Web Desc
RNA Silencing: Mechanism, Biology and Application
Organizer(s): Phillip D. Zamore and Beverly L. Davidson
Date: January 14 - 19, 2010
Location: Keystone Resort, Keystone, CO, USA
Sponsored by Alnylam Pharmaceuticals, Inc., Isis Pharmaceuticals, Inc., Regulus Therapeutics, Inc. and Santaris Pharma A/S
Summary of Meeting:
Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small RNA classes have been identified, including microRNAs, various types of siRNAs and piRNAs. These classes differ in their biogenesis, modes of target regulation and in the biological pathways they regulate. However, there is also a growing realization that these distinct small RNA pathways are interconnected. These pathways functions in RNA-based immunity, developmental gene regulation, transcriptional silencing, and genomic stability. The aim of this meeting is to explore the similarities and differences between RNA silencing pathways, to probe their underlying molecular mechanisms, to learn—by illustrative examples—the logic behind RNA-guided regulation of gene expression, and to explore new methods for harnessing these mechanisms for gene discovery and therapeutics applications.
Scholarship Deadline: September 17 2009
Discounted Abstract Deadline: September 17 2009
Abstract Deadline: October 15 2009
Discounted Registration Deadline: November 16 2009
We gratefully acknowledge additional support for this conference from:
Alnylam Pharmaceuticals, Inc.Integrated DNA TechnologiesIsis Pharmaceuticals, Inc.Promega CorporationRegulus Therapeutics Inc.Santa Cruz Biotechnology, Inc.Santaris Pharma A/SThermo Fisher Scientific, Inc.


Mikihito Shibata
We gratefully acknowledge the generous grant for this conference provided by:

Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
Grant No. 1R13HD063228-01
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Thursday, January 14 | 3:00PM - 7:30PM
Registration
Room: Longs Peak Foyer


Thursday, January 14 | 6:30PM - 7:30PM
Refreshments
Room: Longs Peak Foyer


Thursday, January 14 | 7:30PM - 9:30PM
Keynote Session
Room: Longs/Quandary Peaks

Speaker 1 of 2
James C. Carrington, Donald Danforth Plant Science Center, USA
Small RNA-Directed Silencing Pathways in Plants

Thursday, January 14 | 7:30PM - 9:30PM
Keynote Session
Room: Longs/Quandary Peaks

Speaker 2 of 2
V. Narry Kim, Institute for Basic Science, South Korea
The Making of a MicroRNA

Friday, January 15 | 7:00AM - 8:00AM
Breakfast
Room: Grays/Torreys Peaks


Friday, January 15 | 8:00AM - 11:00AM
Structure and Regulation of Small RNAs
Room: Longs/Quandary Peaks

Speaker 1 of 5
Jennifer A. Doudna, HHMI/University of California, Berkeley, USA
Dicer and Beyond: Mechanisms of Regulatory RNA Processing and Targeting

Friday, January 15 | 8:00AM - 11:00AM
Structure and Regulation of Small RNAs
Room: Longs/Quandary Peaks

Speaker 2 of 5
Rachel J. Senturia, University of California, Los Angeles, USA
Short Talk: Crystallographic and Cellular Studies of the DGCR8 Dimerization Domain: A Platform for Interaction with Heme

Friday, January 15 | 8:00AM - 11:00AM
Structure and Regulation of Small RNAs
Room: Longs/Quandary Peaks

Speaker 3 of 5
Dinshaw J. Patel, Memorial Sloan Kettering Cancer Center, USA
A Structural Perspective of Recognition and Cleavage Events in RNA Silencing

Friday, January 15 | 8:00AM - 11:00AM
Structure and Regulation of Small RNAs
Room: Longs/Quandary Peaks

Speaker 4 of 5
Nina Schürmann, University of Heidelberg, Germany
Short Talk: Directed Molecular Evolution of Chimeric Argonaute Proteins as Potent New Approach to Elucidate Structure-Function Relationships of Human RISC

Friday, January 15 | 8:00AM - 11:00AM
Structure and Regulation of Small RNAs
Room: Longs/Quandary Peaks

Speaker 5 of 5
Martijn Kedde, Netherlands Cancer Institute, Netherlands
Short Talk: A Pumilio-Induced RNA Structure Switch in p27-3’UTR Controls miR-221&222 Accessibility

Friday, January 15 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Friday, January 15 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Friday, January 15 | 11:00AM - 1:00PM
Poster Setup
Room: Shavano/Torreys Peaks


Friday, January 15 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys Peaks


Friday, January 15 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Friday, January 15 | 5:00PM - 7:15PM
Regulation of Small RNA Pathways
Room: Longs/Quandary Peaks

Speaker 1 of 5
Brenda L. Bass, University of Utah, USA
A Role for Dicer's Helicase Domain

Friday, January 15 | 5:00PM - 7:15PM
Regulation of Small RNA Pathways
Room: Longs/Quandary Peaks

Speaker 2 of 5
Brandi N. Davis, Tufts Medical Center, USA
Short Talk: Smad Proteins Bind a Conserved RNA Sequence to Promote microRNA Maturation by Drosha

Friday, January 15 | 5:00PM - 7:15PM
Regulation of Small RNA Pathways
Room: Longs/Quandary Peaks

Speaker 3 of 5
Olivier Voinnet, Swiss Federal Institute of Technology, Zürich, Switzerland
Function and Regulation of miRNA Pathways in Plants

Friday, January 15 | 5:00PM - 7:15PM
Regulation of Small RNA Pathways
Room: Longs/Quandary Peaks

Speaker 4 of 5
Gary B. Ruvkun, Massachusetts General Hospital, Harvard Medical School, USA
Genetic Analysis Reveals the Complexity of the C. elegans RNA Silencing Pathways

Friday, January 15 | 5:00PM - 7:15PM
Regulation of Small RNA Pathways
Room: Longs/Quandary Peaks

Speaker 5 of 5
Guillaume Jannot, Laval University Cancer Research Centre, Canada
Short Talk: Conserved Function of a Ribosomal Protein RACK1 in the microRNA Pathway

Friday, January 15 | 7:15PM - 8:15PM
Social Hour with Lite Bites
Room: Shavano/Torreys Peaks


Friday, January 15 | 7:30PM - 10:00PM
Poster Session 1
Room: Shavano/Torreys Peaks


Saturday, January 16 | 7:00AM - 8:00AM
Breakfast
Room: Grays/Torreys Peaks


Saturday, January 16 | 8:00AM - 11:15AM
miRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 1 of 6
Xuemei Chen, University of California, Riverside, USA
Three Polymerases in siRNA-Directed Transcriptional Gene Silencing

Saturday, January 16 | 8:00AM - 11:15AM
miRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 2 of 6
Elisa Izaurralde, Max Planck Institute for Developmental Biology, Germany
The silencing domain of GW182 interact with PABPC1 to prompt silencing of miRNA targets

Saturday, January 16 | 8:00AM - 11:15AM
miRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 3 of 6
Demian E. Cazalla, University of Utah, USA
Short Talk: Herpesvirus saimiri U RNAs Affect the Expression of Cellular microRNAs in Latently Infected T Cells

Saturday, January 16 | 8:00AM - 11:15AM
miRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 4 of 6
David P. Bartel, Massachusetts Institute of Technology and Whitehead Institute, USA
MicroRNAs: Genomics, Targets, and the Molecular Consequences of Targeting

Saturday, January 16 | 8:00AM - 11:15AM
miRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 5 of 6
Victor R. Ambros, University of Massachusetts, USA
Modulators of microRNA Activity

Saturday, January 16 | 8:00AM - 11:15AM
miRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 6 of 6
Catherine A. Reinke, Northwestern University, USA
Short Talk: Elucidation of the miRNA Pathway through the Identification and Characterization of Genes Identified in a Forward Genetics Study of miRNA Function in Drosophila melanogaster

Saturday, January 16 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Saturday, January 16 | 11:15AM - 11:15AM
On Own for Lunch and Recreation


Saturday, January 16 | 11:15AM - 1:00PM
Poster Setup
Room: Shavano/Torreys Peaks


Saturday, January 16 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys Peaks


Saturday, January 16 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Saturday, January 16 | 5:00PM - 7:30PM
Small RNA Therapeutics
Room: Longs/Quandary Peaks

Speaker 1 of 5
Priti Kumar, Yale School of Medicine, USA
Targeting siRNAs to T-Cells for Anti-Viral Therapy

Saturday, January 16 | 5:00PM - 7:30PM
Small RNA Therapeutics
Room: Longs/Quandary Peaks

Speaker 2 of 5
Daniel G. Anderson, Massachusetts Institute of Technology, USA
Combinatorial Development of Synthetic siRNA Delivery Systems

Saturday, January 16 | 5:00PM - 7:30PM
Small RNA Therapeutics
Room: Longs/Quandary Peaks

Speaker 3 of 5
Mark A. Kay, Stanford University, USA
Mechanism of MicroRNA Mediated Transgene Silencing

Saturday, January 16 | 5:00PM - 7:30PM
Small RNA Therapeutics
Room: Longs/Quandary Peaks

Speaker 4 of 5
Lukas Jeker, University of Basel, Switzerland
Short Talk: miR-10a in Regulatory T Cells

Saturday, January 16 | 5:00PM - 7:30PM
Small RNA Therapeutics
Room: Longs/Quandary Peaks

Speaker 5 of 5
Sakari Kauppinen, Aalborg University, Denmark
Targeting of microRNAs for Therapeutics

Saturday, January 16 | 7:30PM - 8:30PM
Social Hour with Lite Bites
Room: Shavano/Torreys Peaks


Saturday, January 16 | 7:30PM - 10:00PM
Poster Session 2
Room: Shavano/Torreys Peaks


Sunday, January 17 | 7:00AM - 8:00AM
Breakfast
Room: Grays/Torreys Peaks


Sunday, January 17 | 8:00AM - 11:15AM
Small RNAs and RNAi I
Room: Longs/Quandary Peaks

Speaker 1 of 6
Attila Molnar, University of Cambridge, UK
Small RNAs are Mobile and Trigger DNA Methylation in Distant Tissue

Sunday, January 17 | 8:00AM - 11:15AM
Small RNAs and RNAi I
Room: Longs/Quandary Peaks

Speaker 2 of 6
Sarah C.R. Elgin, Washington University, USA
Targeting Heterochromatin Formation in Drosophila

Sunday, January 17 | 8:00AM - 11:15AM
Small RNAs and RNAi I
Room: Longs/Quandary Peaks

Speaker 3 of 6
Hanbang Zhang, Stanford University, USA
Short Talk: Antisense Small RNAs with 5’-Polyphosphate Termini Regulate Gene Expression in the Deep Branching Eukaryote Entamoeba Histolytica

Sunday, January 17 | 8:00AM - 11:15AM
Small RNAs and RNAi I
Room: Longs/Quandary Peaks

Speaker 4 of 6
Phillip A. Sharp, Massachusetts Institute of Technology, USA
MicroRNAs in mammalian cells---robustness

Sunday, January 17 | 8:00AM - 11:15AM
Small RNAs and RNAi I
Room: Longs/Quandary Peaks

Speaker 5 of 6
Elaine V. Fuchs, Rockefeller University, USA
Regulation in skin stem cell: Fine tuning by miRNA profile

Sunday, January 17 | 8:00AM - 11:15AM
Small RNAs and RNAi I
Room: Longs/Quandary Peaks

Speaker 6 of 6
Shouhong Guang, University of Science and Technology of China, China
Short Talk: Small RNAs Mediate Transcription Termination

Sunday, January 17 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Sunday, January 17 | 11:15AM - 11:15AM
On Own for Lunch and Recreation


Sunday, January 17 | 11:15AM - 1:00PM
Poster Setup
Room: Grays/Torreys Peaks


Sunday, January 17 | 1:00PM - 10:00PM
Poster Viewing
Room: Grays/Torreys Peaks


Sunday, January 17 | 2:30PM - 4:30PM
Workshop: piRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 1 of 4
Craig C. Mello, University of Massachusetts Medical School, USA
piRNAs in Worms: The Same, but Different

Sunday, January 17 | 2:30PM - 4:30PM
Workshop: piRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 2 of 4
Mikiko C. Siomi, University of Tokyo, Japan
piRNAs in the Fly Germ Line

Sunday, January 17 | 2:30PM - 4:30PM
Workshop: piRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 3 of 4
Gregory J. Hannon, University of Cambridge, UK
Why is Ago catalysis conserved in vertebrate?

Sunday, January 17 | 2:30PM - 4:30PM
Workshop: piRNA Biogenesis and Function
Room: Longs/Quandary Peaks

Speaker 4 of 4
Phillip D. Zamore, University of Massachusetts Medical School, USA
Target directed destruction of small silencing RNAs

Sunday, January 17 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Sunday, January 17 | 5:00PM - 7:00PM
Small RNAs and RNAi II
Room: Longs/Quandary Peaks

Speaker 1 of 4
René F. Ketting, Institute for Molecular Biology, Germany
Piwi biology in Zebrafish

Sunday, January 17 | 5:00PM - 7:00PM
Small RNAs and RNAi II
Room: Longs/Quandary Peaks

Speaker 2 of 4
Danesh Moazed, Harvard Medical School, USA
Small RNAs that Trigger Heterochromatin Assembly and RNAi

Sunday, January 17 | 5:00PM - 7:00PM
Small RNAs and RNAi II
Room: Longs/Quandary Peaks

Speaker 3 of 4
Shiv I. S. Grewal, NCI, National Institutes of Health, USA
RNAi and Heterochromatin Factors Suppress Antisense RNAs

Sunday, January 17 | 5:00PM - 7:00PM
Small RNAs and RNAi II
Room: Longs/Quandary Peaks

Speaker 4 of 4
Stephan Emmerth, Friedrich Miescher Institute, Switzerland
Short Talk: Nuclear Retention of Fission Yeast Dicer Ensures Accurate Gene Expression and Heterochromatin Assembly

Sunday, January 17 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Grays/Torreys Peaks


Sunday, January 17 | 7:30PM - 10:00PM
Poster Session 3
Room: Grays/Torreys Peaks


Monday, January 18 | 7:00AM - 8:00AM
Breakfast
Room: Grays/Torreys Peaks


Monday, January 18 | 8:00AM - 11:00AM
Small RNAs, Disease and Development
Room: Longs/Quandary Peaks

Speaker 1 of 6
Alexander Y. Rudensky, HHMI/Memorial Sloan Kettering Cancer Center, USA
Role of miRNAs in T-Cell Function

Monday, January 18 | 8:00AM - 11:00AM
Small RNAs, Disease and Development
Room: Longs/Quandary Peaks

Speaker 2 of 6
Beverly L. Davidson, Children’s Hospital of Philadelphia and University of Pennsylvania, USA
Small RNAs and Huntington’s disease

Monday, January 18 | 8:00AM - 11:00AM
Small RNAs, Disease and Development
Room: Longs/Quandary Peaks

Speaker 3 of 6
Mark P. Boldin, City of Hope, USA
Short Talk: Myeloproliferative Disease, Autoimmunity and Cancer in Mice with Targeted Deletion of miR-146a Gene

Monday, January 18 | 8:00AM - 11:00AM
Small RNAs, Disease and Development
Room: Longs/Quandary Peaks

Speaker 4 of 6
Deepak Srivastava, Gladstone Institutes, USA
microRNA Regulation of Cardiac Cell Fate, Morphogenesis and Function

Monday, January 18 | 8:00AM - 11:00AM
Small RNAs, Disease and Development
Room: Longs/Quandary Peaks

Speaker 5 of 6
Magnus Blø, University of Bergen, Norway
Short Talk: Flow Cytometric-Based Functional Selection of RNA Interference Triggers for epi-allelic Analysis

Monday, January 18 | 8:00AM - 11:00AM
Small RNAs, Disease and Development
Room: Longs/Quandary Peaks

Speaker 6 of 6
Tudor A. Fulga, Vertex Genetic Therapies, USA
Short Talk: Defining microRNA Function with Precise Spatiotemporal Resolution in Intact Organisms

Monday, January 18 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Monday, January 18 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Monday, January 18 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Monday, January 18 | 5:00PM - 7:00PM
Small RNAs in Stem Cells and Germ Cells
Room: Longs/Quandary Peaks

Speaker 1 of 4
Gene W. Yeo, University of California, San Diego, USA
Pinning down microRNA and Targeting in C. elegants and Humans

Monday, January 18 | 5:00PM - 7:00PM
Small RNAs in Stem Cells and Germ Cells
Room: Longs/Quandary Peaks

Speaker 2 of 4
Dasaradhi Palakodeti, Institute for Stem cell Biology and Regenerative Medicine, India
Small RNAs in the Planarian Schmidtea mediterranea

Monday, January 18 | 5:00PM - 7:00PM
Small RNAs in Stem Cells and Germ Cells
Room: Longs/Quandary Peaks

Speaker 3 of 4
Colin C. Conine, University of Massachusetts Medical School, USA
Short Talk: The Ago-Class Argonautes TZK-1 and TZK-2 Bind 26G-RNAs to Promote Male Fertility in C. elegans

Monday, January 18 | 5:00PM - 7:00PM
Small RNAs in Stem Cells and Germ Cells
Room: Longs/Quandary Peaks

Speaker 4 of 4
Alex Bortvin, Carnegie Institution of Washington, USA
Short Talk: Maelstrom and Mammalian Meiosis

Monday, January 18 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys Peaks


Monday, January 18 | 8:00PM - 11:00PM
Entertainment
Room: Shavano/Torreys Peaks


Monday, January 18 | 8:00PM - 11:00PM
Cash Bar
Room: Shavano/Torreys Peaks


Tuesday, January 19 | 10:25AM - 10:25AM
Departure


*Session Chair.