Web Desc
The Ins and Outs of Viral Infection: Entry, Assembly, Exit and Spread
Organizer(s): Karla Kirkegaard, Mavis Agbandje-McKenna and Eric O. Freed
Date: March 30 - April 04, 2014
Location: Beaver Run Resort, Breckenridge, CO, USA
Sponsored by Astellas Pharma Inc.
Summary of Meeting:
Viruses can enter cells by mechanisms such as the classic virus-receptor interactions, cell-cell fusion and newly observed pathways that are currently being investigated. Similarly, viral exit from infected cells can follow the paradigms of cell lysis or viral budding, but new mechanisms such as unconventional secretion and exosome formation are emerging. Viruses in the process of entering or exiting cells should be especially vulnerable to antivirals because of their accessibility and because the formation of oligomeric structures that mix drug-susceptible and drug-resistant capsids can suppress the emergence of drug-resistant viruses. However, these steps are more difficult to target biochemically due to the lack of readily assayed enzymatic activity. In this meeting, we will bring together structural biologists, geneticists, cell biologists and mathematic modeling to address the mechanisms and consequences of the different modes of viral travel.
Scholarship Deadline: December 2 2013
Discounted Abstract Deadline: December 2 2013
Abstract Deadline: January 7 2014
Discounted Registration Deadline: January 29 2014
Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:
Astellas Pharma Inc.
We gratefully acknowledge the generous grant for this conference provided by:

National Institute of Allergy and Infectious Diseases (NIAID)
Grant No. 1R13AI109804-01
The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention of trade names, commercial practices, or organizations imply endorsement by the U.S. Government.
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

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Program
Sunday, March 30 | 4:00PM - 8:00PM
Arrival and Registration
Room: Foyer
Sunday, March 30 | 6:00PM - 8:00PM
Welcome Mixer
Room: Foyer
Monday, March 31 | 7:00AM - 8:00AM
Breakfast
Room: Summit Gallery
Monday, March 31 | 8:00AM - 9:00AM
Keynote Address
Room: Peaks 4-5

Speaker 1 of 2
* Karla A. Kirkegaard, Stanford University School of Medicine, USA
Monday, March 31 | 8:00AM - 9:00AM
Keynote Address
Room: Peaks 4-5

Speaker 2 of 2
Geoffrey L. Smith, University of Cambridge, UK
Vaccinia Virus Propulsion and Repulsion: A Portrait of a Poxvirus
Monday, March 31 | 9:00AM - 11:00AM
Mechanisms of Cell Entry I
Room: Peaks 4-5

Speaker 1 of 5
* Eric O. Freed, NCI, National Institutes of Health, USA
Monday, March 31 | 9:00AM - 11:00AM
Mechanisms of Cell Entry I
Room: Peaks 4-5

Speaker 2 of 5
Sean P. Whelan, Harvard Medical School, USA
VSV Entry
Monday, March 31 | 9:00AM - 11:00AM
Mechanisms of Cell Entry I
Room: Peaks 4-5

Speaker 3 of 5
Mavis Agbandje-McKenna, University of Florida, USA
Non-Enveloped Capsid Calisthenics
Monday, March 31 | 9:00AM - 11:00AM
Mechanisms of Cell Entry I
Room: Peaks 4-5

Speaker 4 of 5
Yasmine Baktash, University of Chicago, USA
Short Talk: Single Particle Tracking Analysis of Hepatitis C Virus Entry into Polarized Three-Dimensional Hepatoma Organoids
Monday, March 31 | 9:00AM - 11:00AM
Mechanisms of Cell Entry I
Room: Peaks 4-5

Speaker 5 of 5
Mathieu Mateo, Mayo Clinic, USA
Short Talk: Measles Virus Appropriates the Adhesive Surface of Nectins to Enter the Respiratory Epithelium
Monday, March 31 | 9:40AM - 10:00AM
Coffee Break
Room: Foyer
Monday, March 31 | 11:00AM - 1:00PM
Poster Setup
Room: Peaks 1-3
Monday, March 31 | 11:00AM - 2:30PM
On Own for Lunch and Recreation
Monday, March 31 | 1:00PM - 10:00PM
Poster Viewing
Room: Peaks 1-3
Monday, March 31 | 2:30PM - 4:30PM
Workshop 1: Entry
Room: Peaks 4-5

Speaker 1 of 6
Luk H. Vandenberghe, Harvard Medical School, USA
Ancestral Reconstruction of Adeno-Associated Virus Particles to Circumvent Pre-Existing Immunity
Monday, March 31 | 2:30PM - 4:30PM
Workshop 1: Entry
Room: Peaks 4-5

Speaker 2 of 6
* Colin R. Parrish, Cornell University, USA
Parvoviruses, Receptors and Antibodies – Host-Specific Interactions and their Controls
Monday, March 31 | 2:30PM - 4:30PM
Workshop 1: Entry
Room: Peaks 4-5

Speaker 3 of 6
Dennis T. Brown, North Carolina State University, USA
Alphavirus Entry
Monday, March 31 | 2:30PM - 4:30PM
Workshop 1: Entry
Room: Peaks 4-5

Speaker 4 of 6
Kathryn Twigg Arrildt, GlaxoSmithKline, USA
Features of Macrophage-Tropic HIV-1 Entry Phenotype
Monday, March 31 | 2:30PM - 4:30PM
Workshop 1: Entry
Room: Peaks 4-5

Speaker 5 of 6
Long Gui, University of Washington, USA
Membrane Deformations during Influenza Virus Fusion Imaged by Electron Cryo-Tomography
Monday, March 31 | 2:30PM - 4:30PM
Workshop 1: Entry
Room: Peaks 4-5

Speaker 6 of 6
Joseph R. Pasquarella, University of Wisconsin, Madison, USA
Human Cytomegalovirus Enters Latency via Macropinocytosis
Monday, March 31 | 4:30PM - 5:00PM
Coffee Available
Room: Foyer
Monday, March 31 | 5:00PM - 7:15PM
Mechanisms of Cell Entry II
Room: Peaks 4-5

Speaker 1 of 6
* Julie K. Pfeiffer, University of Texas Southwestern Medical Center, USA
Monday, March 31 | 5:00PM - 7:15PM
Mechanisms of Cell Entry II
Room: Peaks 4-5

Speaker 2 of 6
Carolyn B. Coyne, University of Pittsburgh, USA
Three-Dimensional Cell Models for the Study of Virus Entry and Infection
Monday, March 31 | 5:00PM - 7:15PM
Mechanisms of Cell Entry II
Room: Peaks 4-5

Speaker 3 of 6
Matthew J. Evans, Icahn School of Medicine at Mount Sinai, USA
The Complexities of Hepatitis C Virus Cell Entry
Monday, March 31 | 5:00PM - 7:15PM
Mechanisms of Cell Entry II
Room: Peaks 4-5

Speaker 4 of 6
Jason Mercer, ETH Zürich, Switzerland
From Screen to Gene: Unraveling the Mystery of Poxvirus Genome Uncoating
Monday, March 31 | 5:00PM - 7:15PM
Mechanisms of Cell Entry II
Room: Peaks 4-5

Speaker 5 of 6
Ben Berkhout, University of Amsterdam, Academic Medical Center, Netherlands
Short Talk: HIV-1 Resistance and Dependence on Peptidic Fusion Inhibitors
Monday, March 31 | 5:00PM - 7:15PM
Mechanisms of Cell Entry II
Room: Peaks 4-5

Speaker 6 of 6
Shan-Lu Liu, Ohio State University, USA
Short Talk: Restriction of Viral Entry by Interferon-Induced Transmembrane Protein 1 (IFITM1)
Monday, March 31 | 7:15PM - 8:15PM
Social Hour with Lite Bites
Room: Peaks 1-3
Monday, March 31 | 7:30PM - 10:00PM
Poster Session 1
Room: Peaks 1-3
Tuesday, April 01 | 7:00AM - 8:00AM
Breakfast
Room: Summit Gallery
Tuesday, April 01 | 8:00AM - 11:00AM
Virion Assembly and Post-Assembly Maturation
Room: Peaks 4-5

Speaker 1 of 6
* Shan-Lu Liu, Ohio State University, USA
Tuesday, April 01 | 8:00AM - 11:00AM
Virion Assembly and Post-Assembly Maturation
Room: Peaks 4-5

Speaker 2 of 6
Andrea Gamarnik, Fundación Instituto Leloir, Argentina
Dengue Virus Capsid Functions: Encapsidation and Uncoating
Tuesday, April 01 | 8:00AM - 11:00AM
Virion Assembly and Post-Assembly Maturation
Room: Peaks 4-5

Speaker 3 of 6
Brett D. Lindenbach, Yale University School of Medicine, USA
An Insider’s Guide to an Outbound Virus: The Structure and Assembly of HCV Particles
Tuesday, April 01 | 8:00AM - 11:00AM
Virion Assembly and Post-Assembly Maturation
Room: Peaks 4-5

Speaker 4 of 6
John (Jack) E. Johnson, The Scripps Research Institute, USA
Virus Particle Maturation: Insights into Elegantly Programmed Nano Machines
Tuesday, April 01 | 8:00AM - 11:00AM
Virion Assembly and Post-Assembly Maturation
Room: Peaks 4-5

Speaker 5 of 6
Eric O. Freed, NCI, National Institutes of Health, USA
Recent Progress in Developing Potent and Broadly Active HIV-1 Maturation Inhibitors
Tuesday, April 01 | 8:00AM - 11:00AM
Virion Assembly and Post-Assembly Maturation
Room: Peaks 4-5

Speaker 6 of 6
Anastasia A. Aksyuk, NIAMS, National Institutes of Health, USA
Short Talk: Structural Studies of Herpes Simplex Virus 1 Procapsids and Maturation Intermediates
Tuesday, April 01 | 9:20AM - 9:40AM
Coffee Break
Room: Foyer
Tuesday, April 01 | 11:00AM - 1:00PM
Poster Setup
Room: Peaks 1-3
Tuesday, April 01 | 11:00AM - 5:00PM
On Own for Lunch and Recreation
Tuesday, April 01 | 1:00PM - 10:00PM
Poster Viewing
Room: Peaks 1-3
Tuesday, April 01 | 4:30PM - 5:00PM
Coffee Available
Room: Foyer
Tuesday, April 01 | 5:00PM - 7:15PM
Cell Exit I
Room: Peaks 4-5

Speaker 1 of 6
* Johnson Mak, Deakin University, Australia
Tuesday, April 01 | 5:00PM - 7:15PM
Cell Exit I
Room: Peaks 4-5

Speaker 2 of 6
Ryland F. Young, Texas A & M University, USA
Phage Lysis: The 'Hole' Story
Tuesday, April 01 | 5:00PM - 7:15PM
Cell Exit I
Room: Peaks 4-5

Speaker 3 of 6
Hyeryun Choe, The Scripps Research Institute, USA
Exiting to Enter: Phosphatidylserine and its Receptors in the Entry of Enveloped Viruses
Tuesday, April 01 | 5:00PM - 7:15PM
Cell Exit I
Room: Peaks 4-5

Speaker 4 of 6
Karla A. Kirkegaard, Stanford University School of Medicine, USA
Lytic and Non-Lytic Viral Spread
Tuesday, April 01 | 5:00PM - 7:15PM
Cell Exit I
Room: Peaks 4-5

Speaker 5 of 6
Maria Guadalupe Martinez, INSERM 1052 Centre de Recherche en Cancerologie, CNRS 5286, France
Short Talk: Imaging the Alphavirus Exit Pathway
Tuesday, April 01 | 5:00PM - 7:15PM
Cell Exit I
Room: Peaks 4-5

Speaker 6 of 6
Charles Grose, University of Iowa, USA
Short Talk: Autophagy Facilitates Cell-to-Cell Spread by Varicella-Zoster Virus
Tuesday, April 01 | 7:15PM - 8:15PM
Social Hour with Lite Bites
Room: Peaks 1-3
Tuesday, April 01 | 7:30PM - 10:00PM
Poster Session 2
Room: Peaks 1-3
Wednesday, April 02 | 7:00AM - 8:00AM
Breakfast
Room: Summit Gallery
Wednesday, April 02 | 8:00AM - 11:15AM
Virus Structure
Room: Peaks 4-5

Speaker 1 of 7
* Mavis Agbandje-McKenna, University of Florida, USA
Wednesday, April 02 | 8:00AM - 11:15AM
Virus Structure
Room: Peaks 4-5

Speaker 2 of 7
Johnson Mak, Deakin University, Australia
Structural Rearrangement during HIV Entry
Wednesday, April 02 | 8:00AM - 11:15AM
Virus Structure
Room: Peaks 4-5

Speaker 3 of 7
Z. Hong Zhou, University of California, Los Angeles, USA
A New Topology of the HK97-Like Fold Revealed in Bordetella Bacteriophage by cryoEM at 3.5Å Resolution
Wednesday, April 02 | 8:00AM - 11:15AM
Virus Structure
Room: Peaks 4-5

Speaker 4 of 7
Félix Rey, Institut Pasteur, France
Ancestral Genes Used for Virus-Cell and Cell-Cell Fusion Identified by Structural Studies
Wednesday, April 02 | 8:00AM - 11:15AM
Virus Structure
Room: Peaks 4-5

Speaker 5 of 7
John P. Moore, Weill Medical College of Cornell University, USA
The HIV-1 Env Trimer: The Key to Virus Entry and its Prevention by a Vaccine
Wednesday, April 02 | 8:00AM - 11:15AM
Virus Structure
Room: Peaks 4-5

Speaker 6 of 7
Wei Cheng, University of Michigan, USA
Short Talk: Optical Trapping and Multi-Parameter Analysis of Single HIV-1 in Culture Media Reveal the Positive Cooperativity of Envelope Spikes in Mediating Viral Infection
Wednesday, April 02 | 8:00AM - 11:15AM
Virus Structure
Room: Peaks 4-5

Speaker 7 of 7
Halil Aydin, University of Toronto, Canada
Short Talk: General Structural Features and Determinants Required for the Stabilization of Class I Viral Fusion Glycoproteins
Wednesday, April 02 | 9:20AM - 9:40AM
Coffee Break
Room: Foyer
Wednesday, April 02 | 11:15AM - 1:00PM
Poster Setup
Room: Peaks 1-3
Wednesday, April 02 | 11:15AM - 5:00PM
On Own for Lunch and Recreation
Wednesday, April 02 | 1:00PM - 10:00PM
Poster Viewing
Room: Peaks 1-3
Wednesday, April 02 | 4:30PM - 5:00PM
Coffee Available
Room: Foyer
Wednesday, April 02 | 5:00PM - 7:00PM
Cell Exit II
Room: Peaks 4-5

Speaker 1 of 6
* Akira Ono, University of Michigan, USA
Wednesday, April 02 | 5:00PM - 7:00PM
Cell Exit II
Room: Peaks 4-5

Speaker 2 of 6
Juan Martin-Serrano, King's College London School of Medicine, UK
Multiple Roles of the ESCRT Machinery in Viral Replication
Wednesday, April 02 | 5:00PM - 7:00PM
Cell Exit II
Room: Peaks 4-5

Speaker 3 of 6
Rebecca Dutch, University of Kentucky, USA
Human Metapneumovirus Assembly and Transmission
Wednesday, April 02 | 5:00PM - 7:00PM
Cell Exit II
Room: Peaks 4-5

Speaker 4 of 6
Katya E. Heldwein, Tufts University School of Medicine, USA
Short Talk: Membrane Deformation and Scission by the HSV-1 Nuclear Egress Complex
Wednesday, April 02 | 5:00PM - 7:00PM
Cell Exit II
Room: Peaks 4-5

Speaker 5 of 6
Keisuke Tabata, Research Institute for Microbial Diseases, Osaka University, Japan
Short Talk: Involvement of ESCRT Factors in Flavivirus Propagation
Wednesday, April 02 | 5:00PM - 7:00PM
Cell Exit II
Room: Peaks 4-5

Speaker 6 of 6
Jeremy Rossman, University of Kent, UK
Short Talk: M2-Mediated Influenza Virus Budding
Wednesday, April 02 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Peaks 1-3
Wednesday, April 02 | 7:30PM - 10:00PM
Poster Session 3
Room: Peaks 1-3
Thursday, April 03 | 7:00AM - 8:00AM
Breakfast
Room: Summit Gallery
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 1 of 8
* Andrew Mehle, University of Wisconsin, Madison, USA
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 2 of 8
Brenda G. Hogue, Arizona State University, USA
Role of Coronavirus Envelope (E) Viroporin in Assembly at Intracellular Membranes
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 3 of 8
Alasdair C. Steven, NIAMS, National Institutes of Health, USA
Genome Transport and Iron Storage: A Jack of Several Trades
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 4 of 8
Alan S. Perelson, Los Alamos National Laboratory, USA
Modeling Viral Spread
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 5 of 8
Erica Ollmann Saphire, La Jolla Institute for Immunology, USA
Structural Transformation Begets Multiple Functions in the Viral Life Cycle
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 6 of 8
Katharine G. Harris, University of Chicago, USA
Short Talk: Coxsackievirus B3 Cleaves Receptor-Interacting Protein Kinase 3 (RIP3) to alter Host Cell Death and Toll-Like Receptor Signaling
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 7 of 8
Roy Duncan, Dalhousie University, Canada
Short Talk: Reovirus FAST Proteins Usurp Host Exosome Biogenesis to Enhance Cell-Cell Fusion and Virus Dissemination
Thursday, April 03 | 8:00AM - 11:30AM
Virus Spread I
Room: Peaks 4-5

Speaker 8 of 8
Matthew P. Taylor, Montana State University, USA
Short Talk: Analysis of Neurotropic Virus Axon-to-Cell Spread
Thursday, April 03 | 9:20AM - 9:40AM
Coffee Break
Room: Foyer
Thursday, April 03 | 11:30AM - 2:30PM
On Own for Lunch and Recreation
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 1 of 8
* Sara W. Bird, uBiome, USA
Non-Lytic Viral Spread Enhanced by Autophagy Components
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 2 of 8
* Philip R. Tedbury, National Cancer Institute, USA
HIV-1 Envelope Glycoprotein Incorporation Requires Matrix Trimerization
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 3 of 8
Shu-Jung Chang, National Yang-Ming University, Taiwan
Crystal Structure of Vaccinia Viral A27 Protein Reveals a Novel Structure Critical for its Function and Complex Formation with A26 Protein
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 4 of 8
David C.J. Carpentier, University of Cambridge, UK
Vaccinia Virus F12/E2 Preferentially Interacts with the KLC2 Isoform of Kinesin-1
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 5 of 8
Mathieu Dube, CRCHUM, Canada
Calcium Is Required for Rubella Virus Fusion, Membrane Interaction and Infection
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 6 of 8
Ian B. Hogue, Princeton University, USA
Live-Cell Fluorescence Imaging of Pseudorabies Virus Egress from Infected Cells by Exocytosis
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 7 of 8
Giridhar Murlidharan, University of North Carolina at Chapel Hill, USA
Glycan Binding Affinity Determines Adeno-Associated Viral Spread and Cellular Tropism in the Brain
Thursday, April 03 | 2:30PM - 4:30PM
Workshop 2: Assembly and Exit
Room: Peaks 4-5

Speaker 8 of 8
Coyne G. Drummond, University of Pittsburgh, USA
The Role of Receptor-Interacting Protein Kinase 3 in Coxsackievirus B-Induced Necrosis
Thursday, April 03 | 4:30PM - 5:00PM
Coffee Available
Room: Foyer
Thursday, April 03 | 5:00PM - 7:15PM
Virus Spread II
Room: Peaks 4-5

Speaker 1 of 6
* Michael S. Caffrey, University of Illinois, USA
Thursday, April 03 | 5:00PM - 7:15PM
Virus Spread II
Room: Peaks 4-5

Speaker 2 of 6
Julie K. Pfeiffer, University of Texas Southwestern Medical Center, USA
How Gut Microbes Enhance Enteric Virus Infectivity
Thursday, April 03 | 5:00PM - 7:15PM
Virus Spread II
Room: Peaks 4-5

Speaker 3 of 6
Lynn W. Enquist, Princeton University, USA
Imaging Alpha Herpesvirus Invasion of the Mouse Peripheral Nervous System
Thursday, April 03 | 5:00PM - 7:15PM
Virus Spread II
Room: Peaks 4-5

Speaker 4 of 6
Akira Ono, University of Michigan, USA
HIV-1 Assembly and Spread: Roles Played by Phospholipids and Cell Polarity
Thursday, April 03 | 5:00PM - 7:15PM
Virus Spread II
Room: Peaks 4-5

Speaker 5 of 6
Alison W. Ashbrook, Vanderbilt University, USA
Short Talk: Residue 82 of the Chikungunya Virus E2 Attachment Protein Modulates Viral Dissemination and Arthritis in Mice
Thursday, April 03 | 5:00PM - 7:15PM
Virus Spread II
Room: Peaks 4-5

Speaker 6 of 6
Andrew Mehle, University of Wisconsin, Madison, USA
Short Talk: The Influenza Virus Polymerase Is a Hot Spot for Adaptation during Evolution in Bat Cells
Thursday, April 03 | 7:15PM - 8:15PM
Social Hour with Lite Bites
Room: Peaks 1-3
Thursday, April 03 | 8:00PM - 11:00PM
Entertainment
Room: Peaks 1-3
Thursday, April 03 | 8:00PM - 11:00PM
Cash Bar
Room: Peaks 1-3
Friday, April 04 | 10:25AM - 10:25AM
Departure


*Session Chair.