Registration (4:00–8:00 PM)
Welcome Mixer (6:00–8:00 PM)
Poster Setup (8:00–8:30 AM)
Poster Viewing (8:00–5:00 PM)
Welcome and Keynote Address (8:30–9:30 AM)
* Sharon B. Cantor, University of Massachusetts Chan Medical School
Session Chair
Helle D. Ulrich, Institute of Molecular Biology
Mind the Gap: How Ubiquitylation Controls Postreplicative Gap Formation and Repair in Response to DNA Replication Stress
DNA Replication Gaps: Causes and Consequences (9:30–12:00 PM)
* Vanesa M. Gottifredi, Fundación Instituto Leloir
Session Chair
* Annabel Quinet, Francois Jacob Institute of Biology, CEA/INSERM
Session Chair
Vincenzo Costanzo, IFOM
Causes and Consequences of DNA Replication Gaps
Alessandro Vindigni, Washington University
Factors Modulating Gap Formation and Repair
Aura Carreira, Centro de Biologia Molecular Severo Ochoa (CBM)
Mediating Replication ssDNA Gap Prevention and Repair Through the Lenses of BRCA2
Catherine H Freudenreich, Tufts University
Gap-Induced Fragility at DNA Structures Revealed in a Yeast Model
Coffee Break (10:00–10:20 AM)
Poster Session 1 (12:00–2:00 PM)
Symposia Spotlight 1: Short Talks Chosen from Abstracts (2:30–4:30 PM)
* Catherine H Freudenreich, Tufts University
Session Chair
* Aura Carreira, Centro de Biologia Molecular Severo Ochoa (CBM)
Session Chair
Gary Ying Wai Chan, The University of Hong Kong
FANCM Minimizes Single-Strand Gap Formation and Promotes PARP Inhibitor Resistance by Counteracting 53BP1
So Young Joo, Seoul National University
BRCA2 at the Helm: Orchestrating Telomere G4 Dynamicity and its Impact in Ovarian Cancer
Ja Yil Lee, Ulsan National Institute of Science and Technology
Alteration of RPA Binding Modes on Single-Stranded DNA by NSMF Potentiates RPA Phosphorylation by ATR Kinase
Junko Murai, Ehime University
SLFN11 Enhances Chemotherapeutic Sensitivity by Binding to Multiple Types of Single-Stranded DNA Gaps
Yonghwan Kim, Sookmyung Women's University
RNF4 and USP7 Coordinate Spatial Regulation of SLX4 Stability within the PML Nuclear Bodies
Isabelle Seppa, Washington University in Saint Louis
Mechanisms of ssDNA Gap Resection in BRCA1-Proficient and -Deficient Cells
Helen E Grimsley, Memorial Sloan Kettering
The 9-1-1 Complex Protects ssDNA gaps in BRCA2-Deficient Cancer Cells To Preserve Cell Viability
Yohan An, KAIST
APOBEC3A, not APOBEC3B, Drives Deaminase-Associated Mutagenesis In Single-Stranded DNA Gaps in Human Gastric Epithelium
Coffee Available (4:30–5:00 PM)
Gaps, Cell Death and Immune Responses (5:00–7:00 PM)
* Alessandro Vindigni, Washington University
Session Chair
* Katharina Schlacher, MD Anderson Cancer Center
Session Chair
Sharon B. Cantor, University of Massachusetts Chan Medical School
How the Gap Model Redefines Chemotherapy Response
Roger Greenberg, University of Pennsylvania
Tolerance to Stress vs. Immune Induction
Vanesa M. Gottifredi, Fundación Instituto Leloir
Causes and Consequences of the Choice between Template Switching, Traslesion DNA Synthesis and Repriming
Hervé Técher, Université Côte d'Azur
Short Talk: MRE11 Nuclease Activity Controls Senescence by Coordinating Replication Stress And Interferon Signaling
Dmitry Ivanov, Institute for Basic Science
Short Talk: The Role of Translesion Synthesis (TLS) Polymerases in Temozolomide Resistance
On Own for Dinner (7:00–8:00 PM)
Poster Setup (8:00–8:30 AM)
Poster Viewing (8:00–5:00 PM)
Visualizing and Defining the Gap Architecture (8:30–11:30 AM)
* Dale A. Ramsden, University of North Carolina
Session Chair
Maria Spies, University of Iowa
Structure of the RAD52 double-ring at stalled replication forks, restriction of fork reversal and prevention of replication gaps
Katharina Schlacher, MD Anderson Cancer Center
A BRCA Replication Fork Protecteome in Inflammation and Disease
Nitika Taneja, Erasmus Medical Center
Local & Spatial Chromatin Reorganization under Replication Stress
Annabel Quinet, Francois Jacob Institute of Biology, CEA/INSERM
Central Role of Repriming by PRIMPOL in the Response To Replication Stress Induced By Low Doses of Genotoxic Agents
Maximilian Donsbach, Ludwig Maximilians University Munich
Short Talk: A Non-Proteolytic Release Mechanism for HMCES-DNA-Protein Crosslinks
Jinho Park, Stanford University
Short Talk: Dissecting the Mechanism of Repriming during DNA Damage Response Using Single-Molecule Imaging
Coffee Break (9:30–9:50 AM)
Award Recipient Acknowledgement (9:50–9:55 AM)
Poster Session 2 (12:00–2:00 PM)
Panel Discussion: Brainstorming Gap Detection Tools (2:15–3:15 PM)
Vincenzo Costanzo, IFOM
Alessandro Vindigni, Washington University
Nitika Taneja, Erasmus Medical Center
David Szuts, HUN-REN Research Centre for Natural Sciences
Joanna Loizou, The Institute of Cancer Research
Career Roundtable (3:30–4:30 PM)
Karlene A Cimprich, Stanford University
Professor
Kei-ichi Takata, Institute for Basic Science
Section Head
Nele Hug, Nature Communications
Senior Editor
Coffee Available (4:30–5:00 PM)
Gap Suppression and Avoidance Pathways in Cancer (5:00–7:00 PM)
* Karlene A Cimprich, Stanford University
Session Chair
* Fumiko Esashi, University of Oxford
Session Chair
Alberto Ciccia, Columbia University
Dependency of ALT-Positive Cancer Cells on the SMARCAL1 DNA Translocase
Dale A. Ramsden, University of North Carolina
Pol Theta and the Response to Replication Stress
David Szuts, HUN-REN Research Centre for Natural Sciences
A Genetic Delineation of Template Switching Bypass and its Role in Spontaneous Mutagenesis
Diego Dibitetto, Istituto di Ricerche Farmacologiche Mario Negri
Short Talk: DNA-PK Counteracts Toxic Single-Strand Breaks Accumulation and Promotes PARP Inhibitor Resistance
Kei-ichi Takata, Institute for Basic Science
Short Talk: DNA Helicase HELQ Facilitates Fork Reversal in Response to Leading Strand Gaps
On Own for Dinner (7:00–8:00 PM)
Wednesday, April 30, 2025
Gaps, R-Loops and Replication Stress (8:30–11:30 AM)
* Maria Spies, University of Iowa
Session Chair
* Roger Greenberg, University of Pennsylvania
Session Chair
Kristijan Ramadan, Nanyang Technical University
The Role of Nucleophagy in DNA Repair and Genome Stability
Fumiko Esashi, University of Oxford
Untangling DNA Breakage at Centromeres
Joanna Loizou, The Institute of Cancer Research
POLtheta Processes ssDNA Gaps and Promotes Replication Fork Progression in BRCA1-Deficient Cells
Karlene A Cimprich, Stanford University
Mechanisms for Rna-Mediated Genome Instability
Marie Sebald, The Francis Crick Insitute
Short Talk: SLX complex Promotes Okazaki Fragment Maturation in FEN1 Deficient Cells
Larissa Milano, University of Sussex
Short Talk: BRCA1 and BRCA2-Mediated Maturation of Nascent DNA Strands During DNA Replication
Coffee Break (9:30–9:50 AM)
On Own for Lunch (11:30–2:30 PM)
Symposia Spotlight 2: Short Talks Chosen from Abstracts (2:30–4:30 PM)
* David Szuts, HUN-REN Research Centre for Natural Sciences
Session Chair
* Orlando D Schärer, University of Pittsbrugh
Session Chair
Kyunghwan Kim, IBS
Enhanced Precision Targeting of Cancer Cells Using the CRISPR/Cas9 System and Lipid Nanoparticles
Seung Woo Cho, UNIST
Efficient and precise targeting of cancer cells using multiplexed CRISPR/Cas9-nickase
Jieya Shao, Washington University in St. Louis
Moonlighting Nuclear Function of The Actin-Binding Factor Profilin-1 In ssDNA Gap Formation
Hyunje Kang, Stanford university
Fork Reversal Prevents TRAIP-Mediated POLE1 Degradation Under Conditions of ROS
Néstor García-Rodríguez, CABIMER/University of Sevilla
The Role of ssDNA Gap Expansion in ATR Checkpoint Activation and Synthetic Lethality in BRCA1-defcient Cells
John J Turchi, Indiana University School of Medicine
Chemical Inhibition of RPA Gap Protection Sensitizes BRCA1-deficient Cancers to PARP Inhibition
Pauline Luise Pfuderer, University of Cambridge
Artificial Intelligence-based High-resolution Analysis of DNA Replication Dynamics on Extrachromosomal DNA
Ronnie Low, The Francis Crick Institute
MEN1 Loss Confers Resistance to Replication Stress-Inducing Drugs
Coffee Available (4:30–5:00 PM)
Drugs Modulating and Capitalizing on a Cancer Gap Vulnerability (5:00–6:30 PM)
* Joanna Loizou, The Institute of Cancer Research
Session Chair
* Isabelle Seppa, Washington University in Saint Louis
Session Chair
Orlando D Schärer, University of Pittsbrugh
Trabectedin - Targeting Tumors through Transcription-Coupled Nucleotide Excision Repair Induced DNA Gaps
Jill Bargonetti, Hunter College, City University of New York
p53 Gain of Function working together with PARP1 for Cancer Persistent Repair at Gaps
Kyungjae Myung, Institute for Basic Science and UNIST
Translation of DNA Damage Including Gap for Cancer Therapy
Meeting Wrap-Up: Outcomes and Future Directions (Organizers) (6:30–6:45 PM)
Social Hour with Lite Bites (6:45–7:45 PM)
Departure (12:00–11:59 PM)
