Keystone Symposia

Structural Genomics: Expanding the Horizons of Structural Biology (J2)

Organizer(s): Ian A. Wilson, Kurt Wüthrich, Soichi Wakatsuki and Christine A. Orengo
January 8 - 13, 2010
Beaver Run Resort · Breckenridge, Colorado
Abstract Deadline: September 14, 2009
Late Abstract Deadline: October 12, 2009
Scholarship Deadline: September 14, 2009
Early Registration Deadline: November 9, 2009

Supported by The Directors' Fund

Joint meeting: Structural Biology (J1)
NOTE: Registration for meeting allows attendance at joint meeting (pending space availability).

This meeting took place in the 2010 season.

For a complete list of the meetings for the upcoming/current season,
see our meeting list, or search for a meeting.

Summary of Meeting
Structural Biology continues to be one of the most prolific and informative ways to make biological and biomedical discoveries at the turn of the millennium, and provides fundamental molecular level insights into the underlying biological and biochemical functions. Structural genomics has been at the forefront of these developments as a major contributor to the advances of high throughput (HT) technologies and methodologies that span the gene to structure process by both crystallography and NMR. In parallel, the rapid expansion of the genome sequencing projects is substantially increasing the complexity and size of the known protein universe, and providing structural biologists with a wealth of new opportunities and challenges to explore, the diversity of biological macromolecules and their relationships to, function, evolution and disease. The goal of this Keystone Symposia meeting is to explore how we can best harness the current approaches from high throughput structural genomics to tackle present and future challenges in structural biology and how we can directly apply these methods to topical and important problems in structural, molecular, cellular and chemical biology. We will focus on approaches that are applicable not only for large consortia, but also for single investigator laboratories and smaller scale projects. Using such methodologies and technologies, we can then keep pace with the genome sequencing efforts and continue to make significant contributions to the constantly increasing discoveries on the intricacies and vagaries of complex biological processes – such as mammalian proteomes, microbial pathogens and cellular and signaling networks / systems. In this way, structural biology will continue to lead the way in making innovative discoveries in biological, chemical, and biomedical research.

Friday, January 8
3:00 - 7:30 PM	Registration	Foyer
6:15 - 7:15 PM	Refreshments	Foyer
7:15 - 7:30 PM	Orientation for New Attendees and New Investigators Keystone Symposia's Chief Scientific Officer, Dr. Andy Robertson, to lead discussion in a "What to Expect during your Attendance" for interested delegates.	Peaks 1-5
7:30 - 8:30 PM	Keynote Session (Joint)	Peaks 1-5
	* Ian A. Wilson, The Scripps Research Institute
	* Alasdair C. Steven, National Institute of Arthritis, Musculoskeletal and Skin Diseases, NIH


	William E. Moerner, Stanford University Single-Molecule Approaches to Biomolecular Dynamics and Imaging of Cellular Superstructures
Saturday, January 9
7:00 - 8:00 AM	Breakfast	Summit Gallery
8:00 - 11:15 AM	Pushing the Limits of Structural Biology I: New and Innovative Methods (Joint)	Peaks 1-5
	* Kurt Wüthrich, ETH Zurich/The Scripps Research Institute
	Andrej Sali, University of California, San Francisco Integrative Determination of Macromolecular Structures
	John A. Tainer, The Scripps Research Institute Short Talk: Proteomics Scale X-ray Solution Scattering (SAXS) to Shape Interactomes
	Ad Bax, NIDDK, National Institutes of Health Combining Novel Solution NMR Approaches with SAXS
	Wah Chiu, Baylor College of Medicine Seeing Protein Backbone and Side-Chains in Molecular Machines by Cryo-EM
	Daniel J. Müller, University of Technology Dresden Force Probing How Membrane Proteins Regulate Molecular Mechanics of Cell Rounding
	Graham Johnson, The Scripps Research Institute Short Talk: Automated Visualization of Subcellular Environments
9:20 - 9:40 AM	Coffee Break	Foyer
11:15 AM - 1:00 PM	Poster Setup	Breckenridge Ballroom
11:15 AM -	On Own for Lunch and Recreation
1:00 - 10:00 PM	Poster Viewing	Breckenridge Ballroom
2:30 - 4:30 PM	Workshop 1: Advances in Methodologies and Tools for Structural Biology This workshop will focus on new methodologies, technologies, instrumentation, devices, software and innovations for production, characterization, structure determination and analysis of biological macromolecules, including servers, databases and repositories.	Peak 5
	* Helen M. Berman, Rutgers University
	* Andy May, Fluidigm Corporation
	George N. Phillips, Jr., University of Wisconsin, CESG Use of a Cell-Free Protein Production Platform for X-Ray Crystallography
	Cory J. Gerdts, Emerald BioSystems Nanovolume Optimization of Protein Crystal Growth Using the Microcapillary Protein Crystallization System
	Andrei Kouranov, Protein Data Bank PepcDB (Protein Expression Purification Crystallization DataBase): A Tool for Experimental Design
	Paolo Rossi, Rutgers University Synergies of X-ray, NMR, and Rosetta in Protein Structure Determination
	Peter Minary, Stanford University Conformational Optimization with Natural Degrees of Freedom: A Novel Stochastic Chain Closure Algorithm and its Applications
	Joshua M. Gilmore, Stowers Institute for Medical Research Determining Protein Complex Architecture using a Probabilistic Deletion Network Derived from Quantitative Proteomics
	Gyorgy Babnigg, Argonne National Laboratory Comparison of High-Throughput Techniques for the Expression of Protein Complexes

	Wladek Minor, University of Virginia Application of the Ligand Screening Module in HKL-3000 for High-Throughput Identification of Bound Ligands in Protein Crystal Structures
4:30 - 5:00 PM	Coffee Available	Foyer
5:00 - 7:00 PM	Advances in Protein and Nucleic Acid Production and Crystallization	Peak 5
	* Andrzej Joachimiak, Argonne National Laboratory
	Scott A. Lesley, Genomics Institute of the Novartis Research Foundation Applying Structural Genomics Principles to Biology-Centered Targets
	Yaeta Endo, Ehime University Advances in Cell Free Systems for Expression of Eukaryotic and Parasitic Proteins
	Robert T. Batey, University of Colorado, Boulder Advances in Preparation and Crystallization of RNA

	Joseph R. Luft, Hauptman-Woodward Medical Research Institute Short Talk: The Use of Empirically Derived Detergent Phase Boundaries to Crystallize Membrane Proteins
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Breckenridge Ballroom
7:30 - 10:00 PM	Poster Session 1	Breckenridge Ballroom
Sunday, January 10
7:00 - 8:00 AM	Breakfast	Summit Gallery
8:00 - 11:15 AM	Advances in Crystallography, Magnetic Resonance Spectroscopy and Computation	Peak 5
	* Robert M. Stroud, University of California, San Francisco
	Soichi Wakatsuki, KEK IMSS Photon Factory Developments in Synchrotron Protein Crystallography for Targeted Structural Proteomics
	Kurt Wüthrich, ETH Zurich/The Scripps Research Institute NMR in Structural Genomics: New Strategic and Technical Challenges
	John L. Markley, University of Wisconsin, Madison Short Talk: Advances in High-Throughput NMR Spectroscopy of Proteins
	Wayne A. Hendrickson, Columbia University Structural Genomics of Membrane Proteins by X-ray Crystallography
	David Baker, University of Washington Rapid Structure Determination from Sparse Experimental Data Sets using Rosetta
	Michael A. Kennedy, Miami University Short Talk: EPR-Based Long-Range Distance Restraints for Structure Determination of Protein Complexes
9:20 - 9:40 AM	Coffee Break	Foyer
11:15 AM - 1:00 PM	Poster Setup	Breckenridge Ballroom
11:15 AM -	On Own for Lunch and Recreation
1:00 - 10:00 PM	Poster Viewing	Breckenridge Ballroom
2:30 - 4:30 PM	Workshop 2: Structural Genomics and Functional Proteomics of Biological Macromolecules This workshop will focus on novel insights gained from study of the expanding protein universe, protein families, individual organisms, microbiomes, metagenomes, drug targets, pathogens, novel mechanisms, challenging proteins, including RNA, protein-protein complexes, membrane proteins and eukaryotic proteins.	Peak 5
	* Gaetano T. Montelione, Rutgers University
	Marek Grabowski, University of Virginia “Drugability” of Structural Genomics
	Parthasarathy Sampathkumar, Eli Lilly Structures of Two PHR Domains from Mus musculus Phr1 (Mycbp2) Reveal a Novel Fold and Provide Insights into a C. elegans RPM-1 Loss of Function Mutation
	Werner Braun, University of Texas Medical Branch, Allergen Structures in a Genomic Age
	Ashley M. Deacon, Stanford Synchrotron Radiation Laboratory Structural and Functional Studies of the Bacterial Ancestors of Common Eukaryotic Protein Modules
	Liskin Swint-Kruse, University of Kansas Medical School Comparing the Functional Roles of Nonconserved Sequence Positions in Homologous Transcription Repressors: Implications for Sequence/Function Analyses
	András Fiser, Albert Einstein College of Medicine Structural Characteristics of Novel Protein Folds
	Markus Fischer, Columbia University MarkUs: A Server to Navigate Sequence-Structure-Function Space
	Margaret J. Gabanyi, PSI Structural Genomics Knowledgebase - Rutgers University The PSI Structural Genomics Knowledgebase Technology Portal - an Online Resource for all Structural Biologists
	Andrew Ward, The Scripps Research Institute Structural Basis of Drug Binding and Transport in P-glycoprotein
4:30 - 5:00 PM	Coffee Available	Foyer
5:00 - 7:00 PM	Challenging Proteins	Peak 5

	Robert M. Stroud, University of California, San Francisco Ratiocinative Screen of Eukaryotic Integral Membrane Protein Expression and Solubilization for Structure Determination
	Raymond C. Stevens, The Scripps Research Institute Structure-Function of the Human G-Protein Coupled Receptor Family
	Aled M. Edwards, University of Toronto Advances in Structural Proteomics of Human Proteins
	Boguslaw Nocek, Argonne National Laboratory Short Talk: Structure of the HK97-like Bacteriophage DNA Packaging Portal
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Breckenridge Ballroom
7:30 - 10:00 PM	Poster Session 2	Breckenridge Ballroom
Monday, January 11
7:00 - 8:00 AM	Breakfast	Summit Gallery
8:00 - 11:15 AM	Pushing the Limits of Structural Biology II: Advances in Challenging Systems (Joint)	Peaks 1-5
	* Pamela J. Bjorkman, California Institute of Technology

	Steven C. Almo, Albert Einstein College of Medicine Sequence, Structure, Function, Immunity
	Roger W. Hendrix, University of Pittsburgh The Virome and Evolutionary Relationships

	Andrew Ward, The Scripps Research Institute
	Thomas Güttler, Max Planck Institute for Biophysical Chemistry Short Talk: Cargo Recognition by the Nuclear Export Receptor CRM1/Exportin1

	Erik W. Debler, Rockefeller University Short Talk: Characterization of the Nup84 Complex - Paradigm for the Nuclear Pore Complex Structure
	Jamie H.D. Cate, University of California, Berkeley Structures of the Bacterial Ribosome and the Mechanism of Translocation
9:20 - 9:40 AM	Coffee Break	Foyer
11:15 AM - 1:00 PM	Poster Setup	Breckenridge Ballroom
11:15 AM -	On Own for Lunch and Recreation
1:00 - 10:00 PM	Poster Viewing	Breckenridge Ballroom
4:30 - 5:00 PM	Coffee Available	Foyer
5:00 - 6:30 PM	Evolution of Structure and Function	Peak 5
	* Christine A. Orengo, University College London

	Ian A. Wilson, The Scripps Research Institute What have we Learned from Structural Genomics of Proteins of Unknown Function?
	Michael Levitt, Stanford University The Protein Universe: A Current Snapshot
	Barry Honig, Columbia University / HHMI Structural and Functional Relationships between Proteins with Different Global Topologies: Implications for Function Annotation Strategies
6:30 - 7:15 PM	Debate 1: Protein Folds: Fact or Fiction??
	* Ian A. Wilson, The Scripps Research Institute
	Barry Honig, Columbia University / HHMI
	Joel L. Sussman, Weizmann Institute of Science
	Andrej Sali, University of California, San Francisco
	Michael Levitt, Stanford University
	Adam Godzik, Burnham Institute for Medical Research

	David Baker, University of Washington
	Christine A. Orengo, University College London
7:15 - 8:00 PM	Social Hour w/ Lite Bites	Breckenridge Ballroom
7:30 - 10:00 PM	Large-Scale Poster Session: World-Wide Structural Genomics and Structural Proteomics Centers Structural genomic, structural proteomic and protein production centers and consortia highlight the platforms they have developed on new methods, technologies, databases, and computational tools that have spearheaded advances in protein production and structure determination for all classes of targets from bacterial to pathogens to human and on challenging macromolecules, such as membrane proteins, eukaryotic proteins and protein-protein complexes. Emphasis will be placed on what is applicable to the entire community, including single investigator laboratories to increase success and throughput in the study of biological macromolecules. An additional focus of this session will be on contributions of structural genomics to the structure and function of biological macromolecules. Megaposters are encouraged for general overviews from large and medium-sized centers. Normal sized posters are encouraged on specific aspects of structural genomics and structural proteomics, including structural and functional contributions to the study of biological macromolecules.	Breckenridge Ballroom
Tuesday, January 12
7:00 - 8:00 AM	Breakfast	Summit Gallery
8:00 - 11:00 AM	Proteomics and Protein Assemblies/Networks	Peak 5
	* Soichi Wakatsuki, KEK IMSS Photon Factory

	Christine A. Orengo, University College London Identifying Novel Genes Implicated in Function Networks of Biological and Medical Significance
	Adam Godzik, Burnham Institute for Medical Research Structural View of the Gut Microbial Surface Proteome: Implications for Host Immunity and Health
	Michael Washburn, Stowers Institute for Medical Research Quantitative and Probabilistic Assembly of Human Protein Interaction Networks
	Janet Y. Huang, Rutgers University and Northeast Structural Genomics Consortium Short Talk: Human Cancer Protein Interaction Network

	Hannes Braberg, University of California, San Francisco Functional Insights from Protein-Protein and Genetic Interaction Maps
9:20 - 9:40 AM	Coffee Break	Foyer
11:00 AM -	On Own for Lunch and Recreation
4:30 - 5:00 PM	Coffee Available	Foyer
5:00 - 6:30 PM	Exploiting Structure for Function	Peak 5
	* Wayne A. Hendrickson, Columbia University
	Nobutaka Hirokawa, University of Tokyo Kinesin Superfamily Molecular Motors, KIFs and Intracellular Transport: Mechanism of Motility
	Susan S. Taylor, University of California, San Diego Conserved Architecture and Assembly of an Active Protein Kinase
	Peter J. Myler, Seattle Biomedical Research Institute Structural Genomics of Infectious Disease
6:30 - 7:15 PM	Debate 2: Can We Really Determine Function from Structure?
	Steven C. Almo, Albert Einstein College of Medicine
	* Ian A. Wilson, The Scripps Research Institute
	* Stephen K. Burley, Eli Lilly and Company
	Aled M. Edwards, University of Toronto
	Andrzej Joachimiak, Argonne National Laboratory

	Christine A. Orengo, University College London

	Gaetano T. Montelione, Rutgers University
	Susan S. Taylor, University of California, San Diego
7:15 - 8:00 PM	Social Hour w/ Lite Bites	Breckenridge Ballroom
8:00 - 11:00 PM	Entertainment	Breckenridge Ballroom
Wednesday, January 13
	Departure

*Session Chair †Speaker invited, not yet responded.

Keystone Symposia Meeting

Structural Genomics: Expanding the Horizons of Structural Biology (J2)

This meeting took place in the 2010 season.