Past Meeting | Keystone Symposia

This meeting took place in the past. Here is a list of meetings that are related:
	Engineering the Genome (2020Q2)
Genome Engineering: From Mechanisms to Therapies (2019B4)

Web Desc
Precision Genome Editing with Programmable Nucleases Organizer(s): Jin-Soo Kim, Feng Zhang and Daniel F. Voytas Date: January 28 - February 01, 2018 Location: Keystone Resort, Keystone, CO, USA Sponsored by Editas Medicine, Regeneron Pharmaceuticals, Inc., Sangamo Therapeutics, Inc. and Vertex Pharmaceuticals Incorporated Summary of Meeting: Genome editing refers to the use of programmable DNA-targeting platforms to manipulate genetic and epigenetic information in living cells and organisms. Genome editing has been recently democratized by the development of RNA-guided endonucleases repurposed from CRISPR-Cas microbial defense systems against viruses or plasmids. Whereas novel tools such as Cpf1 and RNA-guided deaminases are being developed to expand the scope of genome editing, old tools such as ZFNs and TALENs are likely to undergo innovations as well. These tools enable genome-wide genetic screens in cell lines and genetic studies in both model and non-model organisms. Furthermore, genome editing holds great promise for broad applications in medicine and biotechnology, raising public interest and concerns. Yet genome editing is still limited by many factors such as inefficient delivery in vivo, off-target effects, and unwanted, alternative DNA repair. This conference offers opportunities to learn about new developments, advancements and applications in the field. Scholarship Deadline: October 3 2017 Discounted Abstract Deadline: October 3 2017 Abstract Deadline: November 1 2017 Discounted Registration Deadline: December 5 2017 Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting: We gratefully acknowledge additional support from these exhibitors at this conference: We gratefully acknowledge additional support for this conference from: We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements: Editas Medicine We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising: Click here to view more of these organizations Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds: Click here to view more of these organizations

Web Desc

Precision Genome Editing with Programmable Nucleases

Organizer(s): Jin-Soo Kim, Feng Zhang and Daniel F. Voytas

Date: January 28 - February 01, 2018
Location: Keystone Resort, Keystone, CO, USA

Sponsored by Editas Medicine, Regeneron Pharmaceuticals, Inc., Sangamo Therapeutics, Inc. and Vertex Pharmaceuticals Incorporated

Summary of Meeting:

Genome editing refers to the use of programmable DNA-targeting platforms to manipulate genetic and epigenetic information in living cells and organisms. Genome editing has been recently democratized by the development of RNA-guided endonucleases repurposed from CRISPR-Cas microbial defense systems against viruses or plasmids. Whereas novel tools such as Cpf1 and RNA-guided deaminases are being developed to expand the scope of genome editing, old tools such as ZFNs and TALENs are likely to undergo innovations as well. These tools enable genome-wide genetic screens in cell lines and genetic studies in both model and non-model organisms. Furthermore, genome editing holds great promise for broad applications in medicine and biotechnology, raising public interest and concerns. Yet genome editing is still limited by many factors such as inefficient delivery in vivo, off-target effects, and unwanted, alternative DNA repair. This conference offers opportunities to learn about new developments, advancements and applications in the field.

Scholarship Deadline: October 3 2017

Discounted Abstract Deadline: October 3 2017

Abstract Deadline: November 1 2017

Discounted Registration Deadline: December 5 2017

Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:

We gratefully acknowledge additional support from these exhibitors at this conference:

We gratefully acknowledge additional support for this conference from:

We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements:

Editas Medicine

We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations

Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program


Sunday, January 28 \| 4:00PM - 8:00PM Arrival and Registration Room: Longs Peak Foyer
Sunday, January 28 \| 6:00PM - 8:00PM Welcome Mixer Room: Longs Peak Foyer No registration fees are used to fund alcohol served at this function.
Monday, January 29 \| 7:00AM - 8:00AM Breakfast Room: Shavano/Torreys
Monday, January 29 \| 8:00AM - 9:00AM Welcome and Keynote Address Room: Grays/Longs Peak Speaker 1 of 2 * Jin-Soo Kim, Institute for Basic Science, South Korea
Monday, January 29 \| 8:00AM - 9:00AM Welcome and Keynote Address Room: Grays/Longs Peak Speaker 2 of 2 Jennifer A. Doudna, HHMI/University of California, Berkeley, USA CRISPR Systems: Nature’s Toolkit for Genome Editing
Monday, January 29 \| 9:00AM - 11:30AM DNA Repair and Genome Editing Room: Grays/Longs Peak Speaker 1 of 7 * Jacob E. Corn, ETH Zürich, Switzerland
Monday, January 29 \| 9:00AM - 11:30AM DNA Repair and Genome Editing Room: Grays/Longs Peak Speaker 2 of 7 Maria Jasin, Memorial Sloan Kettering Cancer Center, USA Protecting the Genome by Homologous Recombination
Monday, January 29 \| 9:00AM - 11:30AM DNA Repair and Genome Editing Room: Grays/Longs Peak Coffee Break
Monday, January 29 \| 9:00AM - 11:30AM DNA Repair and Genome Editing Room: Grays/Longs Peak Speaker 4 of 7 Eric A. Hendrickson, University of Minnesota Medical School, USA The Mechanisms of Precise Genome Editing Using Oligonucleotide Donors
Monday, January 29 \| 9:00AM - 11:30AM DNA Repair and Genome Editing Room: Grays/Longs Peak Speaker 5 of 7 Eugene V. Koonin, National Institutes of Health, USA CRISPRICITY: A Metric for Measuring CRISPR Association and a Comprehensive Census of Cas Proteins
Monday, January 29 \| 9:00AM - 11:30AM DNA Repair and Genome Editing Room: Grays/Longs Peak Speaker 6 of 7 Chance Meers, Georgia Institute of Technology, USA Short Talk: Transcript-RNA from an Actively Transcribed Gene Accurately Repairs a DNA Double-Strand Break via a Homology- Driven Mechanism
Monday, January 29 \| 9:00AM - 11:30AM DNA Repair and Genome Editing Room: Grays/Longs Peak Speaker 7 of 7 Anastasia Lomova, University of California, Los Angeles, USA Short Talk: Improving Gene Editing by Temporal Control of DNA Repair
Monday, January 29 \| 11:30AM - 11:45AM NIH Somatic Cell Genome Editing Program: Overview Room: Grays/Longs Peak Speaker 1 of 1 * Elizabeth Wilder, National Institutes of Health, USA
Monday, January 29 \| 11:30AM - 5:00PM On Own for Lunch
Monday, January 29 \| 11:30AM - 1:00PM Poster Setup Room: Shavano/Torreys
Monday, January 29 \| 1:00PM - 10:00PM Poster Viewing Room: Shavano/Torreys
Monday, January 29 \| 2:30PM - 4:30PM Workshop: Easi-CRISPR: CRISPRing Made Easier The workshop is based on Easi (Efficient additions with ssDNA inserts)-CRISPR, a recently developed method at the laboratories of Drs. Masato Ohtsuka (Tokai Univeristy, Japan) and C.B. Gurumurthy, which is now becoming a widely used approach for achieving higher knock-in efficiencies via homology directed repair. The workshop will cover: 1) General introduction to animal model generation; 2) Overview of animal model generation methods during pre- and post-CRISPR era; 3) Introduction to Easi-CRISPR and its applications; 4) Examples (case studies) of Easi-CRISPR animal model designs; 5) Hands-on workshop on Easi-CRISPR animal model designs; and 6) Demonstration of animal model designing using participant’s projects (time permitting). Relevant publications on Easi-CRISPR: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1220-4 https://www.nature.com/articles/nprot.2017.153 Pre-prints of Easi-CRISPR: https://www.biorxiv.org/content/early/2017/05/23/141424 https://www.biorxiv.org/content/early/2016/08/17/069963 Speaker 1 of 1 * Gurumurthy B. Channabasavaiah, University of Nebraska Omaha, USA
Monday, January 29 \| 4:30PM - 5:00PM Coffee Available Room: Longs Peak Foyer
Monday, January 29 \| 5:00PM - 7:00PM Genome Editing Methods and Novel Tools I Room: Grays/Longs Peak Speaker 1 of 4 Jonathan Samuel Gootenberg, Harvard University, USA Expanding the Genome Editing Toolbox by Harnessing Microbial Diversity
Monday, January 29 \| 5:00PM - 7:00PM Genome Editing Methods and Novel Tools I Room: Grays/Longs Peak Speaker 2 of 4 David R. Liu, Broad Institute, HHMI, and Harvard University, USA Base Editing: Chemistry on a Target Nucleotide in the Genome of Living Cells
Monday, January 29 \| 5:00PM - 7:00PM Genome Editing Methods and Novel Tools I Room: Grays/Longs Peak Speaker 3 of 4 J. Keith Joung, Massachusetts General Hospital, USA Efficient Gene Editing and Gene Regulation Using CRISPR-Cpf1 Nuclease Technology
Monday, January 29 \| 5:00PM - 7:00PM Genome Editing Methods and Novel Tools I Room: Grays/Longs Peak Speaker 4 of 4 Evan August Boyle, Stanford University, USA Short Talk: High-Throughput Biochemical Profiling of dCas9 Association and Dissociation Kinetics Uncovers New Rules for Off-Target Binding
Monday, January 29 \| 7:00PM - 8:00PM Social Hour with Lite Bites Room: Shavano/Torreys No registration fees are used to fund alcohol served at this function.
Monday, January 29 \| 7:30PM - 10:00PM Poster Session 1 Room: Shavano/Torreys
Tuesday, January 30 \| 7:00AM - 8:00AM Breakfast Room: Shavano/Torreys
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Speaker 1 of 8 * Charles Gersbach, Duke University, USA
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Speaker 2 of 8 Scot A. Wolfe, University of Massachusetts Medical School, USA Orthogonal Cas9-Cas9 Fusions Provide a Versatile Platform for Precise Genome Editing
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Speaker 3 of 8 Jin-Soo Kim, Institute for Basic Science, South Korea Genome-Wide Target Specificities of CRISPR Nucleases and Deaminases
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Coffee Break
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Speaker 5 of 8 Jacob E. Corn, ETH Zürich, Switzerland Discovery of Stimulation-Responsive Immune Enhancers with CRISPR Activation
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Speaker 6 of 8 Keiji Nishida, Kobe University, Japan Genome Editing with Non-Nuclease Editors from Bacteria to Plants
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Speaker 7 of 8 Alister Funnell, Altius Institute for Biomedical Sciences, USA Short Talk: High-Scale in situ Functional Mapping and Quantitative Engineering of Regulatory DNA
Tuesday, January 30 \| 8:00AM - 11:15AM Genome Editing Methods and Novel Tools II Room: Grays/Longs Peak Speaker 8 of 8 Omar Abudayyeh, Massachusetts Institute of Technology, USA Short Talk: Harnessing RNA Targeting CRISPR Systems for Transcriptome Engineering and Human Health
Tuesday, January 30 \| 11:15AM - 5:00PM On Own for Lunch
Tuesday, January 30 \| 11:15AM - 1:00PM Poster Setup Room: Shavano/Torreys
Tuesday, January 30 \| 1:00PM - 10:00PM Poster Viewing Room: Shavano/Torreys
Tuesday, January 30 \| 4:30PM - 5:00PM Coffee Available Room: Longs Peak Foyer
Tuesday, January 30 \| 5:00PM - 7:00PM Epigenome Editing and Gene Regulation Room: Grays/Longs Peak Speaker 1 of 5 * Scot A. Wolfe, University of Massachusetts Medical School, USA
Tuesday, January 30 \| 5:00PM - 7:00PM Epigenome Editing and Gene Regulation Room: Grays/Longs Peak Speaker 2 of 5 Charles Gersbach, Duke University, USA Epigenome Editing for Gene Therapy, Cell Programming and Functional Epigenomics
Tuesday, January 30 \| 5:00PM - 7:00PM Epigenome Editing and Gene Regulation Room: Grays/Longs Peak Speaker 3 of 5 Jennifer Mitchell, University of Toronto, Canada Uncovering Distal Regulatory Element Function Using CRISPR Genome Engineering
Tuesday, January 30 \| 5:00PM - 7:00PM Epigenome Editing and Gene Regulation Room: Grays/Longs Peak Speaker 4 of 5 Joseph M. Miano, University of Rochester, USA Short Talk: CRISPR Interrogation of Regulatory Sequence Function in Mice
Tuesday, January 30 \| 5:00PM - 7:00PM Epigenome Editing and Gene Regulation Room: Grays/Longs Peak Speaker 5 of 5 Chris Richardson, University of California, Berkeley, USA Short Talk: The Fanconi Anemia Pathway Plays a Key Role in Templated Repair at CRISPR-Cas9 Cut Sites
Tuesday, January 30 \| 7:00PM - 8:00PM Social Hour with Lite Bites Room: Shavano/Torreys No registration fees are used to fund alcohol served at this function.
Tuesday, January 30 \| 7:30PM - 10:00PM Poster Session 2 Room: Shavano/Torreys
Wednesday, January 31 \| 7:00AM - 8:00AM Breakfast Room: Shavano/Torreys
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Speaker 1 of 8 * Edward J. Rebar, Sangamo Therapeutics, Inc., USA
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Speaker 2 of 8 Randall J. Platt, ETH Zurich, Switzerland CRISPR Screening Directly in vivo
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Speaker 3 of 8 Reuven Agami, Netherlands Cancer Institute, Netherlands Functional Genetic Screens of Regulatory DNA Elements
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Coffee Break
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Speaker 5 of 8 Neville Sanjana, New York Genome Center & NYU, USA New Frontiers for Pooled Screens: Finding Regulatory Elements in the Noncoding Genome and Capturing Multi-Cell Interactions
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Speaker 6 of 8 Danwei Huangfu, Memorial Sloan Kettering Cancer Institute, USA Human Development and Disease through the Lens of Pluripotent Stem Cells
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Speaker 7 of 8 Bushra Raj, Harvard University, USA Short Talk: Single-Cell Transcriptional Profiling Coupled with Lineage Tracing via Mutable Barcodes in the Vertebrate Brain
Wednesday, January 31 \| 8:00AM - 11:15AM Genetic Screening via CRISPR Room: Grays/Longs Peak Speaker 8 of 8 Jason D. Arroyo, Pfizer, USA Short Talk: Using Human Genetics and Functional Genomics for a Locus-to-Gene Approach to Drug Target Validation
Wednesday, January 31 \| 11:15AM - 12:15PM Panel: Somatic Cell Genome Editing Program: Discussion with NIH Room: Grays/Longs Peak Speaker 1 of 1 * Timothy LaVaute, NINDS, National Institutes of Health, USA
Wednesday, January 31 \| 11:15AM - 5:00PM On Own for Lunch
Wednesday, January 31 \| 11:15AM - 1:00PM Poster Setup Room: Shavano/Torreys
Wednesday, January 31 \| 1:00PM - 10:00PM Poster Viewing Room: Shavano/Torreys
Wednesday, January 31 \| 4:30PM - 5:00PM Coffee Available Room: Longs Peak Foyer
Wednesday, January 31 \| 5:00PM - 7:00PM Therapeutic Genome Editing Room: Grays/Longs Peak Speaker 1 of 5 * Randall J. Platt, ETH Zurich, Switzerland
Wednesday, January 31 \| 5:00PM - 7:00PM Therapeutic Genome Editing Room: Grays/Longs Peak Speaker 2 of 5 Edward J. Rebar, Sangamo Therapeutics, Inc., USA Therapeutic Genome Editing and Gene Regulation Using Designed Zinc Finger Proteins
Wednesday, January 31 \| 5:00PM - 7:00PM Therapeutic Genome Editing Room: Grays/Longs Peak Speaker 3 of 5 Amy J. Wagers, Harvard University, USA In vivo Gene Editing in Muscle and Muscle Stem Cells
Wednesday, January 31 \| 5:00PM - 7:00PM Therapeutic Genome Editing Room: Grays/Longs Peak Speaker 4 of 5 Charles F. Albright, Editas Medicine, USA Development of a Subretinally-Delivered, CEP290-Specific CRISPR Medicine for the Treatment of Leber Congenital Amaurosis 10 (LCA10)
Wednesday, January 31 \| 5:00PM - 7:00PM Therapeutic Genome Editing Room: Grays/Longs Peak Speaker 5 of 5 Pavitra Roychoudhury, University of Washington, USA Short Talk: Rational Design and Evaluation of CRISPR/Cas9 Strategies for HIV Cure
Wednesday, January 31 \| 7:00PM - 8:00PM Social Hour with Lite Bites Room: Shavano/Torreys No registration fees are used to fund alcohol served at this function.
Wednesday, January 31 \| 7:30PM - 10:00PM Poster Session 3 Room: Shavano/Torreys
Thursday, February 01 \| 7:00AM - 8:00AM Breakfast Room: Shavano/Torreys
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Speaker 1 of 8 * Daniel F. Voytas, University of Minnesota, USA
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Speaker 2 of 8 Dan Carlson, Recombinetics, Inc., USA Large Animal Genome Editing for Agriculture and Biomedicine
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Speaker 3 of 8 Caixia Gao, Chinese Academy of Sciences, China Genome Editing with Programmable Nucleases in Crop Plants
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Coffee Break
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Speaker 5 of 8 Daniel F. Voytas, University of Minnesota, USA Editing the Plant Genome
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Speaker 6 of 8 Weizhi Ji, Kunming University of Science and Technology, China Primate Gene Editing and Human Complex Disease Study
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Speaker 7 of 8 Mariette E.S. Andersson, Swedish University of Agricultural Sciences, Sweden Short Talk: CRISPR-Cas9 Takes Several Bites in the Potato Genome- Efficient Targeted Multiallelic Mutagenesis in Tetraploid Solanum tuberosum
Thursday, February 01 \| 8:00AM - 11:15AM Plant and Animal Biotechnology Room: Grays/Longs Peak Speaker 8 of 8 Asim Ahmad Beg, University of Michigan, USA Short Talk: A Rapid and Facile C. elegans Genome Engineering Pipeline Using CRISPR/Cas9 Ribonucleoproteins
Thursday, February 01 \| 11:15AM - 5:00PM On Own for Lunch
Thursday, February 01 \| 4:30PM - 5:00PM Coffee Available Room: Longs Peak Foyer
Thursday, February 01 \| 5:00PM - 6:45PM Human Germline Editing and ELSI Room: Grays/Longs Peak Speaker 1 of 4 * Jin-Soo Kim, Institute for Basic Science, South Korea
Thursday, February 01 \| 5:00PM - 6:45PM Human Germline Editing and ELSI Room: Grays/Longs Peak Speaker 2 of 4 Kathy K. Niakan, Francis Crick Institute, UK Genome Editing Reveals a Role for OCT4 in Human Embryogenesis
Thursday, February 01 \| 5:00PM - 6:45PM Human Germline Editing and ELSI Room: Grays/Longs Peak Speaker 3 of 4 Shoukrat Mitalipov, Oregon Health & Science University, USA Human Germline Gene Correction
Thursday, February 01 \| 5:00PM - 6:45PM Human Germline Editing and ELSI Room: Grays/Longs Peak Speaker 4 of 4 Tetsuya Ishii, Hokkaido University, Japan The Potential Guidelines for Germline Genome Editing in Clinics
Thursday, February 01 \| 6:45PM - 7:00PM Meeting Wrap-Up: Outcomes and Future Directions (Organizers) Room: Grays/Longs Peak
Thursday, February 01 \| 7:00PM - 8:00PM Social Hour with Lite Bites Room: Shavano/Torreys No registration fees are used to fund alcohol served at this function.
Thursday, February 01 \| 8:00PM - 11:00PM Entertainment Room: Shavano/Torreys Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.
Thursday, February 01 \| 8:00PM - 11:00PM Cash Bar Room: Shavano/Torreys
Friday, February 02 \| 10:24AM - 10:24AM Departure *Session Chair.