Web Desc
Plant Genome Engineering: From Lab to Field - VIRTUAL CONTENT AVAILABLE ON DEMAND
joint with Precision Engineering of the Genome, Epigenome and Transcriptome - VIRTUAL CONTENT AVAILABLE ON DEMAND
Organizer(s): Caixia Gao, Daniel F. Voytas and Holger Puchta
Date: March 08 - 10, 2021
Location: Virtual at your computer. Recorded presentation content is available On Demand.
Supported by the Directors' Fund
For important information on the coronavirus, please click here
Summary of Meeting:
Improvements in plant breeding are necessary to feed a growing population on a warming globe. CRISPR/Cas technology, which is revolutionizing molecular biology, has the potential to create a novel, sustainable agriculture. To date, thousands of plant biologists have started to use gene editing strategies; the time is ripe for a conference solely dedicated to CRISPR/Cas applications in plants. Topics covered will include the development of new tools for gene and base editing, gene targeting by homologous recombination, transcriptional regulation, epigenetic editing and induced chromosomal rearrangements. To apply these tools in plants, specific biological requirements have to be taken into account such as efficient transformation and regeneration procedures or organ specific expression. Trait improvements in crop plants - such as tomato, rice, wheat and corn - have been achieved and more are in the pipeline. The conference will be a platform for scientists from academia and industry to interact. It will also bring together scientists that work on tool development, plant transformation, plant breeding and crop trait development. Thus, not only will the newest applications of CRISPR/Cas in basic plant biology be offered, but the ground will be laid for a swift transfer of the benefits of the technology to that field.
Scholarship Deadline: November 1 2020
Abstract Deadline: February 16 2021
We gratefully acknowledge additional support for this conference from:
DCVC Bio
We gratefully acknowledge the generous grant for this conference provided by:

National Science Foundation (NSF)
Grant No. IOS_1942422
Funding for this conference was made possible (in part) by IOS_1942422 from the National Science Foundation. The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the National Science Foundation.
We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations
Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

Click here to view more of these organizations

Program

Monday, March 08 | 8:00AM - 8:40AM
Welcome and Keynote Address (8am Denver/Mountain Time Start)

Speaker 1 of 4
* Caixia Gao, Chinese Academy of Sciences, China

Monday, March 08 | 8:00AM - 8:40AM
Welcome and Keynote Address (8am Denver/Mountain Time Start)

Speaker 2 of 4
* Daniel F. Voytas, University of Minnesota, USA

Monday, March 08 | 8:00AM - 8:40AM
Welcome and Keynote Address (8am Denver/Mountain Time Start)

Speaker 3 of 4
* Holger Puchta, Karlsruhe Institute of Technology, Germany

Monday, March 08 | 8:00AM - 8:40AM
Welcome and Keynote Address (8am Denver/Mountain Time Start)

Speaker 4 of 4
Pamela C. Ronald, University of California, Davis, USA
Enhancing Food Security through Rice Genetic Improvement

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)


Speaker 1 of 7
* Daniel F. Voytas, University of Minnesota, USA
Overcoming Bottlenecks in Editing Plant Genomes

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)


Speaker 2 of 7
Feng Zhang, University of Minnesota, USA
Improving Scalable and Precision Genome Editing in Plants

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)



Coffee Break

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)


Speaker 4 of 7
* Holger Puchta, Karlsruhe Institute of Technology, Germany
CRISPR/Cas Mediated Plant Chromosome Engineering

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)


Speaker 5 of 7
Avraham A. Levy, Weizmann Institute of Science, Israel
From a DNA Break to Targeted Recombination between Homologous Chromosomes in Plants

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)


Speaker 6 of 7
Ayumu Takatsuka, Tohoku University, Japan
Short Talk: MitoTALEN-Mediated Mitochondrial Gene-Knockout Revealed a Cytoplasmic Male Sterility-Causative Gene in Oryza Sativa cv. Tadukan

Monday, March 08 | 8:40AM - 11:00AM
Plant Genome Editing Tools and Technology Development I (8:4
0am Denver/Mountain Time Start)


Speaker 7 of 7
Shin-ichi Arimura, University of Tokyo, Japan
Short Talk: Targeted Base Editing in the Plastid Genome of Arabidopsis thaliana

Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)
This session is from Precision Genome Engineering


Speaker 1 of 7
* Samuel H. Sternberg, Columbia University, USA
Targeted DNA Integration using CRISPR RNA-Guided Transposases


Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)
This session is from Precision Genome Engineering


Speaker 2 of 7
Cecilia Cotta-Ramusino, Tessera Therapeutics, USA
[NOT AVAILABLE ON DEMAND] New Editing Technologies


Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)
This session is from Precision Genome Engineering



Coffee Break


Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)
This session is from Precision Genome Engineering


Speaker 4 of 7
* Patrick D. Hsu, University of California, Berkeley, USA
CRISPR in the Time of COVID


Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)
This session is from Precision Genome Engineering


Speaker 5 of 7
Prashant Mali, University of California, San Diego, USA
[NOT AVAILABLE ON DEMAND] Endogenous RNA Editing Systems


Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)
This session is from Precision Genome Engineering


Speaker 6 of 7
Balint Csorgo, UCSF, USA
Short Talk: A Compact Cascade-Cas3 System for Targeted Genome Engineering and Gene Discovery


Monday, March 08 | 8:40AM - 11:00AM
Engineering of Editors and Epi-Editors (8:30am Denver/Mounta
in Time Start)
This session is from Precision Genome Engineering


Speaker 7 of 7
Yarui Diao, Duke University School of Medicine, USA
Short Talk: CRISPR-based Multimodal Functional Analysis Reveals the Key Role of Retroviral Sequence LTR7 in Governing Neural Fate Commitment of Human Embryo Development


Monday, March 08 | 12:30PM - 1:30PM
Career Roundtable (Joint) (12:30pm Denver/Mountain Time Star
t)

Speaker 1 of 3
Britt S. Adamson, Princeton University, USA

Monday, March 08 | 12:30PM - 1:30PM
Career Roundtable (Joint) (12:30pm Denver/Mountain Time Star
t)

Speaker 2 of 3
Rachel E. Haurwitz, Caribou Biosciences, Inc., USA

Monday, March 08 | 12:30PM - 1:30PM
Career Roundtable (Joint) (12:30pm Denver/Mountain Time Star
t)

Speaker 3 of 3
Steven E. Jacobsen, University of California, Los Angeles, USA

Monday, March 08 | 1:30PM - 3:00PM
Poster Session 1 (Joint) (1:30pm Denver/Mountain Time Start)


Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)


Speaker 1 of 8
* Steven E. Jacobsen, University of California, Los Angeles, USA
Epigenetic Editing in Plants

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)


Speaker 2 of 8
Seiichi Toki, National Agriculture and Food Research Organization, Japan
Plant DNA Repair and Genome Engineering

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)


Speaker 3 of 8
Erika Toda, Tokyo Metropolitan University, Japan
An Efficient DNA- and Selectable-Marker-Free Genome-Editing System Using Zygotes in Rice and Its Possible Application to Other Crop Species

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)



Coffee Break

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)


Speaker 5 of 8
* Caixia Gao, Chinese Academy of Sciences, China
Precise Prime Editing in Plants

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)


Speaker 6 of 8
Lanqin Xia, Chinese Academy of Agricultural Sciences, China
CRISPR/Cas-Mediated Precision Genome Editing for Crop Improvement

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)


Speaker 7 of 8
Jian-Kang Zhu, Shanghai Center for Plant Stress Biology, CAS, China
Strategies for Efficient Gene Targeting in Plants

Monday, March 08 | 3:00PM - 6:00PM
Plant Genome Editing Tools and Technology Development II (3p
m Denver/Mountain Time Start)


Speaker 8 of 8
Keishi Osakabe, Tokushima University, Japan
Short Talk: Genome Editing Using the CRISPR Type I-D Nuclease in Plants

Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 1 of 10
* Charles Gersbach, Duke University, USA


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 2 of 10
Jacob E. Corn, ETH Zürich, Switzerland
Visible and Invisible HDR in Human Cells


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 3 of 10
Bas van Steensel, Netherlands Cancer Institute, Netherlands
Multiplexed Probing of the Impact of Chromatin on Cas9-Induced DSB Repair Pathways


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 4 of 10
Ahmad S. Khalil, Boston University, USA
Design of Eukaryotic Gene Regulatory Circuits


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering


Coffee Break


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 6 of 10
Jennifer E. Phillips-Cremins, University of Pennsylvania, USA
Engineering the Repetitive 3D Genome in Human Disease


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 7 of 10
Alyna Katti, Weill Cornell Medicine, USA
Short Talk: Strategies to Improve Engineering Cancer Associated SNVs with Base Editing


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 8 of 10
Daniel M. Sapozhnikov, McGill University, Canada
Short Talk: Unraveling the Transcriptional Impact of DNA Demethylation at Specific Promoters by Targeted Steric Blockage of DNA Methyltransferase with CRISPR/dCas9


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 9 of 10
* Henriette O'Geen, University of California, Davis, USA
Short Talk: Determinants of Persistence in Epigenetic Editing


Monday, March 08 | 3:00PM - 6:00PM
Controlling CRISPR (3pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 10 of 10
Isaac B. Hilton, Rice University, USA
Short Talk: Programmable Human Histone Phosphorylation and Gene Activation using a CRISPR/Cas9-Based Chromatin Kinase


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 1 of 9
* Zachary Lippman, Howard Hughes Medical Institute/Cold Spring Harbor Laboratory, USA
Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 2 of 9
* Jens Boch, Leibniz Universität Hannover, Germany
Expanding the TALEN Toolbox for Genome Editing

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 3 of 9
Joyce Van Eck, Boyce Thompson Institute, USA
Exploring Insect Interactions in Physalis Using CRISPR Generated Mutant Lines

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)



Coffee Break

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 5 of 9
Magdy Mahfouz, King Abdullah University of Science and Technology, Saudi Arabia
RNA-Virus Interference via CRISPR-Cas13 Systems

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 6 of 9
Mariette Andersson, Swedish University of Agricultural Sciences, Sweden
Genome Editing for Improved Potato Traits


Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 7 of 9
Eyal Fridman, Volcani Center ARO, Israel
Short Talk: RECAS9-Mediated QTL Mapping by Mitotic Genome Editing in Cereals

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 8 of 9
Sergei Svitashev, Corteva Agriscience, USA
Short Talk: CRISPR-Cas9-mediated 75.5 Mb Inversion in Maize

Tuesday, March 09 | 8:00AM - 11:00AM
Application of Genome Editing in Agriculture I (8am Denver/M
ountain Time Start)


Speaker 9 of 9
Trevor Weiss, University of Minnesota, USA
Short Talk: Differential CRISPR/Cas9 Genome Editing Influenced by Epigenetic Factors

Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 1 of 9
* Rachel E. Haurwitz, Caribou Biosciences, Inc., USA
The Future is Allogeneic - Translating a CRISPR Platform into Gene-Edited Cell Therapies


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 2 of 9
Matthew Porteus, Stanford University School of Medicine, USA
Genome Editing of Blood Cells to Cure Disease


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 3 of 9
Chiara Bonini, Vita-Salute San Raffaele University, Italy
T-Cell Gene Editing for Cancer Immunotherapy


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering



Coffee Break


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 5 of 9
* Nicole Gaudelli, Beam Therapeutics, USA
An Adenine Base Editing Strategy for the Treatment of Sickle Cell Disease by Elimination of the Pathogenic Globin Protein


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 6 of 9
Delphine Guipouy, CHU Sainte Justine Research Center, Canada
[NOT AVAILABLE ON DEMAND] Short Talk: Knockout of NKG2A by CRISPR in Human NK Cells Enhance their Cytotoxic Activity Against Solid Tumors


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 7 of 9
Dimitrios Laurin Wagner, Charité - Universitätsmedizin Berlin, Germany
Short Talk: Fast and Efficient Generation of TRAC-integrated CAR T Cells without Viruses


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 8 of 9
Adrian Veres, Harvard University, USA
Short Talk: Optimizing Stem Cell Lines for in vitro-derived Beta Cell Therapeutics via Genome-wide CRISPR Knock-out Screening


Tuesday, March 09 | 8:00AM - 11:00AM
Therapeutics I: Ex Vivo Editing (8am Denver/Mountain Time St
art)
This session is from Precision Genome Engineering


Speaker 9 of 9
Sébastien Levesque, CHU de Québec - Université Laval, Canada
Short Talk: Expanding the Scope of Marker-Free Selection for CRISPR-Driven Genome Editing in Human Cells


Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 1 of 6
Roland George Roberts, Public Library of Science, UK

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 2 of 6
Holger Matthias Breithaupt, EMBO, Germany

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 3 of 6
Kevin Davies, CRISPR Journal, USA

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 4 of 6
Bing Yang, Frontiers in Genome Editing, USA

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 5 of 6
Ruth Zearfoss, Cell Press, USA

Tuesday, March 09 | 12:30PM - 1:30PM
Meet the Editors (Joint) (12:30pm Denver/Mountain Time Start
)

Speaker 6 of 6
Caitlin Karniski, Nature Portfolio, USA

Tuesday, March 09 | 1:30PM - 3:00PM
Poster Session 2 (Joint) (1:30pm Denver/Mountain Time Start)


Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)


Speaker 1 of 7
* Kan Wang, Iowa State University, USA
Breeding of a Transformable Model Maize Line for Genome Editing

Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)


Speaker 2 of 7
* Yiping Qi, University of Maryland, USA
Expanding the Targeting Range of CRISPR in Plants

Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)


Speaker 3 of 7
Tobias Jores, University of Washington, USA
Learning Properties of Regulatory Elements for Future Crop Engineering

Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)



Coffee Break

Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)


Speaker 5 of 7
William J. Gordon-Kamm, Corteva Agriscience, USA
The Status of Cereal Crop Transformation and Meeting Future Demands for Genome Modification

Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)


Speaker 6 of 7
Tom Adams, Pairwise, USA
How CRISPR Technology Can Help Us All Eat More Fruits and Vegetables

Tuesday, March 09 | 3:00PM - 5:50PM
Application of Genome Editing in Agriculture II (3pm Denver/
Mountain Time Start)


Speaker 7 of 7
Rammyani Bagchi, University of North Carolina, Greensboro, USA
Short Talk: Use of Polyvalent Guide RNAs for CRISPR Antivirals

Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering


Speaker 1 of 8
David G. Ousterout, Locus Biosciences, Inc., USA
Development of crPhage to Treat Infections


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering


Speaker 2 of 8
* Fyodor D. Urnov, University of California, Berkeley, USA
Towards a CRISPRi/a Therapeutic for Radiation Injury


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering


Speaker 3 of 8
Aravind Asokan, Duke University, USA
Engineering AAV for Targeted In Vivo Editing


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering



Coffee Break


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering


Speaker 5 of 8
* Ana Moreno Collado, Navega Therapeutics, USA
Epigenome Regulation for Treatment of Chronic Pain


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering


Speaker 6 of 8
Chikdu Shivalila, Wave Life Sciences, USA
Short Talk: A Versatile Platform for ADAR-Mediated RNA Editing in vivo in Preclinical Models


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering


Speaker 7 of 8
Jia Qi Cheng-Zhang, University of Pittsburgh, USA
Short Talk: Upregulation of Disease Compensatory Gene via CRISPR Activation in Muscular Dystrophy Mice


Tuesday, March 09 | 3:00PM - 6:00PM
Therapeutics II: In Vivo Editing (3pm Denver/Mountain Time S
tart)
This session is from Precision Genome Engineering


Speaker 8 of 8
Serena Tamura, University of California San Francisco, USA
Short Talk: CRISPR Activation to Rescue SCN2A Haploinsufficiency in Autism Spectrum Disorder


Tuesday, March 09 | 5:50PM - 6:00PM
Closing Remarks (5:50pm Denver/Mountain Time Start)

Speaker 1 of 1
Caixia Gao, Chinese Academy of Sciences, China

Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 1 of 9
* Daniel J. Siegwart, University of Texas Southwestern Medical Center, USA
Selective ORgan Targeting (SORT): A Synthetic Lipid Nanoparticle (LNP) Strategy for Effective Tissue-Specific Genome Engineering


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 2 of 9
* Charles Gersbach, Duke University, USA
In Vivo Editing Approaches for Gene Therapy and Functional Genomics


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 3 of 9
James Dahlman, Georgia Tech / Emory Medical School, USA
Delivering Gene Editing Components by Testing Thousands of Nanoparticles in vivo


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering


Coffee Break


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 5 of 9
Peter Deng, University of California, Davis, USA
Short Talk: Mesenchymal Stem Cell Delivery for Zinc Finger Proteins in Preclinical Animal Models of Angelman Syndrome


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 6 of 9
Sean Burns, Intellia Therapeutics, USA
Short Talk: In Vivo Genome Editing of Hematopoietic Stem and Progenitor Cells using Non-viral Delivery of CRISPR/Cas9


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 7 of 9
Aaron Lin, Metagenomi, USA
Short Talk: Broadening PAM Recognition of CRISPR-Associated Endonucleases by Protein Recombination


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 8 of 9
Kiara Berrios, University of Pennsylvania, USA
Short Talk: Controllable Genome Editing with Split-Engineered Base Editors


Wednesday, March 10 | 8:00AM - 11:00AM
Delivery (8am Denver/Mountain Time Start)
This session is from Precision Genome Engineering

Speaker 9 of 9
Kevin Hemphill, Horizon Discovery, USA
Short Talk: CRISPR-mediated Transcriptional Activation and Simultaneous Gene Knockout and Activation with Synthetic Guide RNAs


Wednesday, March 10 | 12:00PM - 1:30PM
Networking Lounge (Joint) (12pm Denver/Mountain Time Start)


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 1 of 9
* John G. Doench, Broad Institute of MIT and Harvard University, USA
Bridging the Variant-to-Function Chasm: Screens with Base Editor Technology


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 2 of 9
* Britt S. Adamson, Princeton University, USA
Single Cell Sequencing Technologies


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 3 of 9
Hyongbum Henry Kim, Yonsei University College of Medicine, South Korea
Predicting the Efficiencies and Outcomes of Genome Editing using Deep Learning


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering



Coffee Break


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 5 of 9
Jonathan S. Weissman, Whitehead Institute, HHMI, and MIT, USA
Genome-Wide Programmable Transcriptional Memory by CRISPR-Based Epigenome Editing


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 6 of 9
Alberto Ciccia, Columbia University, USA
Short Talk: Functional Interrogation of DNA Damage Response Variants with Base Editing Screens


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 7 of 9
Brian Cosgrove, Duke University, USA
Short Talk: Harnessing Epigenetic Editing Tools to Identify and Modulate Mechanically-Activated Genomic Enhancers


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 8 of 9
Pratiksha Thakore, Broad Institute, USA
[NOT AVAILABLE ON DEMAND] Short Talk: Toward Genome-Scale Single-Cell Screens with Multi-Modal Perturb-Seq


Wednesday, March 10 | 3:00PM - 5:50PM
Screening & Single Cell Technologies (3pm Denver/Mountain Ti
me Start)
This session is from Precision Genome Engineering


Speaker 9 of 9
Sarah Elisabeth Pierce, Stanford University, USA
Short Talk: Spear-ATAC: Pooled, Droplet-Based, Single-Cell Chromatin Accessibility CRISPR Screens


Wednesday, March 10 | 5:50PM - 6:00PM
Closing Remarks (5:50pm Denver/Mountain Time Start)
This session is from Precision Genome Engineering


*Session Chair.