Web Desc
Dynamics of Cellular Organization
Organizer(s): Jennifer A. Lippincott-Schwartz and David L. Spector
Date: February 22 - 27, 2003
Location: Sagebrush Inn & Suites, Taos, NM, USA
Sponsored in part by The Director's Sponsor Fund
For important information on the coronavirus, please click here
Summary of Meeting:
Some of the most basic features of biological organization are now being unraveled using live cell imaging approaches that analyze the spatio-temporal behavior of molecules in cells. These approaches are providing a dynamic view of protein behavior in cells that integrates information on protein localization, concentration, diffusion and interactions that are indiscernible from protein sequences and in vitro biochemical experiments alone. Precise measurement and analysis of fluorescently labeled proteins in cells has revealed, for example, that nuclear architecture is extremely dynamic and comprised of steady-state assemblies of molecules that undergo cell cycle dependent changes in distributions. It has provided insight into the self-organizing properties of the cytoplasm and membrane lipid/protein assemblies. In addition, it is helping to clarify intracellular pathways of protein trafficking, mechanisms of cell motility, the generation of cell polarity, signaling networks and cell responses to stress. A major challenge now is to understand how these dynamic cellular processes are integrated into hierarchically organized cellular systems that give rise to global cell function. The goal of this meeting is to bring together investigators from different areas of cell biology who utilize live cell experimental and theoretical approaches to discuss fundamental principles governing protein geography and movement within cells and how they subserve broader, integrative cellular functions.
Discounted Abstract Deadline: October 22 2002
Discounted Registration Deadline: December 23 2002
We gratefully acknowledge additional support for this conference from:
Carl Zeiss MicroImaging, Inc.
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

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Program

Saturday, February 22 | 3:00PM - 7:00PM
Registration
Room: Chamisa Lobby


Saturday, February 22 | 6:15PM - 7:15PM
Welcome
Room: Chamisa Lobby


Saturday, February 22 | 7:15PM - 7:30PM
Orientation
Room: Chamisa 1


Saturday, February 22 | 7:30PM - 8:45PM
Opening Session
Room: Chamisa 1

Speaker 1 of 2
Lawrence E. Samelson, National Institutes of Health, USA
Visualizing the Generation of Signaling Complexes at the T Cell Antigen Receptor

Saturday, February 22 | 7:30PM - 8:45PM
Opening Session
Room: Chamisa 1

Speaker 2 of 2
Donald E. Ingber, Wyss Institute for Biologically Inspired Engineering at Harvard, USA
Integration of Structure and Function in Living Cells

Sunday, February 23 | 7:00AM - 8:00AM
Breakfast
Room: Los Vaqueros


Sunday, February 23 | 8:00AM - 11:00AM
Dynamics of Nuclear Organization I
Room: Chamisa 1

Speaker 1 of 5
David L. Spector, Cold Spring Harbor Laboratory, USA
Direct Visualization of Gene Expression in Living Cells

Sunday, February 23 | 8:00AM - 11:00AM
Dynamics of Nuclear Organization I
Room: Chamisa 1

Speaker 2 of 5
Thomas Misteli, NCI, National Institutes of Health, USA
Dynamics of Chromatin Proteins

Sunday, February 23 | 8:00AM - 11:00AM
Dynamics of Nuclear Organization I
Room: Chamisa 1

Speaker 3 of 5
Andrew S. Belmont, University of Illinois at Urbana-Champaign, USA
In vivo Chromatin Organization

Sunday, February 23 | 8:00AM - 11:00AM
Dynamics of Nuclear Organization I
Room: Chamisa 1

Speaker 4 of 5
James G. McNally, National Institutes of Health, USA
Short Talk: Mechanism and Function of Transcription Factor Exchange at a Promoter

Sunday, February 23 | 8:00AM - 11:00AM
Dynamics of Nuclear Organization I
Room: Chamisa 1

Speaker 5 of 5
* Thoru Pederson, University of Massachusetts Medical School, USA
Traffic of Newly Formed Ribosomes in the Nucleus

Sunday, February 23 | 9:20AM - 9:40AM
Coffee Break
Room: Chamisa Lobby


Sunday, February 23 | 11:00AM - 1:00PM
Poster Setup
Room: Chamisa 2


Sunday, February 23 | 1:00PM - 10:00PM
Poster Viewing
Room: Chamisa 2


Sunday, February 23 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Chamisa Lobby


Sunday, February 23 | 5:00PM - 7:00PM
Dynamics of Nuclear Organization II
Room: Chamisa 1

Speaker 1 of 4
* Ian G. Macara, University of Virginia School of Medicine, USA
RAN Dynamics - The Ins and Outs of Nuclear Transport

Sunday, February 23 | 5:00PM - 7:00PM
Dynamics of Nuclear Organization II
Room: Chamisa 1

Speaker 2 of 4
Jan Ellenberg, European Molecular Biology Laboratory, Germany
Dynamics of the Nuclear Envelope and Chromosomes in Mitosis

Sunday, February 23 | 5:00PM - 7:00PM
Dynamics of Nuclear Organization II
Room: Chamisa 1

Speaker 3 of 4
Korie Ellen Handwerger, Cell Press, USA
Short Talk: Steady-State Dynamics of Cajal Body Components in the Xenopus Oocyte Nucleus

Sunday, February 23 | 5:00PM - 7:00PM
Dynamics of Nuclear Organization II
Room: Chamisa 1

Speaker 4 of 4
† Robert DeLotto, Biological Institute, University of Copenhagen, Denmark
Dynamics and Nucleocytoplasmic Shuttling of Drosophila NF-kappa B Protein Dorsal, During Embryonic Development

Sunday, February 23 | 7:00PM - 8:00PM
Social Hour
Room: Chamisa 2


Sunday, February 23 | 8:00PM - 10:00PM
Poster Session 1
Room: Chamisa 2


Monday, February 24 | 7:00AM - 8:00AM
Breakfast
Room: Los Vaqueros


Monday, February 24 | 8:00AM - 11:00AM
Cytoplasmic Organization and Dynamics
Room: Chamisa 1

Speaker 1 of 5
* Gary G. Borisy, Forsyth, USA
Rethinking Microtubule Dynamics

Monday, February 24 | 8:00AM - 11:00AM
Cytoplasmic Organization and Dynamics
Room: Chamisa 1

Speaker 2 of 5
Mark A. McNiven, Mayo Clinic, USA
The Large GTPase Dynamin as a Mediator of Dynamic Actin-Membrane Interactions


Monday, February 24 | 8:00AM - 11:00AM
Cytoplasmic Organization and Dynamics
Room: Chamisa 1

Speaker 3 of 5
John A. Hammer, NHLBI, National Institutes of Health, USA
Molecular Genetic Analysis of Motor: Organelle Interaction

Monday, February 24 | 8:00AM - 11:00AM
Cytoplasmic Organization and Dynamics
Room: Chamisa 1

Speaker 4 of 5
Rachael A. Ream, Stanford University, USA
Short Talk: Deconstruction of Fish Keratoycte Motility

Monday, February 24 | 8:00AM - 11:00AM
Cytoplasmic Organization and Dynamics
Room: Chamisa 1

Speaker 5 of 5
Sandrine Etienne-Manneville, Institut Pasteur, France
Short Talk: Cdc42 Regulates Polarity through the Spatial Regulation of GSK3b and Adenomatous Polyposis coli (APC)

Monday, February 24 | 9:20AM - 9:40AM
Coffee Break
Room: Chamisa Lobby


Monday, February 24 | 11:00AM - 1:00PM
Poster Setup
Room: Chamisa 2


Monday, February 24 | 1:00PM - 10:00PM
Poster Viewing
Room: Chamisa 2


Monday, February 24 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Chamisa Lobby


Monday, February 24 | 5:00PM - 7:00PM
Cell and Organelle Motility
Room: Chamisa 1

Speaker 1 of 3
* Ken Jacobson, University of North Carolina at Chapel Hill, USA
New Methods to Study Cell Migration


Monday, February 24 | 5:00PM - 7:00PM
Cell and Organelle Motility
Room: Chamisa 1

Speaker 2 of 3
Ronald D. Vale, University of California, San Francisco, USA
RNAi Structures, A Kinesis in Living Cells by Microscopy

Monday, February 24 | 5:00PM - 7:00PM
Cell and Organelle Motility
Room: Chamisa 1

Speaker 3 of 3
Kazuhiko Kinosita, Jr., Okazaki National Research Institutes, Japan
Single-Molecule Studies of the Rotary Mechanism of F1-ATPase

Monday, February 24 | 7:00PM - 8:00PM
Social Hour
Room: Chamisa 2


Monday, February 24 | 8:00PM - 10:00PM
Poster Session 2 & 3
Room: Chamisa 2


Tuesday, February 25 | 7:00AM - 8:00AM
Breakfast
Room: Los Vaqueros


Tuesday, February 25 | 8:00AM - 11:00AM
Membrane Trafficking and Cell Polarity
Room: Chamisa 1

Speaker 1 of 5
Jennifer A. Lippincott-Schwartz, Howard Hughes Medical Institute - Janelia Farm, USA
Secretory Protein Transport through the Golgi Apparatus

Tuesday, February 25 | 8:00AM - 11:00AM
Membrane Trafficking and Cell Polarity
Room: Chamisa 1

Speaker 2 of 5
Ari Helenius, ETH Zurich, Switzerland
Caveolar Endocytosis

Tuesday, February 25 | 8:00AM - 11:00AM
Membrane Trafficking and Cell Polarity
Room: Chamisa 1

Speaker 3 of 5
* Kai Simons, Max Planck Institute of Molecular Cell Biology and Genetics, Germany
Lipid Rafts in Membrane Trafficking and Cell Polarity

Tuesday, February 25 | 8:00AM - 11:00AM
Membrane Trafficking and Cell Polarity
Room: Chamisa 1

Speaker 4 of 5
Geri Kreitzer, Weill Medical College of Cornell University, USA
Short Talk: Three-Dimensional Analysis of Post-Golgi Carrier Exocytosis in Epithelial Cells

Tuesday, February 25 | 8:00AM - 11:00AM
Membrane Trafficking and Cell Polarity
Room: Chamisa 1

Speaker 5 of 5
W. James Nelson, Stanford University, USA
Cues for Cellular Asymmetry in Polarized Epithelial Cells

Tuesday, February 25 | 9:20AM - 9:40AM
Coffee Break
Room: Chamisa Lobby


Tuesday, February 25 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Chamisa Lobby


Tuesday, February 25 | 5:00PM - 7:00PM
Intercellular Organization and Invasion
Room: Chamisa 1

Speaker 1 of 3
* Marcos Gonzalez-Gaitan, University of Geneva, Switzerland
Morphogenetic Signaling and the Endocytic Pathway

Tuesday, February 25 | 5:00PM - 7:00PM
Intercellular Organization and Invasion
Room: Chamisa 1

Speaker 2 of 3
Thomas Lecuit, Universite of Marseille, France
Polarized Membrane Growth in Epithelial Morphogenesis

Tuesday, February 25 | 5:00PM - 7:00PM
Intercellular Organization and Invasion
Room: Chamisa 1

Speaker 3 of 3
† Ajay B. Chitnis, National Institutes of Health, USA
Mind Bomb is a Ubiquitin Ligase that is Essential for Efficient Activation of Notch Signaling by Delta

Tuesday, February 25 | 7:00PM - 8:00PM
Social Hour
Room: Chamisa 2


Tuesday, February 25 | 8:00PM - 10:00PM
Workshop
Room: Chamisa 2

Speaker 1 of 7
* Peter N. Devreotes, Johns Hopkins University, USA

Tuesday, February 25 | 8:00PM - 10:00PM
Workshop
Room: Chamisa 2

Speaker 2 of 7
Robert D. Phair, Integrative BioInformatics Inc., USA
Database Support for Managing and Testing Complex Molecular Cell Biological Hypotheses

Tuesday, February 25 | 8:00PM - 10:00PM
Workshop
Room: Chamisa 2

Speaker 3 of 7
Ivan Raska, Institute of Experimental Medicine Academy of Sciences of Czech Republic, Czech Republic
Analysis of Replication Sites in HeLa Cells during the Cell Cycle

Tuesday, February 25 | 8:00PM - 10:00PM
Workshop
Room: Chamisa 2

Speaker 4 of 7
Paula Ann Bubulya, Wright State University, USA
PRE-mRNA Splicing Factors Initially Localize to Active NORs Upon Entry into Daughter Nuclei

Tuesday, February 25 | 8:00PM - 10:00PM
Workshop
Room: Chamisa 2

Speaker 5 of 7
Jomon Joseph, India
Regulation and Function of Ran in the Cell Cycle

Tuesday, February 25 | 8:00PM - 10:00PM
Workshop
Room: Chamisa 2

Speaker 6 of 7
Yves P. Barral, ETH Zürich, Switzerland
How Microtubues Find the Pole during Spindle Orientation in Yeast

Tuesday, February 25 | 8:00PM - 10:00PM
Workshop
Room: Chamisa 2

Speaker 7 of 7
Erik L. Snapp, Albert Einstein College of Medicine, USA
Self-Organization of Stacked Lamellar Membranes from Tubular ER Membranes in Living Cells

Wednesday, February 26 | 7:00AM - 8:00AM
Breakfast
Room: Los Vaqueros


Wednesday, February 26 | 8:00AM - 11:00AM
Signaling Networks: Linking Cytoplasm and Membrane with the
Outside World

Room: Chamisa 1

Speaker 1 of 5
Tobias Meyer, Stanford University Medical Center, USA
Spatial and Temporal Control of Signaling Protein Localization

Wednesday, February 26 | 8:00AM - 11:00AM
Signaling Networks: Linking Cytoplasm and Membrane with the
Outside World

Room: Chamisa 1

Speaker 2 of 5
Peter N. Devreotes, Johns Hopkins University, USA
How Cells Sense Chemoattractant Gradients

Wednesday, February 26 | 8:00AM - 11:00AM
Signaling Networks: Linking Cytoplasm and Membrane with the
Outside World

Room: Chamisa 1

Speaker 3 of 5
Michael L. Dustin, University of Oxford, UK
The Immunological Synapse: A Swiss Army Knife for Signaling

Wednesday, February 26 | 8:00AM - 11:00AM
Signaling Networks: Linking Cytoplasm and Membrane with the
Outside World

Room: Chamisa 1

Speaker 4 of 5
* Benjamin Geiger, Weizmann Institute of Science, Israel
Cell Adhesion: The First Few Moments

Wednesday, February 26 | 8:00AM - 11:00AM
Signaling Networks: Linking Cytoplasm and Membrane with the
Outside World

Room: Chamisa 1

Speaker 5 of 5
Mark R. Philips, New York University Cancer Institute, USA
Short Talk: Compartment-Specific Regulation of Ras: PLCgamma Activates Ras on Golgi via RasGRP1

Wednesday, February 26 | 9:20AM - 9:40AM
Coffee Break
Room: Chamisa Lobby


Wednesday, February 26 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Chamisa Lobby


Wednesday, February 26 | 5:00PM - 7:00PM
Cell Responses: Tension, Tearing and Resealing
Room: Chamisa 1

Speaker 1 of 4
* Daniel P. Kiehart, Duke University, USA
Modeling Morphogenesis: Laser Microsurgery, Mechanical Jump Experiments and Analysis of Mutants Reveal the Origin of Forces for Dorsal Closure

Wednesday, February 26 | 5:00PM - 7:00PM
Cell Responses: Tension, Tearing and Resealing
Room: Chamisa 1

Speaker 2 of 4
Paul L. McNeil, Georgia Regents University, USA
Coping with the Inevitable: How Cells Repair a Torn Surface Membrane

Wednesday, February 26 | 5:00PM - 7:00PM
Cell Responses: Tension, Tearing and Resealing
Room: Chamisa 1

Speaker 3 of 4
Jean-Baptiste Manneville, National Institute for Medical Research, MRC, UK
Short Talk: Dynamics of Cdc42 in Migrating Astrocytes Visualized by Total Internal Reflection Fluorescence Microscopy

Wednesday, February 26 | 5:00PM - 7:00PM
Cell Responses: Tension, Tearing and Resealing
Room: Chamisa 1

Speaker 4 of 4
Jeffrey E. Segall, Albert Einstein College of Medicine, USA
Tumor Cell Metastasis: In vivo and in vitro studies

Wednesday, February 26 | 7:00PM - 8:00PM
Social Hour
Room: Chamisa 2


Wednesday, February 26 | 8:00PM - 11:00PM
Entertainment
Room: Chamisa 2


Wednesday, February 26 | 8:00PM - 11:00PM
Cash Bar
Room: Chamisa 2


Thursday, February 27 | 10:21AM - 10:21AM
Departure


*Session Chair
†Invited, not yet responded.