Keystone Symposia | Scientific Conferences on Biomedical and Life Science Topics

RNA Silencing Mechanisms in Plants (B6)

Organizer(s): Marjori Ann Matzke and James C. Carrington
February 21 - 26, 2010
Hilton Santa Fe/Historic Plaza · Santa Fe, New Mexico
Abstract Deadline: October 21, 2009
Late Abstract Deadline: November 20, 2009
Scholarship Deadline: October 21, 2009
Early Registration Deadline: December 21, 2009

Sponsored by Gordon and Betty Moore Foundation and Monsanto Company

This meeting took place in the 2010 season.

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see our meeting list, or search for a meeting.

Summary of Meeting
Although the core mechanisms of RNA silencing are conserved across eukaryotes, plants have deployed gene silencing in a particularly wide variety of molecular, developmental, adaptive, defensive and epigenetic contexts. Plants, therefore, serve as among the most insightful models to illuminate mechanisms of RNA-directed DNA methylation and transcriptional silencing, antiviral defense, small RNA biogenesis and effector mechanisms, and the roles of small RNA-mediated regulation in development and stress responses. We will highlight recent mechanistic advances about how RNA silencing pathways integrate with plant processes, acting at levels ranging from single-nucleotide to population, as regulatory devices. Session topics emphasize integration between silencing mechanisms and developmental regulation and stress responses, DNA and chromatin modification, host-pathogen dynamics, and natural variation. The Symposium will also highlight cutting-edge, high-throughput technology being developed and applied to understand the roles of RNA-mediated silencing across the plant genome.

Sunday, February 21
3:00 - 7:30 PM	Registration	Promenade
6:30 - 7:30 PM	Refreshments	Promenade
7:30 - 8:30 PM	Keynote Address	Mesa Ballroom
	* Marjori Ann Matzke, Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences
	David C. Baulcombe, University of Cambridge Small RNAs in Plants
8:30 - 8:45 PM	Orientation for New Attendees and New Investigators Keystone Symposia's Chief Scientific Officer, Dr. Andy Robertson, to lead discussion in a "What to Expect during your Attendance" for interested delegates.	Mesa Ballroom
Monday, February 22
7:00 - 8:00 AM	Breakfast	Chamisa/Ortiz
8:00 - 11:15 AM	Small RNA Biogenesis and Effector Mechanisms	Mesa Ballroom
	* Marja Timmermans, Cold Spring Harbor Laboratory
	James C. Carrington, Oregon State University Small RNA-Directed Silencing Pathways in Plants
	Herve Vaucheret, Institut National de la Recherche Agronomique Diversity of and Crosstalk between RNA Degradation Pathways
	Yijun Qi, National Institute of Biological Sciences Plant MicroRNA Effector Complexes and Targets
	Nina V. Fedoroff, Pennsylvania State University MicroRNA Precursor Processing in Arabidopsis
	Javier F. Palatnik, Institute of Molecular and Cell Biology of Rosario Short Talk: Mechanisms of microRNA Processing in Plants
	Kumakura Naoyoshi, University of Tokyo Short Talk: Analysis of SGS3/RDR6-Body Related to Biogenesis of ta-siRNAs and vsiRNAs
	Zhizhong Gong, China Agricultural University Short Talk: Transcriptional Gene Silencing Independent on siRNA-Directed DNA Methylation in Arabidopsis: Characterization of ros1 Suppressors and Identification of more ROS Genes
9:20 - 9:40 AM	Coffee Break	Promenade
11:15 AM -	On Own for Lunch and Recreation
11:15 AM - 1:00 PM	Poster Setup	Mesa C/Chamisa
1:00 - 10:00 PM	Poster Viewing	Mesa C/Chamisa
4:30 - 5:00 PM	Coffee Available	Promenade
5:00 - 7:00 PM	Silencing and Intercellular Signals	Mesa Ballroom
	* R. Scott Poethig, University of Pennsylvania
	Marja Timmermans, Cold Spring Harbor Laboratory Pattern Formation by Mobile Small RNAs
	Olivier Voinnet, Institut de Biologie Moléculaire des Plantes Cell to Cell Movement of RNA Silencing Signals
	Julia Kehr, Centre of Plant Biotechnology and Genomics The Roles of Small Phloem RNAs in Stress Responses
	Nial R. Gursanscky, University of Queensland Short Talk: Genetic Determinants Involved in Transmission of Gene Silencing from Rootstocks of Arabidopsis
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Mesa Ballroom
7:30 - 10:00 PM	Poster Session 1	Mesa C/Chamisa
Tuesday, February 23
7:00 - 8:00 AM	Breakfast	Chamisa/Ortiz
8:00 - 11:15 AM	Epigenetics, Heterochromatin and DNA Methylation	Mesa Ballroom
	* David C. Baulcombe, University of Cambridge
	Steven E. Jacobsen, HHMI/University of California, Los Angeles DNA Methylation in Arabidopsis

	Jay B. Hollick, University of California, Berkeley Co-Option of Pol IV for Gene Regulation in Maize
	Vincent Colot, Ecole Normale Supérieure RNAi-Dependent and Independent DNA Methylation of Repeat Elements and Epigenetic Control of Genes in Arabidopsis
	Jerzy Paszkowski, University of Geneva DNA Methylation and Transgenerational Epigenetic Inheritance
	Soichi Inagaki, National Institute of Genetics Short Talk: Role of a Jumonji Domain Protein IBM1 in Differential DNA Methylation and Histone Modification between Genes and Transposons in Arabidopsis Thaliana

	Janne Lempe, University of Washington Short Talk: PolV as a Potential Capacitor of Phenotypic Variance in Plants
9:20 - 9:40 AM	Coffee Break	Promenade
11:15 AM -	On Own for Lunch and Recreation
11:15 AM - 1:00 PM	Poster Setup	Mesa C/Chamisa
1:00 - 10:00 PM	Poster Viewing	Mesa C/Chamisa
4:30 - 5:00 PM	Coffee Available	Promenade
5:00 - 7:00 PM	Integration of Silencing Mechanisms, Development and Stress Responses	Mesa Ballroom
	* Javier F. Palatnik, Institute of Molecular and Cell Biology of Rosario
	* Marjori Ann Matzke, Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences
	Sarah C. Hake, Plant Gene Expression Center MicroRNA Mediated Regulation of Organogenesis in Maize
	Jian-Kang Zhu, University of California, Riverside Small RNAs Related to Stress and DNA Demethylation
	R. Scott Poethig, University of Pennsylvania miRNAs and Developmental Timing in Plants
	Alexis M. Maizel, Plant Sciences Institute Short Talk: miR390, TAS3 ta-siRNAs and ARF3/4 Define a Self-Regulatory Network Controlling Quantitatively Lateral Root Growth
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Mesa Ballroom
7:30 - 10:00 PM	Poster Session 2	Mesa C/Chamisa
Wednesday, February 24
7:00 - 8:00 AM	Breakfast	Chamisa/Ortiz
8:00 - 11:15 AM	Integration of RNA Silencing and RNA Metabolism	Mesa Ballroom
	* Steven E. Jacobsen, HHMI/University of California, Los Angeles
	Xuemei Chen, University of California, Riverside Three Polymerases in siRNA-Directed Transcriptional Gene Silencing
	Craig S. Pikaard, Washington University Roles of Pol IV and Pol V Transcription in RNA Silencing
	Caroline Dean, John Innes Centre Targeted 3’ Processing of Antisense Transcripts Triggers Arabidopsis FLC Chromatin Silencing
	Pamela J. Green, Delaware Biotechnology Institute Analysis of miRNAs and Target RNAs
	Hailing Jin, University of California, Riverside Short Talk: Host Small RNAs and RNAi Machinery Play Important Roles In Plant Immunity
	Michael D. Nodine, Whitehead Institute Short Talk: SPL Transcription Factor Transcripts are Cleared from the Early Embryo by miR156 to Prevent Premature Differentiation and to Facilitate Pattern Formation
9:20 - 9:40 AM	Coffee Break	Promenade
11:15 AM -	On Own for Lunch and Recreation
4:30 - 5:00 PM	Coffee Available	Promenade
5:00 - 7:00 PM	Interaction of Pathogens and RNA Silencing Mechanisms	Mesa Ballroom
	* Hailing Jin, University of California, Riverside
	Shou-Wei Ding, University of California, Riverside Viral Secondary siRNAs in Antiviral Silencing
	Jozsef Burgyán, Plant Virology Institute-CNR Viral Suppressors of RNA Silencing
	Michael Metzlaff, Bayer BioScience N.V. RNAi and Epigenetics for Crop Improvement
	Mathew G. Lewsey, University of Cambridge Short Talk: Disruption of Jasmonate and Salicylate-Regulated Defensive Signaling Pathways by the 2b RNA Silencing Suppressor Protein Encoded by Cucumber Mosaic Virus
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Mesa Ballroom
Thursday, February 25
7:00 - 8:00 AM	Breakfast	Chamisa/Ortiz
8:00 - 11:15 AM	Genome-Wide Technologies and Applications of RNA Silencing	Mesa Ballroom
	* Craig S. Pikaard, Washington University
	Blake C. Meyers, University of Delaware Genome-Wide Analyses of Plant Small RNAs

	Ronan C. O'Malley, The Salk Institute Connecting Genetic and Epigenetic Variation in 1,001 Arabidopsis Genomes
	Michael J. Axtell, Pennsylvania State University Discovery of Small RNA Targets and MIRNA Processing Pathways by "Degradome" Sequencing
	Gregory Heck, Monsanto Co. Commercial Applications of Gene Silencing
	Casey Richardson, Texas Tech University Short Talk: Computational Analysis of Antisense Transcription using Arabidopsis Whole Genome Tiling Microarray and Small RNA Abundance Data Suggests Mis-Annotation of Predicted ‘Orphan’ Protein-Coding Genes
9:20 - 9:40 AM	Coffee Break	Promenade
11:15 AM -	On Own for Lunch and Recreation
4:30 - 5:00 PM	Coffee Available	Promenade
5:00 - 7:00 PM	Natural Variation and RNA Silencing	Mesa Ballroom

	* Michael J. Axtell, Pennsylvania State University
	Magnus Nordborg, Gregor Mendel Institute Analyzing the Genotype-Phenotype Map in Arabidopsis

	Xiaofeng Cao, Institute of Genetics and Developmental Biology Characterization of Dicer-Like Proteins Reveals Novel phased siRNAs Pathways in Rice
	Robert Martienssen, Cold Spring Harbor Laboratory Natural Variation in Small RNA-Regulation of Plant Genes
	Lisa M. Smith, Max Planck Institute for Developmental Biology Short Talk: Natural Variation of Small RNA Populations and Transcriptomes
8:00 - 9:00 PM	Social Hour w/ Lite Bites	Mesa Ballroom
8:00 - 11:00 PM	Entertainment	Mesa Ballroom
Friday, February 26
	Departure

*Session Chair †Speaker invited, not yet responded.