Web Desc
Hybrid Methods in Structural Biology
Organizer(s): Jens Meiler, Patrick Cramer and Ron A. Milligan
Date: March 04 - 08, 2015
Location: Granlibakken Tahoe, Tahoe City, CA, USA
Sponsored by Vertex Pharmaceuticals Incorporated
Summary of Meeting:
As we study the structure and dynamics of more and more complex systems to better understand their biological function, each individual technology in the structural biology toolbox approaches its limitations. These limitations include size of the system, timeline of motions, spatial and/or temporal resolution and ensemble averaging. Further, each individual method perturbs the system in a different way to enable or facilitate its analysis. This includes, for example, the introduction of spin labels to perform EPR experiments, the reconstitution of membrane proteins in micelles for NMR spectroscopy, the introduction of entire domains to stabilize proteins in certain conformations for crystallization, or a simplified force field in computation. As systems become more challenging, the perturbations tend to get more drastic, complicating the interpretation of results. Only at the intersection of several techniques does it become possible to distinguish information that is biologically relevant from perturbations introduced by any individual approach. Moving forward, hybrid methods and integrated methods in structural biology play a key role in studying complex systems verifying each other’s results. The objective of this Keystone Symposia meeting is two-fold: 1) To discuss novel and potentially disruptive technologies and/or combinations thereof to be added to the hybrid methods toolbox; and 2) To illustrate and discuss application of hybrid methods to important biological systems. These objectives will be achieved through oral presentations, poster sessions, discussions in panel format and tutorial sessions introducing new software algorithms.
Scholarship Deadline: November 6 2014
Discounted Abstract Deadline: November 6 2014
Abstract Deadline: December 9 2014
Discounted Registration Deadline: January 8 2015
Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:
Vertex Pharmaceuticals Incorporated
We gratefully acknowledge additional support for this conference from:
ISOTEC, a member of the Sigma-Aldrich GroupNew Era Enterprises, Inc.
We gratefully acknowledge the generous grant for this conference provided by:

National Institute of General Medical Sciences (NIGMS)
Grant No. 1R13GM113567-01
The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention of trade names, commercial practices, or organizations imply endorsement by the U.S. Government.
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Wednesday, March 04 | 4:00PM - 8:00PM
Arrival and Registration
Room: Pre Function


Wednesday, March 04 | 6:00PM - 8:00PM
Welcome Mixer
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Wednesday, March 04 | 6:00PM - 8:00PM
Light Meal - Provided by Granlibakken
Room: Granhall


Thursday, March 05 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Thursday, March 05 | 8:00AM - 9:00AM
Keynote Address
Room: Mountain/Lake

Speaker 1 of 2
* Jens Meiler, Vanderbilt University, USA

Thursday, March 05 | 8:00AM - 9:00AM
Keynote Address
Room: Mountain/Lake

Speaker 2 of 2
Ad Bax, NIDDK, National Institutes of Health, USA
When NMR Alone Is Not Enough

Thursday, March 05 | 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons

Room: Mountain/Lake

Speaker 1 of 5
Richard Neutze, University of Gothenburg, Sweden
Serial Femtosecond Crystallography and Time-Resolved Wide-Angle X-Ray Scattering Studies of a Photosynthetic Reaction Center at an X-Ray Free Electron Laser

Thursday, March 05 | 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons

Room: Mountain/Lake

Speaker 2 of 5
Christian Betzel, University of Hamburg, Germany
Latest Methods to Grow, Prepare and Score Micro- and Nano-Crystals for Future Free-Electron-Laser and Synchrotron Radiation Sources

Thursday, March 05 | 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons

Room: Mountain/Lake

Speaker 3 of 5
Osamu Miyashita, RIKEN, Japan
Short Talk: Hybrid Approach for X-Ray Free Electron Laser Single Particle Analysis of Biomolecular Systems

Thursday, March 05 | 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons

Room: Mountain/Lake

Speaker 4 of 5
Basil J. Greber, University of California, Berkeley, USA
Short Talk: Insights into Structure and Function of the Mammalian Mitochondrial Ribosome

Thursday, March 05 | 9:00AM - 11:30AM
New and Potentially Disruptive Technologies: X-Rays, Neutron
s, Electrons

Room: Mountain/Lake

Speaker 5 of 5
Stefan Raunser, Max Planck Institute of Molecular Physiology, Germany
How to Kill a Mocking Bug - Structural Insights into Tc Toxin Complex Action

Thursday, March 05 | 9:40AM - 10:00AM
Coffee Break
Room: Pre Function


Thursday, March 05 | 11:30AM - 1:00PM
Poster Setup
Room: Bay


Thursday, March 05 | 11:30AM - 5:00PM
On Own for Lunch


Thursday, March 05 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 1 of 9
* Andrej Sali, University of California, San Francisco, USA

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 2 of 9
Charles H. Greenberg, University of California, San Francisco, USA
Bayesian Modeling of Macromolecular Structures Based on Electron Microscopy Density Maps

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 3 of 9
Abhishek Singharoy, Arizona State University, USA
Structure Determination with Data-Guided Molecular Dynamics

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 4 of 9
Stephan Niebling, University of Gothenburg, Sweden
Coarse-Grained Protein X-Ray Scattering Calculations for the Analysis of Time-Resolved X-Ray Scattering

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 5 of 9
Lam T. Nguyen, California Institute of Technology, USA
Coarse-Grained Simulations Reveal Mechanisms of Bacterial Cell Wall Synthesis

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 6 of 9
Steffen Lindert, University of California, San Diego, USA
CryoEM-Guided Iterative Molecular Dynamics – Rosetta Protein Structure Refinement Protocol Improves Protein Model Quality

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 7 of 9
Graham T. Johnson, University of California, San Francisco, USA
Towards Whole Cells Modeled in 3D Molecular Detail and Community Curated with cellPACK

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 8 of 9
Clarence Y. Cheng, Stanford University, USA
Consistent and Blind Inference of RNA Tertiary Folds from Multidimensional Chemical Mapping Experiments

Thursday, March 05 | 2:30PM - 4:30PM
Panel Discussion 1
Room: Mountain/Lake

Speaker 9 of 9
Michiel J. van de Waterbeemd, Utrecht University, Netherlands
Structure and Activity of the RNA Polymerase II - Capping Enzyme Complex Probed by Native Mass Spectrometry and other Structural Biology Methods

Thursday, March 05 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Thursday, March 05 | 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake

Speaker 1 of 4
Andre Hoelz, California Institute of Technology, USA
Architecture of the Nuclear Pore Complex Coat

Thursday, March 05 | 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake

Speaker 2 of 4
Matthew T. Eddy, University of Florida, USA
Short Talk: Conformational Dynamics of G Protein-Coupled Receptors Studied by 19F NMR and Novel Isotopic Labeling Approaches

Thursday, March 05 | 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake

Speaker 3 of 4
Leo Sazanov, Medical Research Council, UK
Structure and Mechanism of Respiratory Complex I

Thursday, March 05 | 5:00PM - 7:00PM
Membrane Proteins
Room: Mountain/Lake

Speaker 4 of 4
* Lukas K. Tamm, University of Virginia, USA
Hybrid Structural Approaches to Resolve the Build-Up of the Exocytotic Fusion Pore

Thursday, March 05 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Thursday, March 05 | 7:30PM - 10:00PM
Poster Session 1
Room: Bay


Thursday, March 05 | 8:00PM - 9:00PM
Social Hour
Room: Bay
No registration fees are used to fund alcohol served at this function.

Friday, March 06 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Friday, March 06 | 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry

Room: Mountain/Lake

Speaker 1 of 7
* Robert Kaptein, University of Utrecht, Netherlands

Friday, March 06 | 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry

Room: Mountain/Lake

Speaker 2 of 7
Dorothee Kern, Brandeis University, HHMI, USA
Ancestor Resurrection as a Microscope into a Cancer Drug’s Mechanism

Friday, March 06 | 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry

Room: Mountain/Lake

Speaker 3 of 7
Mathias Ferber, Institut Pasteur, France
Short Talk: Integrating the Solvent Accessible Surface Distance with Cross-Links-Based Modeling Methods Improves the Conformational Sampling of Protein Assemblies

Friday, March 06 | 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry

Room: Mountain/Lake

Speaker 4 of 7
Thomas Huber, Australian National University, Australia
Protein Structure Determination from Sparse Pseudocontact Shifts

Friday, March 06 | 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry

Room: Mountain/Lake

Speaker 5 of 7
Gunnar Jeschke, ETH Zürich, Switzerland
Structure and Flexibility of Soluble and Membrane Proteins from Sparse Experimental Data

Friday, March 06 | 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry

Room: Mountain/Lake

Speaker 6 of 7
Florian Stengel, University of Konstanz, Germany
Short Talk: Chemical Cross-Linking/Mass Spectrometry and the Structural Biology Toolbox

Friday, March 06 | 8:00AM - 11:00AM
New and Potentially Disruptive Technologies: Spectroscopy an
d Spectrometry

Room: Mountain/Lake

Speaker 7 of 7
Judy E. Kim, University of California, San Diego, USA
Resonance Raman and FRET Studies of a Beta-Barrel Membrane Protein

Friday, March 06 | 9:20AM - 9:40AM
Coffee Break
Room: Pre Function


Friday, March 06 | 11:00AM - 1:00PM
Poster Setup
Room: Bay


Friday, March 06 | 11:00AM - 5:00PM
On Own for Lunch


Friday, March 06 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Friday, March 06 | 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 1 of 5
* Andrej Sali, University of California, San Francisco, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 2 of 5
Benjamin Webb, University of California, San Francisco, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 3 of 5
Dina Schneidman, University of California, San Francisco, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 4 of 5
Charles H. Greenberg, University of California, San Francisco, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 1: Integrated Modeling with the Integrative Modelin
g Platform
Room: Granhall
The URL for this workshop is: http://integrativemodeling.org/keystone2015/
Speaker 5 of 5
Seung Joong Kim, University of California, San Francisco, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 1 of 4
* Jens Meiler, Vanderbilt University, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 2 of 4
Rocco Moretti, Vanderbilt University, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 3 of 4
Frank DiMaio, University of Washington, USA

Friday, March 06 | 1:30PM - 4:30PM
Workshop 2: Protein Structure Determination Form Limited Exp
erimental Data with Rosetta
Room: Mountain/Lake
The URL for this workshop is: http://structbio.vanderbilt.edu/comp/workshops/keystone_2015/
Speaker 4 of 4
Nikolaos G. Sgourakis, University of California, Santa Cruz, USA

Friday, March 06 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Friday, March 06 | 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake

Speaker 1 of 5
* Alasdair C. Steven, NIAMS, National Institutes of Health, USA

Friday, March 06 | 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake

Speaker 2 of 5
Elizabeth Villa, University of California, San Diego, USA
Opening Windows into the Cell: Cryo-Electron Tomography of Intact Cells for Structural Cell Biology

Friday, March 06 | 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake

Speaker 3 of 5
Melody G. Campbell, The Scripps Research Institute, USA
Short Talk: Automated Near Atomic Resolution Electron Microscopy

Friday, March 06 | 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake

Speaker 4 of 5
Frank DiMaio, University of Washington, USA
Toward Automatic Structure Determination from Near-Atomic-Resolution cryoEM Density Maps

Friday, March 06 | 5:00PM - 7:00PM
Electron Microscopy
Room: Mountain/Lake

Speaker 5 of 5
Yifan Cheng, University of California, San Francisco, USA
Study Membrane Protein Structures by Single Particle cryo-EM

Friday, March 06 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Friday, March 06 | 7:30PM - 10:00PM
Poster Session 2
Room: Bay


Friday, March 06 | 8:00PM - 9:00PM
Social Hour
Room: Bay
No registration fees are used to fund alcohol served at this function.

Saturday, March 07 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Saturday, March 07 | 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake

Speaker 1 of 7
* Frank DiMaio, University of Washington, USA

Saturday, March 07 | 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake

Speaker 2 of 7
Patrick Cramer, Max Planck Institute for Biophysical Chemistry, Germany
Integrated Structural Biology of Genome Transcription

Saturday, March 07 | 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake

Speaker 3 of 7
Andrew B. Ward, The Scripps Research Institute, USA
Short Talk: Viral Glycoprotein Antibody Complexes

Saturday, March 07 | 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake

Speaker 4 of 7
Andrea Graziadei, EMBL Heidelberg, Germany
Short Talk: Investigation of the Methylation Mechanism of the Box C/D Ribonucleoprotein Complex

Saturday, March 07 | 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake

Speaker 5 of 7
Alasdair C. Steven, NIAMS, National Institutes of Health, USA
Mapping Components of Macromolecular Complexes in Two and Three Dimensions by Bubblegram Imaging

Saturday, March 07 | 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake

Speaker 6 of 7
Juli Feigon, University of California, Los Angeles, USA
The Architecture of Tetrahymena Telomerase Holoenzyme

Saturday, March 07 | 8:00AM - 11:00AM
Supramolecular Assemblies
Room: Mountain/Lake

Speaker 7 of 7
Martin Beck, European Molecular Biology Laboratory, Germany
Integrated Structural Analysis of the Human Nuclear Pore Complex

Saturday, March 07 | 9:20AM - 9:40AM
Coffee Break
Room: Pre Function


Saturday, March 07 | 11:00AM - 5:00PM
On Own for Lunch


Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 1 of 9
* Patrick Cramer, Max Planck Institute for Biophysical Chemistry, Germany

Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 2 of 9
Philip Lössl, Utrecht University, Netherlands
FIRM – An Integrated Mass Spectrometric Approach to Study the Impact of Protein Phosphorylation on the Dynamic Interactions of Mitotic Regulators

Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 3 of 9
Vladimir Svetlov, New York University Langone Medical Center, USA
Short Talk: Docking Model of E. coli RNA Polymerase Complex with Transcription Factor NusA Guided by in vivo Chemical Cross-Links Mapped by Mass Spectrometry

Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 4 of 9
Carolina Sánchez-Rico, Technical University of Munich, Germany
Study of the Conformational Dynamics of the Splicing Factor of U2AF65

Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 5 of 9
Sebastian Westenhoff, University of Gothenburg, Sweden
Signal Transduction in Phytochrome Photosensors Visualized by Time-Resolved
X-Ray Scattering


Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 6 of 9
Thomas U. Schwartz, Massachusetts Institute of Technology, USA
Atomic Structure of the Y-Complex of the Nuclear Pore

Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 7 of 9
Audray K. Harris, NIAID, National Institutes of Health, USA
Characterization of Influenza Virus Subunit Vaccines by Electron Microscopy

Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 8 of 9
Alexey Amunts, Stockholm University, Sweden
Structure of Human Mitochondrial Ribosome Determined by cryo-EM

Saturday, March 07 | 2:30PM - 4:30PM
Panel Discussion 2
Room: Mountain/Lake

Speaker 9 of 9
Yuehan Feng, ETH Zürich, Switzerland
Global Analysis of Protein Structural Changes in Complex Proteomes

Saturday, March 07 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Saturday, March 07 | 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake

Speaker 1 of 4
* Michael Nilges, Institut Pasteur, France

Saturday, March 07 | 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake

Speaker 2 of 4
Sarah Teichmann, Wellcome Sanger Institute, UK
A Periodic Table of Protein Complexes

Saturday, March 07 | 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake

Speaker 3 of 4
Nikolaos G. Sgourakis, University of California, Santa Cruz, USA
Modeling Supramolecular Assemblies Using NMR and cryoEM Data

Saturday, March 07 | 5:00PM - 6:45PM
New and Potentially Disruptive Technologies: Computation
Room: Mountain/Lake

Speaker 4 of 4
Jens Meiler, Vanderbilt University, USA
Protein Structure Determination by Integrating Limited Experimental Data

Saturday, March 07 | 6:45PM - 7:15PM
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Room: Mountain/Lake


Saturday, March 07 | 7:15PM - 8:15PM
Dinner
Room: Granhall


Saturday, March 07 | 7:15PM - 8:15PM
Social Hour
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Saturday, March 07 | 8:00PM - 11:00PM
Entertainment
Room: Granhall
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Saturday, March 07 | 8:15PM - 11:00PM
Cash Bar
Room: Granhall


Sunday, March 08 | 10:25AM - 10:25AM
Departure


*Session Chair.