Mar 23–26, 2025 | Keystone Resort, Keystone, CO, United States
Scientific Organizers:
Elizabeth Villa, Bridget Carragher, and Ilaria Testa

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Scientific Organizers: Elizabeth Villa, Bridget Carragher, and Ilaria Testa
Elizabeth Villa
Bridget Carragher
Ilaria Testa
***Meeting program subject to change.
Available Formats: = In Person = On DemandFundraising
Sunday, March 23, 2025
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Monday, March 24, 2025
Session Chair
Session Chair
Cryo-Electron Tomography or the Power of Seeing the Whole Picture
Session Chair
Have Protein Language Models Learned Dynamics
Hacking AlphaFold to Do New Things
Understanding How AlphaFold Learns
Short Talk: Predicting the Conformational Flexibility of Antibody and T-cell Receptor CDRs
Short Talk: Improving Cryo-EM of Vesicle-Embedded Membrane Proteins: A Machine Learning Pipeline for Lipid Bilayer Subtraction
Session Chair
3D Cell Mapping of Insulin Secretory Granule Maturation using Soft X-ray Tomography
Bringing it All Together: 3D Animation for Integrative Modeling of Complex Molecular Mechanisms
Cloneable Inorganic Nanoparticles
Multiplexed Super-resolution Imaging of Cells and Clinical Tissue Sections from Nanometer to Millimeter Scales
The Machine Learning Challenge for CryoET Annotation: A report on the Winning Models
Structural Analysis of Lysosomal Proteins via Cryo-Electron Tomography of Affinity-Captured Organelles
Imaging DNA Repair Proteins, One Molecule at a Time
Enabling High-Resolution Structure Determination In Situ
Short Talk: Cryo-ET Reveals Novel Assemblies in Olfactory Cilia
Short Talk: Towards Structurally Characterizing Nitrosomonas Europaea Enzymes in vivo for Wastewater Nitrogen Refinement
Regulation of LRRK2: Identifying Vulnerabilities for Parkinson’s Disease Therapeutics
Session Chair
Session Chair
Session Chair
Session Chair
Tuesday, March 25, 2025
Session Chair
Tools and Technologies for Accelerating cryoET
Single Molecule Localization and Structure Probing in Cells
Discovering New Biology with Cryo-ET
Structural Insights into CRISPR-Based Genome Editing
Short Talk: Automated Filtering of Particle Images in Single Particle CryoEM
Assistant Professor
Director of Scientific Projects
Head of Stem Cell Biology
Scientific Editor, Cell Systems
Unveiling the Architecture of the Cell by Combining cryoET and Ultrastructure Expansion Microscopy
Predictions and Probes of Membrane Structure
Soft X-Ray Tomography: Unraveling the Mysteries of Cellular Biochemistry
Short Talk: Time-resolved High-pressure Freezing for Dynamic, Nanoscale Imaging
Wednesday, March 26, 2025
Linking the Structure, Mechanics, and Function of PIEZO Ion Channels with Fluorescence Nanoscopy
From Cells to STARSS: Monitoring the Life of Proteins at the Nanoscale
All-Optical Super-Resolution Imaging of Molecules in Their Nanoscale Cellular Context
Accessing the Nanoscale with Light Microscopy
Correlating Cryogenic Light and Electron Microscopy at the Nanoscale
Short Talk: Illuminating RNA in Living Mammalian Cells: NIR Aptamer Advances for Super-Resolved Imaging
SOLIST: Unlocking Molecular Landscapes Across Scales
VIS-À-VIS: Visualization and Vitrification after Stimulation
High throughput Real-time Reconstruction of Fully Rorrected and Contrast Enhanced cryoET Tomograms
EasyGrid: A Versatile Platform for Automated Cryo-EM Sample Preparation and Control
Improved Cryo-EM Reconstruction of Sub-50 kDa Complexes using 2DTM
Expanding the ToolKit for Cellular Tomography: Delivery into Live Cells of a cryo-CLEM Nanogold Tag for Structural Cell Biology
Session Chair
Mitochondrial Dynamic and Structural States Underpinning Homeostasis
DNA-PAINTs, SOMAmers and Ultra-Resolution Fluorescence Localizomics of DNA, RNA and Protein Interactions
Short Talk: Co-Localization and Dynamics of Multiple Fluorescent Labels at the Molecular Scale
Thursday, March 27, 2025
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