Web Desc
AAA+ Proteins: From Atomic Structures to Organisms
Organizer(s): Walid A. Houry, James Shorter, Antonina Roll-Mecak and Phyllis I. Hanson
Date: January 26 - 29, 2020
Location: Granlibakken Tahoe, Tahoe City, CA, USA
Supported by the Directors' Fund
Summary of Meeting:
AAA+ proteins are ATPases Associated with Diverse Cellular Activities. They form a large superfamily and have been found to carry out a very diverse range of functions including DNA replication, chromatin remodeling, rRNA processing, and membrane fusion. In recent years, there has been substantial progress in identifying the structure and cellular function of a large number of AAA+ proteins. However, little is known of the unique features underlying this mechano-chemical coupling mechanism used to apply forces to various substrates. There is also a lack of understanding for many AAA+ proteins regarding how substrates are recognized, regulation of their activities in vivo, and how their functions are coordinated with other competing and overlapping reactions. Recent findings demonstrate the importance of AAA+ proteins in the biology of cancer and neurodegeneration and as virulence factors in pathogenic microorganisms. However, we know very little of the biochemical pathways leading to diseases arising from mutations in these proteins. The conference will bring together an interdisciplinary group of scientists interested in understanding the structure, function, and disease aspects of these proteins. The conference comes at an opportune time of great developments in structural biology, especially cryoEM, and cell biology. We aim to provide a very interactive and collegial atmosphere to discuss the latest advances in this important field. In addition to having invited speakers, we will also provide the opportunity to many attendees to give an oral presentation about their research. Poster sessions will also be held for all attendees.
Scholarship Deadline: September 26 2019
Discounted Abstract Deadline: September 26 2019
Abstract Deadline: November 6 2019
Discounted Registration Deadline: November 21 2019
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

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Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

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Program

Sunday, January 26 | 4:00PM - 8:00PM
Arrival and Registration
Room: Pre Function


Sunday, January 26 | 6:00PM - 8:00PM
Welcome Mixer
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Sunday, January 26 | 6:00PM - 8:00PM
Light Meal - Provided by Granlibakken
Room: Granhall


Monday, January 27 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Monday, January 27 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 1 of 5
* Walid A. Houry, University of Toronto, Canada

Monday, January 27 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 2 of 5
* James Shorter, University of Pennsylvania, USA

Monday, January 27 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 3 of 5
* Antonina Roll-Mecak, DHHS/NINDS, National Institutes of Health, USA

Monday, January 27 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 4 of 5
* Phyllis I. Hanson, University of Michigan, USA

Monday, January 27 | 8:00AM - 9:00AM
Welcome and Keynote Address
Room: Mountain/Lake

Speaker 5 of 5
Bob T. Sauer, Massachusetts Institute of Technology, USA
Is ClpXP Unique or a Model for Other AAA+ Proteases and Protein Remodelers?

Monday, January 27 | 9:00AM - 11:30AM
Insights into AAA+ Mechanism of Function by CryoEM
Room: Mountain/Lake

Speaker 1 of 7
* Antonina Roll-Mecak, DHHS/NINDS, National Institutes of Health, USA

Monday, January 27 | 9:00AM - 11:30AM
Insights into AAA+ Mechanism of Function by CryoEM
Room: Mountain/Lake

Speaker 2 of 7
Daniel Southworth, University of California, San Francisco, USA
Structures and Mechanisms of Protein Unfolding Machines by Cryo-EM

Monday, January 27 | 9:00AM - 11:30AM
Insights into AAA+ Mechanism of Function by CryoEM
Room: Mountain/Lake


Coffee Break

Monday, January 27 | 9:00AM - 11:30AM
Insights into AAA+ Mechanism of Function by CryoEM
Room: Mountain/Lake

Speaker 4 of 7
Axel T. Brunger, Stanford University, USA
SNARE Complex Recycling and Quality Control by the AAA+ NSF ATPase

Monday, January 27 | 9:00AM - 11:30AM
Insights into AAA+ Mechanism of Function by CryoEM
Room: Mountain/Lake

Speaker 5 of 7
Andreas Martin, University of California, Berkeley, USA
Mechanisms of ATPase-Coupled Substrate Translocation by the 26S Proteasome and Related AAA+ Motors

Monday, January 27 | 9:00AM - 11:30AM
Insights into AAA+ Mechanism of Function by CryoEM
Room: Mountain/Lake

Speaker 6 of 7
Carol Soomin Cho, Korea Advanced Institute of Science and Technology, South Korea
Short Talk: Nucleotide-Dependent Structural Changes of the Abo1 AAA+ Histone Chaperone

Monday, January 27 | 9:00AM - 11:30AM
Insights into AAA+ Mechanism of Function by CryoEM
Room: Mountain/Lake

Speaker 7 of 7
Steven E. Glynn, Stony Brook University, USA
Short Talk: Structure of the AFG3L2 Mitochondrial AAA+ Protease Reveals the Molecular Basis for Activity in Health and Disease

Monday, January 27 | 11:30AM - 5:00PM
On Own for Lunch


Monday, January 27 | 11:30AM - 1:00PM
Poster Setup
Room: Bay


Monday, January 27 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 1 of 8
* Phyllis I. Hanson, University of Michigan, USA

Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 2 of 8
Kevin D. Corbett, University of California, San Diego, USA
Regulation of a Bacterial Innate-Immune Pathway by a TRIP13-Like ATPase

Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 3 of 8
Ryan R. Cupo, University of Pennsylvania, USA
Function of Skd3, a Mitochondrial AAA+ Protein

Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 4 of 8
Stephanie Gates, University of California, Berkeley, USA
VCP/p97 N-Terminal Domain Conformational Switching Regulates Cofactor Binding and Substrate Processing

Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 5 of 8
Irina Gutsche, Institut de Biologie Structurale, France
An Enterobacterial Stress Response Triad from a Cryo-EM Perspective

Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 6 of 8
Julia Kardon, Brandeis University, USA
Directed Unfolding by Mitochondrial ClpX Controls an Essential Biosynthetic Enzyme

Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 7 of 8
Saikrishnan Kayarat, Indian Institute of Science Education and Research, India
Mechanism of Activation of the AAA+ GTPase McrB by the Endonuclease McrC

Monday, January 27 | 2:30PM - 4:30PM
Workshop 1
Room: Mountain/Lake

Speaker 8 of 8
Teru Ogura, IMEG, Kumamoto University, Japan
Function of the p97-20S Proteasome and Nanodynamics of Novel and Canonical Proteasomal Pathway Components

Monday, January 27 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Monday, January 27 | 5:00PM - 7:00PM
AAA+ Proteins of the Cytoskeleton
Room: Mountain/Lake

Speaker 1 of 5
* James Shorter, University of Pennsylvania, USA

Monday, January 27 | 5:00PM - 7:00PM
AAA+ Proteins of the Cytoskeleton
Room: Mountain/Lake

Speaker 2 of 5
Antonina Roll-Mecak, DHHS/NINDS, National Institutes of Health, USA
Mechanism and Regulation of Microtubule Severing Enzymes

Monday, January 27 | 5:00PM - 7:00PM
AAA+ Proteins of the Cytoskeleton
Room: Mountain/Lake

Speaker 3 of 5
David W. Ehrhardt, Stanford University, USA
Mechanisms of Acentrosomal Microtubule Array Organization in Higher Plants

Monday, January 27 | 5:00PM - 7:00PM
AAA+ Proteins of the Cytoskeleton
Room: Mountain/Lake

Speaker 4 of 5
Arne Gennerich, Albert Einstein College of Medicine, USA
Short Talk: Force Generation of Mammalian Dynein-Dynactin is Augmented by Lis1

Monday, January 27 | 5:00PM - 7:00PM
AAA+ Proteins of the Cytoskeleton
Room: Mountain/Lake

Speaker 5 of 5
Chun-che Tseng, Mayo Clinic Graduate School of Biomedical Sciences, USA
Short Talk: Conserved Regulation of AAA-ATPase Vps4 by V Domain of Bro1 Family Proteins Contributes to ESCRT-III Function

Monday, January 27 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Monday, January 27 | 7:00PM - 8:00PM
Social Hour
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Monday, January 27 | 7:30PM - 10:00PM
Poster Session 1
Room: Bay


Tuesday, January 28 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake

Speaker 1 of 8
* Teru Ogura, IMEG, Kumamoto University, Japan

Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake

Speaker 2 of 8
Tarun M. Kapoor, Rockefeller University, USA
Chemical Probes for AAA+ Proteins

Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake

Speaker 3 of 8
Conrad C. Weihl, Washington University School of Medicine, USA
Multisystem Proteinopathy Mutations Define New Functions for p97/VCP

Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake


Coffee Break

Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake

Speaker 5 of 8
James H. Hurley, University of California, Berkeley, USA
Roles of VPS4 and Spastin in ESCRT-III Membrane Scission and Disassembly

Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake

Speaker 6 of 8
Xiaodong Zhang, Imperial College London, UK
Mechanistic Insights into Non-Translocating AAA ATPases: From INO80 Chromatin Remodeller to Transcription Activators

Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake

Speaker 7 of 8
Aaron L. Lucius, University of Alabama at Birmingham, USA
Short Talk: Falling Off: ClpB and Hsp104 Operate as Non-Processive Translocases

Tuesday, January 28 | 8:00AM - 11:00AM
Role of AAA+ Proteins in Cellular Remodeling
Room: Mountain/Lake

Speaker 8 of 8
Zev A. Ripstein, Hospital for Sick Children, Canada
Short Talk: A Processive Rotary Mechanism Couples Substrate Unfolding and Proteolysis in the ClpXP Degradation Machinery

Tuesday, January 28 | 11:00AM - 5:00PM
On Own for Lunch


Tuesday, January 28 | 11:00AM - 1:00PM
Poster Setup
Room: Bay


Tuesday, January 28 | 1:00PM - 10:00PM
Poster Viewing
Room: Bay


Tuesday, January 28 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Tuesday, January 28 | 5:00PM - 7:00PM
Role of AAA+ Proteins in Regulating DNA and RNA Complexes
Room: Mountain/Lake

Speaker 1 of 6
* Andreas Martin, University of California, Berkeley, USA

Tuesday, January 28 | 5:00PM - 7:00PM
Role of AAA+ Proteins in Regulating DNA and RNA Complexes
Room: Mountain/Lake

Speaker 2 of 6
Robin E. Stanley, NIEHS, National Institutes of Health, USA
Role of AAA-ATPases in Ribosome Assembly

Tuesday, January 28 | 5:00PM - 7:00PM
Role of AAA+ Proteins in Regulating DNA and RNA Complexes
Room: Mountain/Lake

Speaker 3 of 6
James M. Berger, Johns Hopkins University School of Medicine, USA
Conformational Coupling and Substrate-Recognition Plasticity in Replicative AAA+ ATPases

Tuesday, January 28 | 5:00PM - 7:00PM
Role of AAA+ Proteins in Regulating DNA and RNA Complexes
Room: Mountain/Lake

Speaker 4 of 6
Matt J. Jaremko, Cold Spring Harbor Laboratory, USA
Short Talk: The Dynamic Conformations of the Human Origin Recognition Complex

Tuesday, January 28 | 5:00PM - 7:00PM
Role of AAA+ Proteins in Regulating DNA and RNA Complexes
Room: Mountain/Lake

Speaker 5 of 6
Helmut Bergler, Karl-Franzens University Graz, Austria
Short Talk: Mechanism of Chemical Inhibition of the Essential Ribosome Assembly

Tuesday, January 28 | 5:00PM - 7:00PM
Role of AAA+ Proteins in Regulating DNA and RNA Complexes
Room: Mountain/Lake

Speaker 6 of 6
Andrew Frank Neuwald, University of Maryland School of Medicine, USA
Short Talk: Deep Analysis of Residue Constraints (DARC): Identifying Determinants of AAA+ ATPase Functional Specificity

Tuesday, January 28 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Tuesday, January 28 | 7:00PM - 8:00PM
Social Hour
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Tuesday, January 28 | 7:30PM - 10:00PM
Poster Session 2
Room: Bay


Wednesday, January 29 | 7:00AM - 8:00AM
Breakfast
Room: Granhall


Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 1 of 9
* Arne Gennerich, Albert Einstein College of Medicine, USA

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 2 of 9
Walid A. Houry, University of Toronto, Canada
Role of the ClpXP Protease in Pathogenesis

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 3 of 9
Rose E. Goodchild, VIB/ KU Leuven, Belgium
How Torsins Regulate Cellular Lipid Metabolism

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake


Coffee Break

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 5 of 9
Christopher P. Hill, University of Utah, USA
Translocation Mechanism of AAA Unfoldases

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 6 of 9
Tania A. Baker, Massachusetts Institute of Technology, USA
Are Two Rings Better than One? Deciphering Roles of the ATPase Modules in the Double Ring AAA+ Unfoldase ClpA

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 7 of 9
Daisuke Morito, Showa University, Japan
Short Talk: The 591 kDa AAA+/Ubiquitin Ligase Regulates the Cellular Fat Metabolism

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 8 of 9
Nina Marie Wolf, University of Illinois, Chicago, USA
Short Talk: Structures of ClpC1 from Mycobacterium Tuberculosis in Complex with Macrocyclic Inhibitors from Natural Products Reveal Critical Binding Interactions

Wednesday, January 29 | 8:00AM - 11:15AM
AAA+ Proteins in Disease Pathways
Room: Mountain/Lake

Speaker 9 of 9
Minglei Zhao, University of Chicago, USA
Short Talk: Mechanistic Insights into p97 Inhibition through Disulfiram Derivative

Wednesday, January 29 | 11:15AM - 5:00PM
On Own for Lunch


Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 1 of 8
* Xiaodong Zhang, Imperial College London, UK

Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 2 of 8
Kyle E. Lopez, University of California, San Francisco, USA
Conformational Plasticity of the ClpAP AAA+ Protease Couples Protein Unfolding and Proteolysis

Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 3 of 8
Ying Lu, Harvard Medical School, USA
An Empirical Free-Energy Landscape Reveals the Global Conformational Dynamics of Proteasomal ATPases

Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 4 of 8
Wen Ma, University of California, San Diego, USA
Molecular Mechanism of Polypeptide Translocation by an AAA+ Unfoldase Studied by Long Timescale Atomistic Simulations

Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 5 of 8
Oliver Mueller-Cajar, Nanyang Technological University, Singapore
Insights into the Mechanism and Regulation of the CbbQO-type Rubisco Activase, a MoxR AAA+ ATPase

Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 6 of 8
Thomas U. Schwartz, Massachusetts Institute of Technology, USA
On the Unusual Assembly States of the AAA+ ATPase TorsinA

Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 7 of 8
Mia Shin, The Scripps Research Institute, USA
Structural Basis for Distinct Operational Modes and Protease Activation in Lon Protease

Wednesday, January 29 | 2:30PM - 4:30PM
Workshop 2
Room: Mountain/Lake

Speaker 8 of 8
David Smith, West Virginia University School of Medicine, USA
Functional and Regulatory Roles Played by the N-Domain of the Proteasomal ATPase PAN

Wednesday, January 29 | 4:30PM - 5:00PM
Coffee Available
Room: Pre Function


Wednesday, January 29 | 5:00PM - 6:45PM
AAA+ Proteins and Therapeutics
Room: Mountain/Lake

Speaker 1 of 2
James Shorter, University of Pennsylvania, USA
AAA+ Disaggregases as Therapeutic Agents

Wednesday, January 29 | 5:00PM - 6:45PM
AAA+ Proteins and Therapeutics
Room: Mountain/Lake

Speaker 2 of 2
Phyllis I. Hanson, University of Michigan, USA
Role of AAA+ Protein in Late Endosomes

Wednesday, January 29 | 6:45PM - 7:00PM
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Room: Mountain/Lake


Wednesday, January 29 | 7:00PM - 8:00PM
Dinner
Room: Granhall


Wednesday, January 29 | 7:00PM - 8:00PM
Social Hour
Room: Granhall
No registration fees are used to fund alcohol served at this function.

Wednesday, January 29 | 8:00PM - 11:00PM
Entertainment
Room: Granhall
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Thursday, January 30 | 10:24AM - 10:24AM
Departure


*Session Chair.