Web Desc
Plant Cell Signaling: In vivo and omics Approaches
Organizer(s): Sarah M. Assmann and Alan M. Jones
Date: February 01 - 06, 2005
Location: Hilton Santa Fe Historic Plaza Hotel, Santa Fe, NM, USA
Supported by The Director's Fund
Summary of Meeting:
Much of the most exciting current research on cell signaling involves in vivo and large scale ('omic') techniques. In vivo techniques, especially those utilizing advanced GFP-based imaging methods, are being brought to bear on studies of signal transduction in 'real time' and 'in planta,' thus allowing never-before-seen glimpses into the functioning of living plant cells. At the same time, 'omic' techniques are providing us with overviews of the entire function of a cell or tissue at the levels of the genome, the transcriptome, the proteome, and the metabolome. This meeting will bring together leaders in cell signaling research, spanning the gamut from cell biologists who observe single cells, to genomicists uncovering whole-genome views of transcription, to mathematicians who seek to build testable models of cellular processes. Key signaling elements, including G proteins, hormonal signaling, and dynamic organization of the nucleus and cytoskeleton are some of the biological processes that are highlighted. Presentation of cutting-edge data and techniques, including a workshop on FRET methodology, and the Keystone Symposium format itself will promote discussion and collaboration among participants.
Discounted Abstract Deadline: September 30 2004
Discounted Registration Deadline: December 1 2004
We gratefully acknowledge additional support for this conference from:
Pioneer Hi-Bred a DuPont business
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Tuesday, February 01 | 3:00PM - 7:30PM
Registration
Room: Promenade


Tuesday, February 01 | 6:30PM - 7:30PM
Welcome Refreshments
Room: Promenade


Tuesday, February 01 | 7:30PM - 8:30PM
Keynote Address
Room: Mesa A-B

Speaker 1 of 1
Michael P. Snyder, Stanford University School of Medicine, USA
Analyze This and That: Genomes and Proteomes

Wednesday, February 02 | 7:00AM - 8:00AM
Breakfast
Room: Chamisa


Wednesday, February 02 | 8:00AM - 11:00AM
Hormones and Signal Transduction
Room: Mesa A-B

Speaker 1 of 7
Tatsuo Kakimoto, Osaka University, Japan
Cytokinin Signaling

Wednesday, February 02 | 8:00AM - 11:00AM
Hormones and Signal Transduction
Room: Mesa A-B

Speaker 2 of 7
Randy D. Allen, Oklahoma State University, USA
Short Talk: Brassinosteroid Signaling in Cotton Fiber Development

Wednesday, February 02 | 8:00AM - 11:00AM
Hormones and Signal Transduction
Room: Mesa A-B

Speaker 3 of 7
Frank L.H. Menke, Sainsbury Laboratory, UK
Short Talk: Phosphoproteomics of JA-Dependent Induced Systemic Rresistance

Wednesday, February 02 | 8:00AM - 11:00AM
Hormones and Signal Transduction
Room: Mesa A-B

Speaker 4 of 7
Jutta Ludwig-Müller, Technische Universitaet Dresden, Germany
Short Talk: Transcriptome Analysis of the Clubroot Infection of Arabidopsis thaliana Revealed Novel Roles for Plant Hormones as Signals during Root Gall Development

Wednesday, February 02 | 8:00AM - 11:00AM
Hormones and Signal Transduction
Room: Mesa A-B

Speaker 5 of 7
Claus Schwechheimer, Technische Universitat Munchen, Germany
Short Talk: Control of Plant Development by Kinases Interacting with DELLA-Domain Proteins.

Wednesday, February 02 | 8:00AM - 11:00AM
Hormones and Signal Transduction
Room: Mesa A-B

Speaker 6 of 7
Hak Soo Seo, Seoul National University, South Korea
Interaction of Sumoylation and Ubiquitination in Plant Cell Signaling

Wednesday, February 02 | 8:00AM - 11:00AM
Hormones and Signal Transduction
Room: Mesa A-B

Speaker 7 of 7
* Mark A. Estelle, University of California, San Diego, USA
Protein Turnover in Auxin Signaling

Wednesday, February 02 | 9:20AM - 9:40AM
Coffee Break
Room: Promenade


Wednesday, February 02 | 11:00AM - 1:00PM
Poster Setup
Room: Ortiz


Wednesday, February 02 | 1:00PM - 10:00PM
Poster Viewing
Room: Ortiz


Wednesday, February 02 | 4:30PM - 4:45PM
Coffee & Snacks Available
Room: Promenade


Wednesday, February 02 | 4:45PM - 7:00PM
Visualizing Molecular Activities in vivo
Room: Mesa A-B

Speaker 1 of 4
Jennifer A. Lippincott-Schwartz, Howard Hughes Medical Institute - Janelia Farm, USA
Photoactivatable GFPs and Intracellular Protein Dynamics


Wednesday, February 02 | 4:45PM - 7:00PM
Visualizing Molecular Activities in vivo
Room: Mesa A-B

Speaker 2 of 4
Klaus M. Hahn, University of North Carolina at Chapel Hill, USA
Novel dyes enable sensitive imaging of endogenous protein conformational changes in living cells - Cdc42 and Erk2

Wednesday, February 02 | 4:45PM - 7:00PM
Visualizing Molecular Activities in vivo
Room: Mesa A-B

Speaker 3 of 4
* Dorus Gadella, University of Amsterdam, Netherlands
Visualizing Cytoskeletal Dynamics and Cellular Signaling with GFP-Based Multimode Fluorescence Microscopy

Wednesday, February 02 | 4:45PM - 7:00PM
Visualizing Molecular Activities in vivo
Room: Mesa A-B

Speaker 4 of 4
Robyn M. Perrin, University of Wisconsin-Madison, USA
Short Talk: WVD2, a Novel Microtubule Binding Protein Affecting Plant Anisotropic Cell Expansion, Morphology and Helical Handedness

Wednesday, February 02 | 7:00PM - 8:00PM
Social Hour
Room: Chamisa


Wednesday, February 02 | 7:30PM - 10:00PM
Poster Session 1
Room: Ortiz


Thursday, February 03 | 7:00AM - 8:00AM
Breakfast
Room: Chamisa


Thursday, February 03 | 8:00AM - 11:15AM
G Protein Regulation of Cellular Processes
Room: Mesa A-B

Speaker 1 of 5
* Sarah M. Assmann, Pennsylvania State University, USA
Heterotrimeric G Protein Signaling in Arabidopsis ABA Responses

Thursday, February 03 | 8:00AM - 11:15AM
G Protein Regulation of Cellular Processes
Room: Mesa A-B

Speaker 2 of 5
Alan M. Jones, University of North Carolina at Chapel Hill, USA
Sugar Sensing in Plants Coupled by a Heterotrimeric G Protein

Thursday, February 03 | 8:00AM - 11:15AM
G Protein Regulation of Cellular Processes
Room: Mesa A-B

Speaker 3 of 5
Tian Jin, NIAID, National Institutes of Health, USA
Visualization of G-Protein Localization and Activity during Dictyostelium Chemotaxis

Thursday, February 03 | 8:00AM - 11:15AM
G Protein Regulation of Cellular Processes
Room: Mesa A-B

Speaker 4 of 5
Ralph Panstruga, Max-Planck-Institut für Züchtungsforschung, Germany
Serpentine Plant MLO Proteins as Entry Portals for Powdery Mildew Fungi

Thursday, February 03 | 8:00AM - 11:15AM
G Protein Regulation of Cellular Processes
Room: Mesa A-B

Speaker 5 of 5
Ko Shimamoto, Nara Institute of Science and Technology, Japan
G Proteins and Disease Resistance in Rice

Thursday, February 03 | 9:20AM - 9:40AM
Coffee Break
Room: Promenade


Thursday, February 03 | 11:15AM - 12:00PM
Poster Setup
Room: Ortiz


Thursday, February 03 | 11:15AM - 12:00PM
Lunch
Room: Chamisa


Thursday, February 03 | 12:00PM - 2:00PM
Workshop: FRET Techniques
Room: Mesa A-B

Speaker 1 of 7
* Alan M. Jones, University of North Carolina at Chapel Hill, USA

Thursday, February 03 | 12:00PM - 2:00PM
Workshop: FRET Techniques
Room: Mesa A-B

Speaker 2 of 7
J. Philip Taylor, University of North Carolina at Chapel Hill, USA
FRET Analysis in Plant Cells: Trials and Tribulations

Thursday, February 03 | 12:00PM - 2:00PM
Workshop: FRET Techniques
Room: Mesa A-B

Speaker 3 of 7
Tian Jin, NIAID, National Institutes of Health, USA
Spectrally Resolved FRET Time-Lapse Imaging Detects Dynamic Protein-Protein Interactions in Single Living Cells

Thursday, February 03 | 12:00PM - 2:00PM
Workshop: FRET Techniques
Room: Mesa A-B

Speaker 4 of 7
Simon Gilroy, University of Wisconsin, USA
Imaging Ionic Signaling in the Arabidopsis Root

Thursday, February 03 | 12:00PM - 2:00PM
Workshop: FRET Techniques
Room: Mesa A-B

Speaker 5 of 7
Raymond Zielinski, University of Illinois at Urbana-Champaign, USA
Fluorescent Reporters of Downstream Events in Ca2+ Signaling Pathways

Thursday, February 03 | 12:00PM - 2:00PM
Workshop: FRET Techniques
Room: Mesa A-B

Speaker 6 of 7
Albrecht G. Von Arnim, University of Tennessee, USA
In vivo Protein-Interaction Assays Based on Bioluminescence Resonance Energy Transfer (BRET)

Thursday, February 03 | 12:00PM - 2:00PM
Workshop: FRET Techniques
Room: Mesa A-B

Speaker 7 of 7
Karin Schumacher, University of Tuebingen, Germany
Bimolecular Fluorescence Complementation (BiFC) in Plant Cells

Thursday, February 03 | 2:00PM - 4:30PM
Demonstration of FRET Microscope Platforms and Software by O
lympus and Scanalytics
Room: Ortiz


Thursday, February 03 | 2:00PM - 4:30PM
Poster Session 2
Room: Ortiz


Thursday, February 03 | 4:30PM - 4:45PM
Coffee & Snacks Available
Room: Promenade


Thursday, February 03 | 4:45PM - 7:00PM
Intracellular Signaling I
Room: Mesa A-B

Speaker 1 of 4
Federica Brandizzi, Michigan State University, USA
In vivo Dynamics of the Plant Endomembrane System

Thursday, February 03 | 4:45PM - 7:00PM
Intracellular Signaling I
Room: Mesa A-B

Speaker 2 of 4
Ying Gu, USA
Short Talk: ROP GTPases and Cytoskeletal Dynamics in Pollen Tubes

Thursday, February 03 | 4:45PM - 7:00PM
Intracellular Signaling I
Room: Mesa A-B

Speaker 3 of 4
* Natasha V. Raikhel, University of California, Riverside, USA
The Power of Chemical Genomics to Discover Proteins that Mediate Endomembrane Trafficking in Plants

Thursday, February 03 | 4:45PM - 7:00PM
Intracellular Signaling I
Room: Mesa A-B

Speaker 4 of 4
Frederick M. Ausubel, Massachusetts General Hospital, USA
PAMP-Elicited Innate Immune Signaling Pathways in Arabidopsis

Thursday, February 03 | 7:00PM - 10:00PM
Poster Viewing or Free for Dinner
Room: Ortiz


Friday, February 04 | 7:00AM - 8:00AM
Breakfast
Room: Chamisa


Friday, February 04 | 8:00AM - 11:00AM
Intracellular Signaling II
Room: Mesa A-B

Speaker 1 of 5
Gareth H. Jones, University of Birmingham, UK
The Control of Meiotic Recombination: A Cytological Perspective

Friday, February 04 | 8:00AM - 11:00AM
Intracellular Signaling II
Room: Mesa A-B

Speaker 2 of 5
* Gregory P. Copenhaver, University of North Carolina at Chapel Hill, USA
A New View of Meiotic Recombination

Friday, February 04 | 8:00AM - 11:00AM
Intracellular Signaling II
Room: Mesa A-B

Speaker 3 of 5
Joanne Chory, The Salk Institute, USA
Plastid to Nucleus Signaling

Friday, February 04 | 8:00AM - 11:00AM
Intracellular Signaling II
Room: Mesa A-B

Speaker 4 of 5
Maureen R. Hanson, Cornell University, USA
Short Talk: Intracellular Communication Through Stromules, Mobile Tubular Plastid Projections

Friday, February 04 | 8:00AM - 11:00AM
Intracellular Signaling II
Room: Mesa A-B

Speaker 5 of 5
Masamitsu Wada, Kyushu University, Japan
Light-Induced Chloroplast Relocation Movement

Friday, February 04 | 9:20AM - 9:40AM
Coffee Break
Room: Promenade


Friday, February 04 | 11:00AM - 1:00PM
Poster Setup
Room: Ortiz


Friday, February 04 | 1:00PM - 10:00PM
Poster Viewing
Room: Ortiz


Friday, February 04 | 4:30PM - 4:45PM
Coffee & Snacks Available
Room: Promenade


Friday, February 04 | 4:45PM - 7:00PM
Single Cells and New Techniques
Room: Mesa A-B

Speaker 1 of 5
Wolf B. Frommer, Carnegie Institution for Science, USA
Metabolite Imaging with FRET-Based Nanosensors


Friday, February 04 | 4:45PM - 7:00PM
Single Cells and New Techniques
Room: Mesa A-B

Speaker 2 of 5
* Simon Gilroy, University of Wisconsin, USA
Ca2+ and pH Integrate Tropic Signaling in the Root

Friday, February 04 | 4:45PM - 7:00PM
Single Cells and New Techniques
Room: Mesa A-B

Speaker 3 of 5
Soren Friis, Royal Veterinary and Agricultural University, Denmark
Short Talk: Novel GFP Based pH-Sensors for Monitoring H+-ATPase Activity in Plant Root Hair Cells of Arabidopsis thaliana

Friday, February 04 | 4:45PM - 7:00PM
Single Cells and New Techniques
Room: Mesa A-B

Speaker 4 of 5
Helen E. Blackwell, University of Wisconsin-Madison, USA
Small Molecule Control of Plant/Microbe Interactions

Friday, February 04 | 4:45PM - 7:00PM
Single Cells and New Techniques
Room: Mesa A-B

Speaker 5 of 5
Patrick S. Schnable, Iowa State University, USA
Laser Capture Microdissection and Cell-Type-Specific Gene Expression

Friday, February 04 | 7:00PM - 8:00PM
Social Hour
Room: Chamisa


Friday, February 04 | 7:30PM - 10:00PM
Poster Session 3
Room: Ortiz


Saturday, February 05 | 7:00AM - 8:00AM
Breakfast
Room: Chamisa


Saturday, February 05 | 8:00AM - 11:00AM
Cellomics I
Room: Mesa A-B

Speaker 1 of 5
* Julian I. Schroeder, University of California, San Diego, USA
Guard Cell Signal Transduction from Genomics to Cellular Signaling Dynamics

Saturday, February 05 | 8:00AM - 11:00AM
Cellomics I
Room: Mesa A-B

Speaker 2 of 5
Bernhard O. Palsson, University of California, San Diego, USA
Bringing Genomes to Life: The Use of Genome-Scale in Silico Models

Saturday, February 05 | 8:00AM - 11:00AM
Cellomics I
Room: Mesa A-B

Speaker 3 of 5
Waltraud Schulze, University of Southern Denmark, Denmark
Short Talk: Proteomic Strategies to Analyze Modification-Dependent Protein-Protein Interactions

Saturday, February 05 | 8:00AM - 11:00AM
Cellomics I
Room: Mesa A-B

Speaker 4 of 5
Daphne Preuss, Chromatin Inc., USA
Proteomics: Pollen Coat Proteins and Ecotypic Variation

Saturday, February 05 | 8:00AM - 11:00AM
Cellomics I
Room: Mesa A-B

Speaker 5 of 5
Marcus Heisler, EMBL Heidelberg, Germany
The Computable Plant

Saturday, February 05 | 9:20AM - 9:40AM
Coffee Break
Room: Promenade


Saturday, February 05 | 4:30PM - 4:45PM
Coffee & Snacks Available
Room: Promenade


Saturday, February 05 | 4:45PM - 7:00PM
Cellomics II
Room: Mesa A-B

Speaker 1 of 4
* Joseph R. Ecker, The Salk Institute for Biological Studies, USA
Genome-Wide Discovery of Transcription Units and Functional Elements in Arabidopsis

Saturday, February 05 | 4:45PM - 7:00PM
Cellomics II
Room: Mesa A-B

Speaker 2 of 4
Mark Stitt, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Germany
Transcriptomics, 'Nzynomics and Metabolomics of Carbon and Nitrogen Assimilation

Saturday, February 05 | 4:45PM - 7:00PM
Cellomics II
Room: Mesa A-B

Speaker 3 of 4
Pierre Broun, University of York, UK
Short Talk: A Genomic Investigation of Metabolic Regulation in Tomato Trichome Secretory Cells

Saturday, February 05 | 4:45PM - 7:00PM
Cellomics II
Room: Mesa A-B

Speaker 4 of 4
Scott C. Peck, University of Missouri, USA
Phosphoproteomics of Plant-Microbe Interactions: New Components, New Levels of Complexity

Saturday, February 05 | 6:45PM - 7:00PM
The Future
Room: Mesa A-B

Speaker 1 of 2
Joseph R. Ecker, The Salk Institute for Biological Studies, USA

Saturday, February 05 | 6:45PM - 7:00PM
The Future
Room: Mesa A-B

Speaker 2 of 2
Daphne Preuss, Chromatin Inc., USA

Saturday, February 05 | 7:00PM - 8:00PM
Social Hour
Room: Mesa C


Saturday, February 05 | 8:00PM - 11:00PM
Entertainment
Room: Mesa C


Saturday, February 05 | 8:00PM - 11:00PM
Cash Bar
Room: Mesa C


Sunday, February 06 | 10:26AM - 10:26AM
Departure


*Session Chair.