Web Desc
Proteomics in Cell Biology and Disease - VIRTUAL CONTENT AVAILABLE ON DEMAND
Organizer(s): Jennie R. Lill, Forest M. White and Kathryn S. Lilley
Date: September 21 - 23, 2020
Location: Virtual at your computer. Recorded presentation content is available On Demand.
Part of the Keystone Symposia Global Health Series, supported by the Bill & Melinda Gates Foundation
For important information on the coronavirus, please click here
Summary of Meeting:
Proteomics provides critical biological information about the activation state of enzymes, pathways, and signaling networks that drive all biological processes. However, the power of this approach is currently limited by the capabilities of proteomics technologies and methods. Although single dimensional proteomics analysis is now routine on abundant samples, challenges remain in analyzing limited samples collected from in vivo studies and in examining dynamic cellular processes. This meeting aims to address these challenges, focusing on the latest advances and innovations in sample preparation, instrumentation, and bioinformatic/computational analysis, to push the boundaries of proteomics to enable more powerful analytics for translational research. Specifically, the program will cover: (1) cutting edge sample preparation methods that enable increased sensitivity and functional analysis of proteins; (2) approaches to tackle single cell proteomics analysis, in parallel with genomics and transcriptomics advances; (3) advancing multiplex capabilities beyond 2-10 plex experimentation to for more meaningful clinical analysis; and (4) integration of various “omics” data sets with proteomics to provide a more global view of the dynamic cellular environment. A critical focus of this meeting will be on new technologies needed to further improve the feasibility of looking at multicellular dimensions & multiplexed analyses in proteomics. The program features diverse content on current biological applications and needs, highlighting the application of high sensitivity proteomics to real-world translational questions. Attendees will learn what instrumentation, data acquisition methods, and informatic pipelines allow researchers to answer critical yet challenging questions, while also learning what is not yet feasible. By bringing together a multidisciplinary team of experts across a broad range of fields, this meeting aims to stimulate a collaborative environment and cross-pollination of ideas to drive the next wave of innovation in proteomics approaches and technologies. We will also discuss how we can better work together as a community to disseminate these methods and analytical tools, similar to recent movements in the open source computational programming world. Ultimately our goal is to help build a proteomics community that is ready to tackle even the most complex biological problems.
Global Health Travel Award Deadline: August 24 2020
Scholarship Deadline: August 24 2020
Abstract Deadline: September 3 2020
We gratefully acknowledge additional support for this conference from:
ReSyn Biosciences
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Monday, September 21 | 8:00AM - 8:10AM
Welcoming Remarks (8am Denver/Mountain Time Start)


Monday, September 21 | 8:10AM - 8:40AM
Keynote Address (8:10am Denver/Mountain Time Start)

Speaker 1 of 2
* Jennie R. Lill, Genentech, Inc., USA

Monday, September 21 | 8:10AM - 8:40AM
Keynote Address (8:10am Denver/Mountain Time Start)

Speaker 2 of 2
Alice Y. Ting, Stanford University, USA
Proximity Labeling for Mapping Spatial Proteomes and Transcriptomes in Living Cells

Monday, September 21 | 8:40AM - 10:35AM
Studying the Cell Surfaceome (8:40am Denver/Mountain Time St
art)


Speaker 1 of 6
* Nadia Martinez-Martin, Genentech, Inc., USA
Mapping the Human Immunoglobulin Superfamily Receptome to Identify Novel Cancer-Relevant Pathways

Monday, September 21 | 8:40AM - 10:35AM
Studying the Cell Surfaceome (8:40am Denver/Mountain Time St
art)


Speaker 2 of 6
Bernd Wollscheid, ETH Zürich, Switzerland
Light-Mediated Discovery of Surfaceome Nanoscale Organization and Inter-Cellular Receptor Interaction Networks

Monday, September 21 | 8:40AM - 10:35AM
Studying the Cell Surfaceome (8:40am Denver/Mountain Time St
art)



Coffee Break

Monday, September 21 | 8:40AM - 10:35AM
Studying the Cell Surfaceome (8:40am Denver/Mountain Time St
art)


Speaker 4 of 6
* Gavin J. Wright, Wellcome Trust Sanger Institute, UK
Large-Scale Systematic Approaches to Identify Novel Receptor-Ligand Pairs that Initiate Intercellular Signaling

Monday, September 21 | 8:40AM - 10:35AM
Studying the Cell Surfaceome (8:40am Denver/Mountain Time St
art)


Speaker 5 of 6
Sofia Massaro Tieze, Yale University, USA
Short Talk: Identification of Palmitoyl Protein Thioesterase Substrates Defines Roles for Synaptic Depalmitoylation

Monday, September 21 | 8:40AM - 10:35AM
Studying the Cell Surfaceome (8:40am Denver/Mountain Time St
art)


Speaker 6 of 6
Monika Vishnoi, Houston Methodist Research Institute, USA
Short Talk: Matrisome Signatures in GBM Heterogeneity

Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 1 of 8
* Kathryn S. Lilley, University of Cambridge, UK

Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 2 of 8
Emma Borgeson, University of Gothenburg, Sweden
Advanced Multiplex Protein Analysis of Obesity-Related Cardiometabolic Biomarkers in Human Plasma and Serum

Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 3 of 8
Dustin T. King, Simon Fraser University, Canada
Ultraviolet Photodissociation Mass Spectrometry
Enables Precise Mapping of O-GlcNAc Sites in Proteins


Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 4 of 8
Josie Amber Christopher, University of Cambridge, UK
Spatial Dynamics of the DNA Damage Proteome

Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 5 of 8
Daniel Hornburg, Seer, USA
Proteograph™, a Multi-Nanoparticle Platform, Enables Plasma Proteomics Profiling at Scale and Speed, Significantly Improving Coverage and Scalability versus Traditional Deep fractionation methods

Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 6 of 8
Justin McKetney, University of Wisconsin-Madison, USA
Proteomic Analysis of Cerebrospinal Fluid in Alzheimer’s Disease

Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 7 of 8
Nicolas Hartel, University of Southern California, USA
Deep Protein Arginine Methylation Profiling by LC-MS Proteomics

Monday, September 21 | 10:35AM - 11:00AM
Lightning Talks (10:35am Denver/Mountain Time Start)

Speaker 8 of 8
Samuel B. Pollock, Genentech, Inc., USA
Semi-Automated MHC-I Enrichment and TOMAHAQ Mass Spectrometry for the Sensitive and Quantitative Detection of Neoepitopes

Monday, September 21 | 12:00PM - 2:00PM
Poster Session (12pm Denver/Mountain Time Start)


Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 1 of 9
Jyoti Choudhary, Institute of Cancer Research, UK
Integrative Proteogenomics Deconvoluting the Landscape of Lung Adenocarcinoma in Never-Smokers

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 2 of 9
* Lennart Martens, Ghent University, Belgium
Machine Learning Powered Next-Generation Proteomics

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 3 of 9
* Olga Vitek, Northeastern University, USA
Challenges in Large-Scale Computational Mass Spectrometry and Multiomics

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)



Coffee Break

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 5 of 9
Mikhail Savitski, European Molecular Biology Laboratory, Germany
Phenotyping Cellular States with Functional Proteomics

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 6 of 9
Kevin Drew, University of Texas at Austin, USA
Short Talk: Hu.MAP2.0: Integration of over 15,000 Proteomic Experiments Builds a Global Compendium of Human Multiprotein Assemblies

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 7 of 9
Nader Morshed, Massachusetts Institute of Technology, USA
Short Talk: Quantitative Phosphoproteomics Uncovers Dysregulated Kinase Networks in Alzheimer’s Disease

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 8 of 9
Devin K. Schweppe, University of Washington, USA
Short Talk: Millisecond Informatics: Real-Time Analytics for Quantitative Proteomics

Monday, September 21 | 2:00PM - 5:00PM
Dissection of Complex Data Sets: Informatic and Statistical
Tools of the Trade (2pm Denver/Mountain Time Start)


Speaker 9 of 9
Nicholas M. Riley, Stanford University, USA
Short Talk: Designing Methods and Software to Suit the needs of O-glycopeptide Characterization

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)


Speaker 1 of 8
* Jennie R. Lill, Genentech, Inc., USA

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)


Speaker 2 of 8
* Matthew S. Bogyo, Stanford University School of Medicine, USA
Chemical Proteomic Tools for Identification and Imaging of Enzymes Involved in Cancer and Infectious Disease

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)


Speaker 3 of 8
Edward M. Marcotte, University of Texas at Austin, USA
Single Molecule Protein Sequencing

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)


Speaker 4 of 8
Ryan Kelly, Brigham Young University, USA
One Cell at a Time: Nanodroplet Sample Preparation Enables In-Depth Single Cell Proteomics

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)



Coffee Break

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)


Speaker 6 of 8
Joseph Gogain, SomaLogic, Inc, USA
Discovery and Validation of a Blood-Based Protein Signatures for Assessing Human Health

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)


Speaker 7 of 8
Laura Muehlbauer, University of Wisconsin-Madison, USA
Short Talk: Global Phosphoproteome Analysis using FAIMS on a Hybrid Orbitrap Mass Spectrometer

Tuesday, September 22 | 8:00AM - 11:00AM
Working Towards Single Cell Proteomics (8am Denver/Mountain
Time Start)


Speaker 8 of 8
Mahmoud-reza Rafiee, Francis Crick Institute, UK
Short Talk: SPACE Exploration of RNA-Binding Proteins Reveals Diminished Chromatin-Binding of Mutant VCP in a Human Stem Cell Model of Neurodegeneration

Tuesday, September 22 | 12:00PM - 2:00PM
Poster Session (12pm Denver/Mountain Time Start)


Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 1 of 9
* Nathalie Agar, Brigham and Womens Hospital, USA
Mass Spectrometry Imaging Methodologies, to Study Neuro-Oncology in Brain Cancer

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 2 of 9
Jonathan S. Weissman, Whitehead Institute and MIT, USA
Multidimensional Omics - Harnessing Ribosomal Profiling and Proteomics to Investigate Non Canonical Translation Events in Disease

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 3 of 9
Claudia Andrea Escher, Biognosys AG, Switzerland
Proteomics for Precision Medicine: A High-Throughput Platform for Proteome and Phospho-Proteome

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)



Coffee Break

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 5 of 9
Aditya Murthy, Genentech, Inc., USA
Short Talk: Multiplexed Proteomics in Macrophages Uncovers Novel Roles of Autophagy in Anti-Microbial Immunity

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 6 of 9
Roberta Migale, Francis Crick Institute, UK
Short Talk: Dynamics of FOXL2 Gene Regulation in the Mouse Ovary

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 7 of 9
Liang Zhang, City University of Hong Kong, Hong Kong
Short Talk: Dissect RNA-Protein Interactome in Living Cells Using CRISPR-Assisted Proximity Labeling

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 8 of 9
Carlos Cruchaga, Washington University School of Medicine, USA
Short Talk: Genomic and Multi-Tissue Proteomic Integration for Understanding the Biology of Disease and other Complex Traits

Tuesday, September 22 | 2:00PM - 5:00PM
Integrative Multidimensional Omics (2pm Denver/Mountain Time
Start)


Speaker 9 of 9
* Christine Vogel, New York University, USA
Short Talk: Integrative Systems Profiling Identifies New Signatures of Neurodegeneration – and Novel Roles of the Core Proteasome

Wednesday, September 23 | 8:00AM - 11:00AM
Spatial Proteomics (8am Denver/Mountain Time Start)

Speaker 1 of 7
* Kathryn S. Lilley, University of Cambridge, UK
Spatial Organization of the Transcriptome and Proteome

Wednesday, September 23 | 8:00AM - 11:00AM
Spatial Proteomics (8am Denver/Mountain Time Start)

Speaker 2 of 7
Anne-Claude Gingras, Lunenfeld-Tanenbaum Research Institute, Canada
Tracking Spatiotemporal Proteomics in Complex Systems using Proximity Tagging

Wednesday, September 23 | 8:00AM - 11:00AM
Spatial Proteomics (8am Denver/Mountain Time Start)

Speaker 3 of 7
* Matthias Trost, Newcastle University, UK
Proteome Analysis Identifies Novel Roles for Ubiquitylation in the Innate Immune Response from the Phagosome

Wednesday, September 23 | 8:00AM - 11:00AM
Spatial Proteomics (8am Denver/Mountain Time Start)


Coffee Break

Wednesday, September 23 | 8:00AM - 11:00AM
Spatial Proteomics (8am Denver/Mountain Time Start)

Speaker 5 of 7
Cecilia Lindskog, Uppsala University, Sweden
The Human Protein Atlas – Integrated Omics for Single Cell Analysis

Wednesday, September 23 | 8:00AM - 11:00AM
Spatial Proteomics (8am Denver/Mountain Time Start)

Speaker 6 of 7
Xinying Zong, St. Jude Children's Research Hospital, USA
Short Talk: Dynamic cis-Proteomics Comprehensively Reveals Signal-Dependent Regulators

Wednesday, September 23 | 8:00AM - 11:00AM
Spatial Proteomics (8am Denver/Mountain Time Start)

Speaker 7 of 7
Ashley Frankenfield, George Washington University, USA
Short Talk: A Specific and Sensitive Proximity-labeling Proteomics Approach for Studying Protein-Protein Interactions of the Lysosome Membrane

Wednesday, September 23 | 12:30PM - 1:30PM
Career Roundtable (12:30pm Denver/Mountain Time Start)

Speaker 1 of 3
Anne-Claude Gingras, Lunenfeld-Tanenbaum Research Institute, Canada

Wednesday, September 23 | 12:30PM - 1:30PM
Career Roundtable (12:30pm Denver/Mountain Time Start)

Speaker 2 of 3
Marcus Bantscheff, GlaxoSmithKline, Germany

Wednesday, September 23 | 12:30PM - 1:30PM
Career Roundtable (12:30pm Denver/Mountain Time Start)

Speaker 3 of 3
Nathalie Agar, Brigham and Womens Hospital, USA

Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)


Speaker 1 of 8
* Forest M. White, Massachusetts Institute of Technology, USA
Phosphoproteomics for Characterization of Therapeutic Resistance Pathways

Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)


Speaker 2 of 8
Jennie R. Lill, Genentech, Inc., USA
Hippo Pathway Deregulation in Cancer

Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)


Speaker 3 of 8
David E. James, University of Sydney, Australia
Personalised Phosphoproteomics – Studying the Exercise Response in Individuals

Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)



Coffee Break

Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)


Speaker 5 of 8
* Marcus Bantscheff, GlaxoSmithKline, Germany
Chemoproteomics for the Elucidation of Signaling Networks

Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)


Speaker 6 of 8
Xiaoyu Zhang, The Scripps Research Institute, USA
Short Talk: Electrophilic PROTACs that Degrade Nuclear Proteins by Engaging DCAF16

Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)


Speaker 7 of 8
Nida Haider, Joslin Diabetes Center/ Harvard Medical School, USA
Short Talk: Distinct Male and Female Phosphoproteomic Signatures Underlie
Human Insulin Resistance


Wednesday, September 23 | 2:00PM - 4:45PM
Elucidation of in vivo Signaling Networks (2pm Denver/Mounta
in Time Start)


Speaker 8 of 8
Brendan Floyd, University of Texas at Austin, USA
Short Talk: Systematic Identification of Protein Phosphorylation-Mediated Interactions

Wednesday, September 23 | 4:45PM - 5:00PM
Closing Remarks (4:45pm Denver/Mountain Time Start)


*Session Chair.