Web Desc
AAA+ and Related ATP-Driven Protein Machines: Structure, Function and Mechanism
Organizer(s): Andreas Matouschek, Robert T. Sauer and Phyllis I. Hanson
Date: March 20 - 24, 2011
Location: Granlibakken Tahoe, Tahoe City, CA, USA
Sponsored by Bayer USA Foundation
Summary of Meeting:
AAA+ protein machines catalyze basic biological processes: they degrade proteins, remodel protein complexes and nucleic acids, transport macromolecules across membranes, and shape membranes. They are at the center of cellular processes such as cell cycle control, protein sorting, DNA replication, cytoskeleton dynamics, and membrane budding. Thus, AAA+ machines play roles in a wide range of disease including cancer, neurodegeneration and infection. Despite the diversity of function, there appears to be a common operating mode of AAA+ proteins and we are at the cusp of acquiring a deep understanding of their molecular mechanism. However, different aspects of the mechanism are understood in different systems. At this conference we bring together speakers who have elucidated critical aspects of different AAA+ proteins. The group transcends molecular scales and methodology. We add a smaller number of speakers who have made breakthroughs in the understanding of proteins that do not belong to the AAA+ family but are either functionally or structurally related. We aniticipate that this combination will lead to insights into the common mechanism of these proteins and inspire novel experimental approaches and discoveries.
Scholarship Deadline: November 18 2010
Discounted Abstract Deadline: November 18 2010
Abstract Deadline: December 21 2010
Discounted Registration Deadline: January 20 2011
We gratefully acknowledge additional support for this conference from:
Bayer USA Foundation
We gratefully acknowledge the generous grant for this conference provided by:

National Science Foundation (NSF)
Grant No. MCB-1112682
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Sunday, March 20 | 3:00PM - 7:30PM
Registration
Room: Pre Function


Sunday, March 20 | 7:15PM - 8:30PM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 1 of 4
* Andreas Matouschek, University of Texas at Austin, USA

Sunday, March 20 | 7:15PM - 8:30PM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 2 of 4
* Bob T. Sauer, Massachusetts Institute of Technology, USA

Sunday, March 20 | 7:15PM - 8:30PM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 3 of 4
* Phyllis I. Hanson, University of Michigan, USA

Sunday, March 20 | 7:15PM - 8:30PM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 4 of 4
Tania A. Baker, Massachusetts Institute of Technology, USA
AAA+ Proteases in Protein Quality Control

Monday, March 21 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Monday, March 21 | 8:00AM - 11:00AM
AAA Proteases and Remodeling
Room: Mountain/Lake

Speaker 1 of 7
* Bob T. Sauer, Massachusetts Institute of Technology, USA
The ClpXP Protein-Unfolding Machine

Monday, March 21 | 8:00AM - 11:00AM
AAA Proteases and Remodeling
Room: Mountain/Lake

Speaker 2 of 7
Aaron L. Lucius, University of Alabama at Birmingham, USA
Short Talk: Examination of E. coli ClpA Catalyzed Polypeptide Translocation Using Single-Turnover Fluorescence Stopped-Flow Techniques

Monday, March 21 | 8:00AM - 11:00AM
AAA Proteases and Remodeling
Room: Mountain/Lake

Speaker 3 of 7
Michael Maurizi, National Cancer Institute, USA
Short Talk: Molecular Architecture of Lon Protease: Gated Access to a Sequestered Degradation Chamber

Monday, March 21 | 8:00AM - 11:00AM
AAA Proteases and Remodeling
Room: Mountain/Lake

Speaker 4 of 7
Francis T.F. Tsai, Baylor College of Medicine, USA
Short Talk: Structure and Mechanism of the Hsp104 AAA+ ATPase

Monday, March 21 | 8:00AM - 11:00AM
AAA Proteases and Remodeling
Room: Mountain/Lake

Speaker 5 of 7
James Shorter, University of Pennsylvania, USA
Short Talk: Hsp104: Structure, Mechanism and Functional Applications

Monday, March 21 | 8:00AM - 11:00AM
AAA Proteases and Remodeling
Room: Mountain/Lake

Speaker 6 of 7
Joel R. Hoskins, National Cancer Institute, USA
Short Talk: Species-Specific Cooperation of Hsp104/ClpB and Hsp70/DnaK in Thermotolerance and Protein Disaggregation is Dictated by the M-Domain of Hsp104/ClpB

Monday, March 21 | 8:00AM - 11:00AM
AAA Proteases and Remodeling
Room: Mountain/Lake

Speaker 7 of 7
Eilika Weber-Ban, ETH Zürich, Switzerland
Short Talk: Roles of the AAA ATPase Mpa in the Pup Proteasome System

Monday, March 21 | 9:20AM - 9:40AM
Coffee Break
Room: Pre Function


Monday, March 21 | 11:00AM - 1:00PM
Poster Setup
Room: Bay


Monday, March 21 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Monday, March 21 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Monday, March 21 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Monday, March 21 | 5:00PM - 7:15PM
Proteasome and p97: Targeting Proteins to AAA Machines
Room: Mountain/Lake

Speaker 1 of 4
Nieng Yan, Princeton University, USA
Structure of the CED-4 Apoptosome: Insights into its Assembly and Function

Monday, March 21 | 5:00PM - 7:15PM
Proteasome and p97: Targeting Proteins to AAA Machines
Room: Mountain/Lake

Speaker 2 of 4
Daniel J. Finley, Harvard University, USA
Assembly and Function of the Hetero-Hexameric AAA Ring of the Proteasome

Monday, March 21 | 5:00PM - 7:15PM
Proteasome and p97: Targeting Proteins to AAA Machines
Room: Mountain/Lake

Speaker 3 of 4
* Raymond J. Deshaies, Amgen, Inc., USA
p97 as a Protein Targeting Platform

Monday, March 21 | 5:00PM - 7:15PM
Proteasome and p97: Targeting Proteins to AAA Machines
Room: Mountain/Lake

Speaker 4 of 4
Andreas Matouschek, University of Texas at Austin, USA
A Second Component to the Degradation Signal: The Proteasome Initiation Site

Monday, March 21 | 7:15PM - 8:15PM
Dinner
Room: Granhall


Monday, March 21 | 8:00PM - 10:00PM
Poster Session 1
Room: Bay


Monday, March 21 | 8:00PM - 9:00PM
Social Hour
Room: Bay


Tuesday, March 22 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Tuesday, March 22 | 8:00AM - 11:00AM
DNA Gymnastics
Room: Mountain/Lake

Speaker 1 of 5
* James M. Berger, Johns Hopkins University School of Medicine, USA
Structural Basis for Hexameric Helicase Translocation and Polarity

Tuesday, March 22 | 8:00AM - 11:00AM
DNA Gymnastics
Room: Mountain/Lake

Speaker 2 of 5
Michael Botchan, University of California, Berkeley, USA
Adaptors Essential for the Activity of the MCM Helicase

Tuesday, March 22 | 8:00AM - 11:00AM
DNA Gymnastics
Room: Mountain/Lake

Speaker 3 of 5
Stephen C. Kowalczykowski, University of California, Davis, USA
Visualization of DNA Helicases and DNA Strand Exchange Proteins at the Single-Molecule Level

Tuesday, March 22 | 8:00AM - 11:00AM
DNA Gymnastics
Room: Mountain/Lake

Speaker 4 of 5
Brian A. Kelch, University of California Berkeley, USA
Short Talk: Crystal Structures Provide Snapshots of the Clamp Loading Mechanism

Tuesday, March 22 | 8:00AM - 11:00AM
DNA Gymnastics
Room: Mountain/Lake

Speaker 5 of 5
Taekjip Ha, Johns Hopkins University, USA
Reverse-Chaperoning Activity of an AAA+ Protein

Tuesday, March 22 | 9:20AM - 9:40AM
Coffee Break
Room: Pre Function


Tuesday, March 22 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Tuesday, March 22 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Tuesday, March 22 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Tuesday, March 22 | 5:00PM - 7:00PM
DNA Gymnastics II and Moving Molecules Across Membranes
Room: Mountain/Lake

Speaker 1 of 4
Xiaodong Zhang, Imperial College London, UK
Structures and Mechanism of AAA+ Transcriptional Activators

Tuesday, March 22 | 5:00PM - 7:00PM
DNA Gymnastics II and Moving Molecules Across Membranes
Room: Mountain/Lake

Speaker 2 of 4
Tatyana A. Sysoeva, Pennsylvania State University, USA
Short Talk: Intricate Interactions among Subunits within the Ring of an AAA+ ATPase

Tuesday, March 22 | 5:00PM - 7:00PM
DNA Gymnastics II and Moving Molecules Across Membranes
Room: Mountain/Lake

Speaker 3 of 4
Jorge E. Galán, Yale University School of Medicine, USA
Type III Secretion Machines: Bacterial Devices for Protein Delivery into Eukaryotic Cells

Tuesday, March 22 | 5:00PM - 7:00PM
DNA Gymnastics II and Moving Molecules Across Membranes
Room: Mountain/Lake

Speaker 4 of 4
* Briana M. Burton, Harvard University, USA
Importing DNA and Exporting Proteins

Tuesday, March 22 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Tuesday, March 22 | 8:00PM - 10:00PM
Poster Viewing
Room: Bay


Tuesday, March 22 | 8:00PM - 9:00PM
Social Hour
Room: Bay


Wednesday, March 23 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Wednesday, March 23 | 8:00AM - 11:00AM
Building and Rebuilding the Cytoskeleton and Other Interesti
ng ATPases

Room: Mountain/Lake

Speaker 1 of 4
Ronald D. Vale, University of California, San Francisco, USA
The Structure of the Dynein Motor

Wednesday, March 23 | 8:00AM - 11:00AM
Building and Rebuilding the Cytoskeleton and Other Interesti
ng ATPases

Room: Mountain/Lake

Speaker 2 of 4
* Eva Nogales, University of California, Berkeley, USA
Visualizing the Regulated, Dynamic Interaction of Microtubules with Kinetochores

Wednesday, March 23 | 8:00AM - 11:00AM
Building and Rebuilding the Cytoskeleton and Other Interesti
ng ATPases

Room: Mountain/Lake

Speaker 3 of 4
Antonina Roll-Mecak, DHHS/NINDS, National Institutes of Health, USA
Cytoskeleton Regulation by AAA+ Proteins: Molecular Dissection of Microtubule Severing

Wednesday, March 23 | 8:00AM - 11:00AM
Building and Rebuilding the Cytoskeleton and Other Interesti
ng ATPases

Room: Mountain/Lake

Speaker 4 of 4
Michal Sharon, Weizmann Institute of Science, Israel
Short Talk: Characterizing the Cooperative Behavior of ATP Driven Machines

Wednesday, March 23 | 9:20AM - 9:40AM
Coffee Break
Room: Pre Function


Wednesday, March 23 | 11:00AM - 11:00AM
On Own for Lunch and Recreation


Wednesday, March 23 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Wednesday, March 23 | 5:00PM - 7:00PM
Shaping Membranes
Room: Mountain/Lake

Speaker 1 of 4
Wesley I. Sundquist, University of Utah School of Medicine, USA
Structure and Function of the VPS4 ATPase

Wednesday, March 23 | 5:00PM - 7:00PM
Shaping Membranes
Room: Mountain/Lake

Speaker 2 of 4
* Phyllis I. Hanson, University of Michigan, USA
VPS4, ESCRT-III, and Membrane Deformation

Wednesday, March 23 | 5:00PM - 7:00PM
Shaping Membranes
Room: Mountain/Lake

Speaker 3 of 4
James H. Hurley, University of California, Berkeley, USA
Its all in the Neck: How the ESCRTs Make Multivesicular Bodies

Wednesday, March 23 | 5:00PM - 7:00PM
Shaping Membranes
Room: Mountain/Lake

Speaker 4 of 4
Harish N. Ramanathan, YALE UNIVERSITY, USA
Short Talk: The p97 ATPase Associates with EEA1 to Regulate the Size of Early Endosomes

Wednesday, March 23 | 7:00PM - 7:15PM
Closing Remarks/Summary

Speaker 1 of 3
Andreas Matouschek, University of Texas at Austin, USA

Wednesday, March 23 | 7:00PM - 7:15PM
Closing Remarks/Summary

Speaker 2 of 3
Bob T. Sauer, Massachusetts Institute of Technology, USA

Wednesday, March 23 | 7:00PM - 7:15PM
Closing Remarks/Summary

Speaker 3 of 3
Phyllis I. Hanson, University of Michigan, USA

Wednesday, March 23 | 7:15PM - 8:15PM
Dinner
Room: Granhall


Wednesday, March 23 | 8:00PM - 11:00PM
Entertainment
Room: Granhall


Wednesday, March 23 | 8:00PM - 11:00PM
Cash Bar
Room: Granhall


Thursday, March 24 | 10:25AM - 10:25AM
Departure


*Session Chair.