Web Desc
Proteomics: Technologies and Applications
Organizer(s): Ruedi Aebersold and Stanley Fields
Date: March 25 - 30, 2003
Location: Keystone Resort, Keystone, CO, USA
Supported by Keystone Symposia
Summary of Meeting:
The completion of the genomic sequences of a number of prokaryotic and eukaryotic species has catalyzed new research approaches to study the structure, function, and control of biological processes. They are characterized by the systematic and, in many cases, quantitative analysis of all the molecules of a particular type expressed by a cell or tissue. The systematic analysis of proteins has been termed 'proteomics.' In an initial phase, most of the proteomics efforts were focused on large-scale protein identification. More recently, the objectives and technologies of proteomics have diversified and expanded. Current proteomics research attempts to systematically and, where applicable, quantitatively determine the many properties of proteins that determine their biological function. These include protein abundance, state of modification, specific activity, interaction with other biomolecules, half-life, subcellular location, structure, and more. Significant current challenges include the development of suitable technologies to determine these properties on a proteome-wide scale, the interpretation of the large amounts of data obtained, the development of computational approaches to infer biological function, and the integration of different types of data into a coherent model describing a biological process. The goal of the meeting is to bring together leading experts from the different areas of proteomics research to discuss emerging technologies and their applications to biological and clinical research.
Discounted Abstract Deadline: November 25 2002
Discounted Registration Deadline: January 27 2003
We gratefully acknowledge the generous grant for this conference provided by:

National Institute of Environmental Health Sciences (NIEHS)
Grant No. 1 R13 ES012094-01

National Nuclear Security Administration (DOE/NNSA)
Grant No. DE-FO03-03ER63517
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Tuesday, March 25 | 3:00PM - 7:00PM
Registration
Room: Shavano Peak Foyer


Tuesday, March 25 | 6:15PM - 7:15PM
Welcome
Room: Shavano Peak Foyer


Tuesday, March 25 | 7:15PM - 7:30PM
Orientation
Room: Shavano Peak


Tuesday, March 25 | 7:30PM - 8:30PM
Keynote Address
Room: Shavano Peak

Speaker 1 of 2
* Rudolf H. Aebersold, Institute of Molecular Systems Biology, ETH Zürich, Switzerland

Tuesday, March 25 | 7:30PM - 8:30PM
Keynote Address
Room: Shavano Peak

Speaker 2 of 2
Tony Pawson, Samuel Lunenfeld Research Institute, Canada
Modular Protein Interactions and Signaling Networks

Wednesday, March 26 | 7:00AM - 8:00AM
Breakfast
Room: Torreys Peak


Wednesday, March 26 | 8:00AM - 11:00AM
Identification and Quantification of Proteins on a Proteome-
Wide Scale

Room: Shavano Peak

Speaker 1 of 5
* Pavel A. Pevzner, University of California, San Diego, USA

Wednesday, March 26 | 8:00AM - 11:00AM
Identification and Quantification of Proteins on a Proteome-
Wide Scale

Room: Shavano Peak

Speaker 2 of 5
Rudolf H. Aebersold, Institute of Molecular Systems Biology, ETH Zürich, Switzerland
Toward a High Throughput Quantitative Proteomics Platform

Wednesday, March 26 | 8:00AM - 11:00AM
Identification and Quantification of Proteins on a Proteome-
Wide Scale

Room: Shavano Peak

Speaker 3 of 5
Marvin Vestal, Applied Biosystems, USA
Trends in Mass Spectrometry Instrumentation for Proteomics

Wednesday, March 26 | 8:00AM - 11:00AM
Identification and Quantification of Proteins on a Proteome-
Wide Scale

Room: Shavano Peak

Speaker 4 of 5
John R. Yates III, The Scripps Research Institute, USA
Proteomic View of Malaria Parasite Life Cycle

Wednesday, March 26 | 8:00AM - 11:00AM
Identification and Quantification of Proteins on a Proteome-
Wide Scale

Room: Shavano Peak

Speaker 5 of 5
Scott L. Diamond, University of Pennsylvania, USA
Short Talk: Printing Chemical Libraries on Microarray for Nanoliter Fluid Phase Reactions

Wednesday, March 26 | 9:20AM - 9:40AM
Coffee Break
Room: Shavano Peak Foyer


Wednesday, March 26 | 11:00AM - 1:00PM
Poster Setup
Room: Torreys Peak


Wednesday, March 26 | 1:00PM - 10:00PM
Poster Viewing
Room: Torreys Peak


Wednesday, March 26 | 4:00PM - 4:30PM
Special Lecture
Room: Shavano Peak

Speaker 1 of 1
Gregory Milman, National Institutes of Health, USA
Over $1 Billion in Biodefense Research Opportunities

Wednesday, March 26 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Shavano Peak Foyer


Wednesday, March 26 | 5:00PM - 7:00PM
Analysis, Interpretation and Management of Proteomics Data
Room: Shavano Peak

Speaker 1 of 4
* John R. Yates III, The Scripps Research Institute, USA

Wednesday, March 26 | 5:00PM - 7:00PM
Analysis, Interpretation and Management of Proteomics Data
Room: Shavano Peak

Speaker 2 of 4
Benno Schwikowski, Institute for Systems Biology, USA
Computational Tools and Challenges for Integrating Proteomic Data

Wednesday, March 26 | 5:00PM - 7:00PM
Analysis, Interpretation and Management of Proteomics Data
Room: Shavano Peak

Speaker 3 of 4
Pavel A. Pevzner, University of California, San Diego, USA
New Algorithmic Approaches for the Analysis of Peptide Mass Spectral Data

Wednesday, March 26 | 5:00PM - 7:00PM
Analysis, Interpretation and Management of Proteomics Data
Room: Shavano Peak

Speaker 4 of 4
Bruno Domon, Celera Genomics, Inc., USA
Generation and Analysis of Data for Target Discovery

Wednesday, March 26 | 7:00PM - 8:00PM
Social Hour
Room: Torreys Peak


Wednesday, March 26 | 8:00PM - 10:00PM
Poster Session 1: Identification and Quantification of Prote
ins/Management of Proteomics Data
Room: Torreys Peak


Thursday, March 27 | 7:00AM - 8:00AM
Breakfast
Room: Torreys Peak


Thursday, March 27 | 8:00AM - 11:30AM
Protein Interactions and Protein Linkage Maps
Room: Shavano Peak

Speaker 1 of 7
* Elizabeth J. Grayhack, University of Rochester School of Medicine, USA

Thursday, March 27 | 8:00AM - 11:30AM
Protein Interactions and Protein Linkage Maps
Room: Shavano Peak

Speaker 2 of 7
Joachim Frank, Columbia University, USA
Single-Particle Reconstruction of Molecular Machines Using Cryo-Electron Microscopy

Thursday, March 27 | 8:00AM - 11:30AM
Protein Interactions and Protein Linkage Maps
Room: Shavano Peak

Speaker 3 of 7
Stanley Fields, University of Washington, USA
Genetic and Genomic Approaches to Protein Interaction

Thursday, March 27 | 8:00AM - 11:30AM
Protein Interactions and Protein Linkage Maps
Room: Shavano Peak

Speaker 4 of 7
Marc Vidal, Dana-Farber Cancer Institute, USA
Toward a Proteome Atlas for C. elegans

Thursday, March 27 | 8:00AM - 11:30AM
Protein Interactions and Protein Linkage Maps
Room: Shavano Peak

Speaker 5 of 7
Bertrand Séraphin, Universite Paris 6, France
Large Scale Protein Linkage Maps via Purification of Protein Complexes and Mass Spectrometric Analysis

Thursday, March 27 | 8:00AM - 11:30AM
Protein Interactions and Protein Linkage Maps
Room: Shavano Peak

Speaker 6 of 7
Amy L. Hitchcock, Dana Farber Cancer Institute, USA
Short Talk: Identification of Ubiquitinated ER Proteins

Thursday, March 27 | 8:00AM - 11:30AM
Protein Interactions and Protein Linkage Maps
Room: Shavano Peak

Speaker 7 of 7
Ole N. Jensen, University of Southern Denmark, Denmark
Short Talk: Proteomic Strategy for Identification of GPI-Anchored Proteins in H. sapiens and A. thaliana Cell Membranes

Thursday, March 27 | 9:20AM - 9:40AM
Coffee Break
Room: Shavano Peak Foyer


Thursday, March 27 | 11:30AM - 1:00PM
Poster Setup
Room: Torreys Peak


Thursday, March 27 | 1:00PM - 10:00PM
Poster Viewing
Room: Torreys Peak


Thursday, March 27 | 4:00PM - 4:30PM
Special Lecture
Room: Shavano Peak

Speaker 1 of 1
Gregory Milman, National Institutes of Health, USA
Whom to Contact at NIH and When?

Thursday, March 27 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Shavano Peak Foyer


Thursday, March 27 | 5:00PM - 7:00PM
In vivo Analysis
Room: Shavano Peak

Speaker 1 of 3
* Stanley Fields, University of Washington, USA

Thursday, March 27 | 5:00PM - 7:00PM
In vivo Analysis
Room: Shavano Peak

Speaker 2 of 3
Stephen W. Michnick, Université de Montréal, Canada
Mapping and Visualization of Biochemical Networks in Living Cells

Thursday, March 27 | 5:00PM - 7:00PM
In vivo Analysis
Room: Shavano Peak

Speaker 3 of 3
Andrew Fraser, Wellcome Trust Sanger Institute, UK
Functional Analysis of the C. elegans Genome by Rna-Mediated Interference

Thursday, March 27 | 7:00PM - 8:00PM
Social Hour
Room: Torreys Peak


Thursday, March 27 | 8:00PM - 10:00PM
Poster Session 2: In vivo Approaches/Analysis of Protein Act
ivities
Room: Torreys Peak


Friday, March 28 | 7:00AM - 8:00AM
Breakfast
Room: Torreys Peak


Friday, March 28 | 8:00AM - 11:30AM
Systematic Analysis of Protein Activities and Protein Arrays
Room: Shavano Peak

Speaker 1 of 6
* Susan S. Taylor, University of California, San Diego, USA

Friday, March 28 | 8:00AM - 11:30AM
Systematic Analysis of Protein Activities and Protein Arrays
Room: Shavano Peak

Speaker 2 of 6
Michael P. Snyder, Stanford University School of Medicine, USA
Global Analysis of the Yeast Proteome

Friday, March 28 | 8:00AM - 11:30AM
Systematic Analysis of Protein Activities and Protein Arrays
Room: Shavano Peak

Speaker 3 of 6
Dolores J. Cahill, Pfizer Inc., Ireland
Protein Arrays: Generation and Recent Applications

Friday, March 28 | 8:00AM - 11:30AM
Systematic Analysis of Protein Activities and Protein Arrays
Room: Shavano Peak

Speaker 4 of 6
Gavin MacBeath, Abpro, USA
Protein Microarrays: From Functional Genomics to Protein Profiling

Friday, March 28 | 8:00AM - 11:30AM
Systematic Analysis of Protein Activities and Protein Arrays
Room: Shavano Peak

Speaker 5 of 6
Larry Gold, SomaLogic Inc., USA
Aptamer Arrays and Proteomics

Friday, March 28 | 8:00AM - 11:30AM
Systematic Analysis of Protein Activities and Protein Arrays
Room: Shavano Peak

Speaker 6 of 6
Alissa Marie Resch, University of California, Los Angeles, USA
Short Talk: Switching Protein Interaction Networks by Alternative Splicing of Protein Interaction Domains

Friday, March 28 | 9:20AM - 9:40AM
Coffee Break
Room: Shavano Peak Foyer


Friday, March 28 | 11:30AM - 1:00PM
Poster Setup
Room: Torreys Peak


Friday, March 28 | 1:00PM - 10:00PM
Poster Viewing
Room: Torreys Peak


Friday, March 28 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Shavano Peak Foyer


Friday, March 28 | 5:00PM - 7:00PM
Structural Proteomics
Room: Shavano Peak

Speaker 1 of 4
* Michael P. Snyder, Stanford University School of Medicine, USA

Friday, March 28 | 5:00PM - 7:00PM
Structural Proteomics
Room: Shavano Peak

Speaker 2 of 4
David Baker, University of Washington, USA
Prediction and Design of Protein Structures and Protein-Protein
Interactions


Friday, March 28 | 5:00PM - 7:00PM
Structural Proteomics
Room: Shavano Peak

Speaker 3 of 4
Andrej Sali, University of California, San Francisco, USA
Modeling the Structure of Proteins and Macromolecular Assemblies

Friday, March 28 | 5:00PM - 7:00PM
Structural Proteomics
Room: Shavano Peak

Speaker 4 of 4
Susan S. Taylor, University of California, San Diego, USA
Dynamic Integration of Signaling by PKA

Friday, March 28 | 7:00PM - 8:00PM
Social Hour
Room: Torreys Peak


Friday, March 28 | 8:00PM - 10:00PM
Poster Session 3: Structural Proteomics/Control of Protein A
bundance and Activity/Computational Methods
Room: Torreys Peak


Saturday, March 29 | 7:00AM - 8:00AM
Breakfast
Room: Torreys Peak


Saturday, March 29 | 8:00AM - 11:30AM
Control and Detection of Protein Abundance and Activity
Room: Shavano Peak

Speaker 1 of 7
* Dolores J. Cahill, Pfizer Inc., Ireland

Saturday, March 29 | 8:00AM - 11:30AM
Control and Detection of Protein Abundance and Activity
Room: Shavano Peak

Speaker 2 of 7
David R. Morris, University of Washington, USA
Translating the Transcriptome: High Throughput Analysis of mRNA Translation

Saturday, March 29 | 8:00AM - 11:30AM
Control and Detection of Protein Abundance and Activity
Room: Shavano Peak

Speaker 3 of 7
Brian T. Chait, Rockefeller University, USA
Probes of Protein Interaction and Phosphorylation

Saturday, March 29 | 8:00AM - 11:30AM
Control and Detection of Protein Abundance and Activity
Room: Shavano Peak

Speaker 4 of 7
Benjamin F. Cravatt III, The Scripps Research Institute, USA
Activity-Based Protein Profiling: Chemical Approaches for Functional Proteomics

Saturday, March 29 | 8:00AM - 11:30AM
Control and Detection of Protein Abundance and Activity
Room: Shavano Peak

Speaker 5 of 7
Elizabeth J. Grayhack, University of Rochester School of Medicine, USA
Functional Proteomics

Saturday, March 29 | 8:00AM - 11:30AM
Control and Detection of Protein Abundance and Activity
Room: Shavano Peak

Speaker 6 of 7
Joshua E. Elias, Harvard Medical School, USA
Short Talk: Predicting MS/MS Peptide Fragmentation Patterns: Beyond SEQUEST

Saturday, March 29 | 8:00AM - 11:30AM
Control and Detection of Protein Abundance and Activity
Room: Shavano Peak

Speaker 7 of 7
† Lisa M. Gangi, National Cancer Institute, National Institutes of Health, USA
Short Talk: Development of Antibody Microarrays for Detection and Quantitation of Specific Proteins

Saturday, March 29 | 9:20AM - 9:40AM
Coffee Break
Room: Shavano Peak Foyer


Saturday, March 29 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Shavano Peak Foyer


Saturday, March 29 | 5:00PM - 7:00PM
Methods to Explore Function
Room: Shavano Peak

Speaker 1 of 4
* Brian T. Chait, Rockefeller University, USA

Saturday, March 29 | 5:00PM - 7:00PM
Methods to Explore Function
Room: Shavano Peak

Speaker 2 of 4
Tom W. Muir, Princeton University, USA
From Protein Semisynthesis to Chemical Genetics: The
Chemical Biology of Protein Splicing


Saturday, March 29 | 5:00PM - 7:00PM
Methods to Explore Function
Room: Shavano Peak

Speaker 3 of 4
Edward M. Marcotte, University of Texas at Austin, USA
Global Analysis of Protein Pathways, Systems and Interactions


Saturday, March 29 | 5:00PM - 7:00PM
Methods to Explore Function
Room: Shavano Peak

Speaker 4 of 4
Peer Bork, European Molecular Biology Laboratory, Germany
Comparative Analysis of Protein Interaction Networks

Saturday, March 29 | 7:00PM - 8:00PM
Social Hour
Room: Torreys Peak


Saturday, March 29 | 8:00PM - 11:00PM
Cash Bar
Room: Torreys Peak


Saturday, March 29 | 8:00PM - 11:00PM
Entertainment
Room: Torreys Peak


Sunday, March 30 | 10:26AM - 10:26AM
Departure


*Session Chair.