Keystone Symposia

Biology of B Cells in Health and Disease (B5)

Organizer(s): Mark J. Shlomchik, Cornelius Murre and Timothy W. Behrens
February 6 - 12, 2007
Fairmont Banff Springs · Banff, Alberta
Abstract Deadline: October 6, 2006
Late Abstract Deadline: November 6, 2006
Scholarship Deadline: October 6, 2006
Early Registration Deadline: December 6, 2006

Supported by the Director's Fund

The University of Colorado School of Medicine is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians.

The University of Colorado School of Medicine designates this educational activity for a maximum of 28-35 category 1 credits toward the AMA Physician's Recognition Award. Each physician should claim only those credits that he/she actually spent in the activity.

To receive CME credits, mark the box on the registration form, and pay the additional $50.00.

This meeting took place in the 2007 season.

Listed below are current meetings that are similar to this meeting in nature/content:

B Cells: New Insights into Normal versus Dysregulated Function (D6)
April 12 - 17, 2011

For a complete list of the meetings for the upcoming/current season,
see our meeting list, or search for a meeting.

Summary of Meeting
B lymphocytes secrete antibody, present antigen and regulate immune responses. The study of B cell differentiation has revealed unexpected plasticity. B cells undergo three unique DNA rearrangement processes: V(D)J recombination, somatic hypermutation, and class switch. The latter two occur in the germinal center, where memory B cells as well as long-lived plasma cells are generated. Much research is focused on the signals that generate these cells as well as their intrinsic properties. With regard to normal and aberrant function in disease, B cells frequently undergo transformation, likely via the DNA modification processes unique to B cells. Normally, B cells are tolerant to self but this can break down, leading to autoimmunity. B cells have proven an excellent target for therapy, but the mechanisms remain unclear. The meeting will highlight these interrelated topics by focusing first on molecular events in development and normal immune responses, then covering how these go awry during transformation, autoimmunity and immunodeficiency, with an emphasis on comparing murine and human systems.

Objectives
Upon completion of this conference, participants should be able to:

To demonstrate an understanding of the molecular mechanisms that underlie B cell lineage commitment, subset development and repertoire diversification.
To associate processes of DNA modification unique to B cell development with malignant transformation mechanisms.
To discuss the mechanisms that control lineage and differentiation commitment during B cell immune responses.
To describe how differential fates of Ag-activated B cells have a profound impact on the nature and duration of B cell responses that underlie sustained pathogen resistance after either natural infection or vaccination.

Tuesday, February 6
3:00 - 7:30 PM	Registration	Van Horne Foyer
6:30 - 7:30 PM	Refreshments	Van Horne Foyer
7:30 - 8:30 PM	Keynote Address	Van Horne A-B
	Michael S. Neuberger, Medical Research Council Antibody Diversification through DNA Deamination
Wednesday, February 7
7:00 - 8:00 AM	Breakfast	Alberta, New Brunswick, Cascade, Conservatory, Riverview
8:00 - 11:30 AM	B Development and Gene Expression	Van Horne A-B
	* Cornelis Murre, University of California, San Diego Regulation and Function of Helix- Loop-Helix Proteins in B Cell Development
	Ana Cumano, Pasteur Institute Short Talk: IL-7R Signaling in B Cell Development
	Ranjan Sen, National Institute of Aging, National Institutes of Health Regulation of Immunoglobulin Gene Rearrangements During B Cell Development
	Meinrad Busslinger, Research Institute of Molecular Pathology Transcriptional Control of B Cell Development
	Harinder Singh, University of Chicago Specification of the B Cell Fate and the Regulation of Immunoglobulin Gene Recombination
	David M. Allman, University of Pennsylvania Short Talk: Generation of Peripheral B cells Occurs via Two Spatially and Temporally Distinct Pathways
	Shiv Pillai, Massachusetts General Hospital Short Talk: A Cell Surface Acetylation/Deacetylation Paradigm for the Regulation of Antigen Receptor Signaling, B Lymphocyte Development, and Humoral Autoimmunity
9:30 - 9:50 AM	Coffee Break	Van Horne Foyer
11:30 AM - 1:00 PM	Poster Setup	Alberta, New Brunswick, Cascade, Conservatory, Riverview
1:00 - 10:00 PM	Poster Viewing	Alberta, New Brunswick, Cascade, Conservatory, Riverview
4:30 - 5:00 PM	Coffee Available	Van Horne Foyer
5:00 - 7:15 PM	B Cell Signaling and Activation	Van Horne A-B
	* Alexander (Sasha) Tarakhovsky, Rockefeller University Role of Argonaute 2 in B Cell Function
	Michael Reth, Max Planck Institute for Immunobiology Early Events during Signal Transduction from the B Cell Antigen Receptor
	Klaus Rajewsky, PCMM, Children's Hospital Boston, Immune Disease Institute Aspects of Tanscriptional and microRNA Cntrol in B cell Development
	Edward A. Clark, University of Washington Medical Center Short Talk: Regulation of B Cell Entry into Cell Cycle by Superoxide and CD22
	David J. Rawlings, University of Washington / Seattle Children's Hospital Research Institute Short Talk: Molecular Mechanism(s) Controlling PKC-Dependent, Immunoreceptor-Induced Signals
7:15 - 8:15 PM	Social Hour w/ Lite Bites	Alberta, New Brunswick, Cascade, Conservatory, Riverview
7:30 - 10:00 PM	Poster Session 1	Alberta, New Brunswick, Cascade, Conservatory, Riverview
Thursday, February 8
7:00 - 8:00 AM	Breakfast	Alberta, New Brunswick, Cascade, Conservatory, Riverview
8:00 - 11:30 AM	SHM/CSR/VDJ	Van Horne A-B
	David G. Schatz, Yale University School of Medicine Targeting of Somatic Hypermutation to the Immunoglobulin Locus
	Mark S. Schlissel, University of California, Berkeley Regulating V(D)J Recombination In Vivo
	Yehudit Bergman, Hebrew University, Hadassah Medical School Epigenetic ontogeny of the Ig light chain locus during B cell development
	* Frederick W. Alt, HHMI, Children's Hospital and Immune Disease Institute, Boston Joining Pathways that Mediate IgH Class Switch Recombination and Suppress Oncogenic Translocations
	Patricia J. Gearhart, National Institute of Aging, National Institutes of Health Short Talk: Deoxyuracils in Immunoglobulin Genes from Germinal Center B Cells
	Janet M. Stavnezer, University of Massachusetts Medical School Short Talk: DNA Breaks and DNA Repair during Antibody Class Switching
	Amy L. Kenter, University of Illinois College of Medicine Short Talk: Long Range Interactions between Germline Transcript Regulatory Elements Drives S/S Synapsis during Class Switch Recombination
9:30 - 9:50 AM	Coffee Break	Van Horne Foyer
11:30 AM - 1:00 PM	Poster Setup	Alberta, New Brunswick, Cascade, Conservatory, Riverview
1:00 - 10:00 PM	Poster Viewing	Alberta, New Brunswick, Cascade, Conservatory, Riverview
2:30 - 4:30 PM	Workshop 1A: Development and Function of B Cell Subsets	Theatre
	* Shiv Pillai, Massachusetts General Hospital
	* David M. Allman, University of Pennsylvania
	Alaitz Aranburu, Research Centre, Ospedale Bambino Gesu B Cells of Natural Mucosal Immunity Derive from Splenic Precursors
	Hans-Willem E. Snoeck, Mount Sinai School of Medicine Evidence for Two Distinct B-Cell Differentiation Routes
	Dan Northrup, University of Pennsylvania Resolution of B1 B Cells in Adult Bone Marrow
	Natalia V. Giltiay, Cleveland Clinic Foundation Act1 Regulates the Survival and Maturation of Transitional B-cells
	Cynthia J. Guidos, University of Toronto and Hospital for Sick Children Lunatic and Manic Fringe Enhance Notch2/Delta-like-1 Interactions during Marginal Zone B Cell Development in Splenic Niches

	Robert H. Carter, NIAMS, National Institutes of Health The Role of CD19 in the Development of Marginal Zone
	Frederic Sierro, Garvan Institute of Medical Research TRAF2-deficient MZ B Cells Differentiate and Survive in the Absence of BAFF
2:30 - 4:30 PM	Workshop 1B: SHM/CSR/VDJ	Van Horne A/B
	* Amy L. Kenter, University of Illinois College of Medicine
	* Ann J. Feeney, The Scripps Research Institute Deciphering the Histone Code Controlling Immunoglobulin Gene Rearrangement
	Adam G. W. Matthews, Massachusetts Institute of Technology RAG2 PHD Finger Couples Histone H3 Lysine 4 Trimethylation with V(D)J Recombination
	Kevin M. McBride, Rockefeller University Regulation of CSR and Hypermutation by Multiple AID Phosphorylation Sites
	Catherine T. Yan, CBR Institute for Biomedical Research DNA Polymerase eta is the Sole Contributor of A-T Modifications during Immunoglobulin Gene Hypermutation in the Mouse
	Jean-Claude Weill, INSERM DNA Polymerase eta is the Sole Contributor of A-T Modifications during Immunoglobulin Gene Hypermutation in the Mouse
	Heinz Jacobs, Netherlands Cancer Institute Somatic Hypermutation in B Cells Lacking PCNA K164 Modifications
2:30 - 4:30 PM	Workshop 1C: Computation and Mathematical Modeling in the Study of B Cell Biology, Supported by the Society for Mathematical Biology	Shaughnessey
	* Uri Hershberg, Yale University School of Medicine Introductory Remarks - Different View of B Cell Immunology
	Michal Or-Guil, Institute for Theoretical Biology Continuous Renewal of Germinal Center Populations: The True Strategy of Affinity Maturation?
	* Steven H. Kleinstein, Yale University School of Medicine The Impact of Affinity on B Cell Proliferation and Death
	Phil D. Hodgkin, Walter and Eliza Hall Institute of Medical Research The Cyton Model of B Lymphocyte Growth and Regulation
	Yoram Louzoun, Bar Ilan University Protein-Lipid Interactions Mediate B Cell Activation: in-silico Cell Surface Modeling
	Roger Sciammas, University of Chicago Dynamics of the Gene Regulatory Network Orchestrating Isotype Switching and Plasma Cell Differentiation
	Ramit Mehr, Bar-Ilan University Antigen-Driven Selection in Germinal Centers as Reflected by Shape Characteristics of Immunoglobulin Gene Lineage Trees: A Large-Scale Simulation Study
	Edward J. Steele, Australian National University A-to-G Spectrum Correlates with RNA Hairpins at WA Hotspots: A Role for A-to-I RNA Editing in A-T Targeted Phase II SHM
4:30 - 5:00 PM	Coffee Available	Van Horne Foyer
5:00 - 7:15 PM	B Cell Immune Responses	Van Horne A-B
	* Jason G. Cyster, University of California, San Francisco Cellular Migration during the B Cell Immune Response
	Frances E. Lund, University of Rochester Medical Center Regulation of Type 2 Immune Responses by Cytokine-Producing and Antigen-Presenting B cells
	Elizabeth A. Leadbetter, Brigham and Women's Hospital Short Talk: CD1d-Restricted iNK T Cell Help for Lipid Antigen Specific Antibodies

	Ann M. Haberman, Yale University School of Medicine Short Talk: Visualization of Germinal Center (GC) B Cell Dynamics by Multi-Photon Microscopy
	Peter Bergqvist, University of Gothenburg Short Talk: Gut IgA Class Switch Recombination in the Absence of CD40 does not Occur in the Lamina Propria and is Independent of Germinal Centers
7:15 - 8:15 PM	Social Hour w/ Lite Bites	Alberta, New Brunswick, Cascade, Conservatory, Riverview
7:30 - 10:00 PM	Poster Session 2	Alberta, New Brunswick, Cascade, Conservatory, Riverview
Friday, February 9
7:00 - 8:00 AM	Breakfast	Alberta, New Brunswick, Cascade, Conservatory, Riverview
8:00 - 11:00 AM	Memory B Cells/Plasma Cells	Van Horne A-B
	* Mark J. Shlomchik, Yale University School of Medicine Intrinsic Properties of Memory B Cells



	Toshitada Takemori, RCAI, Riken Pathways of Memory B Cell Development
	Ulf Klein, Columbia University Transcriptional Control of Plasma Cell Differentiation
	David M. Tarlinton, Walter and Eliza Hall Institute of Medical Research Short Talk: Plasma Cell Differentiation
	Kenneth G.C. Smith, University of Cambridge Short Talk: FcgammaRIIB Controls Bone Marrow Plasma Cell Persistence and Mediates Apoptosis: Implications for Plasma Cell Homeostasis, Autoimmunity and Myeloma
	Rachel C. Ettinger, NIAMS, National Institutes of Health Short Talk: Essential Role of IL-21 for Plasma Cell Generation During T Cell- B Cell or T Cell- DC- B Cell Collaboration
9:30 - 9:50 AM	Coffee Break	Van Horne Foyer
11:30 AM - 1:00 PM	Poster Setup	Alberta, New Brunswick, Cascade, Conservatory, Riverview
1:00 - 10:00 PM	Poster Viewing	Alberta, New Brunswick, Cascade, Conservatory, Riverview
2:30 - 4:30 PM	Workshop 2A: Transcription Factors in B Cell Development and Differentiation	Van Horne A/B
	* Jane A. Skok, New York University School of Medicine Pericentromeric Recruitment and Decontraction of the IgH Locus Depend on Different Signaling Requirements
	* Patrick Matthias, Friedrich Miescher Institute for Biomed Research

	Timothy P. Bender, University of Virginia Health System c�Myb is Critical for Early B Cell Development and Maintenance of Follicular B Cells

	Hart S. Dengler, Burnham Institute A Non-redundant Role for FoxO1 in Early B Cell Differentiation
	Lee Ann Garrett-Sinha, University at Buffalo, State University of New York Ets-1 Regulates Plasmacytic Differentiation by Interfering with the Activity of the Transcription Factor Blimp-1
	Arthur L. Shaffer, National Cancer Institute, National Institutes of Health An shRNA �Achilles� heel� Screen Reveals the Requirement for IRF4 Expression in Plasma Cells and Multiple Myleoma
	Roger Sciammas, University of Chicago Graded Expression of Irf4 Coordinates Isotype Switching with Plasma Cell Differentiation
2:30 - 4:30 PM	Workshop 2B: Germinal Centers, Plasma Cells, and Memory Cells	Shaughnessey
	* David M. Tarlinton, Walter and Eliza Hall Institute of Medical Research
	* Stuart G. Tangye, Garvan Institute of Medical Research Interplay between IL-21 and IL-4 in Regulating isotype Switching, Ig Secretion and Differentiation of Human B-Cell Subsets
	Jenni E. Crowley, University of Pennsylvania Primary and Memory B Cells Occupy Independent Homeostatic Niches

	Tingting Zhang, University of Manitoba Role of Bam32 in T-Cell Dependent Antibody Responses
	Tanja Anke Schwickert, Rockefeller University Germinal Center Imaging Reveals a Dynamic Open Structure
	Isharat Yusuf, MedImmune Fyn is Required for Optimal Germinal Center Formation and Memory B Cell Generation
	Shiang-Jong Tzeng, National Taiwan University Fc�RIIB1 Regulates Plasma Cell Apoptosis and Differentiation through a c-Abl-dependent Pathway
	Robert Brink, Garvan Institute of Medical Research Antigen Affinity Regulates Plasma Cell Differentiation
4:30 - 5:00 PM	Coffee Available	Van Horne Foyer
5:00 - 7:30 PM	B Cell Tolerance	Van Horne A-B
	Martin G. Weigert, University of Chicago Anti-DNA Antibodies and Autoimmunity
	David Nemazee, The Scripps Research Institute RS Knockout Mouse: A Mutant with a Targeted Defect in Receptor Editing

	Chandra Mohan, University of Texas Southwestern Medical Center Lupus Susceptibility Loci at the Interface of Tolerance and Autoimmunity
	* Michel C. Nussenzweig, Rockefeller University Development of Autoantibodies in Normal Humans and in Patients with SLE/GC Imaging Reveals a Dynamic Open Structure
	Marcus R. Clark, University Of Chicago Short Talk: Regulation of B Cell Antigen Receptor Endocytic Trafficking in Anergy
7:30 - 8:30 PM	Social Hour w/ Lite Bites	Alberta, New Brunswick, Cascade, Conservatory, Riverview
7:30 - 10:00 PM	Poster Session 3	Alberta, New Brunswick, Cascade, Conservatory, Riverview
Saturday, February 10
7:00 - 8:00 AM	Breakfast	Alberta, New Brunswick, Cascade, Conservatory, Riverview
8:00 - 11:00 AM	B Cell Transformation	Van Horne A-B
	Andr� Nussenzweig, National Institutes of Health DNA Damage and Oncogenic Stress: Barriers to Chromosomal Translocations
	Riccardo Dalla-Favera, Columbia University Molecular Pathogenesis of B Cell Lymphomas
	Nicholas Chiorazzi, Feinstein Institute for Medical Research Short Talk: The Biology of Chronic Lymphocytic Leukemia B Cells
	Robert C. Rickert, Burnham Institute for Medical Research Short Talk: Coordinate Suppression of B Cell Lymphoma Progression by SHIP and PTEN
	Jayne S. Danska, Hospital for Sick Children Research Institute Short Talk: Subversion of Pre-B Cell Receptor Signaling during Progression to Pre-B Cell Leukemia in p53-/-Ptprcscid/scid mice

	* Louis M. Staudt, NCI, National Institutes of Health Loss of Function RNA Interference Screens for Molecular Targets in Lymphoid Malignancies
9:30 - 9:50 AM	Coffee Break	Van Horne Foyer
11:00 AM - 1:00 PM	Poster Setup	Alberta, New Brunswick, Cascade, Conservatory, Riverview
1:00 - 10:00 PM	Poster Viewing	Alberta, New Brunswick, Cascade, Conservatory, Riverview
2:30 - 4:30 PM	Workshop 3A: Autoreactivity and Tolerance	Van Horne A/B
	* Qiang Pan-Hammarstr�m, Karolinska Institute
	* Nina Luning Prak, University of Pennsylvania
	Anupama Ahuja, Yale University School of Medicine Amelioration of Disease in Lupus Prone MRL/lpr Mice by Depletion of B Cells
	Christopher G. Mayne, University of Wisconsin Systemic Autoimmunity in BAFF-R Mutant A/WySnJ Mice
	Stefanie Sarantopoulos, Dana Farber Cancer Institute High BAFF/Blys Results in Increased Plasmablasts in Human Chronic Graft Versus Host Disease

	Laura B. Ramsey, University of Minnesota Basal B Cell Receptor Signaling and Receptor Editing
	Anil K. Panigrahi, University of Pennsylvania School of Medicine RS Recombination as a Potential Marker of Receptor Editing and Autoreactivity
	Nathan R. Schoettler, University of Chicago V Gene Repertoires and Editing in Human Autoimmunity
2:30 - 4:30 PM	Workshop 3B: B Cell Malignancy	Shaughnessey
	* Jayne S. Danska, Hospital for Sick Children Research Institute
	* Cynthia J. Guidos, University of Toronto and Hospital for Sick Children
	Gerald V. Denis, Boston University School of Medicine Tumor-specific and Proliferation-specific Gene Expression Typifies Murine Transgenic (Tg) B Cell Lymphomagenesis
	John P. Manis, Harvard Medical School 53BP1 is Targeted to Sites of DNA Breaks within the Immunoglobulin Heavy Chain Locus to Promote Class Switch Recombination
	Maggie M. Liu, Yale University The Mutation Load Suffered by the Mouse Genome as a Consequence of Somatic Hypermutation
	Van B. T. Ta, Erasmus Medical Center Role of V(D)J Recombination in SLP-65 Deficient Pre-B Cell Leukemia Formation
	Laurence Lamy, National Cancer Institute, National Institutes of Health A Loss of Function RNA Interference Screen Identifies Genes Involve in Multiple Myeloma Proliferation and Survival
	Jing Wang, Immune Disease Institute Aberrant Class Switch Recombination and Secondary V(D)J Recombination Lead to Oncogenic Translocations and B Cell Lymphoma
4:30 - 5:00 PM	Coffee Available	Van Horne Foyer
5:00 - 7:15 PM	Autoimmunity and Immunodeficiency	Van Horne A-B
	* Ann Marshak-Rothstein, University of Massachusetts Medical School Toll-like Receptor Signaling in Systemic Autoimmune Disease
	Stephen M. Anderton, University of Edinburgh B Cells and the Regulation of CNS Autoimmune Disease
	Garry P. Nolan, Stanford University Single Cell Kinase Signaling for Studies of Disordered Autoimmune Signaling in Systemic Lupus Erythematosus
	Jonathan A. Deane, NIAID, National Institutes of Health Short Talk: TLR7 Gene Dosage is Critical for Autoantibody Production and Pathology in Mouse Models of Lupus
	Fabienne Mackay, Nursing and Health Sciences Central Clinical School Short Talk: The Role of BAFF in Autoimmunity is T Cell-Independent but Requires Toll-Like Receptor (TLR) Signalling
7:15 - 8:15 PM	Social Hour w/ Lite Bites	Alberta, New Brunswick, Cascade, Conservatory, Riverview
7:30 - 10:00 PM	Poster Session 4	Alberta, New Brunswick, Cascade, Conservatory, Riverview
Sunday, February 11
7:00 - 8:00 AM	Breakfast	Alberta, New Brunswick, Cascade, Conservatory, Riverview
8:00 - 11:15 AM	Autoimmunity and Immunodeficiency in Human	Van Horne A-B
	* Timothy W. Behrens, Genentech, Inc. The Genetics and Molecular Defects Associated with SLE
	Flavius Martin, Genentech, Inc. Efficacy and Mechanism of B Cell Depletion in Treatment of Autoimmune Disorders in Humans

	Anne Durandy, H�pital Necker-Enfants Malade Immunoglobulin Class Switch Recombination Deficiency Associated to a DNA Repair Defect
	Bodo Grimbacher, Royal Free Hospital & University College Medical School Antibody Deficiencies: The Role of Costimulation
	I�aki E. Sanz, University of Rochester Short Talk: Breakdown and restoration of B cell tolerance in human SLE
	Eric Meffre, Yale University School of Medicine Short Talk: Defective and Alternative B Cell Tolerance Mechanisms in Rheumatoid Arthritis
9:30 - 9:50 AM	Coffee Break	Van Horne Foyer
4:30 - 5:00 PM	Coffee Available	Van Horne Foyer
5:00 - 7:00 PM	B Cells in Pathogen Resistance	Van Horne A-B
	* Rolf M. Zinkernagel, University Hospital Zurich Antiviral Antibody Responses

	Mathias Florian Heikenw�lder, University Hospital Z�rich The Role of B Lymphocytes in Peripheral Prion Pathogenesis
	John F. Kearney, University of Alabama at Birmingham Marginal Zone B-lymphocytes: Development and Function.

	Troy D. Randall, University of Rochester Medical Center Short Talk: Role of B Cells in Heterosubtypic Immunity to Influenza virus
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Alberta, New Brunswick, Cascade, Conservatory, Riverview
8:00 - 11:00 PM	Entertainment	Alberta, New Brunswick, Cascade, Conservatory, Riverview
Monday, February 12
	Departure

*Session Chair †Speaker invited, not yet responded.

Keystone Symposia Meeting

Biology of B Cells in Health and Disease (B5)

This meeting took place in the 2007 season.