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Web Desc
Imaging Across Scales: Leveraging the Revolution in Resolution Organizer(s): Bo Huang and Elizabeth R. Wright Date: April 07 - 10, 2019 Location: Snowbird Resort, Snowbird, UT, USA Supported by the Directors' Fund For important information on the coronavirus, please click here Summary of Meeting: Cellular processes are orchestrated by a large number of biomolecules in both spatially and temporally coordinated manners within a small volume. To uncover the underlying organizational principles and their functional relevance, light and electron microscopy are indispensable tools. Light microscopy is exquisitely suited for mapping the spatial localization, temporal dynamics and activity profiles of biomolecules in individual cells to tissues. Advanced electron microscopy technologies support studies for determining the high-resolution structures of isolated biomolecules, as well as biomolecule structure and location within the context of an intact cell. Recent technical breakthroughs have supported the generation of crystal clear pictures and coordination to biological function of biomolecules from the molecular scale to tissue scale. Further technical advancements and biological discoveries call for seamless integration and correlation of different microscopy modalities, labeling methods and analysis/modeling platforms, as well as close communications between technique developers and biologists. Researchers from these very different disciplines, however, do not often sit in the same room and discuss new developments and opportunities for bridging across fields. Instead, their interactions have been limited to brief encounters at sessions of large society meetings and sporadic small symposiums. This meeting aims at bringing together investigators from these research communities in order to stimulate new ideas and forge new collaborations, which will help not only to extend the frontiers of microscopy, but also to bridge new technique developments and their potential applications to addressing the complexity of cell structure and function. Scholarship Deadline: December 11 2018 Discounted Abstract Deadline: December 11 2018 Abstract Deadline: February 5 2019 Discounted Registration Deadline: February 5 2019 Abstract submission is now closed. Registered attendees may bring a poster onsite. Please contact our office at +1 800-253-0685; +1 970-262-1230 or email info@keystonesymposia.org if you are interested. We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising: Click here to view more of these organizations Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds: Click here to view more of these organizations

Web Desc

Imaging Across Scales: Leveraging the Revolution in Resolution

Organizer(s): Bo Huang and Elizabeth R. Wright

Date: April 07 - 10, 2019
Location: Snowbird Resort, Snowbird, UT, USA

Supported by the Directors' Fund

For important information on the coronavirus, please click here

Summary of Meeting:

Cellular processes are orchestrated by a large number of biomolecules in both spatially and temporally coordinated manners within a small volume. To uncover the underlying organizational principles and their functional relevance, light and electron microscopy are indispensable tools. Light microscopy is exquisitely suited for mapping the spatial localization, temporal dynamics and activity profiles of biomolecules in individual cells to tissues. Advanced electron microscopy technologies support studies for determining the high-resolution structures of isolated biomolecules, as well as biomolecule structure and location within the context of an intact cell. Recent technical breakthroughs have supported the generation of crystal clear pictures and coordination to biological function of biomolecules from the molecular scale to tissue scale. Further technical advancements and biological discoveries call for seamless integration and correlation of different microscopy modalities, labeling methods and analysis/modeling platforms, as well as close communications between technique developers and biologists. Researchers from these very different disciplines, however, do not often sit in the same room and discuss new developments and opportunities for bridging across fields. Instead, their interactions have been limited to brief encounters at sessions of large society meetings and sporadic small symposiums. This meeting aims at bringing together investigators from these research communities in order to stimulate new ideas and forge new collaborations, which will help not only to extend the frontiers of microscopy, but also to bridge new technique developments and their potential applications to addressing the complexity of cell structure and function.

Scholarship Deadline: December 11 2018

Discounted Abstract Deadline: December 11 2018

Abstract Deadline: February 5 2019

Discounted Registration Deadline: February 5 2019

Abstract submission is now closed. Registered attendees may bring a poster onsite. Please contact our office at +1 800-253-0685; +1 970-262-1230 or email info@keystonesymposia.org if you are interested.

We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations

Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program


Sunday, April 07 \| 4:00PM - 8:00PM Arrival and Registration Room: Ballroom 3 Lobby
Sunday, April 07 \| 6:00PM - 8:00PM Welcome Mixer Room: Ballroom 3 Lobby No registration fees are used to fund alcohol served at this function.
Monday, April 08 \| 7:00AM - 8:00AM Breakfast Room: Golden Cliff
Monday, April 08 \| 8:00AM - 9:00AM Welcome and Keynote Address Room: Ballroom 3 Speaker 1 of 3 * Bo Huang, University of California, San Francisco, USA
Monday, April 08 \| 8:00AM - 9:00AM Welcome and Keynote Address Room: Ballroom 3 Speaker 2 of 3 * Elizabeth R. Wright, University of Wisconsin-Madison, USA
Monday, April 08 \| 8:00AM - 9:00AM Welcome and Keynote Address Room: Ballroom 3 Speaker 3 of 3 Xiaowei Zhuang, Harvard University, USA Transcriptome and Chromosome Imaging in Single Cells
Monday, April 08 \| 9:00AM - 11:30AM Microscopy Techniques I Room: Ballroom 3 Speaker 1 of 7 * Elizabeth R. Wright, University of Wisconsin-Madison, USA
Monday, April 08 \| 9:00AM - 11:30AM Microscopy Techniques I Room: Ballroom 3 Speaker 2 of 7 Melanie Ohi, University of Michigan, USA Using Single Particle cryo-EM to Study Machines Involved in Bacterial Pathogenesis
Monday, April 08 \| 9:00AM - 11:30AM Microscopy Techniques I Room: Ballroom 3 Coffee Break
Monday, April 08 \| 9:00AM - 11:30AM Microscopy Techniques I Room: Ballroom 3 Speaker 4 of 7 Elizabeth Villa, University of California, San Diego, USA Opening Windows Into Parkinson’s Disease: Revealing the in situ Structure of a Pathogenic Mutant of LRRK2
Monday, April 08 \| 9:00AM - 11:30AM Microscopy Techniques I Room: Ballroom 3 Speaker 5 of 7 Toshio Ando, Kanazawa University, Japan High-Speed AFM
Monday, April 08 \| 9:00AM - 11:30AM Microscopy Techniques I Room: Ballroom 3 Speaker 6 of 7 Jennifer Kefauver, The Scripps Research Institute, USA Short Talk: Structure of the Human Volume-Regulated Anion Channel
Monday, April 08 \| 9:00AM - 11:30AM Microscopy Techniques I Room: Ballroom 3 Speaker 7 of 7 Anthony Fitzpatrick, Zuckerman Institute, Columbia University, USA Short Talk: Ultrafast Electron Tomography
Monday, April 08 \| 11:30AM - 5:00PM On Own for Lunch
Monday, April 08 \| 11:30AM - 1:00PM Poster Setup Room: Superior
Monday, April 08 \| 1:00PM - 10:00PM Poster Viewing Room: Superior
Monday, April 08 \| 4:30PM - 5:00PM Coffee Available Room: Ballroom Lobby
Monday, April 08 \| 5:00PM - 7:00PM Microscopy Techniques II Room: Ballroom 3 Speaker 1 of 6 * Bo Huang, University of California, San Francisco, USA
Monday, April 08 \| 5:00PM - 7:00PM Microscopy Techniques II Room: Ballroom 3 Speaker 2 of 6 Joerg Bewersdorf, Yale University, USA 3D and Multicolor Live-Cell Super-Resolution Microscopy for Cell Biological Research
Monday, April 08 \| 5:00PM - 7:00PM Microscopy Techniques II Room: Ballroom 3 Speaker 3 of 6 Manos Karagiannis, Massachusetts Institute of Technology, USA Expansion Microscopy and Other Tools for Imaging at Scale
Monday, April 08 \| 5:00PM - 7:00PM Microscopy Techniques II Room: Ballroom 3 Speaker 4 of 6 Loic A. Royer, Chan Zuckerberg Biohub, USA Pushing the Limits of Fluorescence Microscopy with Adaptive Imaging and Deep Learning
Monday, April 08 \| 5:00PM - 7:00PM Microscopy Techniques II Room: Ballroom 3 Speaker 5 of 6 Hongda Wang, University of California, Los Angeles, USA Short Talk: Cross-Modality Super-Resolution in Fluorescence Microscopy Enabled by Deep Learning
Monday, April 08 \| 5:00PM - 7:00PM Microscopy Techniques II Room: Ballroom 3 Speaker 6 of 6 Jonathan Herrmann, Stanford University, USA Short Talk: Time-Resolved Cryo-EM Reveals Multi-Step 2D Protein Crystallization via a Calcium-Mediated Conformational Switch
Monday, April 08 \| 7:00PM - 8:00PM Social Hour with Lite Bites Room: Superior Lobby No registration fees are used to fund alcohol served at this function.
Monday, April 08 \| 7:30PM - 10:00PM Poster Session 1 Room: Superior
Tuesday, April 09 \| 7:00AM - 8:00AM Breakfast Room: Golden Cliff
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 1 of 9 * Ru Gunawardane, Allen Institute for Cell Science, USA
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 2 of 9 Jin Zhang, University of California, San Diego, USA Illuminating the Biochemical Activity Architecture of the Cell
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 3 of 9 Takeshi Imai, Kyushu University, Japan Imaging Neuronal Circuits with Tissue Clearing
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 4 of 9 Matthias Wolf, Okinawa Institute of Science and Technology, Japan Short Talk: 3D Imaging of Selectively Labeled Drosophila Neurons with Serial Blockface Electron Microscopy and X-Ray Microscopy Using Genetically Encoded Tags
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Coffee Break
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 6 of 9 Clodagh C. O'Shea, The Salk Institute for Biological Studies, USA Cracking the Nucleus: Visualizing the Genome at Nucleosome Resolutions and Megabase Scales
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 7 of 9 Bo Huang, University of California, San Francisco, USA Imaging the Genome in Living Cells
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 8 of 9 Adrian R. Ferre-D'Amare, National Institutes of Health, USA Short Talk: RNA Analogs of GFP: Aptamers that Induce Fluorescence of Cognate Small-Molecule Ligands
Tuesday, April 09 \| 8:00AM - 11:15AM Molecular Probes I Room: Ballroom 3 Speaker 9 of 9 Richard Conroy, NIH Common Fund, USA Short Talk: The NIH Common Fund Human BioMolecular Atlas Program (HuBMAP)
Tuesday, April 09 \| 11:15AM - 5:00PM On Own for Lunch
Tuesday, April 09 \| 11:15AM - 1:00PM Poster Setup Room: Superior
Tuesday, April 09 \| 1:00PM - 10:00PM Poster Viewing Room: Superior
Tuesday, April 09 \| 4:30PM - 5:00PM Coffee Available Room: Ballroom Lobby
Tuesday, April 09 \| 5:00PM - 7:00PM Molecular Probes II Room: Ballroom 3 Speaker 1 of 6 * Clodagh C. O'Shea, The Salk Institute for Biological Studies, USA
Tuesday, April 09 \| 5:00PM - 7:00PM Molecular Probes II Room: Ballroom 3 Speaker 2 of 6 Ru Gunawardane, Allen Institute for Cell Science, USA Labeling Endogenous Proteins in iPSCs to Illuminate Stem Cell Organization and Dynamics
Tuesday, April 09 \| 5:00PM - 7:00PM Molecular Probes II Room: Ballroom 3 Speaker 3 of 6 Xiaokun Shu, University of California, San Francisco, USA Visualizing Dynamic Cell Signaling in vivo with a New Class of Fluorescent Reporters
Tuesday, April 09 \| 5:00PM - 7:00PM Molecular Probes II Room: Ballroom 3 Speaker 4 of 6 Gary G. Borisy, Forsyth, USA Mapping Microbiomes at the Micron Scale
Tuesday, April 09 \| 5:00PM - 7:00PM Molecular Probes II Room: Ballroom 3 Speaker 5 of 6 Micah Rapp, Columbia University in the City of New York, USA Short Talk: Visualizing the Clustered Protocadherin Self-Recognition Complex
Tuesday, April 09 \| 5:00PM - 7:00PM Molecular Probes II Room: Ballroom 3 Speaker 6 of 6 Antonina Roll-Mecak, DHHS/NINDS, National Institutes of Health, USA Short Talk: Severing Enzymes Amplify Microtubule Arrays by Promoting Microtubule Lattice Repair
Tuesday, April 09 \| 7:00PM - 8:00PM Social Hour with Lite Bites Room: Superior Lobby No registration fees are used to fund alcohol served at this function.
Tuesday, April 09 \| 7:30PM - 10:00PM Poster Session 2 Room: Superior
Wednesday, April 10 \| 7:00AM - 8:00AM Breakfast Room: Golden Cliff
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 1 of 9 * Erik M. Jorgensen, University of Utah, USA
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 2 of 9 Jan Ellenberg, European Molecular Biology Laboratory, Germany Analyzing Nuclear Pores and Chromosomes by Correlative Live, Super-Resolution and Electron Microscopy
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 3 of 9 Ralf Jungmann, Max Planck Institute of Biochemistry, Germany Super-Resolution Microscopy with DNA Molecules: Towards Localizomics
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 4 of 9 Colin Comerci, Stanford University, USA Short Talk: STED Microscopy Reveals Protein Self-Assembly Drives Localized Surface Layer Growth in C. crescentus
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Coffee Break
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 6 of 9 Elizabeth R. Wright, University of Wisconsin-Madison, USA Correlative Cryo-EM – Light Microscopy of Virus Entry/Fusion
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 7 of 9 Bram J. Koster, Leiden University Medical Center, Netherlands Zooming in on Cells and Molecules with Correlative Light and Electron Microscopy
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 8 of 9 Elizabeth A. Campbell, Rockefeller University, USA Short Talk: Cryo-EM Structures of an RNA Polymerase Promoter Melting Intermediate Elucidate DNA Unwinding
Wednesday, April 10 \| 8:00AM - 11:15AM Visualizing Cellular and Molecular Structures I Room: Ballroom 3 Speaker 9 of 9 Shrawan Kumar Mageswaran, California Institute of Technology, USA Short Talk: Electron Cryotomography of Mitochondrial Constriction Sites Urges Reevaluation of Mechanisms for Fission
Wednesday, April 10 \| 11:15AM - 5:00PM On Own for Lunch
Wednesday, April 10 \| 4:30PM - 5:00PM Coffee Available Room: Ballroom Lobby
Wednesday, April 10 \| 5:00PM - 7:00PM Visualizing Cellular and Molecular Structures II Room: Ballroom 3 Speaker 1 of 6 * Jan Ellenberg, European Molecular Biology Laboratory, Germany
Wednesday, April 10 \| 5:00PM - 7:00PM Visualizing Cellular and Molecular Structures II Room: Ballroom 3 Speaker 2 of 6 Martin Pilhofer, ETH Zurich, Switzerland Multiscale Models of Bacterial Cell-Cell Interactions
Wednesday, April 10 \| 5:00PM - 7:00PM Visualizing Cellular and Molecular Structures II Room: Ballroom 3 Speaker 3 of 6 Erik M. Jorgensen, University of Utah, USA Live Multicolor Super-Resolution Microscopy
Wednesday, April 10 \| 5:00PM - 7:00PM Visualizing Cellular and Molecular Structures II Room: Ballroom 3 Speaker 4 of 6 Xin Chen, Johns Hopkins University, USA Asymmetry from Symmetry or Symmetry from Asymmetry?
Wednesday, April 10 \| 5:00PM - 7:00PM Visualizing Cellular and Molecular Structures II Room: Ballroom 3 Speaker 5 of 6 Yuki Hamamura, University of Hamburg, Germany Short Talk: Analyzing the Dynamics of Meiotic Chromosomes Gives Mechanistic Insights into the Regulation of Meiotic Progression
Wednesday, April 10 \| 5:00PM - 7:00PM Visualizing Cellular and Molecular Structures II Room: Ballroom 3 Speaker 6 of 6 Collin Kieffer, University of Illinois at Urbana-Champaign, USA Short Talk: Moving Pathology into the 21st Century: 3D Multiscale Imaging of HIV in Tissues
Wednesday, April 10 \| 7:00PM - 7:15PM Meeting Wrap-Up: Outcomes and Future Directions (Organizers) Room: Ballroom 3
Wednesday, April 10 \| 7:15PM - 8:15PM Social Hour with Lite Bites Room: Ballroom 1 No registration fees are used to fund alcohol served at this function.
Wednesday, April 10 \| 8:00PM - 11:00PM Entertainment Room: Ballroom 1 Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.
Wednesday, April 10 \| 8:00PM - 11:00PM Cash Bar Room: Ballroom 1
Thursday, April 11 \| 10:24AM - 10:24AM Departure *Session Chair †Invited, not yet responded.