Web Desc
Small RNA Silencing: Little Guides, Big Biology
Organizer(s): Phillip D. Zamore, Yukihide Tomari and Mihaela Zavolan
Date: January 24 - 28, 2016
Location: Keystone Resort, Keystone, CO, USA
Supported by the Directors' Fund
Summary of Meeting:
Small RNAs have emerged as remarkably pervasive and versatile regulators of gene expression in species ranging from viruses to animals. Although diverse in biogenesis and mode of action, these small, ~21 nucleotide riboregulators, including microRNAs, small interfering RNAs (siRNAs) and PIWI-interacting RNAs, act as guides for Argonaute proteins. As part of these ribonucleoprotein complexes, small RNAs regulate virtually every step of gene expression, playing key roles in nearly every biological process. Nucleic acid-guided Argonautes can be found in eubacteria, archaea, and eukaryotes, suggesting ancient origins of the RNA interference (RNAi) mechanisms. Furthermore, prokaryotes have an RNA-guided immune system — the CRISPR system — that shares functional similarities with RNAi. Although our understanding of these small RNA pathways has progressed rapidly in the past decade, new questions continue to emerge, reflecting our ever more precise and comprehensive methods to quantify the abundance of molecular species and, consequently, the regulatory impact of small RNAs. This meeting seeks to catalyze the open exchange of ideas about the molecular mechanisms by which small RNAs are made and function, the quantitative interplay among small RNAs, between small RNAs and targets, and, ultimately, the consequences of these interactions for cellular function, in normal and pathological states. Special emphasis is placed on showcasing the work of young investigators, and on complementary approaches for studying gene regulation by small RNAs. Finally, the meeting seeks to continue the intellectually rigorous but congenial atmosphere that is the tradition of this exciting field.
Scholarship Deadline: September 28 2015
Discounted Abstract Deadline: September 28 2015
Abstract Deadline: October 28 2015
Discounted Registration Deadline: November 23 2015
We gratefully acknowledge additional support for this conference from:
Dharmacon, part of GE HealthcareTriLink BioTechnologies, Inc.
We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements:

Alnylam Pharmaceuticals, Inc.
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Sunday, January 24 | 4:00PM - 8:00PM
Arrival and Registration
Room: Keystone Lodge Lobby


Sunday, January 24 | 6:00PM - 8:00PM
Welcome Mixer
Room: Keystone Lodge-Bighorn Bar
No registration fees are used to fund alcohol served at this function.

Monday, January 25 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Monday, January 25 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Grays Peak

Speaker 1 of 2
* Phillip D. Zamore, University of Massachusetts Medical School, USA

Monday, January 25 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Grays Peak

Speaker 2 of 2
Julius Brennecke, IMBA - Institut für Molekulare Biotechnologie GmbH, Austria
The piRNA Pathway - A Small RNA Based Genome Defense System

Monday, January 25 | 9:00AM - 11:30AM
Small RNAs in Genome Regulation I
Room: Grays Peak

Speaker 1 of 5
* Celina Juliano, University of California, Davis, USA

Monday, January 25 | 9:00AM - 11:30AM
Small RNAs in Genome Regulation I
Room: Grays Peak

Speaker 2 of 5
Danesh Moazed, Harvard Medical School, USA
Heterochromatic Gene Silencing by small RNAs in S. pombe

Monday, January 25 | 9:00AM - 11:30AM
Small RNAs in Genome Regulation I
Room: Grays Peak

Speaker 3 of 5
Kuniaki Saito, National Institute of Genetics, Japan
Short Talk: Piwi Promotes Transcriptional Silencing by Regulating Chromatin Compaction

Monday, January 25 | 9:00AM - 11:30AM
Small RNAs in Genome Regulation I
Room: Grays Peak

Speaker 4 of 5
Maria Placentino, Institute of Molecular Biology, Germany
Short Talk: Maternal piRNAs are Essential for Germline Development Following de-novo Establishment of Endo-siRNAs in Caenorhabditis elegans

Monday, January 25 | 9:00AM - 11:30AM
Small RNAs in Genome Regulation I
Room: Grays Peak

Speaker 5 of 5
Alexei A. Aravin, California Institute of Technology, USA
Genome Defense by piRNA

Monday, January 25 | 9:40AM - 10:00AM
Coffee Break
Room: Longs Peak Foyer


Monday, January 25 | 11:30AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Monday, January 25 | 11:30AM - 2:30PM
On Own for Lunch


Monday, January 25 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Monday, January 25 | 2:30PM - 4:30PM
Workshop 1: miRNAs
Room: Grays Peak

Speaker 1 of 6
* Martin J. Simard, CRCHU de Québec-Université Laval, Canada

Monday, January 25 | 2:30PM - 4:30PM
Workshop 1: miRNAs
Room: Grays Peak

Speaker 2 of 6
Amy E. Pasquinelli, University of California, San Diego, USA
Distinct Targeting by Let-7 miRNA Family Members

Monday, January 25 | 2:30PM - 4:30PM
Workshop 1: miRNAs
Room: Grays Peak

Speaker 3 of 6
Natalia Pinzon, Centre National de la Recherche Scientifique, France
Titration of miRNAs by mRNAs: A Novel Function for Conserved miRNA Binding Sites

Monday, January 25 | 2:30PM - 4:30PM
Workshop 1: miRNAs
Room: Grays Peak

Speaker 4 of 6
Matteo Osella, Università degli Studi di Torino, Italy
Modelling microRNA Regulation and its Role in Noise Buffering

Monday, January 25 | 2:30PM - 4:30PM
Workshop 1: miRNAs
Room: Grays Peak

Speaker 5 of 6
Francesco Nicassio, Istituto Italiano di Tecnologia, Italy
Insights into Function and Regulation of MicroRNAs by Decoding Degradation Dynamics

Monday, January 25 | 2:30PM - 4:30PM
Workshop 1: miRNAs
Room: Grays Peak

Speaker 6 of 6
Yukihide Tomari, University of Tokyo, Japan
Biochemical and Genetic Approaches to Understanding the MicroRNA Pathway


Monday, January 25 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Monday, January 25 | 5:00PM - 7:00PM
Small RNAs in Genome Regulation II
Room: Grays Peak

Speaker 1 of 5
* Katalin Fejes-Tóth, California Institute of Technology, USA

Monday, January 25 | 5:00PM - 7:00PM
Small RNAs in Genome Regulation II
Room: Grays Peak

Speaker 2 of 5
Michael Terns, University of Georgia, USA
RNA-Guided Invader Defense: CRISPR-Cas Systems

Monday, January 25 | 5:00PM - 7:00PM
Small RNAs in Genome Regulation II
Room: Grays Peak

Speaker 3 of 5
Yijun Qi, Tsinghua University, China
Small RNAs and Genome Stability

Monday, January 25 | 5:00PM - 7:00PM
Small RNAs in Genome Regulation II
Room: Grays Peak

Speaker 4 of 5
Fabrizio d'Adda di Fagagna, IFOM, The FIRC Institute of Molecular Oncology, Italy
Short Talk: RNA: RNA Interactions Control the DNA Damage Response at Individual Genomic Sites

Monday, January 25 | 5:00PM - 7:00PM
Small RNAs in Genome Regulation II
Room: Grays Peak

Speaker 5 of 5
William Theurkauf, University of Massachusetts Medical Center, USA
Gene Conversion Drives Non-Mendelian Inheritance of Transposons and piRNA Clusters in Drosophila melanogaster

Monday, January 25 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Monday, January 25 | 7:30PM - 10:00PM
Poster Session 1
Room: Shavano/Torreys


Tuesday, January 26 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Tuesday, January 26 | 8:00AM - 11:15AM
Diversity of RNA Regulation
Room: Grays Peak

Speaker 1 of 7
* Olivia Rissland, University of Colorado School of Medicine, USA

Tuesday, January 26 | 8:00AM - 11:15AM
Diversity of RNA Regulation
Room: Grays Peak

Speaker 2 of 7
David C. Baulcombe, University of Cambridge, UK
RNA Silencing in Plants

Tuesday, January 26 | 8:00AM - 11:15AM
Diversity of RNA Regulation
Room: Grays Peak

Speaker 3 of 7
Michael J. Axtell, Pennsylvania State University, USA
Small RNA-Producing Genes in Plants: Improved Methods and Novel Discoveries

Tuesday, January 26 | 8:00AM - 11:15AM
Diversity of RNA Regulation
Room: Grays Peak

Speaker 4 of 7
Xuemei Chen, University of California, Riverside, USA
AMP1, ER, and small RNAs

Tuesday, January 26 | 8:00AM - 11:15AM
Diversity of RNA Regulation
Room: Grays Peak

Speaker 5 of 7
Michel J. Tassetto, University of California, San Francisco, USA
Short Talk: Circulating Immune Cells Mediate the Amplification and Memory of a Systemic Nucleic Acid-Based Antiviral Response in Drosophila

Tuesday, January 26 | 8:00AM - 11:15AM
Diversity of RNA Regulation
Room: Grays Peak

Speaker 6 of 7
Valeria Yartseva, Genentech, Inc, USA
Short Talk: The Hidden Code Behind the Genetic Code: Codon Optimality Regulate mRNA Translation and Stability

Tuesday, January 26 | 8:00AM - 11:15AM
Diversity of RNA Regulation
Room: Grays Peak

Speaker 7 of 7
David P. Bartel, Massachusetts Institute of Technology and Whitehead Institute, USA
miRNA-Mediated Gene Regulation

Tuesday, January 26 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Tuesday, January 26 | 11:15AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Tuesday, January 26 | 11:15AM - 2:30PM
On Own for Lunch


Tuesday, January 26 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Tuesday, January 26 | 2:30PM - 4:30PM
Workshop 2: piRNAs
Room: Grays Peak

Speaker 1 of 6
* Julie M. Claycomb, University of Toronto, Canada

Tuesday, January 26 | 2:30PM - 4:30PM
Workshop 2: piRNAs
Room: Grays Peak

Speaker 2 of 6
Mikiko C. Siomi, University of Tokyo, Japan
piRNA Biogenesis in Drosophila

Tuesday, January 26 | 2:30PM - 4:30PM
Workshop 2: piRNAs
Room: Grays Peak

Speaker 3 of 6
Mofang Liu, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
Male Infertility Caused by Ubiquitination-deficient Mutations in Human Piwi (Hiwi)

Tuesday, January 26 | 2:30PM - 4:30PM
Workshop 2: piRNAs
Room: Grays Peak

Speaker 4 of 6
Eric A. Miska, University of Cambridge, UK
Transgenerational Epigenetic Inheritance and RNAe

Tuesday, January 26 | 2:30PM - 4:30PM
Workshop 2: piRNAs
Room: Grays Peak

Speaker 5 of 6
Zsolt Gabor Venkei, University of Michigan, USA
The Role of Kinesin in piRNA Pathway in Drosophila melanogaster Testis

Tuesday, January 26 | 2:30PM - 4:30PM
Workshop 2: piRNAs
Room: Grays Peak

Speaker 6 of 6
Phillip D. Zamore, University of Massachusetts Medical School, USA
piRNAs

Tuesday, January 26 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Tuesday, January 26 | 5:00PM - 7:00PM
Mechanisms of Small RNAs
Room: Grays Peak

Speaker 1 of 5
* Jinbiao Ma, Fudan University, China

Tuesday, January 26 | 5:00PM - 7:00PM
Mechanisms of Small RNAs
Room: Grays Peak

Speaker 2 of 5
V. Narry Kim, Institute for Basic Science, South Korea
Primary MicroRNA Processing by DROSHA

Tuesday, January 26 | 5:00PM - 7:00PM
Mechanisms of Small RNAs
Room: Grays Peak

Speaker 3 of 5
Ian MacRae, The Scripps Research Institute, USA
Structure and Mechanism of Argonaute Proteins

Tuesday, January 26 | 5:00PM - 7:00PM
Mechanisms of Small RNAs
Room: Grays Peak

Speaker 4 of 5
Fuguo Jiang, UT MD Anderson Cancer Center, USA
Short Talk: A Cas9-Guide RNA Complex Pre-Organized for Target DNA Recognition

Tuesday, January 26 | 5:00PM - 7:00PM
Mechanisms of Small RNAs
Room: Grays Peak

Speaker 5 of 5
Kotaro Nakanishi, Ohio State University, USA
Structural and Functional Insights into Mechanisms of Argonaute Proteins

Tuesday, January 26 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Tuesday, January 26 | 7:30PM - 10:00PM
Poster Session 2
Room: Shavano/Torreys


Wednesday, January 27 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 1 of 8
* Nicole Deglon, Lausanne University Hospital, Switzerland

Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 2 of 8
Anastasia Khvorova, University of Massachusetts Medical School, USA
Expanding Chemical Diversity of Therapeutic Oligonucleotides for Treatment of Neurodegenerative Disorders

Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 3 of 8
Si-ping Han, California Institute of Technology, USA
Short Talk: Towards Selective Treatment of Diseased Cells via RNA Transcript Activated Conditional-siRNAs

Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 4 of 8
Muthiah Manoharan, Alnylam Pharmaceuticals, Inc., USA
Therapeutic siRNAs for Human Disease

Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 5 of 8
David M. Rodman, miRagen Therapeutics, USA
Therapeutic Targeting of microRNAs

Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 6 of 8
Fumikazu Shinohara, Kyowa Hakko Kirin Co., Ltd., Japan
Short Talk: Modifications at 5’ End of siRNA Guide Strand Increase siRNA-AGO2 Binding and siRNA Efficacy

Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 7 of 8
Socheata Ly, University of Massachusetts Medical School, USA
Short Talk: Visualization of Self-delivering Hydrophobically Modified siRNA Cellular Internalization

Wednesday, January 27 | 8:00AM - 11:00AM
Small RNAs in Disease and Therapy
Room: Grays Peak

Speaker 8 of 8
Robert A. Martienssen, Cold Spring Harbor Laboratory, USA
Heterochromatin Reprogramming with Small RNA

Wednesday, January 27 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Wednesday, January 27 | 11:00AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Wednesday, January 27 | 11:00AM - 5:00PM
On Own for Lunch


Wednesday, January 27 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Wednesday, January 27 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Wednesday, January 27 | 5:00PM - 7:00PM
MicroRNAs in the Regulation of Cell Fate I
Room: Grays Peak

Speaker 1 of 5
* Olivia Rissland, University of Colorado School of Medicine, USA

Wednesday, January 27 | 5:00PM - 7:00PM
MicroRNAs in the Regulation of Cell Fate I
Room: Grays Peak

Speaker 2 of 5
Donal O'Carroll, University of Edinburgh, UK
Differential Importance of Tut4/7-Mediated Uridylation in vivo

Wednesday, January 27 | 5:00PM - 7:00PM
MicroRNAs in the Regulation of Cell Fate I
Room: Grays Peak

Speaker 3 of 5
Victor R. Ambros, University of Massachusetts, USA
MicroRNAs in Worm Development

Wednesday, January 27 | 5:00PM - 7:00PM
MicroRNAs in the Regulation of Cell Fate I
Room: Grays Peak

Speaker 4 of 5
Chao-Po Lin, University of California, Berkeley, USA
Short Talk: Deficiency of microRNA miR-34a Expands Cell Fate Potential in Pluripotent Stem Cells

Wednesday, January 27 | 5:00PM - 7:00PM
MicroRNAs in the Regulation of Cell Fate I
Room: Grays Peak

Speaker 5 of 5
Richard W. Carthew, Northwestern University, USA
Study of the Mechanism and Function of microRNAs in Drosophila

Wednesday, January 27 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Wednesday, January 27 | 7:30PM - 10:00PM
Poster Session 3
Room: Shavano/Torreys


Thursday, January 28 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Thursday, January 28 | 8:00AM - 11:15AM
MicroRNAs in the Regulation of Cell Fate II
Room: Grays Peak

Speaker 1 of 7
* Alena Shkumatava, Institut Curie-Centre de Recherche, France

Thursday, January 28 | 8:00AM - 11:15AM
MicroRNAs in the Regulation of Cell Fate II
Room: Grays Peak

Speaker 2 of 7
Helge Großhans, Friedrich Miescher Institute - FMI, Switzerland
MicroRNA Function in Vivo: Biology and Regulation

Thursday, January 28 | 8:00AM - 11:15AM
MicroRNAs in the Regulation of Cell Fate II
Room: Grays Peak

Speaker 3 of 7
Carrie B. Wiese, Vanderbilt University, USA
Short Talk: Myeloid-Originating miR-223 Regulates Systemic Glucose and Cholesterol via HDL Intercellular Communication

Thursday, January 28 | 8:00AM - 11:15AM
MicroRNAs in the Regulation of Cell Fate II
Room: Grays Peak

Speaker 4 of 7
Markus Stoffel, ETH Zürich, Switzerland
miR-7 is an Essential Regulator of Endocrine Function

Thursday, January 28 | 8:00AM - 11:15AM
MicroRNAs in the Regulation of Cell Fate II
Room: Grays Peak

Speaker 5 of 7
Anne M. Delany, University of Connecticut Health, USA
Short Talk: MicroRNA Regulation of Circadian Rhythm in the Osteoblastic Lineage

Thursday, January 28 | 8:00AM - 11:15AM
MicroRNAs in the Regulation of Cell Fate II
Room: Grays Peak

Speaker 6 of 7
Stefanie Dimmeler, University of Frankfurt, Germany
miRNAs in the Cardiovascular System

Thursday, January 28 | 8:00AM - 11:15AM
MicroRNAs in the Regulation of Cell Fate II
Room: Grays Peak

Speaker 7 of 7
Nikolaus Rajewsky, Max Delbruck Center for Molecular Medicine, Germany
Regulatory RNAs

Thursday, January 28 | 9:20AM - 9:40AM
Coffee Break
Room: Longs Peak Foyer


Thursday, January 28 | 11:15AM - 2:30PM
On Own for Lunch


Thursday, January 28 | 2:30PM - 4:30PM
Workshop 3: Emerging Technologies and Computational Modeling
Room: Grays Peak

Speaker 1 of 6
* Eric C. Lai, Sloan Kettering Institute, USA

Thursday, January 28 | 2:30PM - 4:30PM
Workshop 3: Emerging Technologies and Computational Modeling
Room: Grays Peak

Speaker 2 of 6
Zhiping Weng, University of Massachusetts, USA
New Computational Methods for Understanding Gene Regulation by Small RNAs

Thursday, January 28 | 2:30PM - 4:30PM
Workshop 3: Emerging Technologies and Computational Modeling
Room: Grays Peak

Speaker 3 of 6
Andrea Riba, University of Basel, Switzerland
Explicit Modeling of siRNA Off-Targets Improves the Recovery of Gene Specific Contribution to Phenotypes

Thursday, January 28 | 2:30PM - 4:30PM
Workshop 3: Emerging Technologies and Computational Modeling
Room: Grays Peak

Speaker 4 of 6
Stefan L. Ameres, Institute of Molecular Biotechnology, Austria
Kinetic Analysis of Small RNA Silencing Pathways in Living Cells

Thursday, January 28 | 2:30PM - 4:30PM
Workshop 3: Emerging Technologies and Computational Modeling
Room: Grays Peak

Speaker 5 of 6
Daniel Cifuentes, Boston University, USA
Uncovering the RNA-Protein Interaction Network that Control Vertebrate Embryogenesis

Thursday, January 28 | 2:30PM - 4:30PM
Workshop 3: Emerging Technologies and Computational Modeling
Room: Grays Peak

Speaker 6 of 6
Mihaela Zavolan, Biozentrum, University of Basel, Switzerland
Exploiting Single Cell Level Heterogeneity to Uncover in Vivo
Hierarchies of miRNA Targets


Thursday, January 28 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Thursday, January 28 | 5:00PM - 6:45PM
Small RNAs in Worms
Room: Grays Peak

Speaker 1 of 4
* René F. Ketting, Institute for Molecular Biology, Germany

Thursday, January 28 | 5:00PM - 6:45PM
Small RNAs in Worms
Room: Grays Peak

Speaker 2 of 4
Andrew Fire, Stanford University, USA
Continuing Education of the Small RNA-ome: Some Mechanisms by which Cells Alter (and Maintain) their Repertoires of Effector RNAs

Thursday, January 28 | 5:00PM - 6:45PM
Small RNAs in Worms
Room: Grays Peak

Speaker 3 of 4
Gary B. Ruvkun, Massachusetts General Hospital, Harvard Medical School, USA
C. elegans Silencing Specializations

Thursday, January 28 | 5:00PM - 6:45PM
Small RNAs in Worms
Room: Grays Peak

Speaker 4 of 4
Craig C. Mello, University of Massachusetts Medical School, USA
RNA-Guided Genome Surveillance

Thursday, January 28 | 6:45PM - 7:00PM
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Room: Grays Peak


Thursday, January 28 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Thursday, January 28 | 8:00PM - 11:00PM
Entertainment
Room: Shavano/Torreys
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Thursday, January 28 | 8:00PM - 11:00PM
Cash Bar
Room: Shavano/Torreys


Friday, January 29 | 10:25AM - 10:25AM
Departure


*Session Chair.