Web Desc
Cryo-EM from Cells to Molecules: Multi-Scale Visualization of Biological Systems
Organizer(s): Georgios Skiniotis, Elizabeth Villa and Andrew B. Ward
Date: February 04 - 08, 2018
Location: Granlibakken Tahoe, Tahoe City, CA, USA
Sponsored by Pfizer Inc.
For important information on the coronavirus, please click here
Summary of Meeting:
Fast-evolving cryo-EM visualization techniques have recently started to illuminate the inner workings of complicated and dynamic biological assemblies with a level of detail we have never seen before. Cryo-EM is now the state-of-the-art methodology for taking snapshots of biological systems from low to high resolution and with various levels of complexities, from small macromolecular complexes all the way to subcellular organizations. This symposium will showcase cryo-EM breakthroughs in addressing crucial problems in cell biology and will further highlight cryo-EM imaging as a platform for the integration of data from orthogonal approaches. With talks on a variety of biological specimens, procedures and types of biological questions, the meeting aims to attract a very diverse audience from both academia and industry with interest in the method, and beyond the traditional cryo-EM specialist. The symposium will focus on four themes: 1) Single-particle cryo-EM with attention to the characterization of the conformational ensemble adopted by macromolecular complexes and aiming to delineate their mechanism of action; 2) Cryo-electron tomography (cryo-ET) and correlative light electron microscopy (CLEM) to visualize cellular organizations and their dynamics; 3) The emergence of cryo-EM as tool for drug discovery and translational research through both high-resolution structure determination and characterization of cellular phenotypes; and 4) Recent developments in sample preparation and image acquisition. Furthermore, the symposium will include workshops in specimen preparation and evaluation of suitability for cryo-EM, modeling of structures using cryo-EM data, and how to set up a cryo-EM lab.
Scholarship Deadline: October 5 2017
Discounted Abstract Deadline: October 5 2017
Abstract Deadline: November 7 2017
Discounted Registration Deadline: December 7 2017
Abstract submission is now closed. Registered attendees may bring a poster onsite. Please contact our office at +1 800-253-0685; +1 970-262-1230 or email info@keystonesymposia.org if you are interested.


Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:
Pfizer Inc.
We gratefully acknowledge additional support from these exhibitors at this conference:
Thermo Fisher Scientific
We gratefully acknowledge additional support for this conference from:
Direct Electron, LP
MiTeGen
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Sunday, February 04 | 4:00PM - 8:00PM
Arrival and Registration
Room: Pre Function


Sunday, February 04 | 6:00PM - 8:00PM
Welcome Mixer
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Sunday, February 04 | 6:00PM - 8:00PM
Light Meal - Provided by Granlibakken
Room: Granhall


Monday, February 05 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Monday, February 05 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 1 of 4
* Andrew B. Ward, The Scripps Research Institute, USA

Monday, February 05 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 2 of 4
* Elizabeth Villa, University of California, San Diego, USA

Monday, February 05 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 3 of 4
* Georgios Skiniotis, Stanford University, USA

Monday, February 05 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 4 of 4
Patrick Cramer, Max Planck Institute for Biophysical Chemistry, Germany
Integrated Structural Biology of Gene Transcription

Monday, February 05 | 9:00AM - 11:30AM
Visualizing Cells
Room: Mountain/Lake

Speaker 1 of 6
* Elizabeth Villa, University of California, San Diego, USA
Opening Windows into the Cell: Bringing Structure to Cell Biology using Cryo-Electron Tomography

Monday, February 05 | 9:00AM - 11:30AM
Visualizing Cells
Room: Mountain/Lake


Coffee Break

Monday, February 05 | 9:00AM - 11:30AM
Visualizing Cells
Room: Mountain/Lake

Speaker 3 of 6
Grant J. Jensen, California Institute of Technology, USA
Structure and Function of the Bacterial Type IV Secretion System through Electron Cryotomography

Monday, February 05 | 9:00AM - 11:30AM
Visualizing Cells
Room: Mountain/Lake

Speaker 4 of 6
Mark H. Ellisman, University of California, San Diego, USA
Revealing Secrets Hiding in Plain Sight

Monday, February 05 | 9:00AM - 11:30AM
Visualizing Cells
Room: Mountain/Lake

Speaker 5 of 6
Christina Heroven, MRC Laboratory of Molecular Biology, UK
Short Talk: In-situ Structural Studies of RPTPmu by Cryo-Electron Tomography of FIB Milled Cells

Monday, February 05 | 9:00AM - 11:30AM
Visualizing Cells
Room: Mountain/Lake

Speaker 6 of 6
Kanika Khanna, University of California, San Diego, USA
Short Talk: Understanding Morphological Changes Accompanying Sporulation in B. subtilis using in situ Cryo-Electron Tomography

Monday, February 05 | 11:30AM - 5:00PM
On Own for Lunch


Monday, February 05 | 11:30AM - 1:00PM
Poster Setup
Room: Bay


Monday, February 05 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Monday, February 05 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Monday, February 05 | 5:00PM - 7:00PM
Visualizing Subcellular Compartments
Room: Mountain/Lake

Speaker 1 of 4
Daniela Nicastro, University of Texas Southwestern Medical Center, USA
Probing the Molecular Organization of Cells and Organelles using Cryo-Electron Microscopy

Monday, February 05 | 5:00PM - 7:00PM
Visualizing Subcellular Compartments
Room: Mountain/Lake

Speaker 2 of 4
Jun Liu, Yale School of Medicine, USA
High-Throughput Cryo-Electron Tomography: Visualizing Host-Pathogen Interactions at High Resolution

Monday, February 05 | 5:00PM - 7:00PM
Visualizing Subcellular Compartments
Room: Mountain/Lake

Speaker 3 of 4
* Kay Grünewald, University of Oxford, UK
Trafficking Vesicles and Enveloped Viruses

Monday, February 05 | 5:00PM - 7:00PM
Visualizing Subcellular Compartments
Room: Mountain/Lake

Speaker 4 of 4
Joshua Hutchings, Birkbeck College, UK
Short Talk: Cryo-EM Tomography and Subtomogram Averaging of COPII Assembled on Membranes

Monday, February 05 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Monday, February 05 | 7:30PM - 10:00PM
Poster Session 1
Room: Bay


Monday, February 05 | 8:00PM - 9:00PM
Social Hour
Room: Bay
No registration fees are used to fund alcohol served at this function.

Tuesday, February 06 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Tuesday, February 06 | 8:00AM - 11:15AM
Translational Cryo-EM
Room: Mountain/Lake

Speaker 1 of 7
* Andrew B. Ward, The Scripps Research Institute, USA
Structure-Based Vaccine Design

Tuesday, February 06 | 8:00AM - 11:15AM
Translational Cryo-EM
Room: Mountain/Lake

Speaker 2 of 7
Tom Ceska, UCB Celltech, UK
Cryo-EM as a Tool for Structure Determination in Industry

Tuesday, February 06 | 8:00AM - 11:15AM
Translational Cryo-EM
Room: Mountain/Lake


Coffee Break

Tuesday, February 06 | 8:00AM - 11:15AM
Translational Cryo-EM
Room: Mountain/Lake

Speaker 4 of 7
Seungil Han, Pfizer Inc., USA
Applications of Cryo-EM in Small Molecule and Biologics Drug Design

Tuesday, February 06 | 8:00AM - 11:15AM
Translational Cryo-EM
Room: Mountain/Lake

Speaker 5 of 7
Christian Wiesmann, Novartis, Switzerland
Cryo-Em: A New Tool for Drug Discovery at Novartis

Tuesday, February 06 | 8:00AM - 11:15AM
Translational Cryo-EM
Room: Mountain/Lake

Speaker 6 of 7
Gregory Martin, Oregon Health & Science University, USA
Short Talk: Anti-Diabetic Drug Binding Site in KATP Channels Revealed by Cryo-EM

Tuesday, February 06 | 8:00AM - 11:15AM
Translational Cryo-EM
Room: Mountain/Lake

Speaker 7 of 7
Chitra Shintre, University of Oxford, UK
Short Talk: Structure of the Polycistic Kidney Disease TRP Channel PC2

Tuesday, February 06 | 11:15AM - 5:00PM
On Own for Lunch


Tuesday, February 06 | 11:15AM - 1:00PM
Poster Setup
Room: Bay


Tuesday, February 06 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 1 of 10
* Iban Ubarretxena, Icahn School of Medicine at Mount Sinai, USA
Structures of Key Intermediates in the Human Mitochondrial Chaperonin Reaction Cycle

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 2 of 10
Anika L. Burrell, University of Washington, USA
Human IMPDH1 Retinal Degeneration Mutations Promote Polymer Assembly

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 3 of 10
Joel Butterwick, Rockefeller University, USA
Cryo-EM Structure of Orco: The Molecular Basis for Insect Olfaction

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 4 of 10
Ziao Fu, Columbia University, USA
Lipid Bilayer Structure in Native Cell Membrane Nanoparticles of Multidrug Exporter AcrB

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 5 of 10
Jonas Barandun, Rockefeller University, USA
Structure of the Earliest Stable Precursor of the Ribosome - the Small Subunit Processome

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 6 of 10
Vignesh Kasinath, University of California, Berkeley, USA
Cryo-EM Structure of the Full Human PRC2 and Regulation by AEBP2 and JARID2

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 7 of 10
Qianhui Qu, Stanford University, USA
Structure of a Histone H3K4 Methyltransferase COMPASS

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 8 of 10
Julia Brasch, Columbia University, USA
Assembly of Clustered Protocadherin Neuronal Recognition Complexes Studied by X-ray Crystallography and Cryo-electron Tomography

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 9 of 10
Cristina Puchades, The Scripps Research Institute, USA
Hand-Over-Hand: How ATP Drives Protein Translocation

Tuesday, February 06 | 2:30PM - 4:30PM
Workshop 1: Specimen Preparation and Troubleshooting for Sin
gle-Particle Work
Room: Mountain/Lake

Speaker 10 of 10
Harry Low, Imperial College, UK
Architecture of a Bacterial Type II Secretion System

Tuesday, February 06 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Tuesday, February 06 | 5:00PM - 7:00PM
Frontiers in Cryo-EM
Room: Mountain/Lake

Speaker 1 of 4
Andrej Sali, University of California, San Francisco, USA
Integrative Modeling of Biomolecular Assembly Structures and Pathways

Tuesday, February 06 | 5:00PM - 7:00PM
Frontiers in Cryo-EM
Room: Mountain/Lake

Speaker 2 of 4
* Sharon Grayer Wolf, Weizmann Institute of Science, Israel
Deep Views into Vitrified Cells by Cryo-Scanning Transmission Electron Tomography

Tuesday, February 06 | 5:00PM - 7:00PM
Frontiers in Cryo-EM
Room: Mountain/Lake

Speaker 3 of 4
David Baker, University of Washington, USA
De novo Design of Self-Assembling Protein Nano Materials

Tuesday, February 06 | 5:00PM - 7:00PM
Frontiers in Cryo-EM
Room: Mountain/Lake

Speaker 4 of 4
Yuxi Liu, University of California, Los Angeles, USA
Short Talk: Near-Atomic Resolution Cryo-EM for a Small Protein on a Symmetric Imaging Scaffold

Tuesday, February 06 | 7:00PM - 8:00PM
Dinner
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Tuesday, February 06 | 7:30PM - 10:00PM
Poster Session 2
Room: Bay


Tuesday, February 06 | 8:00PM - 9:00PM
Social Hour
Room: Bay


Wednesday, February 07 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Wednesday, February 07 | 8:00AM - 11:00AM
Cryo-EM of Small Single Particles
Room: Mountain/Lake

Speaker 1 of 7
Dmitry Lyumkis, The Salk Institute for Biological Studies, USA
High-Resolution Cryo-EM Studies of Retroviral Intasomes

Wednesday, February 07 | 8:00AM - 11:00AM
Cryo-EM of Small Single Particles
Room: Mountain/Lake

Speaker 2 of 7
Maofu Liao, Harvard Medical School, USA
Single Particle Cryo-EM Studies of Lipid-Protein Interactions

Wednesday, February 07 | 8:00AM - 11:00AM
Cryo-EM of Small Single Particles
Room: Mountain/Lake


Coffee Break

Wednesday, February 07 | 8:00AM - 11:00AM
Cryo-EM of Small Single Particles
Room: Mountain/Lake

Speaker 4 of 7
* Gabriel C. Lander, The Scripps Research Institute, USA
How Low Can You Go? Size and Resolution Limits using Conventional Cryo-EM

Wednesday, February 07 | 8:00AM - 11:00AM
Cryo-EM of Small Single Particles
Room: Mountain/Lake

Speaker 5 of 7
Moran Shalev-Benami, Stanford University, USA
Short Talk: Cryo-EM Structure of the Synaptic Wiring Protein Teneurin

Wednesday, February 07 | 8:00AM - 11:00AM
Cryo-EM of Small Single Particles
Room: Mountain/Lake

Speaker 6 of 7
Christopher P. Hill, University of Utah, USA
Short Talk: Structural Basis for Protein Translocation by the Vps4 AAA ATPase

Wednesday, February 07 | 8:00AM - 11:00AM
Cryo-EM of Small Single Particles
Room: Mountain/Lake

Speaker 7 of 7
Daniel Southworth, University of California, San Francisco, USA
Short Talk: Visualizing the Substrate Translocation Mechanism of the Hsp104 AAA+ Disaggregase

Wednesday, February 07 | 11:00AM - 5:00PM
On Own for Lunch


Wednesday, February 07 | 11:00AM - 1:00PM
Poster Setup
Room: Bay


Wednesday, February 07 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Wednesday, February 07 | 2:30PM - 4:30PM
Workshop 2: Modeling and Refining Structures of Macromolecul
es in Cryo-EM Maps
Room: Mountain/Lake

Speaker 1 of 7
* Gunnar Schroeder, Forschungszentrum Julich, Germany
Fibril Structure of Amyloid-ß(1-42) by Cryo-Electron Microscopy

Wednesday, February 07 | 2:30PM - 4:30PM
Workshop 2: Modeling and Refining Structures of Macromolecul
es in Cryo-EM Maps
Room: Mountain/Lake

Speaker 2 of 7
Jane S. Richardson, Duke University, USA
CaBLAM - A New Tool Designed to Validate 2.5-4Å Models from CryoEM

Wednesday, February 07 | 2:30PM - 4:30PM
Workshop 2: Modeling and Refining Structures of Macromolecul
es in Cryo-EM Maps
Room: Mountain/Lake

Speaker 3 of 7
Doo Nam Kim, Los Alamos National Laboratory, USA
CryoFIT: Flexible Fitting of Biomolecule Structures from Cryo-EM Maps with User-Friendly Molecular Dynamics Simulation

Wednesday, February 07 | 2:30PM - 4:30PM
Workshop 2: Modeling and Refining Structures of Macromolecul
es in Cryo-EM Maps
Room: Mountain/Lake

Speaker 4 of 7
Jianpeng Ma, Baylor College of Medicine, USA
Application of Parallel Continuous Simulated Tempering to Cryo-EM Structural Refinement at Near-Atomic Resolution

Wednesday, February 07 | 2:30PM - 4:30PM
Workshop 2: Modeling and Refining Structures of Macromolecul
es in Cryo-EM Maps
Room: Mountain/Lake

Speaker 5 of 7
Abhishek Singharoy, Arizona State University, USA
Molecular Dynamics Flexible Fitting at sub-5 Å Resolution

Wednesday, February 07 | 2:30PM - 4:30PM
Workshop 2: Modeling and Refining Structures of Macromolecul
es in Cryo-EM Maps
Room: Mountain/Lake

Speaker 6 of 7
Till Rudack, Ruhr University Bochum, Germany
ModelMaker: An Interactive Integrative Modeling Tool to Build Complete Macromolecular Machines

Wednesday, February 07 | 2:30PM - 4:30PM
Workshop 2: Modeling and Refining Structures of Macromolecul
es in Cryo-EM Maps
Room: Mountain/Lake

Speaker 7 of 7
Kelly Brock, Harvard Medical School, USA
Using Evolutionary Couplings to Help Build and Refine Cryo-EM Models

Wednesday, February 07 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Wednesday, February 07 | 5:00PM - 7:15PM
Cryo-EM of Large Macromolecular Assemblies
Room: Mountain/Lake

Speaker 1 of 4
Kiyoshi Nagai, Medical Research Council, UK
Cryo-EM Snapshots of the Spliceosome Provide Insights into the Mechanism of pre-mRNA Splicing


Wednesday, February 07 | 5:00PM - 7:15PM
Cryo-EM of Large Macromolecular Assemblies
Room: Mountain/Lake

Speaker 2 of 4
Gira Bhabha, New York University School of Medicine, USA
Architectures of Lipid Transport Systems in Bacteria

Wednesday, February 07 | 5:00PM - 7:15PM
Cryo-EM of Large Macromolecular Assemblies
Room: Mountain/Lake

Speaker 3 of 4
* Adam Frost, University of California, San Francisco, USA
Function Follows Form: Cryo-EM Insights into Cell Biology

Wednesday, February 07 | 5:00PM - 7:15PM
Cryo-EM of Large Macromolecular Assemblies
Room: Mountain/Lake

Speaker 4 of 4
Eva Nogales, University of California, Berkeley, USA
Challenges and Progress in the Study of Large Human Complexes in Gene Expression Regulation

Wednesday, February 07 | 7:15PM - 8:15PM
Dinner
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Wednesday, February 07 | 7:30PM - 10:00PM
Poster Session 3
Room: Bay


Wednesday, February 07 | 8:00PM - 9:00PM
Social Hour
Room: Bay


Thursday, February 08 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Thursday, February 08 | 8:00AM - 11:15AM
Cryo-EM of Membrane Proteins
Room: Mountain/Lake

Speaker 1 of 7
* Georgios Skiniotis, Stanford University, USA
Cryo-EM Visualization of G Protein-Coupled Receptors

Thursday, February 08 | 8:00AM - 11:15AM
Cryo-EM of Membrane Proteins
Room: Mountain/Lake

Speaker 2 of 7
Judy Hirst, University of Cambridge, UK
CryoEM Structure of Mammalian Respiratory Complex I, an Asymmetric 1 MDa Energy-Converting Enzyme

Thursday, February 08 | 8:00AM - 11:15AM
Cryo-EM of Membrane Proteins
Room: Mountain/Lake


Coffee Break

Thursday, February 08 | 8:00AM - 11:15AM
Cryo-EM of Membrane Proteins
Room: Mountain/Lake

Speaker 4 of 7
Doreen Matthies, National Institutes of Health, USA
Single Particle Cryo-EM of a 200 kDa Magnesium Ion Channel

Thursday, February 08 | 8:00AM - 11:15AM
Cryo-EM of Membrane Proteins
Room: Mountain/Lake

Speaker 5 of 7
Eric Gouaux, Oregon Health & Science University, USA
Structure and Mechanism of AMPA Receptor - Auxiliary Protein Complexes

Thursday, February 08 | 8:00AM - 11:15AM
Cryo-EM of Membrane Proteins
Room: Mountain/Lake

Speaker 6 of 7
Kei Saotome, The Scripps Research Institute, USA
Short Talk: Structure of the Mechanically Activated Ion Channel Piezo1

Thursday, February 08 | 8:00AM - 11:15AM
Cryo-EM of Membrane Proteins
Room: Mountain/Lake

Speaker 7 of 7
Luke H. Chao, Massachusetts General Hospital, USA
Short Talk: Single Particle Cryo-EM Studies of Mitochondrial Inner-Membrane Fusion

Thursday, February 08 | 11:15AM - 5:00PM
On Own for Lunch


Thursday, February 08 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Thursday, February 08 | 5:00PM - 6:00PM
Recent Technological Advances in Cryo EM
Room: Mountain/Lake

Speaker 1 of 2
Radostin Danev, University of Tokyo, Japan
Single Particle Imaging with the Volta Phase Plate

Thursday, February 08 | 5:00PM - 6:00PM
Recent Technological Advances in Cryo EM
Room: Mountain/Lake

Speaker 2 of 2
* Bridget Carragher, New York Structural Biology Center, USA
Sample Vitrification Issues and Some Ideas about How to Overcome Them

Thursday, February 08 | 6:00PM - 6:45PM
Closing Keynote Address
Room: Mountain/Lake

Speaker 1 of 2
* Georgios Skiniotis, Stanford University, USA

Thursday, February 08 | 6:00PM - 6:45PM
Closing Keynote Address
Room: Mountain/Lake

Speaker 2 of 2
Joachim Frank, Columbia University, USA
Story in a Sample: Multiple Ribosome Structures in a Single Cryo-EM Experiment

Thursday, February 08 | 6:45PM - 7:00PM
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Room: Mountain/Lake


Thursday, February 08 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Thursday, February 08 | 7:00PM - 8:00PM
Social Hour
Room: Granhall


Thursday, February 08 | 8:00PM - 11:00PM
Entertainment
Room: Granhall
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Thursday, February 08 | 8:00PM - 11:00PM
Cash Bar
Room: Granhall


Friday, February 09 | 10:24AM - 10:24AM
Departure


*Session Chair
†Invited, not yet responded.