Web Desc
Precision Genome Editing with Programmable Nucleases
Organizer(s): Jin-Soo Kim, Feng Zhang and Daniel F. Voytas
Date: January 28 - February 01, 2018
Location: Keystone Resort, Keystone, CO, USA
Sponsored by Editas Medicine, Regeneron Pharmaceuticals, Inc., Sangamo Therapeutics, Inc. and Vertex Pharmaceuticals Incorporated
Summary of Meeting:
Genome editing refers to the use of programmable DNA-targeting platforms to manipulate genetic and epigenetic information in living cells and organisms. Genome editing has been recently democratized by the development of RNA-guided endonucleases repurposed from CRISPR-Cas microbial defense systems against viruses or plasmids. Whereas novel tools such as Cpf1 and RNA-guided deaminases are being developed to expand the scope of genome editing, old tools such as ZFNs and TALENs are likely to undergo innovations as well. These tools enable genome-wide genetic screens in cell lines and genetic studies in both model and non-model organisms. Furthermore, genome editing holds great promise for broad applications in medicine and biotechnology, raising public interest and concerns. Yet genome editing is still limited by many factors such as inefficient delivery in vivo, off-target effects, and unwanted, alternative DNA repair. This conference offers opportunities to learn about new developments, advancements and applications in the field.
Scholarship Deadline: October 3 2017
Discounted Abstract Deadline: October 3 2017
Abstract Deadline: November 1 2017
Discounted Registration Deadline: December 5 2017
Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:
Editas MedicineRegeneron Pharmaceuticals, Inc.Sangamo Therapeutics, Inc.Vertex Pharmaceuticals Incorporated
We gratefully acknowledge additional support from these exhibitors at this conference:
Agilent Technologies, Inc.Dharmacon, a Horizon Discovery Group CompanySynthego
We gratefully acknowledge additional support for this conference from:
American Society of Gene & Cell Therapy (ASGCT)Integrated DNA Technologies
We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements:

Editas Medicine
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Sunday, January 28 | 4:00PM - 8:00PM
Arrival and Registration
Room: Longs Peak Foyer


Sunday, January 28 | 6:00PM - 8:00PM
Welcome Mixer
Room: Longs Peak Foyer
No registration fees are used to fund alcohol served at this function.

Monday, January 29 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Monday, January 29 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Grays/Longs Peak

Speaker 1 of 2
* Jin-Soo Kim, Institute for Basic Science, South Korea

Monday, January 29 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Grays/Longs Peak

Speaker 2 of 2
Jennifer A. Doudna, HHMI/University of California, Berkeley, USA
CRISPR Systems: Nature’s Toolkit for Genome Editing

Monday, January 29 | 9:00AM - 11:30AM
DNA Repair and Genome Editing
Room: Grays/Longs Peak

Speaker 1 of 7
* Jacob E. Corn, ETH Zürich, Switzerland

Monday, January 29 | 9:00AM - 11:30AM
DNA Repair and Genome Editing
Room: Grays/Longs Peak

Speaker 2 of 7
Maria Jasin, Memorial Sloan Kettering Cancer Center, USA
Protecting the Genome by Homologous Recombination

Monday, January 29 | 9:00AM - 11:30AM
DNA Repair and Genome Editing
Room: Grays/Longs Peak


Coffee Break

Monday, January 29 | 9:00AM - 11:30AM
DNA Repair and Genome Editing
Room: Grays/Longs Peak

Speaker 4 of 7
Eric A. Hendrickson, University of Minnesota Medical School, USA
The Mechanisms of Precise Genome Editing Using Oligonucleotide Donors

Monday, January 29 | 9:00AM - 11:30AM
DNA Repair and Genome Editing
Room: Grays/Longs Peak

Speaker 5 of 7
Eugene V. Koonin, National Institutes of Health, USA
CRISPRICITY: A Metric for Measuring CRISPR Association and a Comprehensive Census of Cas Proteins

Monday, January 29 | 9:00AM - 11:30AM
DNA Repair and Genome Editing
Room: Grays/Longs Peak

Speaker 6 of 7
Chance Meers, Georgia Institute of Technology, USA
Short Talk: Transcript-RNA from an Actively Transcribed Gene Accurately Repairs a DNA Double-Strand Break via a Homology-
Driven Mechanism


Monday, January 29 | 9:00AM - 11:30AM
DNA Repair and Genome Editing
Room: Grays/Longs Peak

Speaker 7 of 7
Anastasia Lomova, University of California, Los Angeles, USA
Short Talk: Improving Gene Editing by Temporal Control of DNA Repair

Monday, January 29 | 11:30AM - 11:45AM
NIH Somatic Cell Genome Editing Program: Overview
Room: Grays/Longs Peak

Speaker 1 of 1
* Elizabeth Wilder, National Institutes of Health, USA

Monday, January 29 | 11:30AM - 5:00PM
On Own for Lunch


Monday, January 29 | 11:30AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Monday, January 29 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Monday, January 29 | 2:30PM - 4:30PM
Workshop: Easi-CRISPR: CRISPRing Made Easier
The workshop is based on Easi (Efficient additions with ssDNA inserts)-CRISPR, a recently developed method at the laboratories of Drs. Masato Ohtsuka (Tokai Univeristy, Japan) and C.B. Gurumurthy, which is now becoming a widely used approach for achieving higher knock-in efficiencies via homology directed repair. The workshop will cover: 1) General introduction to animal model generation; 2) Overview of animal model generation methods during pre- and post-CRISPR era; 3) Introduction to Easi-CRISPR and its applications; 4) Examples (case studies) of Easi-CRISPR animal model designs; 5) Hands-on workshop on Easi-CRISPR animal model designs; and 6) Demonstration of animal model designing using participant’s projects (time permitting).


Relevant publications on Easi-CRISPR:
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1220-4
https://www.nature.com/articles/nprot.2017.153


Pre-prints of Easi-CRISPR:
https://www.biorxiv.org/content/early/2017/05/23/141424
https://www.biorxiv.org/content/early/2016/08/17/069963
Speaker 1 of 1
* Gurumurthy B. Channabasavaiah, University of Nebraska Omaha, USA

Monday, January 29 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Monday, January 29 | 5:00PM - 7:00PM
Genome Editing Methods and Novel Tools I
Room: Grays/Longs Peak

Speaker 1 of 4
Jonathan Samuel Gootenberg, Harvard University, USA
Expanding the Genome Editing Toolbox by Harnessing Microbial Diversity

Monday, January 29 | 5:00PM - 7:00PM
Genome Editing Methods and Novel Tools I
Room: Grays/Longs Peak

Speaker 2 of 4
David R. Liu, Broad Institute, HHMI, and Harvard University, USA
Base Editing: Chemistry on a Target Nucleotide in the Genome of Living Cells

Monday, January 29 | 5:00PM - 7:00PM
Genome Editing Methods and Novel Tools I
Room: Grays/Longs Peak

Speaker 3 of 4
J. Keith Joung, Massachusetts General Hospital, USA
Efficient Gene Editing and Gene Regulation Using CRISPR-Cpf1 Nuclease Technology

Monday, January 29 | 5:00PM - 7:00PM
Genome Editing Methods and Novel Tools I
Room: Grays/Longs Peak

Speaker 4 of 4
Evan August Boyle, Stanford University, USA
Short Talk: High-Throughput Biochemical Profiling of dCas9 Association and Dissociation Kinetics Uncovers New Rules for Off-Target Binding

Monday, January 29 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Monday, January 29 | 7:30PM - 10:00PM
Poster Session 1
Room: Shavano/Torreys


Tuesday, January 30 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak

Speaker 1 of 8
* Charles Gersbach, Duke University, USA

Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak

Speaker 2 of 8
Scot A. Wolfe, University of Massachusetts Medical School, USA
Orthogonal Cas9-Cas9 Fusions Provide a Versatile Platform for Precise Genome Editing

Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak

Speaker 3 of 8
Jin-Soo Kim, Institute for Basic Science, South Korea
Genome-Wide Target Specificities of CRISPR Nucleases and Deaminases

Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak


Coffee Break

Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak

Speaker 5 of 8
Jacob E. Corn, ETH Zürich, Switzerland
Discovery of Stimulation-Responsive Immune Enhancers with CRISPR Activation

Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak

Speaker 6 of 8
Keiji Nishida, Kobe University, Japan
Genome Editing with Non-Nuclease Editors from Bacteria to Plants

Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak

Speaker 7 of 8
Alister Funnell, Altius Institute for Biomedical Sciences, USA
Short Talk: High-Scale in situ Functional Mapping and Quantitative Engineering of Regulatory DNA

Tuesday, January 30 | 8:00AM - 11:15AM
Genome Editing Methods and Novel Tools II
Room: Grays/Longs Peak

Speaker 8 of 8
Omar Abudayyeh, Massachusetts Institute of Technology, USA
Short Talk: Harnessing RNA Targeting CRISPR Systems for Transcriptome Engineering and Human Health

Tuesday, January 30 | 11:15AM - 5:00PM
On Own for Lunch


Tuesday, January 30 | 11:15AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Tuesday, January 30 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Tuesday, January 30 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Tuesday, January 30 | 5:00PM - 7:00PM
Epigenome Editing and Gene Regulation
Room: Grays/Longs Peak

Speaker 1 of 5
* Scot A. Wolfe, University of Massachusetts Medical School, USA

Tuesday, January 30 | 5:00PM - 7:00PM
Epigenome Editing and Gene Regulation
Room: Grays/Longs Peak

Speaker 2 of 5
Charles Gersbach, Duke University, USA
Epigenome Editing for Gene Therapy, Cell Programming and Functional Epigenomics

Tuesday, January 30 | 5:00PM - 7:00PM
Epigenome Editing and Gene Regulation
Room: Grays/Longs Peak

Speaker 3 of 5
Jennifer Mitchell, University of Toronto, Canada
Uncovering Distal Regulatory Element Function Using CRISPR Genome Engineering

Tuesday, January 30 | 5:00PM - 7:00PM
Epigenome Editing and Gene Regulation
Room: Grays/Longs Peak

Speaker 4 of 5
Joseph M. Miano, University of Rochester, USA
Short Talk: CRISPR Interrogation of Regulatory Sequence Function in Mice

Tuesday, January 30 | 5:00PM - 7:00PM
Epigenome Editing and Gene Regulation
Room: Grays/Longs Peak

Speaker 5 of 5
Chris Richardson, University of California, Berkeley, USA
Short Talk: The Fanconi Anemia Pathway Plays a Key Role in Templated Repair at CRISPR-Cas9 Cut Sites

Tuesday, January 30 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Tuesday, January 30 | 7:30PM - 10:00PM
Poster Session 2
Room: Shavano/Torreys


Wednesday, January 31 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak

Speaker 1 of 8
* Edward J. Rebar, Sangamo Therapeutics, Inc., USA

Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak

Speaker 2 of 8
Randall J. Platt, ETH Zurich, Switzerland
CRISPR Screening Directly in vivo

Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak

Speaker 3 of 8
Reuven Agami, Netherlands Cancer Institute, Netherlands
Functional Genetic Screens of Regulatory DNA Elements

Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak


Coffee Break

Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak

Speaker 5 of 8
Neville Sanjana, New York Genome Center & NYU, USA
New Frontiers for Pooled Screens: Finding Regulatory Elements in the Noncoding Genome and Capturing Multi-Cell Interactions

Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak

Speaker 6 of 8
Danwei Huangfu, Memorial Sloan Kettering Cancer Institute, USA
Human Development and Disease through the Lens of Pluripotent Stem Cells

Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak

Speaker 7 of 8
Bushra Raj, Harvard University, USA
Short Talk: Single-Cell Transcriptional Profiling Coupled with Lineage Tracing via Mutable Barcodes in the Vertebrate Brain

Wednesday, January 31 | 8:00AM - 11:15AM
Genetic Screening via CRISPR
Room: Grays/Longs Peak

Speaker 8 of 8
Jason D. Arroyo, Pfizer, USA
Short Talk: Using Human Genetics and Functional Genomics for a Locus-to-Gene Approach to Drug Target Validation

Wednesday, January 31 | 11:15AM - 12:15PM
Panel: Somatic Cell Genome Editing Program: Discussion with
NIH
Room: Grays/Longs Peak

Speaker 1 of 1
* Timothy LaVaute, NINDS, National Institutes of Health, USA

Wednesday, January 31 | 11:15AM - 5:00PM
On Own for Lunch


Wednesday, January 31 | 11:15AM - 1:00PM
Poster Setup
Room: Shavano/Torreys


Wednesday, January 31 | 1:00PM - 10:00PM
Poster Viewing
Room: Shavano/Torreys


Wednesday, January 31 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Wednesday, January 31 | 5:00PM - 7:00PM
Therapeutic Genome Editing
Room: Grays/Longs Peak

Speaker 1 of 5
* Randall J. Platt, ETH Zurich, Switzerland

Wednesday, January 31 | 5:00PM - 7:00PM
Therapeutic Genome Editing
Room: Grays/Longs Peak

Speaker 2 of 5
Edward J. Rebar, Sangamo Therapeutics, Inc., USA
Therapeutic Genome Editing and Gene Regulation Using Designed Zinc Finger Proteins

Wednesday, January 31 | 5:00PM - 7:00PM
Therapeutic Genome Editing
Room: Grays/Longs Peak

Speaker 3 of 5
Amy J. Wagers, Harvard University, USA
In vivo Gene Editing in Muscle and Muscle Stem Cells

Wednesday, January 31 | 5:00PM - 7:00PM
Therapeutic Genome Editing
Room: Grays/Longs Peak

Speaker 4 of 5
Charles F. Albright, Editas Medicine, USA
Development of a Subretinally-Delivered, CEP290-Specific CRISPR Medicine for the Treatment of Leber Congenital Amaurosis 10 (LCA10)

Wednesday, January 31 | 5:00PM - 7:00PM
Therapeutic Genome Editing
Room: Grays/Longs Peak

Speaker 5 of 5
Pavitra Roychoudhury, University of Washington, USA
Short Talk: Rational Design and Evaluation of CRISPR/Cas9 Strategies for HIV Cure

Wednesday, January 31 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Wednesday, January 31 | 7:30PM - 10:00PM
Poster Session 3
Room: Shavano/Torreys


Thursday, February 01 | 7:00AM - 8:00AM
Breakfast
Room: Shavano/Torreys


Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak

Speaker 1 of 8
* Daniel F. Voytas, University of Minnesota, USA

Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak

Speaker 2 of 8
Dan Carlson, Recombinetics, Inc., USA
Large Animal Genome Editing for Agriculture and Biomedicine

Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak

Speaker 3 of 8
Caixia Gao, Chinese Academy of Sciences, China
Genome Editing with Programmable Nucleases in Crop Plants

Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak


Coffee Break

Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak

Speaker 5 of 8
Daniel F. Voytas, University of Minnesota, USA
Editing the Plant Genome

Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak

Speaker 6 of 8
Weizhi Ji, Kunming University of Science and Technology, China
Primate Gene Editing and Human Complex Disease Study

Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak

Speaker 7 of 8
Mariette E.S. Andersson, Swedish University of Agricultural Sciences, Sweden
Short Talk: CRISPR-Cas9 Takes Several Bites in the Potato Genome- Efficient Targeted Multiallelic Mutagenesis in Tetraploid Solanum tuberosum

Thursday, February 01 | 8:00AM - 11:15AM
Plant and Animal Biotechnology
Room: Grays/Longs Peak

Speaker 8 of 8
Asim Ahmad Beg, University of Michigan, USA
Short Talk: A Rapid and Facile C. elegans Genome Engineering Pipeline Using CRISPR/Cas9 Ribonucleoproteins

Thursday, February 01 | 11:15AM - 5:00PM
On Own for Lunch


Thursday, February 01 | 4:30PM - 5:00PM
Coffee Available
Room: Longs Peak Foyer


Thursday, February 01 | 5:00PM - 6:45PM
Human Germline Editing and ELSI
Room: Grays/Longs Peak

Speaker 1 of 4
* Jin-Soo Kim, Institute for Basic Science, South Korea

Thursday, February 01 | 5:00PM - 6:45PM
Human Germline Editing and ELSI
Room: Grays/Longs Peak

Speaker 2 of 4
Kathy K. Niakan, Francis Crick Institute, UK
Genome Editing Reveals a Role for OCT4 in Human Embryogenesis

Thursday, February 01 | 5:00PM - 6:45PM
Human Germline Editing and ELSI
Room: Grays/Longs Peak

Speaker 3 of 4
Shoukrat Mitalipov, Oregon Health & Science University, USA
Human Germline Gene Correction

Thursday, February 01 | 5:00PM - 6:45PM
Human Germline Editing and ELSI
Room: Grays/Longs Peak

Speaker 4 of 4
Tetsuya Ishii, Hokkaido University, Japan
The Potential Guidelines for Germline Genome Editing in Clinics

Thursday, February 01 | 6:45PM - 7:00PM
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Room: Grays/Longs Peak


Thursday, February 01 | 7:00PM - 8:00PM
Social Hour with Lite Bites
Room: Shavano/Torreys
No registration fees are used to fund alcohol served at this function.

Thursday, February 01 | 8:00PM - 11:00PM
Entertainment
Room: Shavano/Torreys
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Thursday, February 01 | 8:00PM - 11:00PM
Cash Bar
Room: Shavano/Torreys


Friday, February 02 | 10:24AM - 10:24AM
Departure


*Session Chair.