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Molecular Basis for Chromatin Structure and Regulation (A8)

Organizer(s): Jonathan Widom, Geeta Narlikar and Dinshaw J. Patel
January 17 - 22, 2010
Sagebrush Inn and Conference Center  ·  Taos, New Mexico
Abstract Deadline: September 18, 2009
Late Abstract Deadline: October 21, 2009
Scholarship Deadline: September 18, 2009
Early Registration Deadline: November 17, 2009


Supported by The Directors' Fund



This meeting took place in the 2010 season.

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Summary of Meeting
Chromatin structure and dynamics underlie every aspect of genome function. The past several years have seen an exponential growth in our knowledge of facts, ranging from catalogs of histone protein posttranslational modifications to complete genome sequences of many organisms to atomic resolution structures of many key molecular players. But our mechanistic understanding lags far behind. New reagents, methodologies and concepts are needed. This meeting will bring together leading experts in diverse areas of chromatin biology who are developing and applying new approaches to develop a concrete mechanistic link between chromatin structure and chromatin function. The topics covered include: fundamental in vivo physical chemical studies of transcription factor binding; real-time single molecule in vivo imaging; state-of-the-art methods for discovery, characterization, and manipulation of histone- and nucleosome-modifying enzymes and for the synthesis and characterization of their specifically-modified histone and nucleosome substrates; single molecule biophysics studies of molecular mechanisms; atomic resolution structural studies; and locus- and chromosome-wide phenomena such as heterochromatin formation and dosage compensation.

Sunday, January 17
3:00 - 7:30 PM Registration Chamisa Lobby
6:30 - 7:30 PM Refreshments Chamisa Lobby
7:30 - 9:30 PM Keynote Session Chamisa Ballroom 1
* Jonathan Widom, Northwestern University
Roger D. Kornberg, Stanford University
Chromatin Structure and Transcription
C. David Allis, Rockefeller University
Beyond the Double Helix: Writing and Reading the Histone Code
Monday, January 18
7:00 - 8:00 AM Breakfast Los Vaqueros
8:00 - 11:15 AM Transcription Factor and Chromatin Dynamics in vivo Chamisa Ballroom 1
Timothy J. Stasevich, National Institutes of Health
Dissecting the Binding Mechanism of the Linker Histone in Live Cells: An Integrated FRAP Analysis
John T. Lis, Cornell University
Very Rapid and Locus-Wide Changes in Chromatin Caused by Activation of a Potentiated Gene
X. Sunney Xie, Harvard University
Transcription Factor and Chromatin Dynamics in vivo
Susan M. Gasser, Friedrich Miescher Institute for Biomedical Research
The Role of Nucleosome Remodelers in the Positioning and Dynamics of DNA during Expression and Repair
Hinrich Boeger, University of California, Santa Cruz
Short Talk: The Kinetics of Stochastic Fluctuations in Chromatin Structure and Gene Expression
Maya M. Kasowski, Yale University
Short Talk: Analyzing Variation in Gene Regulation in Humans: Global Analysis of NF-kappaB and Polii Binding using CHIP-SEQ
Alexander (Sasha) Tarakhovsky, Rockefeller University
Short Talk: Histone Mimicry by Pathogens
9:20 - 9:40 AM Coffee Break Chamisa Lobby
11:15 AM - On Own for Lunch and Recreation
11:15 AM - 1:00 PM Poster Setup Chamisa Ballroom 2
1:00 - 10:00 PM Poster Viewing Chamisa Ballroom 2
3:30 - 4:30 PM Workshop Panel
To be geared toward helping women (and men, if interested) graduate students, postdocs, and junior faculty address the challenges of balancing an academic career and family.
Chamisa Ballroom 1
* Geeta J. Narlikar, University of California, San Francisco
4:30 - 5:00 PM Coffee Available Chamisa Lobby
5:00 - 7:00 PM Functional Elements Chamisa Ballroom 1
* Gary Felsenfeld, National Institutes of Health
Insulators and Enhancer Blockers
Stavros Lomvardas, University of California, San Francisco
Molecular Mechanisms of Olfactory Receptor Choice
Robert E. Kingston, Massachusetts General Hospital
Nucleosome Occupancy and Transcriptional Regulation
JJ L. Miranda, University of California, San Francisco
Short Talk: Molecular Architecture of CTCF and the Insulatosome
7:00 - 8:00 PM Social Hour w/ Lite Bites Chamisa Ballroom 2
7:30 - 10:00 PM Poster Session 1 Chamisa Ballroom 2
Tuesday, January 19
7:00 - 8:00 AM Breakfast Los Vaqueros
8:00 - 11:15 AM Chromatin Assembly and Modification Chamisa Ballroom 1
* Karolin Luger, Colorado State University
Histone Chaperone Mechanisms in Chromatin Assembly and Disassembly
Geneviève Almouzni, Centre National de la Recherche Scientifique
Histone Chaperones, Chromatin Assembly and the Challenges of Cell Cycle
James T. Kadonaga, University of California, San Diego
Chromatin Assembly and DNA Dynamics
Shelley L. Berger, University of Pennsylvania
Histone Covalent Modifications in Genome Regulation
Song Tan, Pennsylvania State University
Short Talk: Structural Basis for Nucleosome Recognition by the RCC1 Chromatin Factor
Michael A. Shogren-Knaak, Iowa State University
Short Talk: Acetylation-Dependent Multimerization of the Yeast SAGA Coactivator Complex in Inducible Gene Transcription
9:20 - 9:40 AM Coffee Break Chamisa Lobby
11:15 AM - On Own for Lunch and Recreation
11:15 AM - 1:00 PM Poster Setup Chamisa Ballroom 2
1:00 - 10:00 PM Poster Viewing Chamisa Ballroom 2
4:30 - 5:00 PM Coffee Available Chamisa Lobby
5:00 - 7:00 PM Histone Code Modules and Enzyme Mechanism Chamisa Ballroom 1
* Dinshaw J. Patel, Memorial Sloan-Kettering Cancer Center
Or P. Gozani, Stanford University
Role of Protein Lysine Methylation Signaling in Health and Disease
Cheryl Arrowsmith, University of Toronto
Structural and Chemical Biology of Histone Code Modules
Philip A. Cole, Johns Hopkins University School of Medicine
Chemical Approaches to Mechanisms of Histone Modifying Enzymes
Alexander J. Ruthenburg, University of Chicago
Short Talk: Multivalent Interactions Interpret the Histone Code at the Nucleosome Level
7:00 - 8:00 PM Social Hour w/ Lite Bites Chamisa Ballroom 2
7:30 - 10:00 PM Poster Session 2 Chamisa Ballroom 2
Wednesday, January 20
7:00 - 8:00 AM Breakfast Los Vaqueros
8:00 - 11:15 AM Dynamic Transitions and ATP-Dependent Remodeling Chamisa Ballroom 1
* Bradley R. Cairns, Huntsman Cancer Institute, University of Utah
DNA Translocation by the RSC Chromatin Remodeler in ‘Tethered’ Formats
Geeta J. Narlikar, University of California, San Francisco
Structure and Mechanisms of ATP-Dependent Chromatin Remodeling Motors
Michelle D. Wang, Cornell University
The Dynamics of Accessing DNA
Xiaowei Zhuang, Harvard University
Single Molecule Studies of Chromatin Remodeling Enzymes
Gregory D. Bowman, Johns Hopkins University
Short Talk: Crystal Structure of the Chd1 Remodeler Reveals that Chromodomains can Directly Regulate the ATPase Motor
Hua-Ying Fan, Fox Chase Cancer Center
Short Talk: UV-Induced Association of the CSB Remodeling Protein with Chromatin Requires ATP-Dependent Relief of N-Terminal Autorepression
9:20 - 9:40 AM Coffee Break Chamisa Lobby
11:15 AM - On Own for Lunch and Recreation
11:15 AM - 1:00 PM Poster Setup Chamisa Ballroom 2
1:00 - 10:00 PM Poster Viewing Chamisa Ballroom 2
4:30 - 5:00 PM Coffee Available Chamisa Lobby
5:00 - 7:00 PM Chromatin Structure Chamisa Ballroom 1
* Geeta J. Narlikar, University of California, San Francisco
Timothy J. Richmond, ETH Zürich
Structure and Interactions of the Chromatin Remodeling Factor ISW1a
Eran Segal, Weizmann Institute of Science
EMBO Young Investigator Lecture: Toward Understanding the Code of Chromatin Regulation
John van Noort, BF/ LION, Leiden University
Unraveling Chromatin Higher Order Structure with Magnetic Tweezers
Yvonne N. Fondufe-Mittendorf, Northwestern University
Short Talk: Nucleosome Positioning and Dynamic Nucleosome Repositioning in Transcription Regulation
7:00 - 8:00 PM Social Hour w/ Lite Bites Chamisa Ballroom 2
7:30 - 10:00 PM Poster Session 3 Chamisa Ballroom 2
Thursday, January 21
7:00 - 8:00 AM Breakfast Los Vaqueros
8:00 - 11:15 AM Silencing and Dosage Compensation Chamisa Ballroom 1
* Danesh Moazed, Harvard Medical School
In Vitro Reconstitution of Heterochromatin-Dependent Transcriptional Gene Silencing
Shiv I. S. Grewal, NCI, National Institutes of Health
RNAi-Mediated Epigenetic Control of the Genome
Barbara J. Meyer, University of California, Berkeley
X-Chromosome-Wide Gene Regulation
Jeannie T. Lee, Massachusetts General Hospital
Separating Repressive and Activating Chromatin Domains at the X-Inactivation Center
Joanna Wysocka, Stanford School of Medicine
Short Talk: Coordinated Control of PRC2 Enzymatic Activity and Target Gene Occupancy in Pluripotent Cells
Yali Dou, University of Michigan
Short Talk: The Heterodimer of Non-SET Domain Proteins Ash2L and RbBP5 has Intrinsic Histone Methyltransferase Activity that Synergizes with the MLL1 SET Domain in H3 K4 Methylation
9:20 - 9:40 AM Coffee Break Chamisa Lobby
11:15 AM - On Own for Lunch and Recreation
4:30 - 5:00 PM Coffee Available Chamisa Lobby
5:00 - 7:00 PM Protein Chemistry Chamisa Ballroom 1
* Tom W. Muir, Rockefeller University
A Synthetic ‘Kiss of Death’
Dinshaw J. Patel, Memorial Sloan-Kettering Cancer Center
A Structural Perspective of How Chromatin-Binding Modules Interpret Histone Modifications
Jennifer J. Ottesen, Ohio State University
Short Talk: Ligation Strategies to Probe Histone Modifications in the Nucleosome Core
Benjamin A. Garcia, Princeton University
Short Talk: Next Generation Quantitative Proteomic Tools for Analyzing Histone Modifications
Daniele Canzio, University of California, San Francisco
Short Talk: Polymerizable Chromodomain "Sticky Ends" in HP1 Couple H3K9me3 Recognition to Nucleosome Arrangement in Heterochromatin Assembly
7:00 - 8:00 PM Social Hour w/ Lite Bites Chamisa Ballroom 2
8:00 - 11:00 PM Entertainment Chamisa Ballroom 2
Friday, January 22
Departure
*Session Chair   †Speaker invited, not yet responded.



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