Web Desc
Dynamics of Eukaryotic Transcription during Development
Organizer(s): Karen Adelman and Marc Timmers
Date: April 07 - 12, 2010
Location: Big Sky Resort, Big Sky, MT, USA
Supported by The Directors' Fund
Summary of Meeting:
Gene expression in eukaryotes is regulated primarily at the level of transcription. Although much is known about the basic building blocks required to recruit RNA polymerase II to a gene promoter and establish a pre-initiation complex, recent data reveals that a great deal of regulation occurs during transcription elongation, and involves dynamic interactions between the transcription machinery and chromatin structure. This meeting is intended to explore these recently appreciated mechanisms for gene regulation and to showcase the novel techniques that have permitted these advances. Of particular interest are the use of comprehensive, genomic approaches, and their use towards understanding the different patterns of gene expression in different model systems, including stem cells and developing tissues, as well as the application of novel molecular probing and cell imaging techniques to investigate the global nuclear architecture and its impact on gene expression.
Scholarship Deadline: December 7 2009
Discounted Abstract Deadline: December 7 2009
Abstract Deadline: January 5 2010
Discounted Registration Deadline: February 5 2010
We gratefully acknowledge additional support for this conference from:
Development, The Company of Biologists LtdIntegrated DNA Technologies
National Institute of Environmental Health Sciences

National Institute of General Medical Sciences (NIGMS)
We gratefully acknowledge the generous grant for this conference provided by:

National Cancer Institute (NCI)
Grant No. 1R13CA150313-01
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Wednesday, April 07 | 3:00PM - 7:30PM
Registration
Room: Lower Atrium


Wednesday, April 07 | 6:30PM - 7:30PM
Refreshments
Room: Lower Atrium


Wednesday, April 07 | 7:30PM - 8:30PM
Keynote Address
Room: Gallatin

Speaker 2 of 2
Richard A. Young, Whitehead Institute for Biomedical Research, USA
Programming Embryonic Stem Cell State

Wednesday, April 07 | 7:30PM - 8:30PM
Keynote Address
Room: Gallatin

Speaker 1 of 2
* Karen Adelman, Harvard Medical School, USA

Thursday, April 08 | 7:00AM - 8:00AM
Breakfast
Room: Jefferson/Madison


Thursday, April 08 | 8:00AM - 11:00AM
Genomic Approaches to Understanding Gene Expression
Room: Gallatin

Speaker 6 of 6
W. Lee Kraus, University of Texas Southwestern Medical Center, USA
Short Talk: Exploring the Direct Estrogen-Regulated Transcriptome in Breast Cancer Cells Using GRO-Seq

Thursday, April 08 | 8:00AM - 11:00AM
Genomic Approaches to Understanding Gene Expression
Room: Gallatin

Speaker 5 of 6
Frank C.P. Holstege, University Medical Center, Utrecht, Netherlands
Understanding Regulatory Circuitry through Expression-Profile Phenotypes

Thursday, April 08 | 8:00AM - 11:00AM
Genomic Approaches to Understanding Gene Expression
Room: Gallatin

Speaker 4 of 6
B. Franklin Pugh, Pennsylvania State University, USA
Global Interplay between the Transcription Machinery and Chromatin

Thursday, April 08 | 8:00AM - 11:00AM
Genomic Approaches to Understanding Gene Expression
Room: Gallatin

Speaker 3 of 6
Steven Henikoff, Fred Hutchinson Cancer Research Center, USA
Mapping Genome-Wide Nucleosome Dynamics

Thursday, April 08 | 8:00AM - 11:00AM
Genomic Approaches to Understanding Gene Expression
Room: Gallatin

Speaker 2 of 6
Jonathan Widom, Northwestern University, USA
DNA-Encoded Nucleosome Organization of Eukaryotic Genomes

Thursday, April 08 | 8:00AM - 11:00AM
Genomic Approaches to Understanding Gene Expression
Room: Gallatin

Speaker 1 of 6
* Marc Timmers, University Medical Centre Utrecht, Netherlands

Thursday, April 08 | 9:20AM - 9:40AM
Coffee Break
Room: Lower Atrium


Thursday, April 08 | 11:00AM - 1:00PM
Poster Setup
Room: Jefferson/Madison


Thursday, April 08 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Thursday, April 08 | 1:00PM - 10:00PM
Poster Viewing
Room: Jefferson/Madison


Thursday, April 08 | 4:30PM - 5:00PM
Coffee Available
Room: Lower Atrium


Thursday, April 08 | 5:00PM - 7:00PM
Transcription Regulation in Stem Cells and during Developmen
t

Room: Gallatin

Speaker 5 of 5
X. Shirley Liu, Dana-Farber Cancer Institute, USA
Short Talk: Using Histone Mark Dynamics to Infer the Transcription Regulatory Network in Intestine Development

Thursday, April 08 | 5:00PM - 7:00PM
Transcription Regulation in Stem Cells and during Developmen
t

Room: Gallatin

Speaker 4 of 5
Kenneth S. Zaret, University of Pennsylvania, USA
Pioneer Transcription Factors in Programming of Liver and Pancreas Progenitors: Lessons for Stem Cell Differentiation

Thursday, April 08 | 5:00PM - 7:00PM
Transcription Regulation in Stem Cells and during Developmen
t

Room: Gallatin

Speaker 3 of 5
Fiona M. Watt, King's College London School of Medicine, UK
Proliferation and Differentiation of Epidermal Stem Cells


Thursday, April 08 | 5:00PM - 7:00PM
Transcription Regulation in Stem Cells and during Developmen
t

Room: Gallatin

Speaker 2 of 5
Michael S. Levine, Princeton University, USA
Gene Activation during Development and Differentiation in Drosophila

Thursday, April 08 | 5:00PM - 7:00PM
Transcription Regulation in Stem Cells and during Developmen
t

Room: Gallatin

Speaker 1 of 5
* Richard A. Young, Whitehead Institute for Biomedical Research, USA

Thursday, April 08 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Jefferson/Madison


Thursday, April 08 | 7:30PM - 10:00PM
Poster Session 1
Room: Jefferson/Madison


Friday, April 09 | 7:00AM - 8:00AM
Breakfast
Room: Jefferson/Madison


Friday, April 09 | 8:00AM - 11:00AM
Transcription Regulation - From Pol II Recruitment to Early
Elongation

Room: Gallatin

Speaker 1 of 6
* Kenneth S. Zaret, University of Pennsylvania, USA

Friday, April 09 | 8:00AM - 11:00AM
Transcription Regulation - From Pol II Recruitment to Early
Elongation

Room: Gallatin

Speaker 2 of 6
Robert G. Roeder, Rockefeller University, USA
Function of Diverse Transcriptional Coactivators in Animal Cells

Friday, April 09 | 8:00AM - 11:00AM
Transcription Regulation - From Pol II Recruitment to Early
Elongation

Room: Gallatin

Speaker 3 of 6
Marc Timmers, University Medical Centre Utrecht, Netherlands
Dynamic Regulation of the Basal Transcription Machinery

Friday, April 09 | 8:00AM - 11:00AM
Transcription Regulation - From Pol II Recruitment to Early
Elongation

Room: Gallatin

Speaker 4 of 6
Jennifer F. Kugel, University of Colorado Boulder, USA
Regulation of Early Steps of Transcription by ncRNAs

Friday, April 09 | 8:00AM - 11:00AM
Transcription Regulation - From Pol II Recruitment to Early
Elongation

Room: Gallatin

Speaker 5 of 6
Bijan Sobhian, Institute of Human Genetics, France
Short Talk: HIV-1 Tat Assembles a Multifunctional Transcription Elongation Complex and Stably Associates with the 7SK snRNP

Friday, April 09 | 8:00AM - 11:00AM
Transcription Regulation - From Pol II Recruitment to Early
Elongation

Room: Gallatin

Speaker 6 of 6
Laszlo Tora, Institut de Genetique et de Biologie Moleculaire et Cellulaire, France
TBP2 is Essential for Germ Cell Development by Regulating Transcription and Chromatin Condensation in the Oocyte

Friday, April 09 | 9:20AM - 9:40AM
Coffee Break
Room: Lower Atrium


Friday, April 09 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Friday, April 09 | 11:00AM - 1:00PM
Poster Setup
Room: Jefferson/Madison


Friday, April 09 | 1:00PM - 10:00PM
Poster Viewing
Room: Jefferson/Madison


Friday, April 09 | 2:30PM - 4:30PM
Workshop 1: Genomic Approaches: Discovery of Transcription M
odulators as Drug Targets and High-Throughput Techniques for Investigating Changes in Gene Expression
Room: Gallatin
This workshop is designed to engage members of pharmaceutical industry using new techniques to identify transcription inhibitors, as well as companies that do high-throughput sequencing.
Speaker 1 of 7
* Laszlo Tora, Institut de Genetique et de Biologie Moleculaire et Cellulaire, France

Friday, April 09 | 2:30PM - 4:30PM
Workshop 1: Genomic Approaches: Discovery of Transcription M
odulators as Drug Targets and High-Throughput Techniques for Investigating Changes in Gene Expression
Room: Gallatin
This workshop is designed to engage members of pharmaceutical industry using new techniques to identify transcription inhibitors, as well as companies that do high-throughput sequencing.
Speaker 2 of 7
James E. Bradner, Novartis Institutes for BioMedical Research, USA
Direct Inhibition of the Notch Transcription Factor Complex

Friday, April 09 | 2:30PM - 4:30PM
Workshop 1: Genomic Approaches: Discovery of Transcription M
odulators as Drug Targets and High-Throughput Techniques for Investigating Changes in Gene Expression
Room: Gallatin
This workshop is designed to engage members of pharmaceutical industry using new techniques to identify transcription inhibitors, as well as companies that do high-throughput sequencing.
Speaker 3 of 7
Jun Zhu, NHLBI, National Institutes of Health, USA
The Landscape of Transcription Initiation in the Drosophila melanogaster Embryo

Friday, April 09 | 2:30PM - 4:30PM
Workshop 1: Genomic Approaches: Discovery of Transcription M
odulators as Drug Targets and High-Throughput Techniques for Investigating Changes in Gene Expression
Room: Gallatin
This workshop is designed to engage members of pharmaceutical industry using new techniques to identify transcription inhibitors, as well as companies that do high-throughput sequencing.
Speaker 4 of 7
Tae-Kyung Kim, University of Texas Southwestern Medical Center, USA
Widespread Transcription at Thousands of Enhancers during Activity-Dependent Gene Expression in Neurons

Friday, April 09 | 2:30PM - 4:30PM
Workshop 1: Genomic Approaches: Discovery of Transcription M
odulators as Drug Targets and High-Throughput Techniques for Investigating Changes in Gene Expression
Room: Gallatin
This workshop is designed to engage members of pharmaceutical industry using new techniques to identify transcription inhibitors, as well as companies that do high-throughput sequencing.
Speaker 5 of 7
Marta Radman-Livaja, University of Massachusetts Medical School, USA
Gene Inactivation and Epigenetic Inheritance

Friday, April 09 | 2:30PM - 4:30PM
Workshop 1: Genomic Approaches: Discovery of Transcription M
odulators as Drug Targets and High-Throughput Techniques for Investigating Changes in Gene Expression
Room: Gallatin
This workshop is designed to engage members of pharmaceutical industry using new techniques to identify transcription inhibitors, as well as companies that do high-throughput sequencing.
Speaker 6 of 7
Michael P. Schnetz, Case Western Reserve University, USA
CHD7 Targets Active Gene Enhancer Elements to Modulate ES Cell-Specific Gene Expression

Friday, April 09 | 2:30PM - 4:30PM
Workshop 1: Genomic Approaches: Discovery of Transcription M
odulators as Drug Targets and High-Throughput Techniques for Investigating Changes in Gene Expression
Room: Gallatin
This workshop is designed to engage members of pharmaceutical industry using new techniques to identify transcription inhibitors, as well as companies that do high-throughput sequencing.
Speaker 7 of 7
Michael D. Wilson, SickKids Research Institute, Canada
Five Vertebrate ChIP-seq Reveals the Genetic Mechanisms of Rapid Transcription Factor Binding Divergence

Friday, April 09 | 4:30PM - 5:00PM
Coffee Available
Room: Lower Atrium


Friday, April 09 | 5:00PM - 7:00PM
Regulation of Transcription Elongation
Room: Gallatin

Speaker 1 of 5
* John T. Lis, Cornell University, USA

Friday, April 09 | 5:00PM - 7:00PM
Regulation of Transcription Elongation
Room: Gallatin

Speaker 2 of 5
Hiroshi Handa, Tokyo Institute of Technology, Japan
The Role of Transcription Elongation Factors in Genome Expression

Friday, April 09 | 5:00PM - 7:00PM
Regulation of Transcription Elongation
Room: Gallatin

Speaker 3 of 5
Karen Adelman, Harvard Medical School, USA
Pol II Stalling Across the Genome

Friday, April 09 | 5:00PM - 7:00PM
Regulation of Transcription Elongation
Room: Gallatin

Speaker 4 of 5
David S. Gilmour, Pennsylvania State University, USA
Biochemical and in vivo Analysis of Promoter Proximal Pausing

Friday, April 09 | 5:00PM - 7:00PM
Regulation of Transcription Elongation
Room: Gallatin

Speaker 5 of 5
L. Ryan Baugh, Duke University, USA
Short Talk: Nutritional Control of Pol II Pausing during C. elegans Development

Friday, April 09 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Jefferson/Madison


Friday, April 09 | 7:30PM - 10:00PM
Poster Session 2
Room: Jefferson/Madison


Saturday, April 10 | 7:00AM - 8:00AM
Breakfast
Room: Jefferson/Madison


Saturday, April 10 | 8:00AM - 11:00AM
The Interplay between Transcription and Chromatin
Room: Gallatin

Speaker 1 of 6
* Steven Henikoff, Fred Hutchinson Cancer Research Center, USA

Saturday, April 10 | 8:00AM - 11:00AM
The Interplay between Transcription and Chromatin
Room: Gallatin

Speaker 2 of 6
Jane Mellor, University of Oxford, UK
Histone Modifications, Transcription Factors, ncRNA and Gene Loops in Transcription

Saturday, April 10 | 8:00AM - 11:00AM
The Interplay between Transcription and Chromatin
Room: Gallatin

Speaker 3 of 6
Marc D. Meneghini, University of Toronto, Canada
Short Talk: Global Histone Demethylation Controls Spore Differentiation Timing during Budding Yeast Gametogenesis

Saturday, April 10 | 8:00AM - 11:00AM
The Interplay between Transcription and Chromatin
Room: Gallatin

Speaker 4 of 6
Olivier Cuvier, LBME- CNRS, France
Deciphering the Discrete Stages of Insulator-encoded Nucleosome-Positioning: The Road toward Transcriptional Competence

Saturday, April 10 | 8:00AM - 11:00AM
The Interplay between Transcription and Chromatin
Room: Gallatin

Speaker 5 of 6
Ali Shilatifard, Northwestern University, USA
Transcriptional Elongation Control and Human Leukemia

Saturday, April 10 | 8:00AM - 11:00AM
The Interplay between Transcription and Chromatin
Room: Gallatin

Speaker 6 of 6
David H. Price, University of Iowa, USA
Mechanism of Release of P-TEFb and HEXIM1 from the 7SK snRNP by HIV Tat and the Chromatin Binding Protein, Brd4

Saturday, April 10 | 9:20AM - 9:40AM
Coffee Break
Room: Lower Atrium


Saturday, April 10 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Saturday, April 10 | 11:00AM - 1:00PM
Poster Setup
Room: Jefferson/Madison


Saturday, April 10 | 1:00PM - 10:00PM
Poster Viewing
Room: Jefferson/Madison


Saturday, April 10 | 2:30PM - 4:30PM
Workshop 2: New and Noteworthy
Room: Gallatin

Speaker 1 of 7
* Joan W. Conaway, Stowers Institute for Medical Research, USA

Saturday, April 10 | 2:30PM - 4:30PM
Workshop 2: New and Noteworthy
Room: Gallatin

Speaker 2 of 7
Maria Ciofani, Skirball Institute of Biomolecular Medicine, USA
Transcriptional Regulatory Network Underlying CD4+ T Helper 17 (Th17) Lineage Specification

Saturday, April 10 | 2:30PM - 4:30PM
Workshop 2: New and Noteworthy
Room: Gallatin

Speaker 3 of 7
Suming Huang, Pennsylvania State University, USA
Serine Phosphorylation on TAL1 Regulates its Interaction with Histone Demethylase LSD1 during Hematopoiesis

Saturday, April 10 | 2:30PM - 4:30PM
Workshop 2: New and Noteworthy
Room: Gallatin

Speaker 4 of 7
Miguel Garcia-Diaz, Stony Brook University, USA
A Unique DNA Binding Mode Enables Human mterf1 to Regulate Mitochondrial Transcription

Saturday, April 10 | 2:30PM - 4:30PM
Workshop 2: New and Noteworthy
Room: Gallatin

Speaker 5 of 7
Akihiko Yokoyama, Kyoto University, Japan
Mechanisms of MLL-Dependent Transcriptional Maintenance in Normal and Cancer Cells

Saturday, April 10 | 2:30PM - 4:30PM
Workshop 2: New and Noteworthy
Room: Gallatin

Speaker 6 of 7
Madeline M. Wong, NIAID, NIH, USA
Promoter Bound p300 Complexes Facilitate Transmission of Molecular Memory Across the Cell Cycle

Saturday, April 10 | 2:30PM - 4:30PM
Workshop 2: New and Noteworthy
Room: Gallatin

Speaker 7 of 7
Ines de Santiago, Medical Research Council - Clinical Sciences Centre, UK
Genome-Wide Mapping of RNA Polymerase II Phosphorylation Elucidates the CTD Code in Embryonic Stem Cells

Saturday, April 10 | 4:30PM - 5:00PM
Coffee Available
Room: Lower Atrium


Saturday, April 10 | 5:00PM - 7:00PM
Activators and Co-Regulatory Complexes
Room: Gallatin

Speaker 1 of 5
* † Katherine A. Jones, The Salk Institute, USA

Saturday, April 10 | 5:00PM - 7:00PM
Activators and Co-Regulatory Complexes
Room: Gallatin

Speaker 2 of 5
Barbara Panning, University of California, San Francisco, USA
Tip60-p400 as a Regulator of Embryonic Stem Cell Identity

Saturday, April 10 | 5:00PM - 7:00PM
Activators and Co-Regulatory Complexes
Room: Gallatin

Speaker 3 of 5
Joan W. Conaway, Stowers Institute for Medical Research, USA
Mechanisms for Recruiting Chromatin Remodeling Complexes to Their Targets

Saturday, April 10 | 5:00PM - 7:00PM
Activators and Co-Regulatory Complexes
Room: Gallatin

Speaker 4 of 5
Susan E. Mango, Harvard University, USA
Investigating the Transcriptional Mechanisms that Govern the Progression of C. elegans Development

Saturday, April 10 | 5:00PM - 7:00PM
Activators and Co-Regulatory Complexes
Room: Gallatin

Speaker 5 of 5
Michael Meisterernst, Institute of Molecular Tumor Biology, Germany
Short Talk: Transcription Cofactor PC4 is Critical for Cellular Proliferation and Early Development of Mammals

Saturday, April 10 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Jefferson/Madison


Saturday, April 10 | 7:30PM - 10:00PM
Poster Session 3
Room: Jefferson/Madison


Sunday, April 11 | 7:00AM - 8:00AM
Breakfast
Room: Jefferson/Madison


Sunday, April 11 | 8:00AM - 11:00AM
Co-Transcriptional RNA Processing and Non-Coding RNAs
Room: Gallatin

Speaker 1 of 5
* Wendy A. Bickmore, University of Edinburgh, UK

Sunday, April 11 | 8:00AM - 11:00AM
Co-Transcriptional RNA Processing and Non-Coding RNAs
Room: Gallatin

Speaker 2 of 5
Stephen Buratowski, Harvard Medical School, USA
Connecting Transcription to Chromatin and mRNA Processing

Sunday, April 11 | 8:00AM - 11:00AM
Co-Transcriptional RNA Processing and Non-Coding RNAs
Room: Gallatin

Speaker 3 of 5
Katherine A. Jones, The Salk Institute, USA
SKIP, a Cell Survival Factor that Controls Elongation and Gene-Specific Splicing

Sunday, April 11 | 8:00AM - 11:00AM
Co-Transcriptional RNA Processing and Non-Coding RNAs
Room: Gallatin

Speaker 4 of 5
Sukesh R. Bhaumik, Southern Illinois University School of Medicine, USA
Short Talk: A Novel Regulatory Pathway of Eukaryotic Transcriptional Activation by mRNA Cap-Binding Complex in vivo

Sunday, April 11 | 8:00AM - 11:00AM
Co-Transcriptional RNA Processing and Non-Coding RNAs
Room: Gallatin

Speaker 5 of 5
Ramin Shiekhattar, University of Miami, USA
Elucidating the Function of Long Non-Coding RNAs

Sunday, April 11 | 9:20AM - 9:40AM
Coffee Break
Room: Lower Atrium


Sunday, April 11 | 4:30PM - 5:00PM
Coffee Available
Room: Lower Atrium


Sunday, April 11 | 5:00PM - 7:00PM
Imaging and Probing Global Nuclear Architecture
Room: Gallatin

Speaker 1 of 5
* Stephen Buratowski, Harvard Medical School, USA

Sunday, April 11 | 5:00PM - 7:00PM
Imaging and Probing Global Nuclear Architecture
Room: Gallatin

Speaker 2 of 5
Bas van Steensel, Netherlands Cancer Institute, Netherlands
Architecture and Function of Genome - Nuclear Lamina Interactions

Sunday, April 11 | 5:00PM - 7:00PM
Imaging and Probing Global Nuclear Architecture
Room: Gallatin

Speaker 3 of 5
Wendy A. Bickmore, University of Edinburgh, UK
Ring1B Compacts Chromatin Structure, and Represses Gene Expression, Independent of Histone Ubiquitination

Sunday, April 11 | 5:00PM - 7:00PM
Imaging and Probing Global Nuclear Architecture
Room: Gallatin

Speaker 4 of 5
Jie Yao, Yale University School of Medicine, USA
Short Talk: Coordinate Re-Positioning of Genes and Core Promoter Selectivity Factors during Myogenesis

Sunday, April 11 | 5:00PM - 7:00PM
Imaging and Probing Global Nuclear Architecture
Room: Gallatin

Speaker 5 of 5
John T. Lis, Cornell University, USA
New Views of Local and Genome-Wide Transcription Activation in Vivo

Sunday, April 11 | 7:00PM - 7:15PM
Closing Remarks

Speaker 1 of 1
Marc Timmers, University Medical Centre Utrecht, Netherlands
Closing Remarks

Sunday, April 11 | 7:15PM - 8:15PM
Social Hour with Lite Bites
Room: Jefferson/Madison


Sunday, April 11 | 8:00PM - 11:00PM
Entertainment
Room: Jefferson/Madison


Sunday, April 11 | 8:00PM - 11:00PM
Cash Bar
Room: Jefferson/Madison


Monday, April 12 | 10:25AM - 10:25AM
Departure


*Session Chair.