Keystone Symposia | Scientific Conferences on Biomedical and Life Science Topics

The Many Faces of Ubiquitin (A4)

Organizer(s): Simona Polo, Christopher D. Lima and Vishva M. Dixit
January 11 - 16, 2009
Copper Mountain Resort · Copper Mountain, Colorado
Abstract Deadline: September 17, 2008
Late Abstract Deadline: October 14, 2008
Scholarship Deadline: September 17, 2008
Early Registration Deadline: November 11, 2008

Supported by the Directors' Fund

This meeting took place in the 2009 season.

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Summary of Meeting
The ubiquitin (Ub) pathway controls many biological processes and determines how cells respond to growth factors, stress and genetic damage, controlling nearly every facet of a cell’s life and death. The covalent attachment of Ub to proteins can alter their localization, activities, and ultimate fate. One of the most intriguing features of the ubiquitin pathway is its emerging involvement in functions not directly related to protein degradation. In addition, some half-dozen related ubiquitin-like (Ubl) proteins, such as SUMO, are also revealing themselves as regulators of numerous cellular pathways. The existence of this vast and dynamic array of Ub and Ubl signals raises the central question of how specificity is achieved during conjugation, recognition, and signal transduction. Indeed, the molecular requirements for generating and recognizing various Ub signals are poorly understood and form a central unresolved issue in the field. This meeting will focus attention on the biology associated with Ub/Ubl-mediated signal transduction, including its role in human disease and cancer. The goal of this meeting is to gather a diverse array of scientists, not just “ubiquitinologists”, but also biologists whose work has been touched in some way by ubiquitin or ubiquitin-like pathways. By bringing together scientists from different backgrounds who work on a wide range of systems that are regulated by Ub and Ubl conjugation, this meeting will create a fertile ground for exchange of ideas on non-conventional functions for Ub and Ubl proteins in signal transduction.

Sunday, January 11
3:00 - 7:30 PM	Registration	Kokopelli's
6:30 - 7:30 PM	Refreshments	Kokopelli's
7:30 - 7:45 PM	Opening Remarks
	* Vishva M. Dixit, Genentech, Inc.
7:45 - 8:45 PM	The Cecile Pickart Memorial Lecture	Big Horn B
	Daniel J. Finley, Harvard University Regulation of Substrate Processing by the Proteasome

Monday, January 12
7:00 - 8:00 AM	Breakfast	Kokopelli's
8:00 - 11:15 AM	Signal Transduction by Ub (I)	Big Horn B

	* Brenda A. Schulman, St. Jude Children's Research Hospital

	Vishva M. Dixit, Genentech, Inc. Usp9x: A Stabilizing Deubiquitinase for Mcl-1
	David O. Morgan, University of California, San Francisco Substrate Recognition by the Anaphase-Promoting Complex
	J. Wade Harper, Harvard Medical School Genetic and Proteomic Analysis of the Ubiquitin System
	Claudio A.P. Joazeiro, The Scripps Research Institute Mitochondria and the Ubiquitin System
	Sirio Dupont, University of Padova Short Talk: A Deubiquitinating Enzyme Essential for TGFbeta Signaling Controls Smad4 Monoubiquitination
	Peter Kaiser, University of California, Irvine Short Talk: Inactivation of SCF by Signal-Induced Disassembly
9:20 - 9:40 AM	Coffee Break	Kokopelli's
11:15 AM - 1:00 PM	Poster Setup	Big Horn C
11:15 AM -	On Own for Lunch
1:00 - 10:00 PM	Poster Viewing	Big Horn C
4:30 - 5:00 PM	Coffee Available	Kokopelli's
5:00 - 7:00 PM	Signal Transduction by Ub (II)	Big Horn B
	* Ronald T. Hay, University of Dundee
	Simona Polo, FIRC Institute of Molecular Oncology Ubiquitin in Endocytosis and Signaling of EGFR
	Morag Park, McGill University Met Receptor Tyrosine Kinase: Intracellular Trafficking, Signal Polarisation and Transformation
	Zhijian James Chen, University of Texas Southwestern Medical Center and
	Ming Xu, University of Texas Southwestern Medical Center Ubiquitin in the NF-kappaB Pathway
	Mads Gyrd-Hansen, University of Copenhagen Short Talk: IAPs Contain an Evolutionarily Conserved Ubiquitin-Binding Domain that is Essential for NF-kappaB Regulation and Oncogenesis
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Kokopelli's
7:30 - 10:00 PM	Poster Session 1	Big Horn C
Tuesday, January 13
7:00 - 8:00 AM	Breakfast	Kokopelli's
8:00 - 11:15 AM	Enzymes Regulation	Big Horn B
	* Morag Park, McGill University
	Raymond J. Deshaies, California Institute of Technology Multimodal Activation of the Ubiquitin Ligase SCF by Nedd8 Conjugation
	Brenda A. Schulman, St. Jude Children's Research Hospital Mechanisms and Specificity of NEDD8ylation
	Anindya Dutta, University of Virginia CRL4-Cdt2 in the Maintenance of Genome Stability
	Jonathon Pines, University of Cambridge Short Talk: How is the APC/C-Cdc20 Complex Regulated in Mitosis?
	Yang Xie, Yale University Short Talk: Proteolytic Regulation of the Yeast MATalpha2 Transcription Factor by a SUMO-Targeted Ubiquitin Ligase (STUbL)
	R. Andrew Byrd, National Cancer Institute, National Institutes of Health Short Talk: Allosteric Activation of Ubiquitin Ligase Activity by a Novel E2 Binding Region
9:20 - 9:40 AM	Coffee Break	Kokopelli's
11:15 AM - 1:00 PM	Poster Setup	Big Horn C
11:15 AM -	On Own for Lunch
1:00 - 10:00 PM	Poster Viewing	Big Horn C
2:30 - 4:30 PM	Workshop 1: Tools and Reagents Development	Big Horn B
	* Paul W. Sheppard, BIOMOL International Inc State of the Art
	Christopher Tsu, Millennium Pharmaceuticals Development of a Novel Charcoal Paper ATP: Pyrophosphate Exchange Assay: Kinetic Characterization of NEDD8 Activating Enzyme
	Karen M. Kleman-Leyer, Bellbrook Labs Monitoring AMP Flux through the SUMOylation Cascade Using the Transcreener AMP/GMP Assay
	Michael H. Tatham, University of Dundee System-Wide Analysis of Changes to the SUMO Proteome in Response to External Stimuli
	Paul S. Andrews, Amgen Inc Identification of SMURF1 Substrates Utilizing Protein Microarrays

	Birte Kalveram, University of Constance Generation and Characterization of Monoclonal Antibodies Against the Ubiquitin-Like Modifier FAT10

4:30 - 5:00 PM	Coffee Available	Kokopelli's
5:00 - 7:00 PM	Various Chains, Various Signals	Big Horn B
	* Raymond J. Deshaies, California Institute of Technology
	Kazuhiro Iwai, Osaka University Physiological Function of Linear Polyubiquitin Chains
	Michael Rape, University of California, Berkeley The Assembly of K11-linked Ubiquitin Chains during Mitosis
	Junmin Peng, Emory University Analysis of Ubiquitinated Proteins and PolyUb Linkages by Quantitative Mass Spectrometry

7:00 - 8:00 PM	Social Hour w/ Lite Bites	Kokopelli's
7:30 - 10:00 PM	Poster Session 2	Big Horn C
Wednesday, January 14
7:00 - 8:00 AM	Breakfast	Kokopelli's
8:00 - 11:15 AM	UBDs and UBLs Function and Specificity	Big Horn B
	* Zhijian James Chen, University of Texas Southwestern Medical Center
	Ivan Dikic, Goethe University Medical School Specificity in UbiquitinSignaling Networks

	Kylie J. Walters, University of Minnesota The Proteasome‘s Ubiquitin Receptors

	Katerina Heran Darwin, New York University School of Medicine Discovery of a Ubiquitin-Like Protein Involved in the Proteasome Pathway of Mycobacterium tuberculosis
	Frauke Melchior, University of Goettingen Characterisation of the RanBP2 / RanGAP1SUMO1 E3 Ligase Complex*
	Christian D. Schlieker, Whitehead Institute for Biomedical Research Short Talk: A Functional Proteomics Approach Links the Ubiquitin-Related Modifier Urm1 to a RNA Modification Pathway
	Kate E. Stoll, University of Washington Short Talk: Interaction of the E2s yeast Ubc4 and Human UbcH5 with Ub: Functional Consequence.
9:20 - 9:40 AM	Coffee Break	Kokopelli's
11:15 AM - 1:00 PM	Poster Setup	Big Horn C
11:00 AM -	On Own for Lunch
1:00 - 10:00 PM	Poster Viewing	Big Horn C
2:30 - 4:30 PM	Workshop 2: Animal Models	Big Horn B
	* Ron R. Kopito, Stanford University Ubiquitin Homeostasis on the Edge: Lessons from Polyubiquitin Knockout Mice

	Pengbo Zhou, Weill Cornell Medical College CULLIN 4A Knockout Mice
	Nicholas John van Buuren, University of Alberta A Novel Family Of Viral Ankyrin/F-Box Proteins Interact With The SCF Complex
	Maud Demarque, Pasteur Institute A Mouse Model for the Study of Sumo E2 Function
	Michael Kuehn, National Cancer Institute, National Institutes of Health Loss of SUMO-1 in Mice is not Lethal Due to Compensation by SUMO-2/3
	Jongdae Lee, University of California, San Diego STAT3 Maintains Intestinal Epithelial Barrier by Induction of Ubiquitin-Mediated Degradation of SNAI
	Birgit Aerne, Cancer Research UK A Uiquitin-Related RNAi Screen in Drosophila
4:30 - 5:00 PM	Coffee Available	Kokopelli's
5:00 - 7:00 PM	Signal Transduction by UbIs	Big Horn B
	* Frauke Melchior, University of Goettingen

	Deborah J. Lenschow, Washington University School of Medicine Regulation of the Host Antiviral Response by ISG15
	Edward T.H. Yeh, University of Texas MD Anderson Cancer Center Role of Sentrin/SUMO-Specific Protease 1 (SENP1) in Angiogenesis
	Ronald T. Hay, University of Dundee Regulation of Promyelocytic Leukaemia Protein by SUMO
	Michael N. Boddy, The Scripps Research Institute Short Talk: New Interactions of SUMO Pathway Enzymes in Genome Stability
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Kokopelli's
7:30 - 10:00 PM	Poster Session 3	Big Horn C
Thursday, January 15
7:00 - 8:00 AM	Breakfast	Kokopelli's
8:00 - 11:15 AM	Ubiquitin and UbIs in Cancer	Big Horn B
	* Alan D. D'Andrea, Dana-Farber Cancer Institute
	Ingrid E. Wertz, Genentech, Inc. Ubiquitin Editing
	Marcus Groettrup, University of Constance The Role of FAT10 in Cancer

	Lawrence R. Dick, Millennium Pharmaceuticals Targeting the NEDD8 Conjugation Pathway for Cancer Chemotherapy


	Roger A. Greenberg, University of Pennsylvania School of Medicine Ubiquitin Recognition and Turnover in BRCA1-Dependent DNA Repair and Tumor Suppression
	Iannis Aifantis, HHMI/New York University School of Medicine Short Talk: The Fbw7 E3 Ubiquitin Ligase Controls Hematopoietic Stem Cell Self-Renewal and Transformation
	Lian Li, Emory University School of Medicine Short Talk: Parkin-Mediated Ubiquitin Signaling in Regulation of the Aggresome-Autophagy Pathway
9:20 - 9:40 AM	Coffee Break	Kokopelli's
11:15 AM -	On Own for Lunch
4:30 - 5:00 PM	Coffee Available	Kokopelli's
5:00 - 6:25 PM	Regulation by Ub and UbIs in the Nucleus	Big Horn B
	* Edward T.H. Yeh, University of Texas MD Anderson Cancer Center
	Jesper Q. Svejstrup, Cancer Research UK London Research Institute Ubiquitylation of RNA Polymerase II


	Alan D. D'Andrea, Dana-Farber Cancer Institute Regulation of DNA Repair by Monoubiquitination
	Valentina Perissi, University of California, San Diego Short Talk: Dismissal of Distinct Corepressor Complexes from Regulated Gene Promoters is Regulated by Specific Phosphorylation of the TBL1 and TBLR1 Exchange Factors
6:25 - 7:15 PM	Translational Research Lecture	Big Horn B
	Giulio F. Draetta, Dana-Farber Cancer Institute Druggable Targets in the Ubiquitin Field
7:15 - 7:30 PM	Closing Remarks
	Simona Polo, FIRC Institute of Molecular Oncology
7:30 - 8:30 PM	Social Hour w/ Lite Bites	Kokopelli's
8:30 - 11:30 PM	Entertainment	Big Horn C
Friday, January 16
	Departure

*Session Chair †Speaker invited, not yet responded.