Web Desc
RNAi, MicroRNA, and Non-Coding RNA
Organizer(s): Judy Lieberman and Phillip A. Sharp
Date: March 25 - 30, 2008
Location: Whistler Conference Centre, Whistler, BC, Canada
Sponsored by Alnylam Pharmaceuticals, Inc., Novartis Institutes for BioMedical Research, and Santaris Pharma A/S
Summary of Meeting:
RNA interference (RNAi) describes a variety of evolutionarily conserved mechanisms for regulating gene transcription and post-transcriptional events that are important for controlling development and cell proliferation, survival, differentiation and effector functions. RNAi also protects the genome from transposons and viruses. This meeting will explore recent developments in understanding the molecular mechanisms and cell biology of RNA-based post-transcriptional silencing and for maintaining regions of silenced chromatin. It will consider methods to uncover regulatory networks of endogenous microRNAs and their target genes and illustrative examples in a variety of organisms. Recent advances in harnessing RNA interference for genetic screening and therapeutic gene silencing will also be explored.
We gratefully acknowledge additional support for this conference from:
Alnylam Pharmaceuticals, Inc.Integrated DNA TechnologiesIsis Pharmaceuticals, Inc.Luminex CorporationMerck & Co., Inc.Novartis Institutes for BioMedical Research
QIAGEN, Inc.
Regulus Therapeutics Inc.Santaris Pharma A/S
We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements:

Isis Pharmaceuticals, Inc.

Mirus Bio LLC
We gratefully acknowledge the generous grant for this conference provided by:

National Cancer Institute (NCI)
Grant No. 1R13CA132336-01
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Tuesday, March 25 | 3:00PM - 7:30PM
Registration
Room: Grand Foyer


Tuesday, March 25 | 6:30PM - 7:30PM
Refreshments
Room: Grand Foyer


Tuesday, March 25 | 7:30PM - 9:30PM
Keynote Session
Room: Sea to Sky Ballroom BC

Speaker 1 of 4
* Judy Lieberman, Harvard University, USA
and

Tuesday, March 25 | 7:30PM - 9:30PM
Keynote Session
Room: Sea to Sky Ballroom BC

Speaker 2 of 4
* Phillip A. Sharp, Massachusetts Institute of Technology, USA
Introductions

Tuesday, March 25 | 7:30PM - 9:30PM
Keynote Session
Room: Sea to Sky Ballroom BC

Speaker 3 of 4
Craig C. Mello, University of Massachusetts Medical School, USA
Small RNA Pathways and Germline in C. elegans

Tuesday, March 25 | 7:30PM - 9:30PM
Keynote Session
Room: Sea to Sky Ballroom BC

Speaker 4 of 4
Gregory J. Hannon, University of Cambridge, UK
Conserved Roles of Small RNAs in Genome Defense

Wednesday, March 26 | 7:00AM - 8:00AM
Breakfast
Room: Sea to Sky Ballroom A, Lower Level


Wednesday, March 26 | 8:00AM - 11:00AM
MicroRNAs and Their Targets (Vertebrate). Session Supported
by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 1 of 5
David P. Bartel, Massachusetts Institute of Technology and Whitehead Institute, USA
MicroRNAs and their Targets

Wednesday, March 26 | 8:00AM - 11:00AM
MicroRNAs and Their Targets (Vertebrate). Session Supported
by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 2 of 5
Carlo M. Croce, Ohio State University, USA
MicroRNA Genes and Cancer

Wednesday, March 26 | 8:00AM - 11:00AM
MicroRNAs and Their Targets (Vertebrate). Session Supported
by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 3 of 5
Eric N. Olson, University of Texas Southwestern Medical Center, USA
microRNAs Control of Muscle Development and Disease

Wednesday, March 26 | 8:00AM - 11:00AM
MicroRNAs and Their Targets (Vertebrate). Session Supported
by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 4 of 5
* V. Narry Kim, Institute for Basic Science, South Korea
lin 28-Mediated Terminal Uridylation of pre-let-7

Wednesday, March 26 | 8:00AM - 11:00AM
MicroRNAs and Their Targets (Vertebrate). Session Supported
by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 5 of 5
Joel G. Belasco, New York University School of Medicine, USA
Short Talk: Importance of Translational Repression and non-Nucleolytic Ago Isoforms for on-Target RNA Interference

Wednesday, March 26 | 9:20AM - 9:40AM
Coffee Break
Room: Grand Foyer


Wednesday, March 26 | 11:00AM - 1:00PM
Poster Setup
Room: Sea to Sky Ballroom A, Foyer


Wednesday, March 26 | 11:00AM - 11:00AM
On Own for Lunch


Wednesday, March 26 | 1:00PM - 10:00PM
Poster Viewing
Room: Sea to Sky Ballroom A, Foyer


Wednesday, March 26 | 4:30PM - 5:00PM
Coffee Available
Room: Grand Foyer


Wednesday, March 26 | 5:00PM - 7:15PM
MicroRNAs and Their Targets (Plants and Invertebrates). Ses
sion Supported by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 1 of 7
* Victor R. Ambros, University of Massachusetts, USA

Wednesday, March 26 | 5:00PM - 7:15PM
MicroRNAs and Their Targets (Plants and Invertebrates). Ses
sion Supported by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 2 of 7
Stephen M. Cohen, Institute of Molecular and Cell Biology, Singapore
MicroRNA Functions

Wednesday, March 26 | 5:00PM - 7:15PM
MicroRNAs and Their Targets (Plants and Invertebrates). Ses
sion Supported by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 3 of 7
Oliver Hobert, Columbia University, USA
The Regulatory Architecture of a miRNA-Controlled 3'UTR

Wednesday, March 26 | 5:00PM - 7:15PM
MicroRNAs and Their Targets (Plants and Invertebrates). Ses
sion Supported by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 4 of 7
Molly C. Hammell, Cold Spring Harbor Laboratory, USA
Short Talk: mirWIP: microRNA Target Prediction Based on miRNP Enriched Transcripts

Wednesday, March 26 | 5:00PM - 7:15PM
MicroRNAs and Their Targets (Plants and Invertebrates). Ses
sion Supported by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 5 of 7
Marcelo A. German, Dow AgroSciences, USA
Short Talk: Global Identification of miRNA-Target RNA Pairs by Parallel Analysis of RNA Ends (PARE)

Wednesday, March 26 | 5:00PM - 7:15PM
MicroRNAs and Their Targets (Plants and Invertebrates). Ses
sion Supported by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 6 of 7
Eric C. Lai, Sloan Kettering Institute, USA
Short Talk: Biogenesis and Function of Hairpin RNAs

Wednesday, March 26 | 5:00PM - 7:15PM
MicroRNAs and Their Targets (Plants and Invertebrates). Ses
sion Supported by Regulus Therapeutics

Room: Sea to Sky Ballroom BC

Speaker 7 of 7
Alla Grishok, Columbia University Medical Center, USA
Short Talk: Targets of Endogenous RNAi Processes in C. elegans

Wednesday, March 26 | 7:15PM - 8:15PM
Social Hour with Lite Bites
Room: Sea to Sky Ballroom A, Foyer


Wednesday, March 26 | 7:30PM - 10:00PM
Poster Session 1
Room: Sea to Sky Ballroom A, Foyer


Thursday, March 27 | 7:00AM - 8:00AM
Breakfast
Room: Sea to Sky Ballroom A, Lower Level


Thursday, March 27 | 8:00AM - 11:15AM
RNA Interference: Regulation of Transcription
Room: Sea to Sky Ballroom BC

Speaker 1 of 5
Danesh Moazed, Harvard Medical School, USA
Role of Non-Coding RNAs in Heterochromatin Assembly

Thursday, March 27 | 8:00AM - 11:15AM
RNA Interference: Regulation of Transcription
Room: Sea to Sky Ballroom BC

Speaker 2 of 5
* Marjori Ann Matzke, Institute of Plant and Microbial Biology, Academy Sinica, Taiwan
RNA-Directed DNA Methylation in Arabidopsis

Thursday, March 27 | 8:00AM - 11:15AM
RNA Interference: Regulation of Transcription
Room: Sea to Sky Ballroom BC

Speaker 3 of 5
Alexei A. Aravin, California Institute of Technology, USA
Small RNA Pathways in Mouse Germline

Thursday, March 27 | 8:00AM - 11:15AM
RNA Interference: Regulation of Transcription
Room: Sea to Sky Ballroom BC

Speaker 4 of 5
René F. Ketting, Institute for Molecular Biology, Germany
TRF-Like Terminal Transferases affect RNAi and Chromosome Segregation in C. elegans

Thursday, March 27 | 8:00AM - 11:15AM
RNA Interference: Regulation of Transcription
Room: Sea to Sky Ballroom BC

Speaker 5 of 5
Michel A.J. Georges, University of Leige GIGA Institute B34, Belgium
Small RNAs and Imprinting

Thursday, March 27 | 9:20AM - 9:40AM
Coffee Break
Room: Grand Foyer


Thursday, March 27 | 11:15AM - 1:00PM
Poster Setup
Room: Sea to Sky Ballroom A, Foyer


Thursday, March 27 | 11:15AM - 11:15AM
On Own for Lunch


Thursday, March 27 | 1:00PM - 10:00PM
Poster Viewing
Room: Sea to Sky Ballroom A, Foyer


Thursday, March 27 | 4:30PM - 5:00PM
Coffee Available
Room: Grand Foyer


Thursday, March 27 | 5:00PM - 7:00PM
Non-coding RNAs
Room: Sea to Sky Ballroom BC

Speaker 1 of 4
* Thomas R. Gingeras, Cold Spring Harbor Laboratory, USA
Investigations of the Functional Roles of the Pervasive Transcription Observed for Genomes

Thursday, March 27 | 5:00PM - 7:00PM
Non-coding RNAs
Room: Sea to Sky Ballroom BC

Speaker 2 of 4
Gene W. Yeo, University of California, San Diego, USA
Short Talk: Analysis of Small RNAs in Stem Cell Development

Thursday, March 27 | 5:00PM - 7:00PM
Non-coding RNAs
Room: Sea to Sky Ballroom BC

Speaker 3 of 4
Dinshaw J. Patel, Memorial Sloan Kettering Cancer Center, USA
Structural Biology of Riboswitch-Mediated Gene Regulation and Argonaute-Mediated Gene Silencing

Thursday, March 27 | 5:00PM - 7:00PM
Non-coding RNAs
Room: Sea to Sky Ballroom BC

Speaker 4 of 4
Nikolaus Rajewsky, Max Delbruck Center for Molecular Medicine, Germany
Identifying MicroRNAs and Their Targets


Thursday, March 27 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Sea to Sky Ballroom A, Foyer


Thursday, March 27 | 7:30PM - 10:00PM
Poster Session 2
Room: Sea to Sky Ballroom A, Foyer


Friday, March 28 | 7:00AM - 8:00AM
Breakfast
Room: Sea to Sky Ballroom A, Lower Level


Friday, March 28 | 7:00AM - 8:00AM
Poster Setup
Room: Sea to Sky Ballroom A, Foyer


Friday, March 28 | 8:00AM - 11:00AM
RNA Interference: Newly Emerging Biology
Room: Sea to Sky Ballroom BC

Speaker 1 of 5
* Phillip D. Zamore, University of Massachusetts Medical School, USA
Germline and Somatic Small RNAs

Friday, March 28 | 8:00AM - 11:00AM
RNA Interference: Newly Emerging Biology
Room: Sea to Sky Ballroom BC

Speaker 2 of 5
Thomas Tuschl, HHMI/Rockefeller University, USA
miRNA Profiling and in Situ Hybridization to Examine Their Cell-Type Specific Expression Patterns

Friday, March 28 | 8:00AM - 11:00AM
RNA Interference: Newly Emerging Biology
Room: Sea to Sky Ballroom BC

Speaker 3 of 5
Haifan Lin, Yale University, USA
Epigenetic Function of Piwi and piRNAs

Friday, March 28 | 8:00AM - 11:00AM
RNA Interference: Newly Emerging Biology
Room: Sea to Sky Ballroom BC

Speaker 4 of 5
Phillip A. Sharp, Massachusetts Institute of Technology, USA
miRNA Silencing by Translational Inhibition and mRNA Degradation

Friday, March 28 | 8:00AM - 11:00AM
RNA Interference: Newly Emerging Biology
Room: Sea to Sky Ballroom BC

Speaker 5 of 5
Elisa Izaurralde, Max Planck Institute for Developmental Biology, Germany
Short Talk: Mechanism of miRNA-Mediated Gene Silencing

Friday, March 28 | 9:20AM - 9:40AM
Coffee Break
Room: Grand Foyer


Friday, March 28 | 11:00AM - 12:00PM
Lunch
Room: Sea to Sky Ballroom A, Foyer


Friday, March 28 | 12:00PM - 2:30PM
Poster Session 3
Room: Sea to Sky Ballroom A, Foyer


Friday, March 28 | 2:30PM - 4:30PM
Workshop 1: New Approaches to shRNA-Based Screening and Ther
apy
Room: Sea to Sky Ballroom BC

Speaker 1 of 5
David E. Root, Broad Institute, USA
Pooled shRNA Screens for Functional Fingerprinting

Friday, March 28 | 2:30PM - 4:30PM
Workshop 1: New Approaches to shRNA-Based Screening and Ther
apy
Room: Sea to Sky Ballroom BC

Speaker 2 of 5
Edward Harlow, Constellation Pharmaceuticals, USA
shRNA Screens to Identify Targets for Cancer Drug Development

Friday, March 28 | 2:30PM - 4:30PM
Workshop 1: New Approaches to shRNA-Based Screening and Ther
apy
Room: Sea to Sky Ballroom BC

Speaker 3 of 5
Michael T. Hemann, Massachusetts Institute of Technology, USA
In Vivo Screens to Identify Mechanisms of Drug Resistance

Friday, March 28 | 2:30PM - 4:30PM
Workshop 1: New Approaches to shRNA-Based Screening and Ther
apy
Room: Sea to Sky Ballroom BC

Speaker 4 of 5
Mark A. Kay, Stanford University, USA
Functional Evidence for Restricting MicroRNA Targets to the 3' Untranslated Region of mRNAs


Friday, March 28 | 2:30PM - 4:30PM
Workshop 1: New Approaches to shRNA-Based Screening and Ther
apy
Room: Sea to Sky Ballroom BC

Speaker 5 of 5
* John J. Rossi, Beckman Research Institute of the City of Hope, USA
Applying RNAi for the treatment of HIV infection

Friday, March 28 | 4:30PM - 5:00PM
Coffee Available
Room: Grand Foyer


Friday, March 28 | 5:00PM - 7:00PM
RNA Interference: Mechanisms and Cell Biology
Room: Sea to Sky Ballroom BC

Speaker 1 of 4
Matthias W. Hentze, European Molecular Biology Laboratory, Germany
Mechanism of Translational Inhibition by miRNAs

Friday, March 28 | 5:00PM - 7:00PM
RNA Interference: Mechanisms and Cell Biology
Room: Sea to Sky Ballroom BC

Speaker 2 of 4
Carl D. Novina, Dana-Farber Cancer Institute, USA
Short Talk: MicroRNA-Targeted mRNAs Contain 40S, but not 60S Components

Friday, March 28 | 5:00PM - 7:00PM
RNA Interference: Mechanisms and Cell Biology
Room: Sea to Sky Ballroom BC

Speaker 3 of 4
* Witold Filipowicz, Friedrich Miescher Institute for Biomedical Research, Switzerland
Role of Dicer in miRNA Pathways and Beyond

Friday, March 28 | 5:00PM - 7:00PM
RNA Interference: Mechanisms and Cell Biology
Room: Sea to Sky Ballroom BC

Speaker 4 of 4
Jennifer A. Doudna, HHMI/University of California, Berkeley, USA
Mechanisms of Small RNA Processing

Friday, March 28 | 7:00PM - 7:00PM
On Own for Dinner


Saturday, March 29 | 7:00AM - 8:00AM
Breakfast
Room: Sea to Sky Ballroom A, Lower Level


Saturday, March 29 | 7:00AM - 8:00AM
Poster Setup
Room: Sea to Sky Ballroom A, Foyer


Saturday, March 29 | 8:00AM - 11:00AM
RNAi-Based Screening
Room: Sea to Sky Ballroom BC

Speaker 1 of 4
* Gary B. Ruvkun, Massachusetts General Hospital, Harvard Medical School, USA
The Protein Coding Cofactors of miRNAs and siRNAs

Saturday, March 29 | 8:00AM - 11:00AM
RNAi-Based Screening
Room: Sea to Sky Ballroom BC

Speaker 2 of 4
Anjana Rao, La Jolla Institute for Allergy and Immunology, USA
RNAi Screen for Mammalian Biology and a Bit About Eri-1

Saturday, March 29 | 8:00AM - 11:00AM
RNAi-Based Screening
Room: Sea to Sky Ballroom BC

Speaker 3 of 4
Reuven Agami, Netherlands Cancer Institute, Netherlands
Dead End 1 Blocks microRNA Access to Target mRNA

Saturday, March 29 | 8:00AM - 11:00AM
RNAi-Based Screening
Room: Sea to Sky Ballroom BC

Speaker 4 of 4
Scott M. Hammond, University of North Carolina at Chapel Hill, USA
Regulation of microRNA Biogenesis by Lin-28

Saturday, March 29 | 9:20AM - 9:40AM
Coffee Break
Room: Grand Foyer


Saturday, March 29 | 11:00AM - 12:00PM
Lunch
Room: Sea to Sky Ballroom A, Foyer


Saturday, March 29 | 12:00PM - 2:30PM
Poster Session 4
Room: Sea to Sky Ballroom A, Foyer


Saturday, March 29 | 2:30PM - 4:30PM
Workshop 2: Translating RNAi
Room: Sea to Sky Ballroom BC

Speaker 1 of 5
Lee Lim, Sirna/Merck, USA
Using Profiling to Study miRNAs

Saturday, March 29 | 2:30PM - 4:30PM
Workshop 2: Translating RNAi
Room: Sea to Sky Ballroom BC

Speaker 2 of 5
Muthiah Manoharan, Alnylam Pharmaceuticals, Inc., USA
Cellular Delivery of siRNAs and Antagomirs Using Chemical
Conjugation and Complexation Strategies


Saturday, March 29 | 2:30PM - 4:30PM
Workshop 2: Translating RNAi
Room: Sea to Sky Ballroom BC

Speaker 3 of 5
* C. Frank Bennett, Ionis Pharmaceuticals, Inc., USA
Therapeutic Approaches for Modulating Gene Expression Utilizing Single Stranded Oligonucleotides

Saturday, March 29 | 2:30PM - 4:30PM
Workshop 2: Translating RNAi
Room: Sea to Sky Ballroom BC

Speaker 4 of 5
David L. Lewis, Arrowhead Research Corporation, USA
Dynamic PolyConjugates: A Rationally Designed, Modular Platform for Systemic siRNA Delivery

Saturday, March 29 | 2:30PM - 4:30PM
Workshop 2: Translating RNAi
Room: Sea to Sky Ballroom BC

Speaker 5 of 5
Fumitaka Takeshita, National Cancer Center Research Institute, Japan
Systemic microRNA Therapy for Cancer Metastasis

Saturday, March 29 | 4:30PM - 5:00PM
Coffee Available
Room: Grand Foyer


Saturday, March 29 | 5:00PM - 7:00PM
RNAi-Based Therapy
Room: Sea to Sky Ballroom BC

Speaker 1 of 4
* Beverly L. Davidson, Children’s Hospital of Philadelphia and University of Pennsylvania, USA
RNAi and Neurodegenerative Diseases

Saturday, March 29 | 5:00PM - 7:00PM
RNAi-Based Therapy
Room: Sea to Sky Ballroom BC

Speaker 2 of 4
Judy Lieberman, Harvard University, USA
Silent Treatment for Cancer

Saturday, March 29 | 5:00PM - 7:00PM
RNAi-Based Therapy
Room: Sea to Sky Ballroom BC

Speaker 3 of 4
Markus Stoffel, ETH Zürich, Switzerland
Molecular Mechanisms of Cellular Uptake of Cholesterol-Conjugated RNAs

Saturday, March 29 | 5:00PM - 7:00PM
RNAi-Based Therapy
Room: Sea to Sky Ballroom BC

Speaker 4 of 4
Morten Lindow, Santaris Pharma, Denmark
Short Talk: LNA-Mediated microRNA Silencing in non-Human Primates

Saturday, March 29 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Sea to Sky Ballroom A, Foyer


Saturday, March 29 | 8:00PM - 11:00PM
Entertainment
Room: Sea to Sky Ballroom A, Foyer


Saturday, March 29 | 8:00PM - 11:00PM
Cash Bar
Room: Sea to Sky Ballroom A, Foyer


Sunday, March 30 | 10:25AM - 10:25AM
Departure


*Session Chair.