Vector-borne diseases pose a significant threat to human health worldwide as highlighted by the increasing incidence and sustained transmission of malaria, dengue and other arboviral diseases, Lyme disease, and trypanosomiasis. Vaccines and therapeutics are limited or not available for these diseases and traditional vector control methods have proven insufficient. Gaining a deeper understanding of the mechanisms that govern vector-pathogen interactions could lead to novel control strategies targeting critical processes within these systems. Recognizing this need, the Keystone symposium on vector-pathogen interactions will provide a crucial opportunity for scientists in vector biology, virology, bacteriology and parasitology to collaborate, exchange scientific advancements and address existing challenges across a variety of biological systems. Furthermore, the symposium aims to cultivate a supportive atmosphere for new and junior researchers entering this field of research. The meeting's agenda includes research breakthroughs on the interactions between diverse vectors, such as mosquitoes, ticks, and tsetse flies, and the viral, parasitic, and bacterial microorganisms they harbor and transmit. This conference brings together experienced and junior scientists to discuss the latest developments in cellular and molecular biology, development, physiology, immunity, microbiota and genomics applied to the field of vector biology, with a focus on the mechanistic principles underpinning novel control strategies.