Web Desc
siRNAs and miRNAs
Organizer(s): Thomas Tuschl and Victor Ambros
Date: April 14 - 19, 2004
Location: Keystone Resort, Keystone, CO, USA
Sponsored by Alnylam Pharmaceuticals, Inc. and Merck Research Laboratories
For important information on the coronavirus, please click here
Summary of Meeting:
Short double-stranded RNA molecules are important sequence-specific posttranscriptional regulators of gene expression. Two classes of such RNA molecules have been identified: small interfering RNAs (siRNAs) and microRNAs (miRNAs). While siRNAs are generally complementary to mRNAs and mediate mRNA degradation, most miRNAs are only partially complementary and are believed to act predominantly as translational regulators. siRNAs and microRNA-like siRNA precursors have become important tools for studying gene function in mammalian cells and organisms. miRNAs represent an extensive class of evolutionary conserved noncoding RNAs of about 22 nucleotide in length that are thought to regulate gene expression in metazoans. The first miRNAs to be identified were the products of the lin-4 and let-7 genes of Caenorhabditis elegans. The miRNA and RNAi pathways are fundamentally related as members of the Dicer and the Argonaute protein families are involved in both of these RNA-mediated silencing processes. The aim of this meeting is to report on progress in understanding the molecular mechanisms underlying siRNA/miRNA function, as well as the technological adaptation of these cellular mechanisms to genome-wide analysis of gene function in mammalian systems and as therapeutic agents.
Discounted Abstract Deadline: December 16 2003
Discounted Registration Deadline: February 17 2004
We gratefully acknowledge additional support for this conference from:
Alnylam Pharmaceuticals, Inc.Ambion, Inc.
Cell Signaling Technology, Inc.
Cenix BioScience GmbH
GenoFunction, Inc.
Isis Pharmaceuticals, Inc.Merck Research LaboratoriesMirus Bio LLCPromega CorporationSIRNA TherapeuticsThermo Fisher Scientific, Inc.
We gratefully acknowledge the generous grant for this conference provided by:

National Cancer Institute (NCI)
Grant No. 1R13CA105911-01
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Wednesday, April 14 | 3:00PM - 7:00PM
Registration
Room: Longs Peak Foyer


Wednesday, April 14 | 6:15PM - 7:15PM
Refreshments
Room: Longs Peak Foyer


Wednesday, April 14 | 7:15PM - 7:30PM
Orientation
Room: Grays/Longs


Wednesday, April 14 | 7:30PM - 9:30PM
Keynote Session
Room: Grays/Longs

Speaker 1 of 3
* David C. Baulcombe, University of Cambridge, UK

Wednesday, April 14 | 7:30PM - 9:30PM
Keynote Session
Room: Grays/Longs

Speaker 2 of 3
Ronald H. Plasterk, Ministry of Education, Culture and Science, Netherlands
RNAi as the Immune System of the C. elegans Genome

Wednesday, April 14 | 7:30PM - 9:30PM
Keynote Session
Room: Grays/Longs

Speaker 3 of 3
Andrew Fire, Stanford University, USA
RNA-Based Immunity

Thursday, April 15 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Thursday, April 15 | 8:00AM - 11:15AM
RNomics of miRNAs and Other Non-Coding RNAs. Session Sponso
red in part by ISIS Pharmaceuticals, Inc.

Room: Grays/Longs

Speaker 1 of 7
* Brenda L. Bass, University of Utah, USA

Thursday, April 15 | 8:00AM - 11:15AM
RNomics of miRNAs and Other Non-Coding RNAs. Session Sponso
red in part by ISIS Pharmaceuticals, Inc.

Room: Grays/Longs

Speaker 2 of 7
Alexander Hüttenhofer, Universität Innsbruck, Austria
Experimental RNomics: Identification of Small Non-Messenger RNAs in Model Organisms

Thursday, April 15 | 8:00AM - 11:15AM
RNomics of miRNAs and Other Non-Coding RNAs. Session Sponso
red in part by ISIS Pharmaceuticals, Inc.

Room: Grays/Longs

Speaker 3 of 7
David P. Bartel, Massachusetts Institute of Technology and Whitehead Institute, USA
MicroRNAs in Animals and Plants

Thursday, April 15 | 8:00AM - 11:15AM
RNomics of miRNAs and Other Non-Coding RNAs. Session Sponso
red in part by ISIS Pharmaceuticals, Inc.

Room: Grays/Longs

Speaker 4 of 7
Mark A. Kay, Stanford University, USA
RNAi Therapeutics in Animal Models of Human Hepatitis Infection

Thursday, April 15 | 8:00AM - 11:15AM
RNomics of miRNAs and Other Non-Coding RNAs. Session Sponso
red in part by ISIS Pharmaceuticals, Inc.

Room: Grays/Longs

Speaker 5 of 7
Oliver Hobert, Columbia University, USA
Short Talk: Two Asymmetrically Distributed and Sequentially Acting microRNAs Control Chemosensory Laterality in C.elegans

Thursday, April 15 | 8:00AM - 11:15AM
RNomics of miRNAs and Other Non-Coding RNAs. Session Sponso
red in part by ISIS Pharmaceuticals, Inc.

Room: Grays/Longs

Speaker 6 of 7
James C. Carrington, Donald Danforth Plant Science Center, USA
Small RNAs, Development and Viruses in Plants

Thursday, April 15 | 8:00AM - 11:15AM
RNomics of miRNAs and Other Non-Coding RNAs. Session Sponso
red in part by ISIS Pharmaceuticals, Inc.

Room: Grays/Longs

Speaker 7 of 7
André Verdel, Harvard Medical School, USA
Short Talk: RNAi-Mediated Targeting of Heterochromatin by the RITS Complex

Thursday, April 15 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Thursday, April 15 | 11:15AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Thursday, April 15 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Thursday, April 15 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Longs Peak Foyer


Thursday, April 15 | 5:00PM - 7:00PM
miRNAs in Development and Disease, microRNA Targets
Room: Grays/Longs

Speaker 1 of 5
* David P. Bartel, Massachusetts Institute of Technology and Whitehead Institute, USA

Thursday, April 15 | 5:00PM - 7:00PM
miRNAs in Development and Disease, microRNA Targets
Room: Grays/Longs

Speaker 2 of 5
Gary B. Ruvkun, Massachusetts General Hospital, Harvard Medical School, USA
Enhanced RNAi Mutants of C. elegans

Thursday, April 15 | 5:00PM - 7:00PM
miRNAs in Development and Disease, microRNA Targets
Room: Grays/Longs

Speaker 3 of 5
Frank J. Slack, BIDMC Cancer Center/Harvard Medical School, USA
Temporally Expressed miRNAs and the Control of Developmental Timing

Thursday, April 15 | 5:00PM - 7:00PM
miRNAs in Development and Disease, microRNA Targets
Room: Grays/Longs

Speaker 4 of 5
Ann Rougvie, USA
MicroRNAs and the Control of Developmental Time

Thursday, April 15 | 5:00PM - 7:00PM
miRNAs in Development and Disease, microRNA Targets
Room: Grays/Longs

Speaker 5 of 5
Javier F. Palatnik, Institute of Molecular and Cell Biology of Rosario, Argentina
Short Talk: Control of Leaf Morphogenesis by microRNAs

Thursday, April 15 | 7:00PM - 8:00PM
Social Hour
Room: Shavano/Torreys


Thursday, April 15 | 7:30PM - 10:00PM
Poster Session 1: miRNAs and Other Non-Coding RNAs
Room: Shavano/Torreys


Friday, April 16 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Friday, April 16 | 8:00AM - 11:15AM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 1. Session Sponsored by Dharmacon, Inc.

Room: Grays/Longs

Speaker 1 of 7
* Frank J. Slack, BIDMC Cancer Center/Harvard Medical School, USA

Friday, April 16 | 8:00AM - 11:15AM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 1. Session Sponsored by Dharmacon, Inc.

Room: Grays/Longs

Speaker 2 of 7
Craig C. Mello, University of Massachusetts Medical School, USA
RNAi and Development in C. elegans

Friday, April 16 | 8:00AM - 11:15AM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 1. Session Sponsored by Dharmacon, Inc.

Room: Grays/Longs

Speaker 3 of 7
Witold Filipowicz, Friedrich Miescher Institute for Biomedical Research, Switzerland
Biochemical Analysis of Dicer in Mammalian Cells

Friday, April 16 | 8:00AM - 11:15AM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 1. Session Sponsored by Dharmacon, Inc.

Room: Grays/Longs

Speaker 4 of 7
Thomas Tuschl, HHMI/Rockefeller University, USA
Transcriptional and Posttranscriptional Gene Regulation by dsRNA-Derived Small RNAs

Friday, April 16 | 8:00AM - 11:15AM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 1. Session Sponsored by Dharmacon, Inc.

Room: Grays/Longs

Speaker 5 of 7
Brenda L. Bass, University of Utah, USA
The Intersection of RNA Editing and RNA Interference

Friday, April 16 | 8:00AM - 11:15AM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 1. Session Sponsored by Dharmacon, Inc.

Room: Grays/Longs

Speaker 6 of 7
Young Sik Lee, Northwestern University, USA
Short Talk: Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways

Friday, April 16 | 8:00AM - 11:15AM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 1. Session Sponsored by Dharmacon, Inc.

Room: Grays/Longs

Speaker 7 of 7
Qinghua Liu, University of Texas Southwestern Medical Center, USA
Short Talk: R2D2 Bridges the Initiation and Effector Steps of the Drosophila RNAi Pathway

Friday, April 16 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Friday, April 16 | 11:15AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Friday, April 16 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Friday, April 16 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Longs Peak Foyer


Friday, April 16 | 5:00PM - 7:00PM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 2. Session Sponsored in part by Promega Corporation.

Room: Grays/Longs

Speaker 1 of 5
* Witold Filipowicz, Friedrich Miescher Institute for Biomedical Research, Switzerland

Friday, April 16 | 5:00PM - 7:00PM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 2. Session Sponsored in part by Promega Corporation.

Room: Grays/Longs

Speaker 2 of 5
Phillip D. Zamore, University of Massachusetts Medical School, USA
RNA Interference: Combining Genetics and Biochemistry to Understand Biology and Mechanism

Friday, April 16 | 5:00PM - 7:00PM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 2. Session Sponsored in part by Promega Corporation.

Room: Grays/Longs

Speaker 3 of 5
V. Narry Kim, Institute for Basic Science, South Korea
microRNA Biogenesis

Friday, April 16 | 5:00PM - 7:00PM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 2. Session Sponsored in part by Promega Corporation.

Room: Grays/Longs

Speaker 4 of 5
Anastasia Khvorova, University of Massachusetts Medical School, USA
Mechanistic Aspects of Rational siRNA Design

Friday, April 16 | 5:00PM - 7:00PM
Molecular Mechanisms of RNAi and miRNA Biogenesis and Functi
on - 2. Session Sponsored in part by Promega Corporation.

Room: Grays/Longs

Speaker 5 of 5
Keqiong Ye, Memorial Sloan-Kettering Cancer Center, USA
Short Talk: Crystallographic Structures of Two Protein-siRNA Complexes in the RNA Interference Pathway

Friday, April 16 | 7:00PM - 8:00PM
Social Hour
Room: Shavano/Torreys


Friday, April 16 | 7:30PM - 10:00PM
Poster Session 2: Molecular Mechanisms of siRNA and miRNA Bi
ogenesis and Function
Room: Shavano/Torreys


Saturday, April 17 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Saturday, April 17 | 8:00AM - 11:00AM
Large-Scale RNAi Screens in Cultured Mammalian Cells
Room: Grays/Longs

Speaker 1 of 6
* Gary B. Ruvkun, Massachusetts General Hospital, Harvard Medical School, USA

Saturday, April 17 | 8:00AM - 11:00AM
Large-Scale RNAi Screens in Cultured Mammalian Cells
Room: Grays/Longs

Speaker 2 of 6
Gregory J. Hannon, University of Cambridge, UK
RNAi Mechanism and Application

Saturday, April 17 | 8:00AM - 11:00AM
Large-Scale RNAi Screens in Cultured Mammalian Cells
Room: Grays/Longs

Speaker 3 of 6
René Bernards, Netherlands Cancer Institute, Netherlands
High-Throughput Loss-of-Function Genetic Screens in Mammalian Cells Using RNA Interference

Saturday, April 17 | 8:00AM - 11:00AM
Large-Scale RNAi Screens in Cultured Mammalian Cells
Room: Grays/Longs

Speaker 4 of 6
Peter S. Linsley, AVI BioPharma, USA
What Expression Profiles Tell Us About siRNAs

Saturday, April 17 | 8:00AM - 11:00AM
Large-Scale RNAi Screens in Cultured Mammalian Cells
Room: Grays/Longs

Speaker 5 of 6
Iwao Nozawa, TagCyx Biotechnologies, Japan
Enzymatic Production of siRNA Expression Library

Saturday, April 17 | 8:00AM - 11:00AM
Large-Scale RNAi Screens in Cultured Mammalian Cells
Room: Grays/Longs

Speaker 6 of 6
Mark R. Edbrooke, GlaxoSmithKline, UK
Short Talk: The Use of RNAi in the Drug Discovery Process

Saturday, April 17 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Saturday, April 17 | 11:00AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Saturday, April 17 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Saturday, April 17 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Longs Peak Foyer


Saturday, April 17 | 5:00PM - 7:00PM
Large-Scale RNAi Screens in Non-Mammalian Organisms Session
Sponsored in part by Cenix BioScience and Mirus Corporation

Room: Grays/Longs

Speaker 1 of 4
* James C. Carrington, Donald Danforth Plant Science Center, USA

Saturday, April 17 | 5:00PM - 7:00PM
Large-Scale RNAi Screens in Non-Mammalian Organisms Session
Sponsored in part by Cenix BioScience and Mirus Corporation

Room: Grays/Longs

Speaker 2 of 4
Christophe J. Echeverri, Cenix BioScience GmbH, Germany
Genome-Wide RNAi Screens from C. elegans and Drosophila to Human Cells and Back


Saturday, April 17 | 5:00PM - 7:00PM
Large-Scale RNAi Screens in Non-Mammalian Organisms Session
Sponsored in part by Cenix BioScience and Mirus Corporation

Room: Grays/Longs

Speaker 3 of 4
David C. Baulcombe, University of Cambridge, UK
Mechanisms and Applications of RNA Silencing in Plants

Saturday, April 17 | 5:00PM - 7:00PM
Large-Scale RNAi Screens in Non-Mammalian Organisms Session
Sponsored in part by Cenix BioScience and Mirus Corporation

Room: Grays/Longs

Speaker 4 of 4
Michael Metzlaff, Bayer BioScience N.V., Belgium
Satellite RNA Virus-Based Gene Silencing Screens in Plants

Saturday, April 17 | 7:00PM - 8:00PM
Social Hour
Room: Shavano/Torreys


Saturday, April 17 | 7:30PM - 10:00PM
Poster Session 3: Phenotypic Analysis and Screening Using ds
RNA and RNA-Based Therapeutics
Room: Shavano/Torreys


Sunday, April 18 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Sunday, April 18 | 8:00AM - 11:00AM
Development of Gene-Specific Double-Stranded RNA Drugs. Ses
sion Sponsored by Sirna Therapeutics, Inc.

Room: Grays/Longs

Speaker 1 of 6
* Peter S. Linsley, AVI BioPharma, USA

Sunday, April 18 | 8:00AM - 11:00AM
Development of Gene-Specific Double-Stranded RNA Drugs. Ses
sion Sponsored by Sirna Therapeutics, Inc.

Room: Grays/Longs

Speaker 2 of 6
Sailen Barik, University of South Alabama, USA
RNA Interference Analysis of Host-Pathogen Signaling

Sunday, April 18 | 8:00AM - 11:00AM
Development of Gene-Specific Double-Stranded RNA Drugs. Ses
sion Sponsored by Sirna Therapeutics, Inc.

Room: Grays/Longs

Speaker 3 of 6
Victor R. Ambros, University of Massachusetts, USA
Classes of Small RNAs Associated with Widespread Gene Silencing in C. elegans

Sunday, April 18 | 8:00AM - 11:00AM
Development of Gene-Specific Double-Stranded RNA Drugs. Ses
sion Sponsored by Sirna Therapeutics, Inc.

Room: Grays/Longs

Speaker 4 of 6
Markus Stoffel, ETH Zürich, Switzerland
Pancreatic Islet-Specific miRNAs and Insulin Secretion

Sunday, April 18 | 8:00AM - 11:00AM
Development of Gene-Specific Double-Stranded RNA Drugs. Ses
sion Sponsored by Sirna Therapeutics, Inc.

Room: Grays/Longs

Speaker 5 of 6
Muthiah Manoharan, Alnylam Pharmaceuticals, Inc., USA
Short Talk: Chemically Modified Short Interfering RNAs: in vivo Systemic Delivery, Pharmacokinetics and Pharmacodynamics in Mouse

Sunday, April 18 | 8:00AM - 11:00AM
Development of Gene-Specific Double-Stranded RNA Drugs. Ses
sion Sponsored by Sirna Therapeutics, Inc.

Room: Grays/Longs

Speaker 6 of 6
David V. Morrissey, Triplet Therapeutics, Inc., USA
Short Talk: Progress in the Development of Therapeutic siRNAs Against Viral Hepatitis

Sunday, April 18 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Sunday, April 18 | 4:30PM - 5:00PM
Coffee & Snacks Available
Room: Longs Peak Foyer


Sunday, April 18 | 5:00PM - 7:00PM
Characterization of Small-RNA-Regulated Networks and Escape
from Regulation

Room: Grays/Longs

Speaker 1 of 6
* Craig C. Mello, University of Massachusetts Medical School, USA

Sunday, April 18 | 5:00PM - 7:00PM
Characterization of Small-RNA-Regulated Networks and Escape
from Regulation

Room: Grays/Longs

Speaker 2 of 6
Phillip A. Sharp, Massachusetts Institute of Technology, USA
Specificity of microRNAs and ES Cell-Specific Expression

Sunday, April 18 | 5:00PM - 7:00PM
Characterization of Small-RNA-Regulated Networks and Escape
from Regulation

Room: Grays/Longs

Speaker 3 of 6
Debora Marks, Columbia University, USA
Computational Biology of miRNA Regulation

Sunday, April 18 | 5:00PM - 7:00PM
Characterization of Small-RNA-Regulated Networks and Escape
from Regulation

Room: Grays/Longs

Speaker 4 of 6
Julius Brennecke, IMBA - Institut für Molekulare Biotechnologie GmbH, Austria
Short Talk: Identification of miRNA Targets in Drosophila

Sunday, April 18 | 5:00PM - 7:00PM
Characterization of Small-RNA-Regulated Networks and Escape
from Regulation

Room: Grays/Longs

Speaker 5 of 6
Erik Sontheimer, University of Massachusetts Medical School, USA
Short Talk: A Dicer-2-Dependent Assembly Pathway Generates an 80S
Silencing Complex in Drosophila


Sunday, April 18 | 5:00PM - 7:00PM
Characterization of Small-RNA-Regulated Networks and Escape
from Regulation

Room: Grays/Longs

Speaker 6 of 6
Ulrike Gaul, Rockefeller University, USA
Short Talk: miRNA Function in Drosophila Development

Sunday, April 18 | 7:00PM - 8:00PM
Social Hour
Room: Quandary


Sunday, April 18 | 8:00PM - 11:00PM
Entertainment
Room: Quandary


Sunday, April 18 | 8:00PM - 11:00PM
Cash Bar
Room: Quandary


Monday, April 19 | 10:21AM - 10:21AM
Departure


*Session Chair
†Invited, not yet responded.