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This meeting took place in 2015



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Precision Genome Engineering and Synthetic Biology (A1)


Organizer(s) Philip D. Gregory, Jennifer A. Doudna and Ron Weiss
January 11—16, 2015
Big Sky Resort • Big Sky, Montana USA
Discounted Abstract Deadline: Sep 22, 2014
Abstract Deadline: Oct 14, 2014
Scholarship Deadline: Sep 22, 2014
Discounted Registration Deadline: Nov 11, 2014

Sponsored by Agilent Technologies, Inc., Editas Medicine, Regeneron Pharmaceuticals, Inc., Sangamo BioSciences, Inc. and Thermo Fisher Scientific Inc.

Summary of Meeting:
The fields of precision genome engineering and synthetic biology are undergoing a revolution. Building on the rich resource of published genome sequences from a range of organisms (including humans), new enzymatic tools for the purposeful and precise modification of these genomes in vivo are enabling researchers to interrogate the complexity of genetic variation and determine the cause or consequence of mutations with unprecedented speed. Together with rapidly developing tools in synthetic biology, it is now possible to introduce mutations to evaluate gene function, generate models of human genetic disease, perform gene correction, chromosomally integrate synthetic gene networks that perform complex regulatory functions, and redesign genome structure or build whole genomes from scratch. This conference will bring together scientists who are: 1) Developing tools for precision genome engineering; 2) Using those tools for specific purposes in a wide range of organisms, including humans; 3) Engineering cells with novel pathways and properties; and 4) Creating whole synthetic genomes with prospects for wholesale reengineering. This meeting should attract world experts in the development and application of synthetic biology and gene engineering – two fields that could benefit directly from the exchange of ideas, methods, problems and solutions to usher in a new era of investigational biology. In addition, the meeting will provide an outstanding forum for students, postdoctoral fellows and senior investigators who envision careers in genome and pathway design.

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No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 12 hr (am/pm) time


SUNDAY, JANUARY 11

16:00—20:00
Arrival and Registration

Lower Atrium
18:00—20:00
Welcome Mixer
No registration fees are used to fund alcohol served at this function.

Jefferson

MONDAY, JANUARY 12

07:00—08:00
Breakfast

Gallatin
08:00—08:15
Welcome Remarks
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
Philip D. Gregory, Bluebird Bio, USA

Jennifer A. Doudna, HHMI/University of California, Berkeley, USA

Ron Weiss, Massachusetts Institute of Technology, USA

08:15—09:15
Keynote Address
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* Jennifer A. Doudna, HHMI/University of California, Berkeley, USA

Dana Carroll, University of Utah School of Medicine, USA
Mutations on Demand – A Perspective on Genome Editing

09:15—11:45
Editing and Control at Whole-Genome Scale: The Challenge
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* Blake Wiedenheft, Montana State University, USA

Jennifer A. Doudna, HHMI/University of California, Berkeley, USA
CRISPR-Cas Genome Surveillance: From Basic Biology to Transformative Technology

Feng Zhang, Broad Institute of MIT and Harvard University, USA
Development and Applications of CRISPR-Cas9 for Genome Editing

J. Keith Joung, Massachusetts General Hospital, USA
Targeted Genome and Epigenome Editing Using CRISPR-Cas and TALE Technologies

Haoquan Wu, Texas Tech University Health Sciences Center, USA
Short Talk: A CRISPR-Cas9-Based Screen for Human Genes Essential for West Nile Virus-Induced Cell Death

Sidi Chen, Massachusetts Institute of Technology, USA
Short Talk: Modeling Competition of Multiple Cancer Mutations in vivo Using a Conditional CRISPR-Cas9 Mouse

09:40—10:00
Coffee Break

Lower Atrium
11:45—13:00
Poster Setup

Gallatin
13:00—22:00
Poster Viewing

Gallatin
11:45—14:30
On Own for Lunch

14:30—16:30
Workshop 1: Advances in Genome Editing

Jefferson/Madison
* Andy May, Caribou Biosciences, Inc., USA

Yinan Kan, University of Minnesota, USA
The Mechanism of Cas9/CRISPR-Mediated Gene Targeting Using ssODNs: Cleavases, Nickases and Paired-Nickases

Mehmet Fatih Bolukbasi, University of Massachusetts Medical School, USA
Improved Genome Targeting Precision Using a Chimeric Cas9 Nuclease

Tilmann Bürckstümmer, Haplogen Genomics, Austria
Evidence from more than 700 Genome Editing Experiments Using CRISPR/Cas – Lessons Learned

Samuel H. Sternberg, University of California, Berkeley, USA
Rational Design of Split-Cas9 for Genome Engineering

Paul D. Donohoue, Caribou Biosciences, Inc., USA
Modulation of Cas9 Activity through Single Guide RNA Engineering

Emily Crawford, University of California, San Francisco, USA
Editing the Plasmodium falciparum Genome by Transfection of Cas9-sgRNA Ribonucleoprotein

Mohammad A. Mandegar, Gladstone Institutes, USA
A Doxycycline-Inducible CRISPR Inhibition iPS Cell Reporter Line for Cardiac Disease Modeling

Pankaj K. Mandal, Harvard University/Boston Children's Hospital, USA
CRISPR/Cas9 Mediated Ablation of Clinically Relevant Genes in Human CD34+ HSPCs and CD4+ T Cells

16:30—17:00
Coffee Available

Lower Atrium
17:00—19:00
Design Principles and Practice of Basic Parts to Large Scale Circuits
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* Jacquin Niles, Massachusetts Institute of Technology, USA

Christopher Voigt, Massachusetts Institute of Technology, USA
Programmable Cell Technologies

Adam P. Arkin, University of California, Berkeley, USA
Knowledge, Context and Process: Building a Foundational Infrastructure for Engineering Cells for Use in an Uncertain World

Benjamin Jester, University of Washington, USA
Short Talk: Biosensors Engineered from Conditionally Stable Ligand-Binding Domains

Patrick Guye, Massachusetts Institute of Technology, USA
Short Talk: Engineering a Self-Organizing Liver Bud-Like Hematopoietic Niche from Human Pluripotent Stem Cells

19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.

Gallatin
19:30—22:00
Poster Session 1

Gallatin

TUESDAY, JANUARY 13

07:00—08:00
Breakfast

Gallatin
08:00—11:15
Beyond On-and-Off: Analog Circuits, Non-Transcriptional Regulation
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* June I. Medford, Colorado State University, USA

Timothy K. Lu, Massachusetts Institute of Technology, USA
Synthetic Analog Computing and Memory in Living Cells

Ron Weiss, Massachusetts Institute of Technology, USA
Mammalian Synthetic Biology: From Parts to Modules to Therapeutic Systems

Andrew D. Ellington, University of Texas at Austin, USA
Targetrons as Genome Engineering Tools

Kathy Y. Wei, Stanford University, USA
Short Talk: Programmable RNA-Based Tools for Conditional Control over Gene Expression

Daniel Woodsworth, Genome Sciences Centre, Canada
Short Talk: A Lymphocyte-Based Cell-to-Cell Therapeutic Delivery System

Alexander S. Beliaev, Pacific Northwest National Laboratory, USA
Short Talk: Engineering RNA Aptamer-Based Gene Circuits for Control of Microbial Interactions

09:20—09:40
Coffee Break

Lower Atrium
11:15—13:00
Poster Setup

Gallatin
13:00—22:00
Poster Viewing

Gallatin
11:15—17:00
On Own for Lunch

16:30—17:00
Coffee Available

Lower Atrium
17:00—19:00
Found in Translation: Disease Modeling in Cells and Animals
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* Paula M. Cannon, University of Southern California, Keck School of Medicine, USA

Jennifer E. Phillips-Cremins, University of Pennsylvania, USA
Unraveling 3-D Folding Principles of the Pluripotent Genome

Howard J. Jacob, HudsonAlpha Institute for Bioltechnology, USA
Genome-Edited Rats as Models for Human Disease

Jacquin Niles, Massachusetts Institute of Technology, USA
Gene Regulatory Devices for Malaria

Marcus C.S. Lee, Columbia University, USA
Short Talk: CRISPR-Mediated Genome Editing and Gene Regulation in Plasmodium falciparum Malaria Parasites

19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.

Gallatin
19:30—22:00
Poster Session 2

Gallatin

WEDNESDAY, JANUARY 14

07:00—08:00
Breakfast

Gallatin
08:00—11:15
The Genome-Edited Cell as a Window onto Biological Mechanism
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* Dana Carroll, University of Utah School of Medicine, USA

Maria Jasin, Memorial Sloan-Kettering Cancer Center, USA
Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining

Danilo Maddalo, Memorial Sloan-Kettering Cancer Center, USA
Short Talk: In vivo Engineering of Oncogenic Chromosomal Rearrangements Using the CRISPR/Cas9 System

Yannick Doyon, Laval University, Canada
Short Talk: Charting the Path from Oncogenic Rearrangement to Epigenomic Outcome via Genome Editing

Dirk Hockemeyer, University of California, Berkeley, USA
Elucidating Telomerase Function in Human Tumor and Stem Cell Biology Using Genetically Defined Cell Models

Laura Vian, NIAMS, National Institutes of Health, USA
Short Talk: Using Genome Editing Techniques to Uncover Rules of “CTCF Code”

Danwei Huangfu, Memorial Sloan-Kettering Cancer Institute, USA
Short Talk: hESC-Based Functional Human Genetics for Studying Pancreatic Development and Diabetes

Michele P. Calos, Stanford University School of Medicine, USA
Genome Engineering in a Stem Cell Therapy for Muscular Dystrophy

09:20—09:40
Coffee Break

Lower Atrium
11:15—13:00
Poster Setup

Gallatin
13:00—22:00
Poster Viewing

Gallatin
11:15—17:00
On Own for Lunch

16:30—17:00
Coffee Available

Lower Atrium
17:00—19:00
Health 2025: On the Path to the Clinic
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* Howard J. Jacob, HudsonAlpha Institute for Bioltechnology, USA

Thomas Armel, Quantitative Scientific Solutions, USA
Talk Title to be Announced

Bruce R. Conklin, Gladstone Institutes and UCSF, USA
Precise Genome Engineering in Human iPS Cells for Disease Modeling and Therapy

Paejonette Jacobs, NIAID, National Institutes of Health, USA
Short Talk: A Novel Cure Strategy for WHIM Syndrome by Targeted Genome Engineering of CXCR4 to Convey a Competitive Advantage to Hematopoietic Stem Cells

Paula M. Cannon, University of Southern California, Keck School of Medicine, USA
Using Targeted Nucleases to Create an HIV-Resistant Immune System

19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.

Gallatin
19:30—22:00
Poster Session 3

Gallatin

THURSDAY, JANUARY 15

07:00—08:00
Breakfast

Gallatin
08:00—11:15
Protein, Enzyme and Metabolic Engineering: "Tools and Fuels"
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* Timothy K. Lu, Massachusetts Institute of Technology, USA

John Glass, J. Craig Venter Institute, USA
Building and Using a Minimal Microbial Cell to Understand the 1st Principles of Cellular Life

David Baker, University of Washington, USA
Design of Novel Protein Structures, Functions and Assemblies

Kristala Jones Prather, Massachusetts Institute of Technology, USA
Metabolite Valves: Dynamic Control of Metabolic Flux for Pathway Engineering

Michelle C. Chang, University of California, Berkeley, USA
Designing New Biosynthetic Pathways for in vivo Cellular Production of Biofuels and Pharmaceuticals

Andrew Horwitz, Amyris, Inc., USA
Short Talk: One-Step Engineering of Complete Metabolic Pathways in Yeasts Using CRISPR-Cas9

David F. Savage, University of California, Berkeley, USA
Short Talk: Rapid Construction of Metabolite Biosensors

09:20—09:40
Coffee Break

Lower Atrium
11:15—14:30
On Own for Lunch

14:30—15:30
Workshop 2: BioCAD Tools: Design and Mapping to Biological Circuits

Jefferson/Madison
* Douglas Densmore, Boston University, USA

Hyun-Seob Song, Pacific Northwest National Laboratory, USA
Dynamic Metabolic Modeling as a Design Tool for Synthetic Microbial Consortia

Kevin Clancy, Thermo Fisher Scientific, USA
Vector NTI Express Designer - A Synthetic Biology Design Software

Kamalakar Chatla, Mayo Clinic, USA
The Zebrafish Genome Editing Toolkit for Precision Medicine

15:30—16:30
BioCAD Hands-On Tutorial

Jefferson/Madison
Douglas Densmore, Boston University, USA

Victor Dillard, Desktop Genetics, UK

16:30—17:00
Coffee Available

Lower Atrium
17:00—18:45
Earth 2025: Edited Life Forms for a Better World
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
* John Glass, J. Craig Venter Institute, USA

June I. Medford, Colorado State University, USA
Plant Synthetic Biology for a Sustainable Future

Zhan-Bin Liu, Dupont Pioneer, USA
Short Talk: Genome Engineering in Soybean

Nicholas D. Bonawitz, Dow AgroSciences, LLC, USA
Short Talk: Targeted Transgene Insertion in Maize via Non-Homologous End Joining at a Zinc Finger Nuclease-Induced Double Strand Break

Scott C. Fahrenkrug, Recombinetics, Inc., USA
Accelerated Breeding of Livestock by Genome Editing

18:45—19:15
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Meeting has ended...abstracts no longer viewable online. Purchase an Abstract Book from this meeting

Jefferson/Madison
19:15—20:15
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.

Gallatin
20:00—23:00
Entertainment
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Gallatin

FRIDAY, JANUARY 16

 
Departure


*Session Chair †Invited, not yet responded.



Keystone Symposia thanks our Sponsors for generously supporting this meeting:

Agilent Technologies, Inc. Editas Medicine
Regeneron Pharmaceuticals, Inc. Sangamo BioSciences, Inc.
Thermo Fisher Scientific Inc.
 

We gratefully acknowledge support for this conference from:


Directors' Fund


These generous unrestricted gifts allow our Directors to schedule meetings in a wide variety of important areas, many of which are in the early stages of research.

Click here to view all of the donors who support the Directors' Fund.



We gratefully acknowledge additional support for this conference from:


DuPont Pioneer

Dharmacon, part of GE Healthcare Sigma Life Science

We gratefully acknowledge the generous grant for this conference provided by:


National Science Foundation (NSF)

Grant No. MCB-1449714

The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the National Science Foundation; nor does mention of trade names, commercial practices, or organizations imply endorsement by the U.S. Government.


We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements:



Sangamo BioSciences, Inc.


We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Genetic Engineering & Biotechnology News

Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:


Click here to view more of these organizations


If your organization is interested in joining these entities in support of Keystone Symposia, please contact: Sarah Lavicka, Assistant Director of Development, Email: sarahl@keystonesymposia.org,
Phone:+1 970-262-2690

Click here for more information on Industry Support and Recognition Opportunities.

If you are interested in becoming an advertising/marketing in-kind partner, please contact:
Yvonne Psaila, Director, Marketing and Communications, Email: yvonnep@keystonesymposia.org,
Phone:+1 970-262-2676