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Web Desc
Hybrid Methods in Structural Biology
Organizer(s): Jens Meiler, Patrick Cramer and Ron A. Milligan
Date: March 04 - 08, 2015
Location: Granlibakken Tahoe, Tahoe City, CA, USA
Sponsored by Vertex Pharmaceuticals Incorporated
Summary of Meeting:
As we study the structure and dynamics of more and more complex systems to better understand their biological function, each individual technology in the structural biology toolbox approaches its limitations. These limitations include size of the system, timeline of motions, spatial and/or temporal resolution and ensemble averaging. Further, each individual method perturbs the system in a different way to enable or facilitate its analysis. This includes, for example, the introduction of spin labels to perform EPR experiments, the reconstitution of membrane proteins in micelles for NMR spectroscopy, the introduction of entire domains to stabilize proteins in certain conformations for crystallization, or a simplified force field in computation. As systems become more challenging, the perturbations tend to get more drastic, complicating the interpretation of results. Only at the intersection of several techniques does it become possible to distinguish information that is biologically relevant from perturbations introduced by any individual approach. Moving forward, hybrid methods and integrated methods in structural biology play a key role in studying complex systems verifying each other’s results. The objective of this Keystone Symposia meeting is two-fold: 1) To discuss novel and potentially disruptive technologies and/or combinations thereof to be added to the hybrid methods toolbox; and 2) To illustrate and discuss application of hybrid methods to important biological systems. These objectives will be achieved through oral presentations, poster sessions, discussions in panel format and tutorial sessions introducing new software algorithms.
Scholarship Deadline: November 6 2014
Discounted Abstract Deadline: November 6 2014
Abstract Deadline: December 9 2014
Discounted Registration Deadline: January 8 2015
Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:
We gratefully acknowledge additional support for this conference from:
We gratefully acknowledge the generous grant for this conference provided by:
National Institute of General Medical Sciences (NIGMS)
Grant No. 1R13GM113567-01
The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention of trade names, commercial practices, or organizations imply endorsement by the U.S. Government.
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:
Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:
Click here to view more of these organizations
Program
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Wednesday, March 04
| 4:00PM - 8:00PM
Arrival and Registration
Room: Pre Function
Wednesday, March 04
| 6:00PM - 8:00PM
Welcome Mixer
Room: Granhall
No registration fees are used to fund alcohol served at this function.
Wednesday, March 04
| 6:00PM - 8:00PM
Light Meal - Provided by Granlibakken
Room: Granhall
Thursday, March 05
| 7:00AM - 8:00AM
Breakfast
Room: Granhall
Thursday, March 05
| 8:00AM - 9:00AM
Keynote Address
Room: Mountain/Lake
Speaker 1 of 2
* Jens Meiler
, Vanderbilt University, USA
Thursday, March 05
| 8:00AM - 9:00AM
Keynote Address
Room: Mountain/Lake
Speaker 2 of 2
Ad Bax
, NIDDK, National Institutes of Health, USA
When NMR Alone Is Not Enough
Thursday, March 05
| 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons
Room: Mountain/Lake
Speaker 1 of 5
Richard Neutze
, University of Gothenburg, Sweden
Serial Femtosecond Crystallography and Time-Resolved Wide-Angle X-Ray Scattering Studies of a Photosynthetic Reaction Center at an X-Ray Free Electron Laser
Thursday, March 05
| 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons
Room: Mountain/Lake
Speaker 2 of 5
Christian Betzel
, University of Hamburg, Germany
Latest Methods to Grow, Prepare and Score Micro- and Nano-Crystals for Future Free-Electron-Laser and Synchrotron Radiation Sources
Thursday, March 05
| 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons
Room: Mountain/Lake
Speaker 3 of 5
Osamu Miyashita
, RIKEN, Japan
Short Talk: Hybrid Approach for X-Ray Free Electron Laser Single Particle Analysis of Biomolecular Systems
Thursday, March 05
| 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons
Room: Mountain/Lake
Speaker 4 of 5
Basil J. Greber
, University of California, Berkeley, USA
Short Talk: Insights into Structure and Function of the Mammalian Mitochondrial Ribosome
Thursday, March 05
| 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons
Room: Mountain/Lake
Speaker 5 of 5
Stefan Raunser
, Max Planck Institute of Molecular Physiology, Germany
How to Kill a Mocking Bug - Structural Insights into Tc Toxin Complex Action
Thursday, March 05
| 9:40AM - 10:00AM
Coffee Break
Room: Pre Function
Thursday, March 05
| 11:30AM - 1:00PM
Poster Setup
Room: Bay
Thursday, March 05
| 11:30AM - 5:00PM
On Own for Lunch
Thursday, March 05
| 1:00PM - 10:00PM
Poster Viewing
Room: Bay
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 1 of 9
* Andrej Sali
, University of California, San Francisco, USA
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 2 of 9
Charles H. Greenberg
, University of California, San Francisco, USA
Bayesian Modeling of Macromolecular Structures Based on Electron Microscopy Density Maps
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 3 of 9
Abhishek Singharoy
, Arizona State University, USA
Structure Determination with Data-Guided Molecular Dynamics
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 4 of 9
Stephan Niebling
, University of Gothenburg, Sweden
Coarse-Grained Protein X-Ray Scattering Calculations for the Analysis of Time-Resolved X-Ray Scattering
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 5 of 9
Lam T. Nguyen
, California Institute of Technology, USA
Coarse-Grained Simulations Reveal Mechanisms of Bacterial Cell Wall Synthesis
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 6 of 9
Steffen Lindert
, University of California, San Diego, USA
CryoEM-Guided Iterative Molecular Dynamics – Rosetta Protein Structure Refinement Protocol Improves Protein Model Quality
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 7 of 9
Graham T. Johnson
, University of California, San Francisco, USA
Towards Whole Cells Modeled in 3D Molecular Detail and Community Curated with cellPACK
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 8 of 9
Clarence Y. Cheng
, Stanford University, USA
Consistent and Blind Inference of RNA Tertiary Folds from Multidimensional Chemical Mapping Experiments
Thursday, March 05
| 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake
Speaker 9 of 9
Michiel J. van de Waterbeemd
, Utrecht University, Netherlands
Structure and Activity of the RNA Polymerase II - Capping Enzyme Complex Probed by Native Mass Spectrometry and other Structural Biology Methods
Thursday, March 05
| 4:30PM - 5:00PM
Coffee Available
Room: Pre Function
Thursday, March 05
| 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake
Speaker 1 of 4
Andre Hoelz
, California Institute of Technology, USA
Architecture of the Nuclear Pore Complex Coat
Thursday, March 05
| 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake
Speaker 2 of 4
Matthew T. Eddy
, University of Florida, USA
Short Talk: Conformational Dynamics of G Protein-Coupled Receptors Studied by 19F NMR and Novel Isotopic Labeling Approaches
Thursday, March 05
| 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake
Speaker 3 of 4
Leo Sazanov
, Medical Research Council, UK
Structure and Mechanism of Respiratory Complex I
Thursday, March 05
| 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake
Speaker 4 of 4
* Lukas K. Tamm
, University of Virginia, USA
Hybrid Structural Approaches to Resolve the Build-Up of the Exocytotic Fusion Pore
Thursday, March 05
| 7:00PM - 8:00PM
Dinner
Room: Granhall
Thursday, March 05
| 7:30PM - 10:00PM
Poster Session 1
Room: Bay
Thursday, March 05
| 8:00PM - 9:00PM
Social Hour
Room: Bay
No registration fees are used to fund alcohol served at this function.
Friday, March 06
| 7:00AM - 8:00AM
Breakfast
Room: Granhall
Friday, March 06
| 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry
Room: Mountain/Lake
Speaker 1 of 7
* Robert Kaptein
, University of Utrecht, Netherlands
Friday, March 06
| 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry
Room: Mountain/Lake
Speaker 2 of 7
Dorothee Kern
, Brandeis University, HHMI, USA
Ancestor Resurrection as a Microscope into a Cancer Drug’s Mechanism
Friday, March 06
| 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry
Room: Mountain/Lake
Speaker 3 of 7
Mathias Ferber
, Institut Pasteur, France
Short Talk: Integrating the Solvent Accessible Surface Distance with Cross-Links-Based Modeling Methods Improves the Conformational Sampling of Protein Assemblies
Friday, March 06
| 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry
Room: Mountain/Lake
Speaker 4 of 7
Thomas Huber
, Australian National University, Australia
Protein Structure Determination from Sparse Pseudocontact Shifts
Friday, March 06
| 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry
Room: Mountain/Lake
Speaker 5 of 7
Gunnar Jeschke
, ETH Zürich, Switzerland
Structure and Flexibility of Soluble and Membrane Proteins from Sparse Experimental Data
Friday, March 06
| 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry
Room: Mountain/Lake
Speaker 6 of 7
Florian Stengel
, University of Konstanz, Germany
Short Talk: Chemical Cross-Linking/Mass Spectrometry and the Structural Biology Toolbox
Friday, March 06
| 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry
Room: Mountain/Lake
Speaker 7 of 7
Judy E. Kim
, University of California, San Diego, USA
Resonance Raman and FRET Studies of a Beta-Barrel Membrane Protein
Friday, March 06
| 9:20AM - 9:40AM
Coffee Break
Room: Pre Function
Friday, March 06
| 11:00AM - 1:00PM
Poster Setup
Room: Bay
Friday, March 06
| 11:00AM - 5:00PM
On Own for Lunch
Friday, March 06
| 1:00PM - 10:00PM
Poster Viewing
Room: Bay
Friday, March 06
| 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 1 of 5
* Andrej Sali
, University of California, San Francisco, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 2 of 5
Benjamin Webb
, University of California, San Francisco, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 3 of 5
Dina Schneidman
, University of California, San Francisco, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 4 of 5
Charles H. Greenberg
, University of California, San Francisco, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 5 of 5
Seung Joong Kim
, University of California, San Francisco, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 1 of 4
* Jens Meiler
, Vanderbilt University, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 2 of 4
Rocco Moretti
, Vanderbilt University, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 3 of 4
Frank DiMaio
, University of Washington, USA
Friday, March 06
| 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 4 of 4
Nikolaos G. Sgourakis
, University of California, Santa Cruz, USA
Friday, March 06
| 4:30PM - 5:00PM
Coffee Available
Room: Pre Function
Friday, March 06
| 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake
Speaker 1 of 5
* Alasdair C. Steven
, NIAMS, National Institutes of Health, USA
Friday, March 06
| 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake
Speaker 2 of 5
Elizabeth Villa
, University of California, San Diego, USA
Opening Windows into the Cell: Cryo-Electron Tomography of Intact Cells for Structural Cell Biology
Friday, March 06
| 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake
Speaker 3 of 5
Melody G. Campbell
, The Scripps Research Institute, USA
Short Talk: Automated Near Atomic Resolution Electron Microscopy
Friday, March 06
| 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake
Speaker 4 of 5
Frank DiMaio
, University of Washington, USA
Toward Automatic Structure Determination from Near-Atomic-Resolution cryoEM Density Maps
Friday, March 06
| 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake
Speaker 5 of 5
Yifan Cheng
, University of California, San Francisco, USA
Study Membrane Protein Structures by Single Particle cryo-EM
Friday, March 06
| 7:00PM - 8:00PM
Dinner
Room: Granhall
Friday, March 06
| 7:30PM - 10:00PM
Poster Session 2
Room: Bay
Friday, March 06
| 8:00PM - 9:00PM
Social Hour
Room: Bay
No registration fees are used to fund alcohol served at this function.
Saturday, March 07
| 7:00AM - 8:00AM
Breakfast
Room: Granhall
Saturday, March 07
| 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake
Speaker 1 of 7
* Frank DiMaio
, University of Washington, USA
Saturday, March 07
| 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake
Speaker 2 of 7
Patrick Cramer
, Max Planck Institute for Biophysical Chemistry, Germany
Integrated Structural Biology of Genome Transcription
Saturday, March 07
| 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake
Speaker 3 of 7
Andrew B. Ward
, The Scripps Research Institute, USA
Short Talk: Viral Glycoprotein Antibody Complexes
Saturday, March 07
| 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake
Speaker 4 of 7
Andrea Graziadei
, EMBL Heidelberg, Germany
Short Talk: Investigation of the Methylation Mechanism of the Box C/D Ribonucleoprotein Complex
Saturday, March 07
| 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake
Speaker 5 of 7
Alasdair C. Steven
, NIAMS, National Institutes of Health, USA
Mapping Components of Macromolecular Complexes in Two and Three Dimensions by Bubblegram Imaging
Saturday, March 07
| 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake
Speaker 6 of 7
Juli Feigon
, University of California, Los Angeles, USA
The Architecture of Tetrahymena Telomerase Holoenzyme
Saturday, March 07
| 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake
Speaker 7 of 7
Martin Beck
, European Molecular Biology Laboratory, Germany
Integrated Structural Analysis of the Human Nuclear Pore Complex
Saturday, March 07
| 9:20AM - 9:40AM
Coffee Break
Room: Pre Function
Saturday, March 07
| 11:00AM - 5:00PM
On Own for Lunch
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 1 of 9
* Patrick Cramer
, Max Planck Institute for Biophysical Chemistry, Germany
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 2 of 9
Philip Lössl
, Utrecht University, Netherlands
FIRM – An Integrated Mass Spectrometric Approach to Study the Impact of Protein Phosphorylation on the Dynamic Interactions of Mitotic Regulators
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 3 of 9
Vladimir Svetlov
, New York University Langone Medical Center, USA
Short Talk: Docking Model of E. coli RNA Polymerase Complex with Transcription Factor NusA Guided by in vivo Chemical Cross-Links Mapped by Mass Spectrometry
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 4 of 9
Carolina Sánchez-Rico
, Technical University of Munich, Germany
Study of the Conformational Dynamics of the Splicing Factor of U2AF65
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 5 of 9
Sebastian Westenhoff
, University of Gothenburg, Sweden
Signal Transduction in Phytochrome Photosensors Visualized by Time-Resolved
X-Ray Scattering
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 6 of 9
Thomas U. Schwartz
, Massachusetts Institute of Technology, USA
Atomic Structure of the Y-Complex of the Nuclear Pore
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 7 of 9
Audray K. Harris
, NIAID, National Institutes of Health, USA
Characterization of Influenza Virus Subunit Vaccines by Electron Microscopy
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 8 of 9
Alexey Amunts
, Stockholm University, Sweden
Structure of Human Mitochondrial Ribosome Determined by cryo-EM
Saturday, March 07
| 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake
Speaker 9 of 9
Yuehan Feng
, ETH Zürich, Switzerland
Global Analysis of Protein Structural Changes in Complex Proteomes
Saturday, March 07
| 4:30PM - 5:00PM
Coffee Available
Room: Pre Function
Saturday, March 07
| 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake
Speaker 1 of 4
* Michael Nilges
, Institut Pasteur, France
Saturday, March 07
| 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake
Speaker 2 of 4
Sarah Teichmann
, Wellcome Sanger Institute, UK
A Periodic Table of Protein Complexes
Saturday, March 07
| 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake
Speaker 3 of 4
Nikolaos G. Sgourakis
, University of California, Santa Cruz, USA
Modeling Supramolecular Assemblies Using NMR and cryoEM Data
Saturday, March 07
| 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake
Speaker 4 of 4
Jens Meiler
, Vanderbilt University, USA
Protein Structure Determination by Integrating Limited Experimental Data
Saturday, March 07
| 6:45PM - 7:15PM
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Room: Mountain/Lake
Saturday, March 07
| 7:15PM - 8:15PM
Dinner
Room: Granhall
Saturday, March 07
| 7:15PM - 8:15PM
Social Hour
Room: Granhall
No registration fees are used to fund alcohol served at this function.
Saturday, March 07
| 8:00PM - 11:00PM
Entertainment
Room: Granhall
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.
Saturday, March 07
| 8:15PM - 11:00PM
Cash Bar
Room: Granhall
Sunday, March 08
| 10:25AM - 10:25AM
Departure
*Session Chair.
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