Web Desc
Precision Engineering of the Genome, Epigenome and Transcriptome - VIRTUAL CONTENT AVAILABLE ON DEMAND
joint with Plant Genome Engineering: From Lab to Field - VIRTUAL CONTENT AVAILABLE ON DEMAND
Organizer(s): Charles Gersbach, Rachel E. Haurwitz and Fyodor D. Urnov
Date: March 08 - 10, 2021
Location: Virtual at your computer. Recorded presentation content is available On Demand.
Sponsored by BlueRock Therapeutics, Novo Nordisk A/S, Synthego, TCR² Therapeutics and Vertex Pharmaceuticals Incorporated.
For important information on the coronavirus, please click here
Summary of Meeting:
Over the last several years, new genome engineering tools have revolutionized the ability to interrogate biological systems, engineer innovative biotechnologies, and model and treat human disease. The genome engineering field continues to evolve at a frenzied pace, including both the development of new fundamental tools and their application to addressing societal needs. This Keystone Symposia conference will cover this spectrum of advances, including the most recent technological innovations in genome, epigenome, and transcriptome precision engineering. The conference will also highlight recent advances in developing human therapeutics with these tools, including emerging clinical trial results and preclinical examples of novel modes of intervention. The conference will include a variety of topics of interest to the broad research community, including next-generation applications such as high-throughput screening, synthetic biology, and chromatin and RNA imaging and diagnostics. Finally, the conference will conclude with examples of how emerging technologies can be integrated for an unprecedented level of resolution of cellular processes, such as genome engineering combined with single-cell analysis methods. Collectively, this meeting will build on its history of success by familiarizing the audience with the latest advances in tool development, providing an update on clinical application of these tools, and generating an appreciation for the breadth of scientific areas impacted by the Genome Engineering Revolution.
Scholarship Deadline: February 22 2021
Abstract Deadline: February 9 2021
Keystone Symposia thanks our Sponsor(s) for generously supporting this meeting:
BlueRock TherapeuticsNovo Nordisk A/SSynthegoTCR² TherapeuticsVertex Pharmaceuticals Incorporated
We gratefully acknowledge additional support from these exhibitors at this conference:
Intellia Therapeutics
We gratefully acknowledge additional support for this conference from:
DCVC BioHorizon DiscoveryResolve BioSciences GmbH
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Monday, March 08 | 8:00AM - 8:40AM
Welcome and Keynote Address (8am Denver/Mountain Time Start)

Speaker 1 of 2
* Fyodor D. Urnov, University of California, Berkeley, USA

Monday, March 08 | 8:00AM - 8:40AM
Welcome and Keynote Address (8am Denver/Mountain Time Start)

Speaker 2 of 2
Tim W. Yu, Boston Children's Hospital, USA
Developing Custom Genetic Therapies

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)


Speaker 1 of 7
* Samuel H. Sternberg, Columbia University, USA
Targeted DNA Integration using CRISPR RNA-Guided Transposases

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)


Speaker 2 of 7
Cecilia Cotta-Ramusino, Tessera Therapeutics, USA
[NOT AVAILABLE ON DEMAND] New Editing Technologies

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)



Coffee Break

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)


Speaker 4 of 7
* Patrick D. Hsu, University of California, Berkeley, USA
CRISPR in the Time of COVID

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)


Speaker 5 of 7
Prashant Mali, University of California, San Diego, USA
[NOT AVAILABLE ON DEMAND] Endogenous RNA Editing Systems

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)


Speaker 6 of 7
Balint Csorgo, UCSF, USA
Short Talk: A Compact Cascade-Cas3 System for Targeted Genome Engineering and Gene Discovery

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)


Speaker 7 of 7
Yarui Diao, Duke University School of Medicine, USA
Short Talk: CRISPR-based Multimodal Functional Analysis Reveals the Key Role of Retroviral Sequence LTR7 in Governing Neural Fate Commitment of Human Embryo Development

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 1 of 7
* Daniel F. Voytas, University of Minnesota, USA
Overcoming Bottlenecks in Editing Plant Genomes


Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 2 of 7
Feng Zhang, University of Minnesota, USA
Improving Scalable and Precision Genome Editing in Plants


Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)
This session is from Plant Genome Engineering



Coffee Break


Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 4 of 7
* Holger Puchta, Karlsruhe Institute of Technology, Germany
CRISPR/Cas Mediated Plant Chromosome Engineering


Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 5 of 7
Avraham A. Levy, Weizmann Institute of Science, Israel
From a DNA Break to Targeted Recombination between Homologous Chromosomes in Plants


Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 6 of 7
Ayumu Takatsuka, Tohoku University, Japan
Short Talk: MitoTALEN-Mediated Mitochondrial Gene-Knockout Revealed a Cytoplasmic Male Sterility-Causative Gene in Oryza Sativa cv. Tadukan


Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 7 of 7
Shin-ichi Arimura, University of Tokyo, Japan
Short Talk: Targeted Base Editing in the Plastid Genome of Arabidopsis thaliana


Monday, March 08 | 12:30PM - 1:30PM
Career Roundtable (Joint) (12:30pm Denver/Mountain Time Star
t)

Speaker 1 of 3
Britt S. Adamson, Princeton University, USA

Monday, March 08 | 12:30PM - 1:30PM
Career Roundtable (Joint) (12:30pm Denver/Mountain Time Star
t)

Speaker 2 of 3
Rachel E. Haurwitz, Caribou Biosciences, Inc., USA

Monday, March 08 | 12:30PM - 1:30PM
Career Roundtable (Joint) (12:30pm Denver/Mountain Time Star
t)

Speaker 3 of 3
Steven E. Jacobsen, University of California, Los Angeles, USA

Monday, March 08 | 1:30PM - 3:00PM
Poster Session 1 (Joint) (1:30pm Denver/Mountain Time Start)


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 1 of 10
* Charles Gersbach, Duke University, USA

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 2 of 10
Jacob E. Corn, ETH Zürich, Switzerland
Visible and Invisible HDR in Human Cells

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 3 of 10
Bas van Steensel, Netherlands Cancer Institute, Netherlands
Multiplexed Probing of the Impact of Chromatin on Cas9-Induced DSB Repair Pathways

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 4 of 10
Ahmad S. Khalil, Boston University, USA
Design of Eukaryotic Gene Regulatory Circuits

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)


Coffee Break

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 6 of 10
Jennifer E. Phillips-Cremins, University of Pennsylvania, USA
Engineering the Repetitive 3D Genome in Human Disease

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 7 of 10
Alyna Katti, Weill Cornell Medicine, USA
Short Talk: Strategies to Improve Engineering Cancer Associated SNVs with Base Editing

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 8 of 10
Daniel M. Sapozhnikov, McGill University, Canada
Short Talk: Unraveling the Transcriptional Impact of DNA Demethylation at Specific Promoters by Targeted Steric Blockage of DNA Methyltransferase with CRISPR/dCas9

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 9 of 10
* Henriette O'Geen, University of California, Davis, USA
Short Talk: Determinants of Persistence in Epigenetic Editing

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)

Speaker 10 of 10
Isaac B. Hilton, Rice University, USA
Short Talk: Programmable Human Histone Phosphorylation and Gene Activation using a CRISPR/Cas9-Based Chromatin Kinase

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 1 of 8
* Steven E. Jacobsen, University of California, Los Angeles, USA
Epigenetic Editing in Plants


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 2 of 8
Seiichi Toki, National Agriculture and Food Research Organization, Japan
Plant DNA Repair and Genome Engineering


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 3 of 8
Erika Toda, Tokyo Metropolitan University, Japan
An Efficient DNA- and Selectable-Marker-Free Genome-Editing System Using Zygotes in Rice and Its Possible Application to Other Crop Species


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering



Coffee Break


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 5 of 8
* Caixia Gao, Chinese Academy of Sciences, China
Precise Prime Editing in Plants


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 6 of 8
Lanqin Xia, Chinese Academy of Agricultural Sciences, China
CRISPR/Cas-Mediated Precision Genome Editing for Crop Improvement


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 7 of 8
Jian-Kang Zhu, Shanghai Center for Plant Stress Biology, CAS, China
Strategies for Efficient Gene Targeting in Plants


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 8 of 8
Keishi Osakabe, Tokushima University, Japan
Short Talk: Genome Editing Using the CRISPR Type I-D Nuclease in Plants


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 1 of 9
* Rachel E. Haurwitz, Caribou Biosciences, Inc., USA
The Future is Allogeneic - Translating a CRISPR Platform into Gene-Edited Cell Therapies

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 2 of 9
Matthew Porteus, Stanford University School of Medicine, USA
Genome Editing of Blood Cells to Cure Disease

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 3 of 9
Chiara Bonini, Vita-Salute San Raffaele University, Italy
T-Cell Gene Editing for Cancer Immunotherapy

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)



Coffee Break

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 5 of 9
* Nicole Gaudelli, Beam Therapeutics, USA
An Adenine Base Editing Strategy for the Treatment of Sickle Cell Disease by Elimination of the Pathogenic Globin Protein

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 6 of 9
Delphine Guipouy, CHU Sainte Justine Research Center, Canada
[NOT AVAILABLE ON DEMAND] Short Talk: Knockout of NKG2A by CRISPR in Human NK Cells Enhance their Cytotoxic Activity Against Solid Tumors

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 7 of 9
Dimitrios Laurin Wagner, Charité - Universitätsmedizin Berlin, Germany
Short Talk: Fast and Efficient Generation of TRAC-integrated CAR T Cells without Viruses

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 8 of 9
Adrian Veres, Harvard University, USA
Short Talk: Optimizing Stem Cell Lines for in vitro-derived Beta Cell Therapeutics via Genome-wide CRISPR Knock-out Screening

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)


Speaker 9 of 9
Sébastien Levesque, CHU de Québec - Université Laval, Canada
Short Talk: Expanding the Scope of Marker-Free Selection for CRISPR-Driven Genome Editing in Human Cells

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 1 of 9
* Zachary Lippman, Howard Hughes Medical Institute/Cold Spring Harbor Laboratory, USA
Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 2 of 9
* Jens Boch, Leibniz Universität Hannover, Germany
Expanding the TALEN Toolbox for Genome Editing


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 3 of 9
Joyce Van Eck, Boyce Thompson Institute, USA
Exploring Insect Interactions in Physalis Using CRISPR Generated Mutant Lines


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering



Coffee Break


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 5 of 9
Magdy Mahfouz, King Abdullah University of Science and Technology, Saudi Arabia
RNA-Virus Interference via CRISPR-Cas13 Systems


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 6 of 9
Mariette Andersson, Swedish University of Agricultural Sciences, Sweden
Genome Editing for Improved Potato Traits


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 7 of 9
Eyal Fridman, Volcani Center ARO, Israel
Short Talk: RECAS9-Mediated QTL Mapping by Mitotic Genome Editing in Cereals


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 8 of 9
Sergei Svitashev, Corteva Agriscience, USA
Short Talk: CRISPR-Cas9-mediated 75.5 Mb Inversion in Maize


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)
This session is from Plant Genome Engineering


Speaker 9 of 9
Trevor Weiss, University of Minnesota, USA
Short Talk: Differential CRISPR/Cas9 Genome Editing Influenced by Epigenetic Factors


Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 1 of 6
Roland George Roberts, Public Library of Science, UK

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 2 of 6
Holger Matthias Breithaupt, EMBO, Germany

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 3 of 6
Kevin Davies, CRISPR Journal, USA

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 4 of 6
Bing Yang, Frontiers in Genome Editing, USA

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 5 of 6
Ruth Zearfoss, Cell Press, USA

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 6 of 6
Caitlin Karniski, Nature Portfolio, USA

Tuesday, March 09 | 1:30PM - 3:00PM
Poster Session 2 (Joint) (1:30pm Denver/Mountain Time Start)


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)


Speaker 1 of 8
David G. Ousterout, Locus Biosciences, Inc., USA
Development of crPhage to Treat Infections

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)


Speaker 2 of 8
* Fyodor D. Urnov, University of California, Berkeley, USA
Towards a CRISPRi/a Therapeutic for Radiation Injury

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)


Speaker 3 of 8
Aravind Asokan, Duke University, USA
Engineering AAV for Targeted In Vivo Editing

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)



Coffee Break

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)


Speaker 5 of 8
* Ana Moreno Collado, Navega Therapeutics, USA
Epigenome Regulation for Treatment of Chronic Pain

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)


Speaker 6 of 8
Chikdu Shivalila, Wave Life Sciences, USA
Short Talk: A Versatile Platform for ADAR-Mediated RNA Editing in vivo in Preclinical Models

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)


Speaker 7 of 8
Jia Qi Cheng-Zhang, University of Pittsburgh, USA
Short Talk: Upregulation of Disease Compensatory Gene via CRISPR Activation in Muscular Dystrophy Mice

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)


Speaker 8 of 8
Serena Tamura, University of California San Francisco, USA
Short Talk: CRISPR Activation to Rescue SCN2A Haploinsufficiency in Autism Spectrum Disorder

Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 1 of 7
* Kan Wang, Iowa State University, USA
Breeding of a Transformable Model Maize Line for Genome Editing


Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 2 of 7
* Yiping Qi, University of Maryland, USA
Expanding the Targeting Range of CRISPR in Plants


Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 3 of 7
Tobias Jores, University of Washington, USA
Learning Properties of Regulatory Elements for Future Crop Engineering


Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)
This session is from Plant Genome Engineering



Coffee Break


Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 5 of 7
William J. Gordon-Kamm, Corteva Agriscience, USA
The Status of Cereal Crop Transformation and Meeting Future Demands for Genome Modification


Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 6 of 7
Tom Adams, Pairwise, USA
How CRISPR Technology Can Help Us All Eat More Fruits and Vegetables


Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)
This session is from Plant Genome Engineering


Speaker 7 of 7
Rammyani Bagchi, University of North Carolina, Greensboro, USA
Short Talk: Use of Polyvalent Guide RNAs for CRISPR Antivirals


Tuesday, March 09 | 5:50PM - 6:00PM
Closing Remarks (5:50pm Denver/Mountain Time Start)
This session is from Plant Genome Engineering

Speaker 1 of 1
Caixia Gao, Chinese Academy of Sciences, China


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 1 of 9
* Daniel J. Siegwart, University of Texas Southwestern Medical Center, USA
Selective ORgan Targeting (SORT): A Synthetic Lipid Nanoparticle (LNP) Strategy for Effective Tissue-Specific Genome Engineering

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 2 of 9
* Charles Gersbach, Duke University, USA
In Vivo Editing Approaches for Gene Therapy and Functional Genomics

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 3 of 9
James Dahlman, Georgia Tech / Emory Medical School, USA
Delivering Gene Editing Components by Testing Thousands of Nanoparticles in vivo

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)


Coffee Break

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 5 of 9
Peter Deng, University of California, Davis, USA
Short Talk: Mesenchymal Stem Cell Delivery for Zinc Finger Proteins in Preclinical Animal Models of Angelman Syndrome

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 6 of 9
Sean Burns, Intellia Therapeutics, USA
Short Talk: In Vivo Genome Editing of Hematopoietic Stem and Progenitor Cells using Non-viral Delivery of CRISPR/Cas9

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 7 of 9
Aaron Lin, Metagenomi, USA
Short Talk: Broadening PAM Recognition of CRISPR-Associated Endonucleases by Protein Recombination

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 8 of 9
Kiara Berrios, University of Pennsylvania, USA
Short Talk: Controllable Genome Editing with Split-Engineered Base Editors

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)

Speaker 9 of 9
Kevin Hemphill, Horizon Discovery, USA
Short Talk: CRISPR-mediated Transcriptional Activation and Simultaneous Gene Knockout and Activation with Synthetic Guide RNAs

Wednesday, March 10 | 12:00PM - 1:30PM
Networking Lounge (Joint) (12pm Denver/Mountain Time Start)


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 1 of 9
* John G. Doench, Broad Institute of MIT and Harvard University, USA
Bridging the Variant-to-Function Chasm: Screens with Base Editor Technology

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 2 of 9
* Britt S. Adamson, Princeton University, USA
Single Cell Sequencing Technologies

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 3 of 9
Hyongbum Henry Kim, Yonsei University College of Medicine, South Korea
Predicting the Efficiencies and Outcomes of Genome Editing using Deep Learning

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)



Coffee Break

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 5 of 9
Jonathan S. Weissman, Whitehead Institute, HHMI, and MIT, USA
Genome-Wide Programmable Transcriptional Memory by CRISPR-Based Epigenome Editing

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 6 of 9
Alberto Ciccia, Columbia University, USA
Short Talk: Functional Interrogation of DNA Damage Response Variants with Base Editing Screens

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 7 of 9
Brian Cosgrove, Duke University, USA
Short Talk: Harnessing Epigenetic Editing Tools to Identify and Modulate Mechanically-Activated Genomic Enhancers

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 8 of 9
Pratiksha Thakore, Broad Institute, USA
[NOT AVAILABLE ON DEMAND] Short Talk: Toward Genome-Scale Single-Cell Screens with Multi-Modal Perturb-Seq

Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)


Speaker 9 of 9
Sarah Elisabeth Pierce, Stanford University, USA
Short Talk: Spear-ATAC: Pooled, Droplet-Based, Single-Cell Chromatin Accessibility CRISPR Screens

Wednesday, March 10 | 5:50PM - 6:00PM
Closing Remarks (5:50pm Denver/Mountain Time Start)


*Session Chair.