gene Targeting
Parameters Affecting ZFN- and TALEN-Mediated Gene Targeting
Thomas Lahaye,
Ludwig Maximilians University Munich, Germany
TALE Like Proteins from Ralstonia solanacearum and their Potential to Improve Current TALE Technology
TALE Like Proteins from Ralstonia solanacearum and their Potential to Improve Current TALE Technology
Marcus B. Noyes,
Princeton University, USA
Short Talk: A Comprehensive Survey of the Cys2His2 Zinc Finger Domain and Selection of Fine-Tuned Specificity
Short Talk: A Comprehensive Survey of the Cys2His2 Zinc Finger Domain and Selection of Fine-Tuned Specificity
08:00—11:00
Engineering Genomes
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*
Edward M. Rubin,
Lawrence Berkeley National Laboratory, USA
Daniel G. Gibson,
J. Craig Venter Institute, USA
Building a Synthetic Cell and Advancing Synthetic Genomics Technology
Building a Synthetic Cell and Advancing Synthetic Genomics Technology
Jef D. Boeke,
Johns Hopkins University School of Medicine, USA
Synthetic Yeast Genome
Synthetic Yeast Genome
Ahmad S. Khalil,
Boston University, USA
Short Talk: A Synthetic Biology Framework for Programming Eukaryotic Transcription Functions
Short Talk: A Synthetic Biology Framework for Programming Eukaryotic Transcription Functions
14:30—16:30
Workshop 1: Tools ‘n Fuels
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Virginia W. Cornish,
Columbia University, USA
Jonathan R. Karr,
Stanford University, USA
Biological Design and Genome Optimization Using Whole-Cell Models
Biological Design and Genome Optimization Using Whole-Cell Models
Todd Peterson,
Life Technologies, USA
Genetic Design, Synthesis and Assembly Tools for Synthetic Biology Engineering
Genetic Design, Synthesis and Assembly Tools for Synthetic Biology Engineering
Klavs Riishede Hansen,
Evolva Biotech A/S, Denmark
Exploring Chemical Diversity by Creative Combination of Metabolic Pathways
Exploring Chemical Diversity by Creative Combination of Metabolic Pathways
Leslie A. Mitchell,
Johns Hopkins University School of Medicine, USA
The Telomerator: A New Tool for Chromosome Engineering
The Telomerator: A New Tool for Chromosome Engineering
Neha Munjal,
International Center for Genetic Engineering & Biotechnology (ICGEB), India
Native Pathway Engineering in E. coli for Production of Bioethanol from Pentose and Hexose Sugars
Native Pathway Engineering in E. coli for Production of Bioethanol from Pentose and Hexose Sugars
Thomas Armel,
Booz Allen Hamilton, USA
Living Foundries: Tools and Infrastructure for Engineering Biology
Living Foundries: Tools and Infrastructure for Engineering Biology
Vítor Martins dos Santos,
Wageningen University, Netherlands
Streamlining and Reprogramming a Bacterial Catalyst for the Production of Bulk and Fine Chemicals
Streamlining and Reprogramming a Bacterial Catalyst for the Production of Bulk and Fine Chemicals
17:00—19:00
Genome Engineering in Humans and Other Animals
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*
Toni Cathomen,
University Medical Center Freiburg, Germany
Scott C. Fahrenkrug,
University of Minnesota, USA
Engineering the Genomes of Food Animals with TALENs
Engineering the Genomes of Food Animals with TALENs
Angelo Lombardo,
San Raffaele Telethon Institute for Gene Therapy, Italy
Gene Targeting in Human Stem Cells by ZFNs
Gene Targeting in Human Stem Cells by ZFNs
Julien Valton,
Cellectis SA, France
Short Talk: XPC Gene Therapy Using Nuclease-Assisted Targeted Approaches
Short Talk: XPC Gene Therapy Using Nuclease-Assisted Targeted Approaches
Philip D. Gregory,
Sangamo BioSciences, Inc., USA
ZFN-Edited CD4+ T Cells for HIV/AIDS Therapy: Phase 1 Trials of SB-728-T in HIV-Infected Subjects
ZFN-Edited CD4+ T Cells for HIV/AIDS Therapy: Phase 1 Trials of SB-728-T in HIV-Infected Subjects
08:00—11:15
Design in Synthetic Biology
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*
Ron Weiss,
Massachusetts Institute of Technology, USA
Tanja Kortemme,
University of California, San Francisco, USA
Computational Design of Proteins, Interactions and Machines with New Functions
Computational Design of Proteins, Interactions and Machines with New Functions
Petra Schwille,
Max Planck Institute of Biochemistry, Germany
Design Features of Protein Clockworks
Design Features of Protein Clockworks
Jason W. Chin,
MRC, Laboratory of Molecular Biology, UK
Reprogramming the Genetic Code
Reprogramming the Genetic Code
Yaakov (Kobi) Benenson,
ETH Zürich, Switzerland
Biological Computing Meets Synthetic Biology: New Ways to Control Cells
Biological Computing Meets Synthetic Biology: New Ways to Control Cells
Douglas Densmore,
Boston University, USA
Clotho - CAD for Synthetic Biology
Clotho - CAD for Synthetic Biology
14:30—16:30
Workshop 2: Designer Engineering of Genomes
David R. Edgell,
University of Western Ontario, Canada
Monomeric Tev-TAL Nucleases for Genome Engineering Applications
Monomeric Tev-TAL Nucleases for Genome Engineering Applications
Shengdar Q. Tsai,
Massachusetts General Hospital, USA
Precise Editing of Human Genes Directed by ssDNA Oligonucleotides and TALENs
Precise Editing of Human Genes Directed by ssDNA Oligonucleotides and TALENs
Nicholas Baltes,
University of Minnesota, Twin Cities, USA
In Planta Gene Targeting with Viruses
In Planta Gene Targeting with Viruses
*
Gregory D. Davis,
Sigma-Aldrich, USA
Enrichment of Genome Edited Cell Populations by Transient Co-Expression of Fluorescent Proteins and Zinc Finger Nucleases
Enrichment of Genome Edited Cell Populations by Transient Co-Expression of Fluorescent Proteins and Zinc Finger Nucleases
Kamila S. Gwiazda,
University of Washington, USA
Coupling Endonucleases with DNA End-Processing Enzymes to Promote Gene Disruption
Coupling Endonucleases with DNA End-Processing Enzymes to Promote Gene Disruption
Prashant Mali,
Harvard Medical School, USA
RNA-Guided Human Genome Engineering
RNA-Guided Human Genome Engineering
Andrew C. Mercer,
The Scripps Research Institute, USA
Engineering a Framework for Active TALE Recombinases
Engineering a Framework for Active TALE Recombinases
Daniel Wattendorf,
Defense Advanced Research Projects Agency (DARPA), USA
Towards a Genetic Jump Drive
Towards a Genetic Jump Drive
17:00—19:15
Targeting with Transposons, Integrases, Meganucleases and CRISPRs
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*
Daniel Voytas,
University of Minnesota, USA
Nancy L. Craig,
HHMI/Johns Hopkins University School of Medicine, USA
piggyBac Transposons for Genome Engineering
piggyBac Transposons for Genome Engineering
Michele P. Calos,
Stanford University School of Medicine, USA
Integrase-Mediated Gene Addition to Generate Corrected Induced Pluripotent Stem Cells for Cell Therapy of Muscular Dystrophy
Integrase-Mediated Gene Addition to Generate Corrected Induced Pluripotent Stem Cells for Cell Therapy of Muscular Dystrophy
Barry L. Stoddard,
Fred Hutchinson Cancer Research Center, USA
Engineered Homing Endonucleases for Gene Targeting via Single- and Double-Strand Breaks
Engineered Homing Endonucleases for Gene Targeting via Single- and Double-Strand Breaks
Rachel E. Haurwitz,
Caribou Biosciences, Inc., USA
Short Talk: RNA-Guided Site-Specific Genome Editing Using Cas9
Short Talk: RNA-Guided Site-Specific Genome Editing Using Cas9
Jin-Soo Kim,
Seoul National University, South Korea
Short Talk: Genome Editing in Cells and Organisms with ZFNs, TALENs, and RNA-Guided Endonucleases (RGENs)
Short Talk: Genome Editing in Cells and Organisms with ZFNs, TALENs, and RNA-Guided Endonucleases (RGENs)
08:00—11:00
Programming Organismal Behavior
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*
Yaakov (Kobi) Benenson,
ETH Zürich, Switzerland
Christopher Voigt,
Massachusetts Institute of Technology, USA
Programming Devices and Networks
Programming Devices and Networks
Jeff Hasty,
University of California, San Diego, USA
Engineered Gene Circuits: From Oscillators to Synchronized Clocks and Biopixels
Engineered Gene Circuits: From Oscillators to Synchronized Clocks and Biopixels
Virginia W. Cornish,
Columbia University, USA
Expanding the Synthetic Capabilities of Yeast
Expanding the Synthetic Capabilities of Yeast
June I. Medford,
Colorado State University, USA
Synthetic Signal Transduction and Design of New Traits
Synthetic Signal Transduction and Design of New Traits
Timothy K. Lu,
Massachusetts Institute of Technology, USA
Short Talk: Scalable Analog and Digital Platforms for Programming Living Cells
Short Talk: Scalable Analog and Digital Platforms for Programming Living Cells
16:00—18:00
Engineering Biofuel and Specialty Chemical Production
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*
Timothy K. Lu,
Massachusetts Institute of Technology, USA
Xiaoxia Nina Lin,
University of Michigan, USA
Short Talk: Engineering Synthetic Microbial Consortia for Consolidated Bioprocessing
Short Talk: Engineering Synthetic Microbial Consortia for Consolidated Bioprocessing
Jack D. Newman,
Amyris, USA
Engineering Biofuel and Specialty Chemical Platforms in Yeast
Engineering Biofuel and Specialty Chemical Platforms in Yeast
*Session Chair †Speaker invited, not yet responded.
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