Granlibakken Tahoe Floorplan

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This meeting took place in 2018



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Cryo-EM from Cells to Molecules: Multi-Scale Visualization of Biological Systems (F1)


Organizer(s) Georgios Skiniotis, Elizabeth Villa and Andrew B. Ward
February 4—8, 2018
Granlibakken Tahoe • Tahoe City, California USA
Discounted Abstract Deadline: Oct 5, 2017
Abstract Deadline: Nov 7, 2017
Scholarship Deadline: Oct 5, 2017
Discounted Registration Deadline: Dec 7, 2017

Sponsored by Pfizer Inc.

Summary of Meeting:
Fast-evolving cryo-EM visualization techniques have recently started to illuminate the inner workings of complicated and dynamic biological assemblies with a level of detail we have never seen before. Cryo-EM is now the state-of-the-art methodology for taking snapshots of biological systems from low to high resolution and with various levels of complexities, from small macromolecular complexes all the way to subcellular organizations. This symposium will showcase cryo-EM breakthroughs in addressing crucial problems in cell biology and will further highlight cryo-EM imaging as a platform for the integration of data from orthogonal approaches. With talks on a variety of biological specimens, procedures and types of biological questions, the meeting aims to attract a very diverse audience from both academia and industry with interest in the method, and beyond the traditional cryo-EM specialist. The symposium will focus on four themes: 1) Single-particle cryo-EM with attention to the characterization of the conformational ensemble adopted by macromolecular complexes and aiming to delineate their mechanism of action; 2) Cryo-electron tomography (cryo-ET) and correlative light electron microscopy (CLEM) to visualize cellular organizations and their dynamics; 3) The emergence of cryo-EM as tool for drug discovery and translational research through both high-resolution structure determination and characterization of cellular phenotypes; and 4) Recent developments in sample preparation and image acquisition. Furthermore, the symposium will include workshops in specimen preparation and evaluation of suitability for cryo-EM, modeling of structures using cryo-EM data, and how to set up a cryo-EM lab.

View Scholarships/Awards
No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 12 hr (am/pm) time


The meeting will begin on Sunday, February 4 with registration from 16:00 to 20:00 and a welcome mixer and light meal from 18:00 to 20:00. Conference events conclude on Thursday, February 8 with a closing plenary session and keynote address from 17:00 to 19:00, followed by dinner and entertainment. We recommend return travel on Friday, February 9 in order to fully experience the meeting.

SUNDAY, FEBRUARY 4

16:00—20:00
Arrival and Registration

Pre Function
18:00—20:00
Welcome Mixer
No registration fees are used to fund alcohol served at this function.

Granhall
18:00—20:00
Light Meal - Provided by Granlibakken

Granhall

MONDAY, FEBRUARY 5

07:00—08:00
Breakfast

Granhall
08:00—09:00
Welcome and Keynote Address
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Georgios Skiniotis, Stanford University, USA

* Andrew B. Ward, The Scripps Research Institute, USA

* Elizabeth Villa, University of California, San Diego, USA

Patrick Cramer, Max Planck Institute for Biophysical Chemistry, Germany
Integrated Structural Biology of Gene Transcription

09:00—11:30
Visualizing Cells
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Elizabeth Villa, University of California, San Diego, USA
Opening Windows into the Cell: Bringing Structure to Cell Biology using Cryo-Electron Tomography

Coffee Break

Grant J. Jensen, California Institute of Technology, USA
Structure and Function of the Bacterial Type IV Secretion System through Electron Cryotomography

Mark H. Ellisman, University of California, San Diego, USA
Revealing Secrets Hiding in Plain Sight

Christina Heroven, MRC Laboratory of Molecular Biology, UK
Short Talk: In-situ Structural Studies of RPTPmu by Cryo-Electron Tomography of FIB Milled Cells

Kanika Khanna, University of California, San Diego, USA
Short Talk: Understanding Morphological Changes Accompanying Sporulation in B. subtilis using in situ Cryo-Electron Tomography

11:30—17:00
On Own for Lunch

11:30—13:00
Poster Setup

Bay
13:00—22:00
Poster Viewing

Bay
16:30—17:00
Coffee Available

Pre Function
17:00—19:00
Visualizing Subcellular Compartments
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
Daniela Nicastro, University of Texas Southwestern Medical Center, USA
Probing the Molecular Organization of Cells and Organelles using Cryo-Electron Microscopy

Jun Liu, Yale School of Medicine, USA
High-Throughput Cryo-Electron Tomography: Visualizing Host-Pathogen Interactions at High Resolution

* Kay Grünewald, University of Oxford, UK
Trafficking Vesicles and Enveloped Viruses

Joshua Hutchings, Birkbeck College, UK
Short Talk: Cryo-EM Tomography and Subtomogram Averaging of COPII Assembled on Membranes

19:00—20:00
Dinner

Granhall
19:30—22:00
Poster Session 1

Bay
20:00—21:00
Social Hour
No registration fees are used to fund alcohol served at this function.

Bay

TUESDAY, FEBRUARY 6

07:00—08:00
Breakfast

Granhall
08:00—11:15
Translational Cryo-EM
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Andrew B. Ward, The Scripps Research Institute, USA
Structure-Based Vaccine Design

Tom Ceska, UCB Celltech, UK
Cryo-EM as a Tool for Structure Determination in Industry

Coffee Break

Seungil Han, Pfizer Inc., USA
Applications of Cryo-EM in Small Molecule and Biologics Drug Design

Christian Wiesmann, Novartis, Switzerland
Cryo-Em: A New Tool for Drug Discovery at Novartis

Gregory Martin, Oregon Health & Science University, USA
Short Talk: Anti-Diabetic Drug Binding Site in KATP Channels Revealed by Cryo-EM

Chitra Shintre, University of Oxford, UK
Short Talk: Structure of the Polycistic Kidney Disease TRP Channel PC2

11:15—17:00
On Own for Lunch

11:15—13:00
Poster Setup

Bay
13:00—22:00
Poster Viewing

Bay
14:30—16:30
Workshop 1: Specimen Preparation and Troubleshooting for Single-Particle Work

Mountain/Lake
* Iban Ubarretxena, Icahn School of Medicine at Mount Sinai, USA
Structures of Key Intermediates in the Human Mitochondrial Chaperonin Reaction Cycle

Anika L. Burrell, University of Washington, USA
Human IMPDH1 Retinal Degeneration Mutations Promote Polymer Assembly

Joel Butterwick, Rockefeller University, USA
Cryo-EM Structure of Orco: The Molecular Basis for Insect Olfaction

Ziao Fu, Columbia University, USA
Lipid Bilayer Structure in Native Cell Membrane Nanoparticles of Multidrug Exporter AcrB

Jonas Barandun, Rockefeller University, USA
Structure of the Earliest Stable Precursor of the Ribosome - the Small Subunit Processome

Vignesh Kasinath, University of California, Berkeley, USA
Cryo-EM Structure of the Full Human PRC2 and Regulation by AEBP2 and JARID2

Qianhui Qu, Stanford University, USA
Structure of a Histone H3K4 Methyltransferase COMPASS

Julia Brasch, Columbia University, USA
Assembly of Clustered Protocadherin Neuronal Recognition Complexes Studied by X-ray Crystallography and Cryo-electron Tomography

Cristina Puchades, The Scripps Research Institute, USA
Hand-Over-Hand: How ATP Drives Protein Translocation

Harry Low, Imperial College, UK
Architecture of a Bacterial Type II Secretion System

16:30—17:00
Coffee Available

Pre Function
17:00—19:00
Frontiers in Cryo-EM
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
Andrej Sali, University of California, San Francisco, USA
Integrative Modeling of Biomolecular Assembly Structures and Pathways

* Sharon Grayer Wolf, Weizmann Institute of Science, Israel
Deep Views into Vitrified Cells by Cryo-Scanning Transmission Electron Tomography

David Baker, University of Washington, USA
De novo Design of Self-Assembling Protein Nano Materials

Yuxi Liu, University of California, Los Angeles, USA
Short Talk: Near-Atomic Resolution Cryo-EM for a Small Protein on a Symmetric Imaging Scaffold

19:00—20:00
Dinner
No registration fees are used to fund alcohol served at this function.

Granhall
19:30—22:00
Poster Session 2

Bay
20:00—21:00
Social Hour

Bay

WEDNESDAY, FEBRUARY 7

07:00—08:00
Breakfast

Granhall
08:00—11:00
Cryo-EM of Small Single Particles
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
Dmitry Lyumkis, The Salk Institute for Biological Studies, USA
High-Resolution Cryo-EM Studies of Retroviral Intasomes

Maofu Liao, Harvard Medical School, USA
Single Particle Cryo-EM Studies of Lipid-Protein Interactions

Coffee Break

* Gabriel C. Lander, The Scripps Research Institute, USA
How Low Can You Go? Size and Resolution Limits using Conventional Cryo-EM

Moran Shalev-Benami, Stanford University, USA
Short Talk: Cryo-EM Structure of the Synaptic Wiring Protein Teneurin

Christopher P. Hill, University of Utah, USA
Short Talk: Structural Basis for Protein Translocation by the Vps4 AAA ATPase

Daniel Southworth, University of California, San Francisco, USA
Short Talk: Visualizing the Substrate Translocation Mechanism of the Hsp104 AAA+ Disaggregase

11:00—17:00
On Own for Lunch

11:00—13:00
Poster Setup

Bay
13:00—22:00
Poster Viewing

Bay
14:30—16:30
Workshop 2: Modeling and Refining Structures of Macromolecules in Cryo-EM Maps

Mountain/Lake
* Gunnar Schroeder, Forschungszentrum Julich, Germany
Fibril Structure of Amyloid-ß(1-42) by Cryo-Electron Microscopy

Jane S. Richardson, Duke University, USA
CaBLAM - A New Tool Designed to Validate 2.5-4Å Models from CryoEM

Doo Nam Kim, Los Alamos National Laboratory, USA
CryoFIT: Flexible Fitting of Biomolecule Structures from Cryo-EM Maps with User-Friendly Molecular Dynamics Simulation

Jianpeng Ma, Baylor College of Medicine, USA
Application of Parallel Continuous Simulated Tempering to Cryo-EM Structural Refinement at Near-Atomic Resolution

Abhishek Singharoy, Arizona State University, USA
Molecular Dynamics Flexible Fitting at sub-5 Å Resolution

Till Rudack, Ruhr University Bochum, Germany
ModelMaker: An Interactive Integrative Modeling Tool to Build Complete Macromolecular Machines

Kelly Brock, Harvard Medical School, USA
Using Evolutionary Couplings to Help Build and Refine Cryo-EM Models

16:30—17:00
Coffee Available

Pre Function
17:00—19:15
Cryo-EM of Large Macromolecular Assemblies
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
Kiyoshi Nagai, Medical Research Council, UK
Cryo-EM Snapshots of the Spliceosome Provide Insights into the Mechanism of pre-mRNA Splicing

Gira Bhabha, New York University School of Medicine, USA
Architectures of Lipid Transport Systems in Bacteria

* Adam Frost, University of California, San Francisco, USA
Function Follows Form: Cryo-EM Insights into Cell Biology

Eva Nogales, University of California, Berkeley, USA
Challenges and Progress in the Study of Large Human Complexes in Gene Expression Regulation

19:15—20:15
Dinner
No registration fees are used to fund alcohol served at this function.

Granhall
19:30—22:00
Poster Session 3

Bay
20:00—21:00
Social Hour

Bay

THURSDAY, FEBRUARY 8

07:00—08:00
Breakfast

Granhall
08:00—11:15
Cryo-EM of Membrane Proteins
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Georgios Skiniotis, Stanford University, USA
Cryo-EM Visualization of G Protein-Coupled Receptors

Judy Hirst, University of Cambridge, UK
CryoEM Structure of Mammalian Respiratory Complex I, an Asymmetric 1 MDa Energy-Converting Enzyme

Coffee Break

Doreen Matthies, National Institutes of Health, USA
Single Particle Cryo-EM of a 200 kDa Magnesium Ion Channel

Eric Gouaux, Oregon Health & Science University, USA
Structure and Mechanism of AMPA Receptor - Auxiliary Protein Complexes

Kei Saotome, The Scripps Research Institute, USA
Short Talk: Structure of the Mechanically Activated Ion Channel Piezo1

Luke H. Chao, Massachusetts General Hospital, USA
Short Talk: Single Particle Cryo-EM Studies of Mitochondrial Inner-Membrane Fusion

11:15—17:00
On Own for Lunch

16:30—17:00
Coffee Available

Pre Function
17:00—18:00
Recent Technological Advances in Cryo EM
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
Radostin Danev, Max Planck Institute of Biochemistry, Germany
Single Particle Imaging with the Volta Phase Plate

* Bridget Carragher, New York Structural Biology Center, USA
Sample Vitrification Issues and Some Ideas about How to Overcome Them

18:00—18:45
Closing Keynote Address
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
* Georgios Skiniotis, Stanford University, USA

Joachim Frank, Columbia University, USA
Story in a Sample: Multiple Ribosome Structures in a Single Cryo-EM Experiment

18:45—19:00
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)
Meeting has ended...abstracts no longer viewable online.

Mountain/Lake
19:00—20:00
Dinner

Granhall
19:00—20:00
Social Hour

Granhall
20:00—23:00
Entertainment
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.

Granhall

FRIDAY, FEBRUARY 9

 
Departure


*Session Chair †Invited, not yet responded.



We gratefully acknowledge support for this conference from:


Directors' Fund


These generous unrestricted gifts allow our Directors to schedule meetings in a wide variety of important areas, many of which are in the early stages of research.

Click here to view all of the donors who support the Directors' Fund.



Keystone Symposia thanks our Sponsor for generously supporting this meeting:

Pfizer Inc.

We gratefully acknowledge additional support from these exhibitors at this conference:

Thermo Fisher Scientific
Please stop by to meet these exhibitors during the conference.


We gratefully acknowledge additional support for this conference from:

Direct Electron, LP MiTeGen

We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:


Click here to view more of these organizations


Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:


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If your organization is interested in joining these entities in support of Keystone Symposia, please contact: Sarah Lavicka, Director of Development, Email: sarahl@keystonesymposia.org,
Phone:+1 970-262-2690

Click here for more information on Industry Support and Recognition Opportunities.

If you are interested in becoming an advertising/marketing in-kind partner, please contact:
Yvonne Psaila, Director, Marketing and Communications, Email: yvonnep@keystonesymposia.org,
Phone:+1 970-262-2676