Granlibakken Tahoe Floorplan

Registered Attendees


Registered attendees (and speakers, organizers, etc.) will have access to the following items from their Account page:

  • Abstracts from speakers and poster sessions, including the joint meeting abstracts, available 30 days prior to the meeting (You can edit your own abstract from My Account page as well)

    NOTE: Abstract authors/submitters may choose to not have their abstract available online and in the secure mobile app until a week before the meeting.

  • Full participant list, including joint meeting participants
  • Printable Invoices and Invitation Letters
  • Scholarship Information
  • Lodging Information

Login to My Account page

This meeting took place in 2015



For a complete list of the meetings for the upcoming/current season, see our meeting list, or search for a meeting.

Hybrid Methods in Structural Biology (C2)


Organizer(s) Jens Meiler, Patrick Cramer and Ron A. Milligan
March 4—8, 2015
Granlibakken Tahoe • Tahoe City, California USA
Discounted Abstract Deadline: Nov 6, 2014
Abstract Deadline: Dec 9, 2014
Scholarship Deadline: Nov 6, 2014
Discounted Registration Deadline: Jan 8, 2015

Sponsored by Vertex Pharmaceuticals Incorporated

Summary of Meeting:
As we study the structure and dynamics of more and more complex systems to better understand their biological function, each individual technology in the structural biology toolbox approaches its limitations. These limitations include size of the system, timeline of motions, spatial and/or temporal resolution and ensemble averaging. Further, each individual method perturbs the system in a different way to enable or facilitate its analysis. This includes, for example, the introduction of spin labels to perform EPR experiments, the reconstitution of membrane proteins in micelles for NMR spectroscopy, the introduction of entire domains to stabilize proteins in certain conformations for crystallization, or a simplified force field in computation. As systems become more challenging, the perturbations tend to get more drastic, complicating the interpretation of results. Only at the intersection of several techniques does it become possible to distinguish information that is biologically relevant from perturbations introduced by any individual approach. Moving forward, hybrid methods and integrated methods in structural biology play a key role in studying complex systems verifying each other’s results. The objective of this Keystone Symposia meeting is two-fold: 1) To discuss novel and potentially disruptive technologies and/or combinations thereof to be added to the hybrid methods toolbox; and 2) To illustrate and discuss application of hybrid methods to important biological systems. These objectives will be achieved through oral presentations, poster sessions, discussions in panel format and tutorial sessions introducing new software algorithms.

View Scholarships/Awards
No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 12 hr (am/pm) time


WEDNESDAY, MARCH 4

16:00—20:00
Arrival and Registration

Pre Function
18:00—20:00
Welcome Mixer
No registration fees are used to fund alcohol served at this function.

Granhall
18:00—20:00
Light Meal - Provided by Granlibakken

Granhall

THURSDAY, MARCH 5

07:00—08:00
Breakfast

Granhall
08:00—09:00
Keynote Address
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Jens Meiler, Vanderbilt University, USA

Ad Bax, NIDDK, National Institutes of Health, USA
When NMR Alone Is Not Enough

09:00—11:30
New and Potentially Disruptive Technologies: X-Rays, Neutrons, Electrons
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
Richard Neutze, University of Gothenburg, Sweden
Serial Femtosecond Crystallography and Time-Resolved Wide-Angle X-Ray Scattering Studies of a Photosynthetic Reaction Center at an X-Ray Free Electron Laser

Christian Betzel, University of Hamburg, Germany
Latest Methods to Grow, Prepare and Score Micro- and Nano-Crystals for Future Free-Electron-Laser and Synchrotron Radiation Sources

Osamu Miyashita, RIKEN, Japan
Short Talk: Hybrid Approach for X-Ray Free Electron Laser Single Particle Analysis of Biomolecular Systems

Basil Greber, ETH Zürich, Switzerland
Short Talk: Insights into Structure and Function of the Mammalian Mitochondrial Ribosome

Stefan Raunser, Max Planck Institute of Molecular Physiology, Germany
How to Kill a Mocking Bug - Structural Insights into Tc Toxin Complex Action

09:40—10:00
Coffee Break

Pre Function
11:30—13:00
Poster Setup

Bay
13:00—22:00
Poster Viewing

Bay
11:30—17:00
On Own for Lunch

14:30—16:30
Panel Discussion 1

Mountain/Lake
* Andrej Sali, University of California, San Francisco, USA

Charles H. Greenberg, University of California, San Francisco, USA
Bayesian Modeling of Macromolecular Structures Based on Electron Microscopy Density Maps

Abhishek Singharoy, Arizona State University, USA
Structure Determination with Data-Guided Molecular Dynamics

Stephan Niebling, University of Gothenburg, Sweden
Coarse-Grained Protein X-Ray Scattering Calculations for the Analysis of Time-Resolved X-Ray Scattering

Lam T. Nguyen, California Institute of Technology, USA
Coarse-Grained Simulations Reveal Mechanisms of Bacterial Cell Wall Synthesis

Steffen Lindert, University of California, San Diego, USA
CryoEM-Guided Iterative Molecular Dynamics – Rosetta Protein Structure Refinement Protocol Improves Protein Model Quality

Graham T. Johnson, University of California, San Francisco, USA
Towards Whole Cells Modeled in 3D Molecular Detail and Community Curated with cellPACK

Clarence Y. Cheng, Stanford University, USA
Consistent and Blind Inference of RNA Tertiary Folds from Multidimensional Chemical Mapping Experiments

Michiel J. van de Waterbeemd, Utrecht University, Netherlands
Structure and Activity of the RNA Polymerase II - Capping Enzyme Complex Probed by Native Mass Spectrometry and other Structural Biology Methods

16:30—17:00
Coffee Available

Pre Function
17:00—19:00
Membrane Proteins
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
Andre Hoelz, California Institute of Technology, USA
Architecture of the Nuclear Pore Complex Coat

Matthew T. Eddy, University of Southern California, USA
Short Talk: Conformational Dynamics of G Protein-Coupled Receptors Studied by 19F NMR and Novel Isotopic Labeling Approaches

Leo Sazanov, Medical Research Council, UK
Structure and Mechanism of Respiratory Complex I

* Lukas K. Tamm, University of Virginia, USA
Hybrid Structural Approaches to Resolve the Build-Up of the Exocytotic Fusion Pore

19:00—20:00
Dinner

Granhall
19:30—22:00
Poster Session 1

Bay
20:00—21:00
Social Hour
No registration fees are used to fund alcohol served at this function.

Bay

FRIDAY, MARCH 6

07:00—08:00
Breakfast

Granhall
08:00—11:00
New and Potentially Disruptive Technologies: Spectroscopy and Spectrometry
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Robert Kaptein, University of Utrecht, Netherlands

Dorothee Kern, Brandeis University, HHMI, USA
Ancestor Resurrection as a Microscope into a Cancer Drug’s Mechanism

Mathias Ferber, Institut Pasteur, France
Short Talk: Integrating the Solvent Accessible Surface Distance with Cross-Links-Based Modeling Methods Improves the Conformational Sampling of Protein Assemblies

Thomas Huber, Australian National University, Australia
Protein Structure Determination from Sparse Pseudocontact Shifts

Gunnar Jeschke, ETH Zürich, Switzerland
Structure and Flexibility of Soluble and Membrane Proteins from Sparse Experimental Data

Florian Stengel, ETH Zurich, Switzerland
Short Talk: Chemical Cross-Linking/Mass Spectrometry and the Structural Biology Toolbox

Judy E. Kim, University of California, San Diego, USA
Resonance Raman and FRET Studies of a Beta-Barrel Membrane Protein

09:20—09:40
Coffee Break

Pre Function
11:00—13:00
Poster Setup

Bay
13:00—22:00
Poster Viewing

Bay
11:00—17:00
On Own for Lunch

13:30—16:30
Workshop 1: Integrated Modeling with the Integrative Modeling Platform
The URL for this workshop is: http://integrativemodeling.org/keystone2015/

Granhall
* Andrej Sali, University of California, San Francisco, USA

Benjamin Webb, University of California, San Francisco, USA

Dina Schneidman, University of California, San Francisco, USA

Charles H. Greenberg, University of California, San Francisco, USA

Seung Joong Kim, University of California, San Francisco, USA

13:30—16:30
Workshop 2: Protein Structure Determination Form Limited Experimental Data with Rosetta
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/

Mountain/Lake
* Jens Meiler, Vanderbilt University, USA

Rocco Moretti, Vanderbilt University, USA

Frank DiMaio, University of Washington, USA

Nikolaos G. Sgourakis, University of California, Santa Cruz, USA

16:30—17:00
Coffee Available

Pre Function
17:00—19:00
Electron Microscopy
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Alasdair C. Steven, NIAMS, National Institutes of Health, USA

Elizabeth Villa, University of California, San Diego, USA
Opening Windows into the Cell: Cryo-Electron Tomography of Intact Cells for Structural Cell Biology

Melody G. Campbell, The Scripps Research Institute, USA
Short Talk: Automated Near Atomic Resolution Electron Microscopy

Frank DiMaio, University of Washington, USA
Toward Automatic Structure Determination from Near-Atomic-Resolution cryoEM Density Maps

Yifan Cheng, University of California, San Francisco, USA
Study Membrane Protein Structures by Single Particle cryo-EM

19:00—20:00
Dinner

Granhall
19:30—22:00
Poster Session 2

Bay
20:00—21:00
Social Hour
No registration fees are used to fund alcohol served at this function.

Bay

SATURDAY, MARCH 7

07:00—08:00
Breakfast

Granhall
08:00—11:00
Supramolecular Assemblies
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Frank DiMaio, University of Washington, USA

Patrick Cramer, Max Planck Institute for Biophysical Chemistry, Germany
Integrated Structural Biology of Genome Transcription

Andrew B. Ward, The Scripps Research Institute, USA
Short Talk: Viral Glycoprotein Antibody Complexes

Andrea Graziadei, EMBL Heidelberg, Germany
Short Talk: Investigation of the Methylation Mechanism of the Box C/D Ribonucleoprotein Complex

Alasdair C. Steven, NIAMS, National Institutes of Health, USA
Mapping Components of Macromolecular Complexes in Two and Three Dimensions by Bubblegram Imaging

Juli Feigon, University of California, Los Angeles, USA
The Architecture of Tetrahymena Telomerase Holoenzyme

Martin Beck, European Molecular Biology Laboratory, Germany
Integrated Structural Analysis of the Human Nuclear Pore Complex

09:20—09:40
Coffee Break

Pre Function
11:00—17:00
On Own for Lunch

14:30—16:30
Panel Discussion 2

Mountain/Lake
* Patrick Cramer, Max Planck Institute for Biophysical Chemistry, Germany

Philip Lössl, Utrecht University, Netherlands
FIRM – An Integrated Mass Spectrometric Approach to Study the Impact of Protein Phosphorylation on the Dynamic Interactions of Mitotic Regulators

Vladimir Svetlov, New York University Langone Medical Center, USA
Short Talk: Docking Model of E. coli RNA Polymerase Complex with Transcription Factor NusA Guided by in vivo Chemical Cross-Links Mapped by Mass Spectrometry

Carolina Sánchez-Rico, Technical University of Munich, Germany
Study of the Conformational Dynamics of the Splicing Factor of U2AF65

Sebastian Westenhoff, University of Gothenburg, Sweden
Signal Transduction in Phytochrome Photosensors Visualized by Time-Resolved X-Ray Scattering

Thomas U. Schwartz, Massachusetts Institute of Technology, USA
Atomic Structure of the Y-Complex of the Nuclear Pore

Audray K. Harris, NIAID, National Institutes of Health, USA
Characterization of Influenza Virus Subunit Vaccines by Electron Microscopy

Alexey Amunts, Stockholm University, Sweden
Structure of Human Mitochondrial Ribosome Determined by cryo-EM

Yuehan Feng, ETH Zürich, Switzerland
Global Analysis of Protein Structural Changes in Complex Proteomes

16:30—17:00
Coffee Available

Pre Function
17:00—18:45
New and Potentially Disruptive Technologies: Computation
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Michael Nilges, Institut Pasteur, France

Sarah Teichmann, Wellcome Trust Sanger Institute, UK
A Periodic Table of Protein Complexes

Nikolaos G. Sgourakis, University of California, Santa Cruz, USA
Modeling Supramolecular Assemblies Using NMR and cryoEM Data

Jens Meiler, Vanderbilt University, USA
Protein Structure Determination by Integrating Limited Experimental Data

18:45—19:15
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)

Mountain/Lake
19:15—20:15
Dinner

Granhall
19:15—20:15
Social Hour
No registration fees are used to fund alcohol served at this function.

Granhall
20:00—23:00
Entertainment
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Granhall

SUNDAY, MARCH 8

 
Departure


*Session Chair †Invited, not yet responded.



We gratefully acknowledge support for this conference from:


Directors' Fund


These generous unrestricted gifts allow our Directors to schedule meetings in a wide variety of important areas, many of which are in the early stages of research.

Click here to view all of the donors who support the Directors' Fund.



Keystone Symposia thanks our Sponsor for generously supporting this meeting:

Vertex Pharmaceuticals Incorporated

We gratefully acknowledge the generous grant for this conference provided by:


National Institutes of Health

Grant No. 1R13GM113567-01

The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention of trade names, commercial practices, or organizations imply endorsement by the U.S. Government.


We gratefully acknowledge additional support for this conference from:

ISOTEC, a member of the Sigma-Aldrich Group
New Era Enterprises, Inc.


We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:


Click here to view more of these organizations


Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:


Click here to view more of these organizations


If your organization is interested in joining these entities in support of Keystone Symposia, please contact: Sarah Lavicka, Director of Development, Email: sarahl@keystonesymposia.org,
Phone:+1 970-262-2690

Click here for more information on Industry Support and Recognition Opportunities.

If you are interested in becoming an advertising/marketing in-kind partner, please contact:
Yvonne Psaila, Director, Marketing and Communications, Email: yvonnep@keystonesymposia.org,
Phone:+1 970-262-2676