Keystone Symposia | Scientific Conferences on Biomedical and Life Science Topics

RNA Silencing: Mechanism, Biology and Application (A7)

Organizer(s): Phillip D. Zamore and Beverly L. Davidson
January 14 - 19, 2010
Keystone Resort · Keystone, Colorado
Abstract Deadline: September 17, 2009
Late Abstract Deadline: October 15, 2009
Scholarship Deadline: September 17, 2009
Early Registration Deadline: November 16, 2009

Sponsored by Alnylam Pharmaceuticals, Inc., Isis Pharmaceuticals, Inc., Regulus Therapeutics, Inc. and Santaris Pharma A/S

This meeting took place in the 2010 season.

Listed below are current meetings that are similar to this meeting in nature/content:

MicroRNAs and Human Disease (J6)
February 11 - 16, 2011
Mechanism and Biology of Silencing (C7)
March 20 - 25, 2011

For a complete list of the meetings for the upcoming/current season,
see our meeting list, or search for a meeting.

Summary of Meeting
Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small RNA classes have been identified, including microRNAs, various types of siRNAs and piRNAs. These classes differ in their biogenesis, modes of target regulation and in the biological pathways they regulate. However, there is also a growing realization that these distinct small RNA pathways are interconnected. These pathways functions in RNA-based immunity, developmental gene regulation, transcriptional silencing, and genomic stability. The aim of this meeting is to explore the similarities and differences between RNA silencing pathways, to probe their underlying molecular mechanisms, to learn—by illustrative examples—the logic behind RNA-guided regulation of gene expression, and to explore new methods for harnessing these mechanisms for gene discovery and therapeutics applications.

Thursday, January 14
3:00 - 7:30 PM	Registration	Longs Peak Foyer
6:30 - 7:30 PM	Refreshments	Longs Peak Foyer
7:30 - 9:30 PM	Keynote Session	Longs/Quandary Peaks
	James C. Carrington, Oregon State University Small RNA-Directed Silencing Pathways in Plants
	V. Narry Kim, Seoul National University The Making of a MicroRNA
Friday, January 15
7:00 - 8:00 AM	Breakfast	Grays/Torreys Peaks
8:00 - 11:00 AM	Structure and Regulation of Small RNAs	Longs/Quandary Peaks

	Jennifer A. Doudna, University of California, Berkeley Dicer and Beyond: Mechanisms of Regulatory RNA Processing and Targeting
	Rachel J. Senturia, University of California, Los Angeles Short Talk: Crystallographic and Cellular Studies of the DGCR8 Dimerization Domain: A Platform for Interaction with Heme
	Dinshaw J. Patel, Memorial Sloan-Kettering Cancer Center A Structural Perspective of Recognition and Cleavage Events in RNA Silencing


	Nina Schürmann, University of Heidelberg Short Talk: Directed Molecular Evolution of Chimeric Argonaute Proteins as Potent New Approach to Elucidate Structure-Function Relationships of Human RISC
	Martijn Kedde, Netherlands Cancer Institute Short Talk: A Pumilio-Induced RNA Structure Switch in p27-3’UTR Controls miR-221&222 Accessibility
9:20 - 9:40 AM	Coffee Break	Longs Peak Foyer
11:00 AM -	On Own for Lunch and Recreation
11:00 AM - 1:00 PM	Poster Setup	Shavano/Torreys Peaks
1:00 - 10:00 PM	Poster Viewing	Shavano/Torreys Peaks
4:30 - 5:00 PM	Coffee Available	Longs Peak Foyer
5:00 - 7:15 PM	Regulation of Small RNA Pathways	Longs/Quandary Peaks
	Brenda L. Bass, University of Utah A Role for Dicer's Helicase Domain
	Brandi N. Davis, Tufts Medical Center Short Talk: Smad Proteins Bind a Conserved RNA Sequence to Promote microRNA Maturation by Drosha
	Olivier Voinnet, Institut de Biologie Moléculaire des Plantes Function and Regulation of miRNA Pathways in Plants
	Gary B. Ruvkun, Massachusetts General Hospital, Harvard Medical School Genetic Analysis Reveals the Complexity of the C. elegans RNA Silencing Pathways
	Guillaume Jannot, Laval University Cancer Research Centre Short Talk: Conserved Function of a Ribosomal Protein RACK1 in the microRNA Pathway
7:15 - 8:15 PM	Social Hour w/ Lite Bites	Shavano/Torreys Peaks
7:30 - 10:00 PM	Poster Session 1	Shavano/Torreys Peaks
Saturday, January 16
7:00 - 8:00 AM	Breakfast	Grays/Torreys Peaks
8:00 - 11:15 AM	miRNA Biogenesis and Function	Longs/Quandary Peaks
	Xuemei Chen, University of California, Riverside Three Polymerases in siRNA-Directed Transcriptional Gene Silencing
	Elisa Izaurralde, MPI for Developmental Biology The silencing domain of GW182 interact with PABPC1 to prompt silencing of miRNA targets
	Demian E. Cazalla, Yale University Short Talk: Herpesvirus saimiri U RNAs Affect the Expression of Cellular microRNAs in Latently Infected T Cells
	David P. Bartel, Massachusetts Institute of Technology MicroRNAs: Genomics, Targets, and the Molecular Consequences of Targeting
	Victor R. Ambros, University of Massachusetts Modulators of microRNA Activity
	Catherine A. Reinke, Northwestern University Short Talk: Elucidation of the miRNA Pathway through the Identification and Characterization of Genes Identified in a Forward Genetics Study of miRNA Function in Drosophila melanogaster
9:20 - 9:40 AM	Coffee Break	Longs Peak Foyer
11:15 AM -	On Own for Lunch and Recreation
11:15 AM - 1:00 PM	Poster Setup	Shavano/Torreys Peaks
1:00 - 10:00 PM	Poster Viewing	Shavano/Torreys Peaks
4:30 - 5:00 PM	Coffee Available	Longs Peak Foyer
5:00 - 7:30 PM	Small RNA Therapeutics	Longs/Quandary Peaks
	Priti Kumar, Yale School of Medicine Targeting siRNAs to T-Cells for Anti-Viral Therapy

	Daniel G. Anderson, Massachusetts Institute of Technology Combinatorial Development of Synthetic siRNA Delivery Systems
	Mark A. Kay, Stanford University Mechanism of MicroRNA Mediated Transgene Silencing
	Lukas Jeker, University of California San Francisco Short Talk: miR-10a in Regulatory T Cells
	Sakari Kauppinen, Santaris Pharma Targeting of microRNAs for Therapeutics

7:30 - 8:30 PM	Social Hour w/ Lite Bites	Shavano/Torreys Peaks
7:30 - 10:00 PM	Poster Session 2	Shavano/Torreys Peaks
Sunday, January 17
7:00 - 8:00 AM	Breakfast	Grays/Torreys Peaks
8:00 - 11:15 AM	Small RNAs and RNAi I	Longs/Quandary Peaks

	Attila Molnar, University of Cambridge Small RNAs are Mobile and Trigger DNA Methylation in Distant Tissue


	Sarah C.R. Elgin, Washington University Targeting Heterochromatin Formation in Drosophila
	Hanbang Zhang, Stanford University Short Talk: Antisense Small RNAs with 5’-Polyphosphate Termini Regulate Gene Expression in the Deep Branching Eukaryote Entamoeba Histolytica
	Phillip A. Sharp, Massachusetts Institute of Technology MicroRNAs in mammalian cells---robustness
	Elaine Fuchs, HHMI/Rockefeller University Regulation in skin stem cell: Fine tuning by miRNA profile
	Shouhong Guang, University of Wisconsin, Madison Short Talk: Small RNAs Mediate Transcription Termination
9:20 - 9:40 AM	Coffee Break	Longs Peak Foyer
11:15 AM -	On Own for Lunch and Recreation
11:15 AM - 1:00 PM	Poster Setup	Grays/Torreys Peaks
1:00 - 10:00 PM	Poster Viewing	Grays/Torreys Peaks
2:30 - 4:30 PM	Workshop: piRNA Biogenesis and Function	Longs/Quandary Peaks
	Craig C. Mello, University of Massachusetts Medical School piRNAs in Worms: The Same, but Different
	Mikiko C. Siomi, Keio University School of Medicine piRNAs in the Fly Germ Line
	Gregory J. Hannon, Cold Spring Harbor Laboratory Why is Ago catalysis conserved in vertebrate?
	Phillip D. Zamore, University of Massachusetts Medical School Target directed destruction of small silencing RNAs
4:30 - 5:00 PM	Coffee Available	Longs Peak Foyer
5:00 - 7:00 PM	Small RNAs and RNAi II	Longs/Quandary Peaks

	René F. Ketting, Hubrecht Institute Piwi biology in Zebrafish
	Danesh Moazed, Harvard Medical School Small RNAs that Trigger Heterochromatin Assembly and RNAi
	Shiv I. S. Grewal, NCI, National Institutes of Health RNAi and Heterochromatin Factors Suppress Antisense RNAs
	Stephan Emmerth, Friedrich Miescher Institute Short Talk: Nuclear Retention of Fission Yeast Dicer Ensures Accurate Gene Expression and Heterochromatin Assembly
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Grays/Torreys Peaks
7:30 - 10:00 PM	Poster Session 3	Grays/Torreys Peaks
Monday, January 18
7:00 - 8:00 AM	Breakfast	Grays/Torreys Peaks
8:00 - 11:00 AM	Small RNAs, Disease and Development	Longs/Quandary Peaks
	Alexander Y. Rudensky, Memorial Sloan-Kettering Cancer Center Role of miRNAs in T-Cell Function

	Beverly L. Davidson, University of Iowa Small RNAs and Huntington’s disease
	Mark P. Boldin, Regulus Therapeutics Short Talk: Myeloproliferative Disease, Autoimmunity and Cancer in Mice with Targeted Deletion of miR-146a Gene
	Deepak Srivastava, Gladstone Institute of Cardiovascular Disease microRNA Regulation of Cardiac Cell Fate, Morphogenesis and Function
	Magnus Blø, University of Bergen Short Talk: Flow Cytometric-Based Functional Selection of RNA Interference Triggers for epi-allelic Analysis
	Tudor Alexandru Fulga, Harvard Medical School Short Talk: Defining microRNA Function with Precise Spatiotemporal Resolution in Intact Organisms
9:20 - 9:40 AM	Coffee Break	Longs Peak Foyer
11:00 AM -	On Own for Lunch and Recreation
4:30 - 5:00 PM	Coffee Available	Longs Peak Foyer
5:00 - 7:00 PM	Small RNAs in Stem Cells and Germ Cells	Longs/Quandary Peaks
	Gene W. Yeo, University of California, San Diego Pinning down microRNA and Targeting in C. elegants and Humans

	Dasaradhi Palakodeti, University of Connecticut Health Center Small RNAs in the Planarian Schmidtea mediterranea
	Colin C. Conine, University of Massachusetts Medical School Short Talk: The Ago-Class Argonautes TZK-1 and TZK-2 Bind 26G-RNAs to Promote Male Fertility in C. elegans
	Alex Bortvin, Carnegie Institution of Washington Short Talk: Maelstrom and Mammalian Meiosis
7:00 - 8:00 PM	Social Hour w/ Lite Bites	Shavano/Torreys Peaks
8:00 - 11:00 PM	Entertainment	Shavano/Torreys Peaks
Tuesday, January 19
	Departure

*Session Chair †Speaker invited, not yet responded.