Past Meeting | Keystone Symposia

This meeting took place in the past. Here is a list of meetings that are related:
		Engineering the Genome (2020Q2)

Web Desc
Genome Engineering: From Mechanisms to Therapies Organizer(s): Andrew May, Rodolphe Barrangou and Knut Woltjen Date: February 19 - 23, 2019 Location: Fairmont Empress Victoria / Victoria Conference Centre, Victoria, BC, Canada Sponsored by BlueRock Therapeutics, Editas Medicine, Thermo Fisher Scientific Inc. and Vertex Pharmaceuticals Incorporated Summary of Meeting: The use of programmable nucleases such as CRISPR-Cas systems, ZFNs and TALENs has revolutionized cell biology by providing the ability to manipulate specific genetic and epigenetic states within living cells. These systems have been broadly applied as tools in research settings and increasingly are being developed to create improved models of disease and engineer cells for therapeutic purposes. Together with other DNA modifying systems such as recombinases, integrases and transposases, it is now possible to introduce mutations that will model human disease, build complex synthetic signaling networks to perform regulated functions and design cells to target specific disease states. Improvements to the methods involved requires understanding enzyme structures and mechanisms and how they intersect with cellular DNA repair systems. The intersection of this basic science with engineering approaches and improved cellular models is revolutionizing our understanding and treatment of human disease. The goal of this Keystone Symposium is to bring together people developing and studying genome engineering tools with groups who are applying them to build new disease models, identify disease mechanisms and drug targets, and develop cell-based therapeutics and genetic medicines. In addition to covering engineering of human and animal cells, this meeting will also highlight the emerging field of genome engineering to identify new anti-microbial and anti-viral drugs and applications towards next generation antibiotics. Invited talks will explore a broad range of topics covering new technologies, fundamental basic research, through the development of screening approaches, stem cell-based models of disease and design and development of cellular therapeutics. Scholarship Deadline: October 23 2018 Discounted Abstract Deadline: October 23 2018 Abstract Deadline: November 20 2018 Discounted Registration Deadline: December 19 2018 Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting: We gratefully acknowledge additional support from these exhibitors at this conference: We gratefully acknowledge additional support for this conference from: We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements: bluebird bio $10,000 - $24,999 We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising: We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising: Click here to view more of these organizations Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds: Click here to view more of these organizations

Web Desc

Genome Engineering: From Mechanisms to Therapies

Organizer(s): Andrew May, Rodolphe Barrangou and Knut Woltjen

Date: February 19 - 23, 2019
Location: Fairmont Empress Victoria / Victoria Conference Centre, Victoria, BC, Canada

Sponsored by BlueRock Therapeutics, Editas Medicine, Thermo Fisher Scientific Inc. and Vertex Pharmaceuticals Incorporated

Summary of Meeting:

The use of programmable nucleases such as CRISPR-Cas systems, ZFNs and TALENs has revolutionized cell biology by providing the ability to manipulate specific genetic and epigenetic states within living cells. These systems have been broadly applied as tools in research settings and increasingly are being developed to create improved models of disease and engineer cells for therapeutic purposes. Together with other DNA modifying systems such as recombinases, integrases and transposases, it is now possible to introduce mutations that will model human disease, build complex synthetic signaling networks to perform regulated functions and design cells to target specific disease states. Improvements to the methods involved requires understanding enzyme structures and mechanisms and how they intersect with cellular DNA repair systems. The intersection of this basic science with engineering approaches and improved cellular models is revolutionizing our understanding and treatment of human disease. The goal of this Keystone Symposium is to bring together people developing and studying genome engineering tools with groups who are applying them to build new disease models, identify disease mechanisms and drug targets, and develop cell-based therapeutics and genetic medicines. In addition to covering engineering of human and animal cells, this meeting will also highlight the emerging field of genome engineering to identify new anti-microbial and anti-viral drugs and applications towards next generation antibiotics. Invited talks will explore a broad range of topics covering new technologies, fundamental basic research, through the development of screening approaches, stem cell-based models of disease and design and development of cellular therapeutics.

Scholarship Deadline: October 23 2018

Discounted Abstract Deadline: October 23 2018

Abstract Deadline: November 20 2018

Discounted Registration Deadline: December 19 2018

Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:

We gratefully acknowledge additional support from these exhibitors at this conference:

We gratefully acknowledge additional support for this conference from:

We gratefully acknowledge additional in-kind support for this conference from those foregoing speaker expense reimbursements:

bluebird bio

$10,000 - $24,999

We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations

Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program


Tuesday, February 19 \| 4:00PM - 8:00PM Arrival and Registration Room: Palm Court
Tuesday, February 19 \| 6:00PM - 8:00PM Welcome Mixer Room: Palm Court No registration fees are used to fund alcohol served at this function.
Wednesday, February 20 \| 7:30AM - 8:30AM Breakfast Room: Palm Court
Wednesday, February 20 \| 8:30AM - 9:30AM Welcome and Keynote Address Room: Salon A Speaker 1 of 2 * Andrew May, Sana Biotechnology, Inc., USA
Wednesday, February 20 \| 8:30AM - 9:30AM Welcome and Keynote Address Room: Salon A Speaker 2 of 2 Philip D. Gregory, Bluebird Bio, USA Toward Therapeutic Genome Editing: A Brief History (And Some Lessons Learned from Classical Gene Therapy)
Wednesday, February 20 \| 9:30AM - 11:45AM Genome Engineering Tools and Technologies Room: Salon A Speaker 1 of 6 * Dana Carroll, University of Utah School of Medicine, USA
Wednesday, February 20 \| 9:30AM - 11:45AM Genome Engineering Tools and Technologies Room: Salon A Speaker 2 of 6 Prashant Mali, University of California, San Diego, USA Therapeutic Strategies via Genome Engineering: New Approaches and New Challenges
Wednesday, February 20 \| 9:30AM - 11:45AM Genome Engineering Tools and Technologies Room: Salon A Coffee Break
Wednesday, February 20 \| 9:30AM - 11:45AM Genome Engineering Tools and Technologies Room: Salon A Speaker 4 of 6 Charles Gersbach, Duke University, USA Epigenome Editing for Gene Therapy, Cell Programming and Functional Epigenomics
Wednesday, February 20 \| 9:30AM - 11:45AM Genome Engineering Tools and Technologies Room: Salon A Speaker 5 of 6 Alexis C. Komor, University of California, San Diego, USA Using Uracil as a Genome Editing Intermediate
Wednesday, February 20 \| 9:30AM - 11:45AM Genome Engineering Tools and Technologies Room: Salon A Speaker 6 of 6 Peter Cameron, Caribou Biosciences, Inc., USA Short Talk: Harnessing Type I CRISPR–Cas Systems for Human Genome Engineering
Wednesday, February 20 \| 11:45AM - 5:00PM On Own for Lunch
Wednesday, February 20 \| 11:45AM - 1:00PM Poster Setup Room: Salon B/C
Wednesday, February 20 \| 1:00PM - 10:00PM Poster Viewing Room: Salon B/C
Wednesday, February 20 \| 2:30PM - 4:30PM Workshop 1: Tools and Technologies Speaker 1 of 7 * Joanne Kamens, Addgene, USA
Wednesday, February 20 \| 2:30PM - 4:30PM Workshop 1: Tools and Technologies Speaker 2 of 7 Abigail R. Lambert, Fred Hutchinson Cancer Research Center, USA Structural and Functional Properties of Wild-Type and Engineered Meganucleases and MegaTALs
Wednesday, February 20 \| 2:30PM - 4:30PM Workshop 1: Tools and Technologies Speaker 3 of 7 Henriette O'Geen, University of California, Davis, USA Engineering Epigenetic Memory Requires Co-Targeting of Histone Methylatransferases and DNA Methylatransferases
Wednesday, February 20 \| 2:30PM - 4:30PM Workshop 1: Tools and Technologies Speaker 4 of 7 Alister Funnell, Altius Institute for Biomedical Sciences, USA Rapid Single-Cell Quantification of On- and Off-Target Nuclease Activity
Wednesday, February 20 \| 2:30PM - 4:30PM Workshop 1: Tools and Technologies Speaker 5 of 7 Janin Grajcarek, Kyoto University, Japan Identification of Microhomology-Flanked Deletion Mutations Across the Human Genome Enables Efficient Creation of Isogenic Disease Models in hiPSCs by CRISPR/Cas9
Wednesday, February 20 \| 2:30PM - 4:30PM Workshop 1: Tools and Technologies Speaker 6 of 7 Hien Bao Dieu Thai, Korea Institute of Science and Technology, South Korea DNAzymes-Based Tetrahedral Nanostructure for Enhanced Intracellular Gene-Silencing Activity
Wednesday, February 20 \| 2:30PM - 4:30PM Workshop 1: Tools and Technologies Speaker 7 of 7 Brock Roberts, Allen Institute for Cell Science, USA Systematic Gene Tagging to Illuminate Stem Cell Organization
Wednesday, February 20 \| 4:30PM - 5:00PM Coffee Available Room: Prefunction 2
Wednesday, February 20 \| 5:00PM - 7:00PM Structure and Mechanism of Genome Editing Systems Room: Salon A Speaker 1 of 5 * Maria Jasin, Memorial Sloan Kettering Cancer Center, USA
Wednesday, February 20 \| 5:00PM - 7:00PM Structure and Mechanism of Genome Editing Systems Room: Salon A Speaker 2 of 5 Benjamin P. Kleinstiver, Massachusetts General Hospital, USA Engineered CRISPR Nucleases to Enhance Genome Editing
Wednesday, February 20 \| 5:00PM - 7:00PM Structure and Mechanism of Genome Editing Systems Room: Salon A Speaker 3 of 5 Osamu Nureki, University of Tokyo, Japan Molecular Mechanism of CRISPR and Structure-Based Development of Genome Editing Tool toward Medical Applications
Wednesday, February 20 \| 5:00PM - 7:00PM Structure and Mechanism of Genome Editing Systems Room: Salon A Speaker 4 of 5 Edward J. Rebar, Sangamo Therapeutics, Inc., USA Short Talk: Optimizing Nuclease Specificity for Gene Editing via Tuning of Cleavage Kinetics Enables Complete Gene Modification with No Detectable Off-Targets
Wednesday, February 20 \| 5:00PM - 7:00PM Structure and Mechanism of Genome Editing Systems Room: Salon A Speaker 5 of 5 Amit Choudhary, Harvard Medical School, USA Short Talk: Synthetic Activators, Inhibitors and Degraders of CRISPR-Associated Nucleases
Wednesday, February 20 \| 7:00PM - 8:00PM Social Hour with Lite Bites Room: Salon B/C No registration fees are used to fund alcohol served at this function.
Wednesday, February 20 \| 7:30PM - 10:00PM Poster Session 1 Room: Salon B/C
Thursday, February 21 \| 7:30AM - 8:30AM Breakfast Room: Palm Court
Thursday, February 21 \| 8:00AM - 8:30AM Poster Setup Room: Salon B/C
Thursday, February 21 \| 8:30AM - 5:00PM Poster Viewing Room: Salon B/C
Thursday, February 21 \| 8:30AM - 11:30AM Engineered Models of Genetic Disease Room: Salon A Speaker 1 of 7 * Bruce R. Conklin, University of California, San Francisco, USA
Thursday, February 21 \| 8:30AM - 11:30AM Engineered Models of Genetic Disease Room: Salon A Speaker 2 of 7 Danwei Huangfu, Memorial Sloan Kettering Cancer Institute, USA Human Pluripotent Stem Cells as a Genetic Model for Human Development and Disease
Thursday, February 21 \| 8:30AM - 11:30AM Engineered Models of Genetic Disease Room: Salon A Speaker 3 of 7 Knut Woltjen, CiRA, Kyoto University, Japan Precise Human Disease Allele Creation and Correction through Microhomology-Mediated End Joining
Thursday, February 21 \| 8:30AM - 11:30AM Engineered Models of Genetic Disease Room: Salon A Coffee Break
Thursday, February 21 \| 8:30AM - 11:30AM Engineered Models of Genetic Disease Room: Salon A Speaker 5 of 7 Amy J. Wagers, Harvard University, USA Gene Editing in Stem Cells
Thursday, February 21 \| 8:30AM - 11:30AM Engineered Models of Genetic Disease Room: Salon A Speaker 6 of 7 Erika Sasaki, Central Institute for Experimental Animals, Japan Development of Genetically Modified Non-Human Primate Disease Models
Thursday, February 21 \| 8:30AM - 11:30AM Engineered Models of Genetic Disease Room: Salon A Speaker 7 of 7 Diogo Mosqueira, University of Nottingham, UK Short Talk: CRISPR/Cas9 Genome Editing in Human Pluripotent Stem Cell-Cardiomyocytes to Model and Treat Hypertrophic Cardiomyopathy
Thursday, February 21 \| 11:30AM - 12:30PM Lunch Room: Salon B/C
Thursday, February 21 \| 12:00PM - 2:30PM Poster Session 2 Room: Salon B/C
Thursday, February 21 \| 2:30PM - 3:00PM Coffee Available Room: Prefunction 2
Thursday, February 21 \| 3:00PM - 5:00PM Design and Engineering of Cellular Devices Room: Salon A Speaker 1 of 5 * Alexis C. Komor, University of California, San Diego, USA
Thursday, February 21 \| 3:00PM - 5:00PM Design and Engineering of Cellular Devices Room: Salon A Speaker 2 of 5 Wilson Wong, Boston University, USA Mammalian Cell Design Using Synthetic Biology
Thursday, February 21 \| 3:00PM - 5:00PM Design and Engineering of Cellular Devices Room: Salon A Speaker 3 of 5 Timothy K. Lu, Massachusetts Institute of Technology, USA Synthetic Gene Circuits for Next-Generation Therapeutics
Thursday, February 21 \| 3:00PM - 5:00PM Design and Engineering of Cellular Devices Room: Salon A Speaker 4 of 5 Zoltan Ivics, Paul Ehrlich Institute, Germany Transposons: Molecular Parasites Tamed for Advanced Genome Engineering
Thursday, February 21 \| 3:00PM - 5:00PM Design and Engineering of Cellular Devices Room: Salon A Speaker 5 of 5 Yale S. Michaels, University of Oxford, UK Short Talk: A Generalizable Method for Precisely Tuning Gene Expression Levels in Mammalian Cells with Engineered MicroRNA Target Sites
Thursday, February 21 \| 5:00PM - 5:00PM On Own for Dinner
Friday, February 22 \| 7:30AM - 8:30AM Breakfast Room: Palm Court
Friday, February 22 \| 8:30AM - 11:30AM Genome Editing Screens for Function and Disease Mechanisms Room: Salon A Speaker 1 of 6 * Danwei Huangfu, Memorial Sloan Kettering Cancer Institute, USA
Friday, February 22 \| 8:30AM - 11:30AM Genome Editing Screens for Function and Disease Mechanisms Room: Salon A Speaker 2 of 6 Fyodor D. Urnov, University of California, Berkeley, USA Editing Human Genome Control Circuits to Reveal Disease Mechanisms and Targets for Intervention in the Clinic
Friday, February 22 \| 8:30AM - 11:30AM Genome Editing Screens for Function and Disease Mechanisms Room: Salon A Speaker 3 of 6 Jan E. Carette, Stanford University, USA CRISPR-Cas Screens for Studying Virus-Host Interactions
Friday, February 22 \| 8:30AM - 11:30AM Genome Editing Screens for Function and Disease Mechanisms Room: Salon A Coffee Break
Friday, February 22 \| 8:30AM - 11:30AM Genome Editing Screens for Function and Disease Mechanisms Room: Salon A Speaker 5 of 6 Nozomu Yachie, University of Tokyo, Japan Tracing Dynamics of Cells and Molecules using DNA Barcodes and Genome Editing
Friday, February 22 \| 8:30AM - 11:30AM Genome Editing Screens for Function and Disease Mechanisms Room: Salon A Speaker 6 of 6 Britt S. Adamson, Princeton University, USA Mapping the Processes of Genome Editing with High-Resolution Functional Genomics
Friday, February 22 \| 11:30AM - 12:30PM Take the Bull by the Horns: Steps to a Fulfilling Career in Science Speaker 1 of 1 * Joanne Kamens, Addgene, USA
Friday, February 22 \| 11:30AM - 5:00PM On Own for Lunch
Friday, February 22 \| 4:30PM - 5:00PM Coffee Available Room: Prefunction 2
Friday, February 22 \| 5:00PM - 7:15PM Harnessing DNA Repair Mechanisms for Genome Engineering Room: Salon A Speaker 1 of 6 * Britt S. Adamson, Princeton University, USA
Friday, February 22 \| 5:00PM - 7:15PM Harnessing DNA Repair Mechanisms for Genome Engineering Room: Salon A Speaker 2 of 6 Maria Jasin, Memorial Sloan Kettering Cancer Center, USA Homologous Recombination and End-Joining Mechanisms in Genome Editing
Friday, February 22 \| 5:00PM - 7:15PM Harnessing DNA Repair Mechanisms for Genome Engineering Room: Salon A Speaker 3 of 6 Beeke Wienert, University of California, San Francisco, USA Unbiased Detection of CRISPR Off-Targets in vivo using DISCOVER-Seq
Friday, February 22 \| 5:00PM - 7:15PM Harnessing DNA Repair Mechanisms for Genome Engineering Room: Salon A Speaker 4 of 6 Nancy Maizels, University of Washington School of Medicine, USA Gene Correction at Targeted DNA Breaks
Friday, February 22 \| 5:00PM - 7:15PM Harnessing DNA Repair Mechanisms for Genome Engineering Room: Salon A Speaker 5 of 6 Andrew May, Sana Biotechnology, Inc., USA DNA Repair Outcomes Provide Insight into Genome Editing Mechanisms in Primary Cell Systems
Friday, February 22 \| 5:00PM - 7:15PM Harnessing DNA Repair Mechanisms for Genome Engineering Room: Salon A Speaker 6 of 6 Tetsushi Sakuma, Hiroshima University, Japan Short Talk: Concurrent MMEJ-Assisted Fusional Knock-In of Long Gene Cassette in Human Cells
Friday, February 22 \| 7:15PM - 8:15PM Social Hour with Lite Bites Room: Salon C No registration fees are used to fund alcohol served at this function.
Saturday, February 23 \| 7:30AM - 8:30AM Breakfast Room: Palm Court
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Speaker 1 of 8 * Andrew May, Sana Biotechnology, Inc., USA
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Speaker 2 of 8 Rodolphe Barrangou, North Carolina State University, USA Engineering Lactobacilli for Human Health Applications
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Speaker 3 of 8 Jason M. Peters, University of Wisconsin-Madison, USA Bacterial CRISPRi Screens to Identify the Mode of Action of Novel Antibiotics
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Coffee Break
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Speaker 5 of 8 Richard P. Novick, New York University, USA Conversion of Staphylococcal Pathogenicity Islands to CRISPR-Cas9-Based Antibacterial Drones
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Speaker 6 of 8 Joel Berry, Caribou Biosciences, USA Short Talk: Utilizing CRISPR-Based Genome Editing for Microbiome Engineering
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Speaker 7 of 8 Akos Nyerges, Hungarian Academy of Sciences, Hungary Short Talk: Predicting Antibiotic Resistance by Targeted Mutagenesis and Directed Evolution in Pathogenic Bacteria
Saturday, February 23 \| 8:30AM - 11:30AM Genome Engineering of Bacteria for Therapeutic and Diagnosti c Applications Room: Salon A Speaker 8 of 8 David R. Edgell, University of Western Ontario, Canada Short Talk: High Efficiency Inter-Species Conjugative Transfer of a CRISPR Nuclease for Targeted Bacterial Elimination
Saturday, February 23 \| 11:30AM - 5:00PM On Own for Lunch
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 1 of 9 * Shondra M. Pruett-Miller, St. Jude Children’s Research Hospital, USA
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 2 of 9 Dana V. Foss, University of California, Berkeley, USA Engineering Cas9 for T-Cell Specific Uptake and Therapeutic Genome Editing
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 3 of 9 Jacquelyn L S Hanson, SQZ Biotechnologies, USA Microfluidic Delivery of Bioactive Molecules via SQZ Platform Enables Efficient T Cell Genome Engineering with Preserved Functionality
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 4 of 9 Lauren Elizabeth Woodard, Vanderbilt University, USA Less Is More: How Less Recombinase Expression Produces More Genome-Modified Cells
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 5 of 9 Taisuke Kato, Niigata University, Japan Gene Therapy for DRPLA Model Mice by AAV-Delivered CRISPR / Cas9
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 6 of 9 Eric Aird, University of Minnesota, USA Enhancing HDR Efficiency by Tethering DNA to Cas9 via a Fused HUH Endonuclease
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 7 of 9 Erin Morgan, University of California, Santa Barbara, USA Controlling the Genome: Light-Activated Delivery of Gene Editing Proteins and siRNA Allows for Up and Down Regulation of the Genome using Hollow Gold Nanoparticles
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 8 of 9 Masato Ohtsuka, Tokai University, Japan i-GONAD: A Method for Generation of Genome-Edited Rodents without ex vivo Handling of Embryos
Saturday, February 23 \| 2:30PM - 4:30PM Workshop 2: Delivery Methods Room: Salon A A collection of short talks chosen from the abstracts describing methods and approaches for in vitro and in vivo delivery of genome engineering technologies. The workshop would cover topics including devices for electroporation, mechanical introduction of molecules into cells, cell-specific targeting, viruses and viral-like particles, nanoparticles to address the key need to develop delivery vehicles for effective use of genome engineering approaches in therapeutics and research models. Speaker 9 of 9 Anthony L. Forget, Intellia Therapeutics, USA Supra-Therapeutic Levels of Transgene Expression Achieved in vivo by CRISPR/Cas9 Mediated Targeted Gene Insertion
Saturday, February 23 \| 4:30PM - 5:00PM Coffee Available Room: Prefunction 2
Saturday, February 23 \| 5:00PM - 7:15PM Genome Editing for Treating Human Disease Room: Salon A Speaker 1 of 6 * Fyodor D. Urnov, University of California, Berkeley, USA
Saturday, February 23 \| 5:00PM - 7:15PM Genome Editing for Treating Human Disease Room: Salon A Speaker 2 of 6 Bruce R. Conklin, University of California, San Francisco, USA Using Patient-Derived iPSC Tissues to Model Precise Genome Surgery
Saturday, February 23 \| 5:00PM - 7:15PM Genome Editing for Treating Human Disease Room: Salon A Speaker 3 of 6 Leonela Amoasii, Exonics Therapeutics, USA Gene Editing Restores Dystrophin Expression in a Canine Model of Duchenne Muscular Dystrophy
Saturday, February 23 \| 5:00PM - 7:15PM Genome Editing for Treating Human Disease Room: Salon A Speaker 4 of 6 Lukas Jeker, University of Basel, Switzerland Short Talk: Repairing Foxp3 Mutations in T Cells Restores Regulatory T Cell Function
Saturday, February 23 \| 5:00PM - 7:15PM Genome Editing for Treating Human Disease Room: Salon A Speaker 5 of 6 Jorge Mansilla-Soto, Memorial Sloan Kettering Cancer Center, USA Advancing CAR-T Therapy with Precise Genome Engineering
Saturday, February 23 \| 5:00PM - 7:15PM Genome Editing for Treating Human Disease Room: Salon A Speaker 6 of 6 Vic E. Myer, Atlas Ventures, USA Controlling Rearrangement Frequencies in the Context of Multigene Genome Editing
Saturday, February 23 \| 7:15PM - 7:30PM Meeting Wrap-Up: Outcomes and Future Directions (Organizers) Room: Salon A
Saturday, February 23 \| 7:30PM - 8:30PM Social Hour with Lite Bites Room: Palm Court/Crystal Ballroom No registration fees are used to fund alcohol served at this function.
Saturday, February 23 \| 8:00PM - 11:00PM Entertainment Room: Palm Court/Crystal Ballroom Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.
Saturday, February 23 \| 8:00PM - 11:00PM Cash Bar Room: Palm Court/Crystal Ballroom
Sunday, February 24 \| 10:24AM - 10:24AM Departure *Session Chair.